Effect of Resveratrol Dry Suspension on Immune Function of Piglets

Resveratrol, a polyphenolic plant antitoxin, has a wide range of pharmacological activities. In this study, we systematically evaluated the effects of resveratrol dry suspension (RDS) on immune function in piglets that were treated with different doses of RDS for 2 weeks. The results showed that the RDS has significant effects on the development, maturation, proliferation, and transformation of T lymphocytes. RDS could regulate humoral immune responses by upregulating the release of IFN-γ and downregulating the release of TNF-α. After piglets were vaccinated against classical swine fever virus and foot-and-mouth disease virus, the antibody titers were significantly increased. RDS treatment showed an excellent resistance to enhance T-SOD activity. Values of blood routine and blood biochemistry showed no toxicity. These results suggested that RDS could be considered as an adjuvant to enhance immune responses to vaccines, as well as dietary additives for animals to enhance humoral and cellular immunity.

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Need for cellular and humoral immune responses in bovines to ensure protection from foot-and-mouth disease virus (FMDV)—A point of view

Virus Genes ◽

10.1007/bf01703078 ◽

1994 ◽

Vol 8 (2) ◽

pp. 199-214 ◽

Cited By ~ 21

Author(s):

Yechiel Becker

Keyword(s):

Immune Responses ◽

Disease Virus ◽

Foot And Mouth Disease ◽

Mouth Disease ◽

Point Of View ◽

Humoral Immune ◽

Humoral Immune Responses ◽

Mouth Disease Virus ◽

Foot And Mouth

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The effect of molybdenum, iron and sulphur supplementation on immune function in growing lambs

Proceedings of the British Society of Animal Science ◽

10.1017/s1752756200008590 ◽

2002 ◽

Vol 2002 ◽

pp. 203-203

Author(s):

C.L. Williams ◽

A.M. Mackenzie ◽

R.G. Wilkinson

Keyword(s):

Immune Responses ◽

Immune Function ◽

Infection Rate ◽

Copper Deficiency ◽

Differential Effects ◽

Humoral Immune ◽

Humoral Immune Responses ◽

Cu Deficiency ◽

Ruminant Animals ◽

Antagonistic Interactions

Clinical copper (Cu) deficiency in ruminant animals is mainly attributed to antagonistic interactions with molybdenum (Mo), iron (Fe) and sulphur (S) and Williams et al. (2001) reported differential effects between Mo and S, compared with Fe and S on various copper parameters. Suttle and Jones (1986) reported an increased mortality of lambs and infection rate due to copper deficiency which suggests an effect of Cu deficiency on immune function. The aim of this study was to assess the effects of Mo or Fe and S on the cellular and humoral immune responses of growing lambs.

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Increased Humoral Immune Responses of Pigs to Foot-and-Mouth Disease Vaccine Supplemented with Ginseng Stem and Leaf Saponins

Chemistry & Biodiversity ◽

10.1002/cbdv.201100377 ◽

2012 ◽

Vol 9 (10) ◽

pp. 2225-2235 ◽

Cited By ~ 12

Author(s):

Yutao Li ◽

Feng Xie ◽

Jian Chen ◽

Qing Fan ◽

Lijuan Zhai ◽

...

Keyword(s):

Immune Responses ◽

Foot And Mouth Disease ◽

Mouth Disease ◽

Humoral Immune ◽

Humoral Immune Responses ◽

Foot And Mouth

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Optimization of Expression and Purification of Recombinant Mouse plac1

Avicenna Journal of Medical Biotechnology ◽

10.18502/ajmb.v14i1.8171 ◽

2022 ◽

Author(s):

Shaghayegh Rahdan ◽

Seyed Alireza Razavi ◽

Mahboobeh Nazari ◽

Sorour Shojaeian ◽

Fazel Shokri ◽

...

Keyword(s):

