Prognostic Significance of Preoperative and Postoperative Complement C3 Depletion in Gastric Cancer: A Three-Year Survival Investigation

Objectives. The role of complement system in predicting prognosis of gastric cancer (GC) remains obscured. This study aims to explore the incidence of complement C3 depletion and associated outcomes in GC patients. Methods. between August 2013 and December 2013, 106 patients with gastric adenocarcinoma were prospectively analyzed. Plasma levels of complement C3 and C4 were detected at baseline, one day before surgery, and postoperative day 3, respectively. Patients with low C3 levels (<0.75 mg/mL) were considered as having complement depletion (CD), while others with normal C3 levels were included as control. The 3-year overall survival (OS), disease-free survival (DFS), and other outcomes were compared between both groups, with the CD incidence explored meanwhile. Results. The CD incidence was 28.3% before surgery but increased to 37.7% after surgery. Preoperative CD was related to prolonged hospital stay (22.7 versus 19.2 day, P=0.032) and increased postoperative complications (33.3% versus 14.5%, P=0.030) and hospital costs (P=0.013). Besides, postoperative C3 depletion was significantly associated with decreased 3-year OS (P=0.022) and DFS (P=0.003). Moreover, postoperative C3 depletion and advanced tumor stage were independent predictive factors of poor prognosis. Conclusions. Complement C3 depletion occurring in gastric cancer was associated with poor short-term and long-term outcomes.

Download Full-text

Dysregulation of ECRG4 is associated with malignant properties and of prognostic importance in human gastric cancer

Cancer Biomarkers ◽

10.3233/cbm-210334 ◽

2021 ◽

pp. 1-12

Author(s):

Yanjie You ◽

Shengjuan Hu

Keyword(s):

Gastric Cancer ◽

Prognostic Significance ◽

Statistical Analyses ◽

Human Gastric Cancer ◽

Advanced Tumor Stage ◽

Protein Levels ◽

Cancer Pathogenesis ◽

Advanced Tumor ◽

Cancer Tissues ◽

The Impact

BACKGROUND: We have previously characterized esophageal carcinoma-related gene 4 (ECRG4) as a novel tumor suppressor gene, which is frequently inactivated in nasopharyngeal carcinoma and breast cancer. Nevertheless, the expression status and prognostic significance of ECRG4 maintain elusive in human gastric cancer. Herein, we examined ECRG4 expression profile in gastric cancer and assessed its association with clinicopathological characteristics and patient survival. METHODS: Online data mining, real-time RT-PCR and immunohistochemistry were employed to determined ECRG4 expression at transcriptional and protein levels in tumors vs. noncancerous tissues. Statistical analyses including the Kaplan-Meier survival analysis and the Cox hazard model were utilized to detect the impact on clinical outcome. Moreover, ECRG4 expression was silenced in gastric cancer SGC7901 cells, and cell proliferation, colony formation and invasion assays were carried out. RESULTS: ECRG4 mRNA and protein levels were obviously downregulated in cancer tissues than noncancerous tissues. Statistical analyses demonstrated that low ECRG4 expression was found in 34.5% (58/168) of primary gastric cancer tissues, which was associated with higher histological grade (P= 0.018), lymph node metastasis (P= 0.011), invasive depth (P= 0.020), advanced tumor stage (P= 0.002) and poor overall survival (P< 0.001). Multivariate analysis showed ECRG4 expression is an independent prognostic predictor (P< 0.001). Silencing ECRG4 expression promoted gastric cancer cell growth and invasion. Western blot analysis revealed the anti-metastatic functions of ECRG4 by downregulating of E-cadherin and α-Catenin, as well as upregulating N-cadherin and Vimentin. CONCLUSIONS: Our observations reveal that ECRG4 expression is involved in gastric cancer pathogenesis and progression, and may serve as a candidate prognostic biomarker for this disease.

