Anti-Inflammatory Activity of Babassu Oil and Development of a Microemulsion System for Topical Delivery

Babassu oil extraction is the main income source in nut breakers communities in northeast of Brazil. Among these communities, babassu oil is used for cooking but also medically to treat skin wounds and inflammation, and vulvovaginitis. This study aimed to evaluate the anti-inflammatory activity of babassu oil and develop a microemulsion system with babassu oil for topical delivery. Topical anti-inflammatory activity was evaluated in mice ear edema using PMA, arachidonic acid, ethyl phenylpropiolate, phenol, and capsaicin as phlogistic agents. A microemulsion system was successfully developed using a Span® 80/Kolliphor® EL ratio of 6 : 4 as the surfactant system (S), propylene glycol and water (3 : 1) as the aqueous phase (A), and babassu oil as the oil phase (O), and analyzed through conductivity, SAXS, DSC, TEM, and rheological assays. Babassu oil and lauric acid showed anti-inflammatory activity in mice ear edema, through inhibition of eicosanoid pathway and bioactive amines. The developed formulation (39% A, 12.2% O, and 48.8% S) was classified as a bicontinuous to o/w transition microemulsion that showed a Newtonian profile. The topical anti-inflammatory activity of microemulsified babassu oil was markedly increased. A new delivery system of babassu microemulsion droplet clusters was designed to enhance the therapeutic efficacy of vegetable oil.

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Use of 18β-glycyrrhetinic acid nanocrystals to enhance anti-inflammatory activity by improving topical delivery

Colloids and Surfaces B Biointerfaces ◽

10.1016/j.colsurfb.2021.111791 ◽

2021 ◽

pp. 111791

Author(s):

Quan Weiyan ◽

Kong Songzhi ◽

Ouyang Qianqian ◽

Tao Jinlong ◽

Lu Sitong ◽

...

Keyword(s):

Topical Delivery ◽

Glycyrrhetinic Acid ◽

Inflammatory Activity ◽

Anti Inflammatory

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Topical Delivery of Rapamycin by Means of Microenvironment-Sensitive Core-Multi-Shell Nanocarriers: Assessment of Anti-Inflammatory Activity in an ex vivo Skin/T Cell Co-Culture Model

International Journal of Nanomedicine ◽

10.2147/ijn.s330716 ◽

2021 ◽

Vol Volume 16 ◽

pp. 7137-7151

Author(s):

Fiorenza Rancan ◽

Xiao Guo ◽

Keerthana Rajes ◽

Polytimi Sidiropoulou ◽

Fatemeh Zabihi ◽

...

Keyword(s):

T Cell ◽

Ex Vivo ◽

Topical Delivery ◽

Inflammatory Activity ◽

Culture Model ◽

Anti Inflammatory

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Anti-Inflammatory Activity of Cnidoscolus aconitifolius (Mill.) Ethyl Acetate Extract on Croton Oil-Induced Mouse Ear Edema

Applied Sciences ◽

10.3390/app11209697 ◽

2021 ◽

Vol 11 (20) ◽

pp. 9697

Author(s):

Eduardo Padilla-Camberos ◽

Omar Ricardo Torres-Gonzalez ◽

Ivan Moises Sanchez-Hernandez ◽

Nestor Emmanuel Diaz-Martinez ◽

Oscar Rene Hernandez-Perez ◽

...

Keyword(s):

Medicinal Plant ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Inflammatory Activity ◽

Therapeutic Effects ◽

Ear Edema ◽

Croton Oil ◽

Mouse Ear ◽

Anti Inflammatory ◽

Mouse Ear Edema

Nowadays, there is a growing interest in the development of medicinal plant-based therapies to diminish the ravages of the inflammatory process related to diseases and tissue damage. Most therapeutic effects of these traditional medicinal plants are owed to their phenolic and antioxidant properties. C. aconitifolius is a traditional medicinal plant in Mexico. Previous characterization reports have stated its high nutritional and antioxidant components. The present study aimed to better understand the biological activity of C. aconitifolius in inflammation response. We developed an ethyl acetate extract of this plant to evaluate its anti-inflammatory capacity and its flavonoid content. The topical anti-inflammatory effect of the ethyl acetate extract of C. aconitifolius was determined by the croton oil-induced mouse ear edema test, while flavonoid detection and concentration were determined by thin layer chromatography and the aluminum chloride colorimetric assay, respectively. Topical application of the extract showed significant inhibition of the induced-ear edema (23.52 and 49.41% for 25 and 50 mg/kg dose, respectively). The extract also exhibited the presence of flavonoids. The finding of the anti-inflammatory activity exerted by the C. aconitifolius and the identification of its active principles may suggest and support its use for inflammation treatment.

