scholarly journals Enhancement of Angiogenesis by Ultrasound-Targeted Microbubble Destruction Combined with Nuclear Localization Signaling Peptides in Canine Myocardial Infarction

2017 ◽  
Vol 2017 ◽  
pp. 1-10
Author(s):  
Jingjing Cui ◽  
Qing Deng ◽  
Qing Zhou ◽  
Sheng Cao ◽  
Nan Jiang ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Gene Delivery ◽  
Cardiac Function ◽  
Delivery System ◽  
Nuclear Localization ◽  
Troponin I ◽  
Gene Transfection ◽  
Gene Delivery System ◽  
Group A ◽  
Canine Myocardial Infarction

Objective. This study aimed to develop a gene delivery system using ultrasound-targeted microbubbles destruction (UTMD) combined with nuclear localization signal (NLS) and investigate its efficacy and safety for therapeutic angiogenesis in canine myocardial infarction (MI) model. Methods. Fifty MI dogs were randomly divided into 5 groups and transfected with Ang-1 gene plasmid: (i) group A: only injection of microbubbles and Ang-1 plasmid; (ii) group B: only UTMD mediated gene transfection; (iii) group C: UTMD combined with classical NLS mediated gene transfection; (iv) group D: UTMD combined with mutational NLS mediated transfection; and (v) group E: UTMD combined with classical NLS in the presence of a nucleus transport blocker. The mRNA and protein expression of Ang-1 gene, microvessel density (MVD) cardiac troponin I (cTnI), and cardiac function were determined after transfection. Results. The expression of mRNA and protein of Ang-1 gene in group C was significantly higher than that of the other groups (all P < 0.01). The MVD of group C was 10.2-fold of group A and 8.1-fold of group E (P < 0.01). The cardiac function in group C was significant improvement without cTnI rising. Conclusions. The gene delivery system composed of UTMD and NLS is efficient and safe.

Dual-functionalized calcium carbonate based gene delivery system for efficient gene delivery

RSC Advances ◽  
2014 ◽  
Vol 4 (73) ◽  
pp. 38623-38629 ◽  
Author(s):  
Chao-Qun Wang ◽  
Meng-Qing Gong ◽  
Jin-Long Wu ◽  
Ren-Xi Zhuo ◽  
Si-Xue Cheng
Keyword(s):  
Calcium Carbonate ◽  
Gene Delivery ◽  
Delivery System ◽  
Gene Transfection ◽  
Cell Penetrating Peptide ◽  
Gene Delivery System ◽  
Dna Nanoparticles ◽  
Efficient Gene ◽  
Cell Penetrating ◽  
A Cell

Dual-functionalized KALA/PS/CaCO3/DNA nanoparticles containing a cell penetrating peptide (KALA) and protamine sulfate (PS) could effectively mediate gene transfection at a low DNA concentration.

Micelle-like luminescent nanoparticles as a visible gene delivery system with reduced toxicity

2015 ◽  
Vol 3 (42) ◽  
pp. 8394-8400 ◽  
Author(s):  
Keni Yang ◽  
Shengliang Li ◽  
Shubin Jin ◽  
Xiangdong Xue ◽  
Tingbin Zhang ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Delivery System ◽  
Gene Transfection ◽  
Gene Delivery System ◽  
Cationic Polymers ◽  
Luminescent Nanoparticles ◽  
Reduced Toxicity

Luminescent nanoparticles (TPEI) were synthesized to tackle the undesired cytotoxicity of cationic polymers and were also used for visible gene transfection.

A bioreducible supramolecular nanoparticle gene delivery system based on cyclodextrin-conjugated polyaspartamide and adamantyl-terminated polyethylenimine

2018 ◽  
Vol 6 (5) ◽  
pp. 797-808 ◽  
Author(s):  
Yunti Zhang ◽  
Qimin Jiang ◽  
Bo Bi ◽  
Luming Xu ◽  
Jia Liu ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Delivery System ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Cellular Internalization ◽  
Gene Delivery System ◽  
Supramolecular Nanoparticles

Reduction degradable Pasp-SS-CD/Ad4-PEI/pDNA supramolecular nanoparticles via host–guest interaction exhibited improved cellular internalization and higher gene transfection efficiency with lower cytotoxicity.