Download Full-text

Prognostic and clinicopathological significance of neutrophil-to-lymphocyte ratio in patients with oral cancer

Bioscience Reports ◽

10.1042/bsr20181550 ◽

2018 ◽

Vol 38 (6) ◽

Cited By ~ 6

Author(s):

Yun Yang ◽

Rongxun Liu ◽

Feng Ren ◽

Rui Guo ◽

Pengfei Zhang

Keyword(s):

Oral Cancer ◽

Meta Analysis ◽

Prognostic Significance ◽

Tumor Stage ◽

Neutrophil To Lymphocyte Ratio ◽

Advanced Tumor Stage ◽

Lymphocyte Ratio ◽

Advanced Tumor ◽

Oral Cancer Patients ◽

Clinicopathological Significance

Objectives: Many studies have examined the prognostic significance of the neutrophil-to-lymphocyte ratio (NLR) in oral cancer; however, the results are contradictory. We, therefore, conducted a meta-analysis aiming to clarify the prognostic value of the NLR in oral cancer patients. Methods: A literature search was conducted in the PubMed, Web of Science, and Embase databases. Stata version 12.0 was used for statistical analysis. Results: A total of 14 studies with 3216 patients were finally included. The results indicated that a high NLR was significantly associated with worse DFS (n=10, HR = 1.73, 95% confidence interval [CI] = 1.44–2.07, P<0.001). Similar results were observed for overall survival (OS) (n=9, HR = 1.61, 95% CI = 1.39–1.86, P<0.001). Moreover, a high NLR was also correlated with lymph node metastasis (n=7, odds ratio [OR] = 1.62, 95% CI = 1.32–1.98, P<0.001), advanced tumor stage (n=7, OR = 2.63, 95% CI = 2.12–3.25, P<0.001), T stage (n=6, OR = 3.22, 95% CI = 2.59–4.01, P<0.001), tumor differentiation (n=5, OR = 1.48, 95% CI = 1.03–2.11, P=0.033), and perineural invasion (n=4, OR = 1.83, 95% CI = 1.4–2.39, P<0.001). However, an elevated NLR was not correlated with gender. Conclusion: This meta-analysis showed that the NLR might be a potential independent prognostic factor in patients with oral cancer.

Download Full-text

Incidence and Prognostic Significance of PD-L1 Expression in High-Grade Salivary Gland Carcinoma

Frontiers in Oncology ◽

10.3389/fonc.2021.701181 ◽

2021 ◽

Vol 11 ◽

Author(s):

Qigen Fang ◽

Yao Wu ◽

Wei Du ◽

Xu Zhang ◽

Defeng Chen

Keyword(s):

Salivary Gland ◽

Tumor Cells ◽

Prognostic Significance ◽

Disease Free Survival ◽

Tumor Stage ◽

Advanced Tumor Stage ◽

High Grade ◽

Salivary Gland Carcinoma ◽

Gland Carcinoma ◽

The Difference

ObjectivePD-L1 is one of the predictors of immunotherapy efficacy. Our goal was to analyze its expression and prognostic significance in high-grade salivary gland carcinoma (SGC).MethodsPD-L1 expression was evaluated using paraffin-embedded specimens from patients with surgically treated high-grade SGC, and it was scored by the tumor proportion score (TPS), combined positive score (CPS), and immune cell (IC) score. Associations between clinicopathological variables, disease-free survival (DFS), overall survival (OS) and PD-L1 expression were assessed.ResultsTPS≥1% occurred in 47 patients with an incidence of 43.1%, and it was significantly related to an advanced tumor stage. In patients with TPS<1%, TPS ranging from 1% to 20%, and TPS≥20%, the 5-year DFS rates were 36%, 26%, and 13%, respectively, and the difference was significant. In patients with TPS<1%, TPS ranging from 1% to 20%, and TPS≥20%, the 5-year OS rates were 49%, 24%, and 13%, respectively, and the difference was significant. CPS≥1 occurred in 87 patients with an incidence of 79.8%. IC scores of 0, 1, 2, and 3 were noted in 24 (22.0%), 37 (33.9%), 31 (28.4%), and 17 (15.6%) patients, respectively. Both CPS and IC scores had no impact on DFS or OS.ConclusionsThe expression of PD-L1 in tumor cells of high-grade SGCs was not uncommon, and it was significantly associated with tumor stage. PD-L1 expression in tumor cells rather than in immune cells indicated a poor prognosis.