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Anti-Inflammatory Activity and Composition ofSenecio salignusKunth

BioMed Research International ◽

10.1155/2013/814693 ◽

2013 ◽

Vol 2013 ◽

pp. 1-4 ◽

Cited By ~ 4

Author(s):

Cuauhtemoc Pérez González ◽

Roberto Serrano Vega ◽

Marco González-Chávez ◽

Miguel Angel Zavala Sánchez ◽

Salud Pérez Gutiérrez

Keyword(s):

Medicinal Plant ◽

Methanol Extract ◽

Chloroform Extract ◽

Inflammatory Activity ◽

Paw Edema ◽

Active Fraction ◽

Ear Edema ◽

Methanol Extracts ◽

Anti Inflammatory ◽

Inflammatory Effect

We investigated the anti-inflammatory activity ofSenecio salignus. This medicinal plant is often used in Mexico for the treatment of fever and rheumatism. Chloroform and methanol extracts of the plant were tested on 12-O-tetradecanoylphorbol-13-acetate- (TPA-) induced edema in mice ears. The methanol extract of the plant inhibited edema by36±4.4% compared with the control, while the chloroform extract exhibited an even greater level of inhibition (64.1%). The chloroform extract was then fractionated, and the composition of the active fraction was determined by GC-MS. The anti-inflammatory activity of this fraction was then tested on TPA-induced ear edema in mice, and we found that the active fraction could inhibit edema by 46.9%. The anti-inflammatory effect of the fraction was also tested on carrageenan-induced paw edema in rats at doses of 100 mg/kg; a58.9±2.8% reduction of the edema was observed 4 h after administration of carrageenan, and the effect was maintained for 5 h.

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Essential Oil of Myrtus communis Inhibits Inflammation in Rats by Reducing Serum IL-6 and TNF-α

Natural Product Communications ◽

10.1177/1934578x1100601034 ◽

2011 ◽

Vol 6 (10) ◽

pp. 1934578X1100601 ◽

Cited By ~ 8

Author(s):

Andrea Maxia ◽

Maria Assunta Frau ◽

Danilo Falconieri ◽

Manvendra Singh Karchuli ◽

Sanjay Kasture

Keyword(s):

Essential Oil ◽

Inflammatory Activity ◽

Myrtus Communis ◽

Ear Edema ◽

Tnf Α ◽

Cotton Pellet ◽

Anti Inflammatory ◽

Factor Α ◽

Necrosis Factor ◽

Serum Tumor Necrosis

The topical anti-inflammatory activity of the essential oil of Myrtus communis L. was studied using croton oil induced ear edema and myeloperoxidase (MPO) activity in mice, and cotton pellet induced granuloma, and serum tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in rats. On topical application, the oil exhibited a significant decrease in the ear edema as well as MPO activity. The oil also inhibited cotton pellet-induced granuloma and serum TNF-α and IL-6. It can be concluded that the essential oil of Myrtus communis reduces leukocyte migration to the damaged tissue and exhibits anti-inflammatory activity.

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Anti-inflammatory activity of Jefea gnaphalioides (a. gray), Astereaceae

BMC Complementary and Alternative Medicine ◽

10.1186/s12906-019-2654-x ◽

2019 ◽

Vol 19 (1) ◽

Cited By ~ 1

Author(s):

Axel Villagómez-Rodríguez ◽

Julia Pérez-Ramos ◽

Ana Laura Esquivel-Campos ◽

Cuauhtémoc Pérez-González ◽

Claudia Angélica Soto-Peredo ◽

...

Keyword(s):