Immune Responses ◽

Recombinant Proteins ◽

Culture Medium ◽

Ectopic Expression ◽

Expression And Purification ◽

Humoral Immune ◽

Humoral Immune Responses ◽

Wide Range ◽

Humoral Responses ◽

And Control

Background: Placenta-specific 1 (PLAC1) is one of the recently-discovered Cancer-Testis-Placenta (CTP) antigen with restricted normal tissue and ectopic expression in a wide range of cancer cells from different histological origins. The production of recombinant human PLAC1 has already been optimized; however, no study has been reported so far on the production and purification of mouse plac1. In this study, mouse plac1 expression and purification was optimized in a prokaryotic system and the effects of the generated proteins on inducing humoral responses in mice were investigated. Methods: A fusion protein containing full extracellular domain of mouse plac1, immunostimulatory peptides, tetanus toxin P2P30 and PADRE and KDEL3 signal (main plac1), and the same fragment without immunostimulatory peptides (control plac1) was produced. To optimize production and purification steps, different parameters including bacterial strain, cultivation temperature, cultivation time, IPTG concentration, culture medium, and also different buffers for purification of the recombinant proteins were tested. After confirming the identity of recombinant plac1 proteins with Western Blotting (WB) and ELISA assays, these proteins were subcutaneously injected in mice with Freund's adjuvant and the anti-plac1 antibody response was detected by ELISA. Results: The optimal expression level of main and control plac1 was obtained in BL21 (DE3) and TB culture medium in the presence of 0.25 mM IPTG after 24 hr of induction at 15°C. The buffer containing 2% sarkosyl produced higher yield and purity. Our results showed specific reactivity of anti-human recombinant plac1 polyclonal antibody with both main and control plac1 recombinant proteins in WB and ELISA analysis. Both proteins induced humoral responses in mice; however, anti-plac1 antibody titer was significantly higher in sera of mice immunized with main compared to control plac1. Conclusion: In this study, an optimized protocol for production and purification of mouse plac1 was reported and it was shown that insertion of immunostimulatory peptides in gene construct could efficiently enhance humoral immune responses against mouse plac1, which could potentially augment cellular immune responses against plac1 leading to more effective anti-cancer responses.

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Advanced Foot-And-Mouth Disease Vaccine Platform for Stimulation of Simultaneous Cellular and Humoral Immune Responses

Vaccines ◽

10.3390/vaccines8020254 ◽

2020 ◽

Vol 8 (2) ◽

pp. 254

Author(s):

Min Ja Lee ◽

Hyundong Jo ◽

So Hui Park ◽

Mi-Kyeong Ko ◽

Su-Mi Kim ◽

...

Keyword(s):

Immune Response ◽

Immune Responses ◽

Clinical Symptoms ◽

Foot And Mouth Disease ◽

Mouth Disease ◽

Vaccine Platform ◽

Humoral Immune ◽

Humoral Immune Responses ◽

Foot And Mouth ◽

Antibody Titers

Currently available commercial foot-and-mouth disease (FMD) vaccines have various limitations, such as the slow induction and short-term maintenance of antibody titers. Therefore, a novel FMD vaccine that can rapidly induce high neutralizing antibody titers to protect the host in early stages of an FMD virus infection, maintain high antibody titers for long periods after one vaccination dose, and confer full protection against clinical symptoms by simultaneously stimulating cellular and humoral immunity is needed. Here, we developed immunopotent FMD vaccine strains A-3A and A-HSP70, which elicit strong initial cellular immune response and induce humoral immune response, including long-lasting memory response. We purified the antigen (inactivated virus) derived from these immunopotent vaccine strains, and evaluated the immunogenicity and efficacy of the vaccines containing these antigens in mice and pigs. The immunopotent vaccine strains A-3A and A-HSP70 demonstrated superior immunogenicity compared with the A strain (backbone strain) in mice. The oil emulsion-free vaccine containing A-3A and A-HSP70 antigens effectively induced early, mid-term, and long-term immunity in mice and pigs by eliciting robust cellular and humoral immune responses through the activation of co-stimulatory molecules and the secretion of proinflammatory cytokines. We successfully derived an innovative FMD vaccine formulation to create more effective FMD vaccines.

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Immunology of Diseases Associated with Malassezia Species

Clinical Microbiology Reviews ◽

10.1128/cmr.15.1.21-57.2002 ◽

2002 ◽

Vol 15 (1) ◽

pp. 21-57 ◽

Cited By ~ 182

Author(s):

H. Ruth Ashbee ◽

E. Glyn V. Evans

Keyword(s):

Immune Response ◽

Immune Responses ◽

Reticuloendothelial System ◽

Humoral Immune ◽

Malassezia Species ◽

Humoral Immune Responses ◽

Commensal Flora ◽

Wide Range ◽

Nonspecific Immune Response ◽

Phagocytic Uptake

SUMMARY Malassezia species are members of the human cutaneous commensal flora, in addition to causing a wide range of cutaneous and systemic diseases in suitably predisposed individuals. Studies examining cellular and humoral immune responses specific to Malassezia species in patients with Malassezia-associated diseases and healthy controls have generally been unable to define significant differences in their immune response. The use of varied antigenic preparations and strains from different Malassezia classifications may partly be responsible for this, although these problems can now be overcome by using techniques based on recent work defining some important antigens and also a new taxonomy for the genus. The finding that the genus Malassezia is immunomodulatory is important in understanding its ability to cause disease. Stimulation of the reticuloendothelial system and activation of the complement cascade contrasts with its ability to suppress cytokine release and downregulate phagocytic uptake and killing. The lipid-rich layer around the yeast appears to be pivotal in this alteration of phenotype. Defining the nonspecific immune response to Malassezia species and the way in which the organisms modulate it may well be the key to understanding how Malassezia species can exist as both commensals and pathogens.

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