Download Full-text

Overexpression of BUB1B, CCNA2, CDC20, and CDK1 in tumor tissues predicts poor survival in pancreatic ductal adenocarcinoma

Bioscience Reports ◽

10.1042/bsr20182306 ◽

2019 ◽

Vol 39 (2) ◽

Cited By ~ 11

Author(s):

Shu Dong ◽

Fei Huang ◽

Hao Zhang ◽

Qiwen Chen

Keyword(s):

Pancreatic Ductal Adenocarcinoma ◽

Tumor Development ◽

Disease Free Survival ◽

Tumor Stage ◽

Gene Expression Omnibus ◽

Ductal Adenocarcinoma ◽

Advanced Tumor Stage ◽

Kaplan Meier ◽

Advanced Tumor ◽

Tumor Tissues

AbstractOverexpressed genes in tumors usually contributed to aggressiveness in pancreatic ductal adenocarcinoma (PDAC). Using Gene Expression Omnibus (GEO) profiles including GSE46234, GSE71989, and GSE107610, we detected overexpressed genes in tumors with R program, which were enriched by Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene ontology (GO), and Reactome pathway databases. Then, we performed a survival analysis of enriched genes based on TCGA profile. Our results revealed that high BUB1B, CCNA2, CDC20, and CDK1 expression in tumors was significantly associated with worse overall survival (OS) (Log rank P=0.00338, P=0.0447, P=0.00965, and P=0.00479, respectively), which was validated using a Kaplan–Meier plotter with a median cutoff (Log rank P=0.028, P=0.0035, P=0.039, and P=0.0033, respectively). Moreover, overexpression of BUB1B, CCNA2, CDC20, and CDK1 in tumor tissues was significantly associated with disease-free survival (DFS) in PDAC patients (Log rank P=0.00565, P=0.0357, P=0.00104, and P=0.00121, respectively). BUB1B, CCNA2, CDC20, and CDK1 were significantly overexpressed in deceased PDAC patients (all P<0.01) and in patients with recurrence/disease progression (all P<0.05). In addition, PDAC patients with neoplasms of histologic grade G3-4 had significantly higher BUB1B, CCNA2 and CDC20 levels (all P<0.05). In conclusion, the up-regulation of BUB1B, CCNA2, CDC20, CDK1, and WEE1 in tumor tissues are associated with worse OS and DFS in PDAC and is correlated with advanced tumor stage and tumor development.

Download Full-text

Overexpressed Proteins in HCC Cell-Derived Exosomes, CCT8, and Cofilin-1 Are Potential Biomarkers for Patients with HCC

Diagnostics ◽

10.3390/diagnostics11071221 ◽

2021 ◽

Vol 11 (7) ◽

pp. 1221

Author(s):

Hyo Jung Cho ◽

Geum Ok Baek ◽

Moon Gyeong Yoon ◽

Hye Ri Ahn ◽

Ju A Son ◽

...

Keyword(s):

Area Under The Curve ◽

Disease Free Survival ◽

Tumor Stage ◽

Serum Markers ◽

Advanced Tumor Stage ◽

Protein Markers ◽

Poor Overall Survival ◽

Advanced Tumor ◽

Normal Hepatocyte ◽

Potential Biomarkers

Protein markers of hepatocellular carcinoma (HCC)-derived exosomes (HEX) have not yet been fully evaluated. Here, we identified novel protein contents of HEX and their clinical significance as biomarkers. Exosomes were isolated from human HCC cell lines and an immortalized normal hepatocyte cell line. Proteomic analyses revealed 15 markedly overexpressed proteins in HEX. The clinical relevance of the 15 proteins was analyzed in public RNA-sequencing datasets, and 6 proteins were selected as candidate of potential biomarkers. Serum CCT8 and CFL1 were markedly overexpressed in test cohort (n = 8). In the validation cohort (n = 224), the area under the curve (AUC) of serum CCT8 and CFL1 for HCC diagnosis was calculated as 0.698 and 0.677, respectively, whereas that of serum alpha-fetoprotein (AFP) was 0.628. The combination of three serum markers (CCT8, CFL1, and AFP) demonstrated the highest AUC for HCC diagnosis. (AUC = 0.838, 95% confidence interval = 0.773–0.876) Furthermore, higher serum CCT8 and CFL1 concentrations were significantly associated with the presence of vascular invasion, advanced tumor stage, poor disease-free survival, and poor overall survival. Cofilin-1 and CCT8, enriched proteins in HEX, were identified as potential diagnostic and prognostic serum biomarkers for HCC patients.

Download Full-text

VPAC1 couples with TRPV4 channel to promote calcium-dependent gastric cancer progression

10.1101/360404 ◽

2018 ◽

Author(s):

Bo Tang ◽

Jilin Wu ◽

Michael X. Zhu ◽

Xuemei Sun ◽

Jingjing Liu ◽

...