Antioxidant Activity ◽

Inflammatory Activity ◽

Total Phenolic ◽

Oral Toxicity ◽

Alternative Source ◽

Ear Edema ◽

Tnf Α ◽

Dpph Method ◽

Anti Inflammatory ◽

Commercial Kits

Abstract Background Inflammation is a symptom associated with many diseases. This symptom is treated with steroidal and non-steroidal anti-inflammatory drugs, which can cause severe side effects when used as long-term treatments. Natural products are an alternative source of new compounds with anti-inflammatory activity. Jefea gnaphalioides (Astereaceae) (A. Gray) is a plant species used to treat inflammatory problems, in Mexico. This study determined the anti-inflammatory activity and the composition of the methanol extract of Jefea gnaphalioides (MEJG). Methods The extract was obtained by heating the plant in methanol at boiling point for 4 h, and then the solvent was evaporated under vacuum (MEJG). The derivatization of the extract was performed using Bis-(trimethylsilyl) trifluoroacetamide, and the composition was determined by GC-MS. Total Phenols and flavonoids were determined by Folin-Ciocalteu AlCl3 reaction respectively. The antioxidant activity of MEJG was determined by DPPH method. The acute and chronic anti-inflammatory effects were evaluated on a mouse ear edema induced with 12-O-Tetradecanoylphorbol-13-acetate (TPA). Acute oral toxicity was tested in mice at doses of MEJG of 5000, 2500 and 1250 mg/kg. The levels of NO, TNF-α, IL-1β and IL-6 were determinate in J774A.1 macrophages stimulated by Lipopolysaccharide. The production of inflammatory interleukins was measured using commercial kits, and nitric oxide was measured by the Griess reaction. Results The anti-inflammatory activity of MEJG in acute TPA-induced ear edema was 80.7 ± 2.8%. This result was similar to the value obtained with indomethacin (IND) at the same dose (74.3 ± 2.8%). In chronic TPA-induced edema at doses of 200 mg/kg, the inhibition was 45.7%, which was similar to that obtained with IND (47.4%). MEJG have not toxic effects even at a dose of 5000 mg/kg. MEJG at 25, 50, 100 and 200 μg/mL decreased NO, TNF-α, IL-1β and IL-6 production in macrophages stimulated with LPS. The major compounds in MEJG were α-D-Glucopyranose (6.71%), Palmitic acid (5.59%), D-(+)-Trehalose (11.91%), Quininic acid (4.29%) and Aucubin (1.17%). Total phenolic content was 57.01 mg GAE/g and total flavonoid content was 35.26 mg QE/g MEJG had antioxidant activity. Conclusions MEJG has anti-inflammatory activity.

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Comparison of the Anti-Inflammatory Activity of Sodium Acexamate and Zinc Acexamate in Healing Skin Wounds in Rabbits

Pharmacology ◽

10.1159/000138282 ◽

1987 ◽

Vol 34 (5) ◽

pp. 296-300 ◽

Cited By ~ 7

Author(s):

Olivier Guillard ◽

Philippe Masson ◽

Alain Piriou ◽

Jean-Christophe Brugier ◽

Philippe Courtois

Keyword(s):

Inflammatory Activity ◽

Skin Wounds ◽

Anti Inflammatory

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Breaking the Barrier - Potent Anti-Inflammatory Activity following Efficient Topical Delivery of Etanercept using Thermoresponsive Nanogels

Theranostics ◽

10.7150/thno.21668 ◽

2018 ◽

Vol 8 (2) ◽

pp. 450-463 ◽

Cited By ~ 30

Author(s):

M. Giulbudagian ◽

G. Yealland ◽

S. Hönzke ◽

A. Edlich ◽

B. Geisendörfer ◽

...

Keyword(s):

Topical Delivery ◽

Inflammatory Activity ◽

Anti Inflammatory

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In Vitro and In Vivo Wound Healing and Anti-Inflammatory Activities of Babassu Oil (Attalea speciosa Mart. Ex Spreng., Arecaceae)

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2020/8858291 ◽

2020 ◽

Vol 2020 ◽

pp. 1-10

Author(s):

José Alex A. Santos ◽

José Wellinton da Silva ◽

Simone Maria dos Santos ◽

Maria de Fátima Rodrigues ◽

Camila Joyce A. Silva ◽

...

Keyword(s):

Wound Healing ◽

Healing Process ◽

Palm Tree ◽

Amazon Forest ◽

Myeloperoxidase Activity ◽

Skin Wounds ◽

Babassu Oil ◽

Anti Inflammatory

Babassu (Attalea speciosa Mart. ex Spreng., Arecaceae) is a palm tree endemic to Brazil and found mainly in the borders of Amazon forest, where the harvesting of its fruits is an important source of income for more than 300,000 people. Among the communities of coconut breakers women, babassu oil is used in culinary, as fuel, and mostly as medicinal oil for the treatment of skin wounds and inflammation. This study aimed to evaluate in vitro and in vivo the wound healing effects of babassu oil. In vitro, babassu oil increased the migration of L929 fibroblasts, inhibited the production of nitric oxide by LPS-stimulated peritoneal macrophages, and increased the levels of INF-γ and IL-6 cytokines production. In vivo, babassu oil accelerated the healing process in a full-thickness splinted wound model, by an increase in the fibroblasts number, blood vessels, and collagen deposition in the wounds. The babassu oil also increased the recruitment of inflammatory cells into the wound site and showed an anti-inflammatory effect in a chronic ear edema model, reducing ear thickness, epidermal hyperplasia, and myeloperoxidase activity. Thus, these data corroborate the use of babassu oil in folk medicine as a remedy to treat skin wounds.

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