The Combination of Drug or Gene Delivery System Responding to Cellular Signals (D-RECS) and Sonoporation System for Effective and Safe Gene Delivery

2009 ◽  
Vol 1237 ◽  
Author(s):  
Akira Tsuchiya ◽  
Takeshi Mori ◽  
Yuki Naritomi ◽  
Jeong-Hun Kang ◽  
Daisuke Asai ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Regulation ◽  
Gene Delivery ◽  
Delivery System ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Regulation System ◽  
Gene Delivery System

AbstractWe have developed new gene expression-regulating polymer that can activate transgene expression in response to target intracellular signals. Here, we tried applying sonoporation system to this gene regulation system to enhance the gene expression efficacy. Sonoporation is the method for effective gene transfection in vitro and in vivo. Therefore, the method might enhance the transfection efficiency in our polymer and realize an efficient and safe gene delivery system. Results suggested that the combination of our polymer and sonoporation could improve the gene expression compared to the system using only our polymer that transfers genes into cells via endocytosis. It also kept the ability of the gene regulation responding to cellular signals.

scholarly journals Enhanced Transfection of Human Mesenchymal Stem Cells Using a Hyaluronic Acid/Calcium Phosphate Hybrid Gene Delivery System

Polymers ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 798 ◽  
Author(s):  
Jung Eun Lee ◽  
Yue Yin ◽  
Su Yeon Lim ◽  
E. Seul Kim ◽  
Jaeback Jung ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Hyaluronic Acid ◽  
Calcium Phosphate ◽  
Gene Delivery ◽  
Delivery System ◽  
Gene Transfection ◽  
Human Mesenchymal Stem Cells ◽  
Bone Morphogenetic Protein 2 ◽  
Gene Delivery System

Human mesenchymal stem cells (hMSCs) show enormous potential in regenerative medicine and tissue engineering. However, current use of hMSCs in clinics is still limited because there is no appropriate way to control their behavior in vivo, such as differentiation to a desired cell type. Genetic modification may provide an opportunity to control the cells in an active manner. One of the major hurdles for genetic manipulation of hMSCs is the lack of an efficient and safe gene delivery system. Herein, biocompatible calcium phosphate (CaP)-based nanoparticles stabilized with a catechol-derivatized hyaluronic acid (dopa-HA) conjugate were used as a carrier for gene transfection to hMSCs for improved differentiation. Owing to the specific interactions between HA and CD44 of bone marrow-derived hMSCs, dopa-HA/CaP showed significantly higher transfection in hMSCs than branched polyethylenimine (bPEI, MW 25 kDa) with no cytotoxicity. The co-delivery of a plasmid DNA encoding bone morphogenetic protein 2 (BMP-2 pDNA) and micro RNA 148b (miRNA-148b) by dopa-HA/CaP achieved significantly improved osteogenic differentiation of hMSCs.

Construction of traceable cucurbit[7]uril-based virus-mimicking quaternary complexes with aggregation-induced emission for efficient gene transfection

2020 ◽  
Vol 8 (33) ◽  
pp. 7475-7482
Author(s):  
De-E Liu ◽  
Xiangjie Yan ◽  
Jinxia An ◽  
Jianbiao Ma ◽  
Hui Gao
Keyword(s):  
Gene Delivery ◽  
Delivery System ◽  
Gene Transfection ◽  
Delivery Systems ◽  
Endosomal Escape ◽  
Gene Delivery System ◽  
Aggregation Induced Emission ◽  
Efficient Gene ◽  
Novel Virus

A novel virus-mimicking gene delivery system with excellent endosomal escape, efficient transfection, and traceability is developed which can serve as a reference for the construction of novel multifunctional efficient gene delivery systems.

Sign in / Sign up

Export Citation Format

Share Document