Keyword(s):

Gastric Cancer ◽

Cancer Progression ◽

Positive Feedback ◽

Feedback Regulation ◽

Tumor Stage ◽

Regulation Mechanism ◽

Advanced Tumor Stage ◽

Dependent Manner ◽

Advanced Tumor ◽

Gastric Cancer Progression

AbstractAlthough VPAC1 and its ligand vasoactive intestinal peptide (VIP) are important in gastrointestinal physiology, their involvements in progression of gastrointestinal tumor have not been explored. Here, we found that higher expression of VIP/VPAC1 was observed in gastric cancer compared to the adjacent normal tissues. The increased expression of VIP/VPAC1 in gastric cancer correlated positively with invasion, tumor stage, lymph node, distant metastases, and poor survival. Moreover, high expression of VIP and VPAC1, advanced tumor stage and distant metastasis were independent prognostic factors. VPAC1 activation by VIP markedly induced TRPV4-mediated Ca2+ entry, and eventually promoted gastric cancer progression in a Ca2+ signaling-dependent manner. Inhibition of VPAC1 and its signaling pathway could block the progressive responses. VPAC1/TRPV4/Ca2+ signaling in turn enhanced the expression and secretion of VIP in gastric cancer cells, enforcing a positive feedback regulation mechanism. Taken together, our study demonstrate that VPAC1 is significantly overexpressed in gastric cancer and VPAC1/TRPV4/Ca2+ signaling axis could enforce a positive feedback regulation in gastric cancer progression. VIP/VPAC1 may serve as potential prognostic markers and therapeutic targets for gastric cancer.

Download Full-text

Mucinous Gastric Cancer Presents with More Advanced Tumor Stage and Weaker β-Catenin Expression than Nonmucinous Cancer

Annals of Surgical Oncology ◽

10.1245/s10434-010-1184-z ◽

2010 ◽

Vol 17 (11) ◽

pp. 3053-3058 ◽

Cited By ~ 12

Author(s):

Min-Gew Choi ◽

Chang Ohk Sung ◽

Jae Hyung Noh ◽

Kyoung-Mee Kim ◽

Tae Sung Sohn ◽

...

Keyword(s):

Gastric Cancer ◽

Tumor Stage ◽

Advanced Tumor Stage ◽

Advanced Tumor

Download Full-text

The predictive value of lncRNA MIR31HG expression on clinical outcomes in patients with solid malignant tumors

10.21203/rs.2.22773/v1 ◽

2020 ◽

Author(s):

Chao Tu ◽

Xiaolei Ren ◽

Jieyu He ◽

Shuangqing Li ◽

Lin Qi ◽

...

Keyword(s):

Lung Cancer ◽

Clinical Outcomes ◽

Gastrointestinal Cancer ◽

Prognostic Value ◽

Malignant Tumors ◽

Disease Free Survival ◽

Tumor Stage ◽

Advanced Tumor Stage ◽

Advanced Tumor ◽

Tcga Dataset

Abstract Background Emerging studies have explored the prognostic value of MIR31HG in cancers, but its role remains elusive. Herein, we aimed to summarize the prognostic potential of MIR31HG in this study. Methods Several databases were searched for literature retrieval on Dec 5, 2019. Overall and subgroup analyses were conducted to measure the relationship between MIR31HG expression and clinical outcomes. Moreover, GEPIA was applied for validation of prognostic value of MIR31HG in tumor patients in TCGA dataset. Results Overall, seventeen studies with 2,573 patients were enrolled. Compared to counterparts, those patients with high MIR31HG expression tended to have shorter RFS. Notably, MIR31HG overexpression predicted unfavorable OS in lung cancer. By contrast, gastrointestinal cancer patients with elevated MIR31HG expression predicted better OS and disease-free survival. Additionally, MIR31HG overexpression was significantly associated with worse clinicopathological features including advanced tumor stage and LNM in lung cancer, but favorable clinical characteristics in gastrointestinal cancer. Moreover, the positive association between MIR31HG and OS in lung cancer was further confirmed in TCGA dataset. Conclusion Overexpression of MIR31HG suggested remarkable association with poor prognosis in terms of OS, tumor stage, and LNM in lung cancer, but favorable prognosis in gastrointestinal cancer. Therefore, MIR31HG may serve as a promising prognostic biomarker in multiple cancers.

Download Full-text