Protective Effect of Rosamultin against H2O2-Induced Oxidative Stress and Apoptosis in H9c2 Cardiomyocytes

Rosamultin is one of the main active compounds isolated from Potentilla anserina L., which belongs to a triterpene compound. Few studies have examined the effect of rosamultin on oxidative stress and its molecular mechanism. The aim of this present study was to elucidate the protective effect of rosamultin on H2O2-induced oxidative damage and apoptosis in H9c2 cardiomyocytes and its mechanism. The results showed that the pretreatment of rosamultin not only increased cell viability but also reduced the release of LDH and CK. Rosamultin inhibited a H2O2-induced decrease in SOD, CAT, and GSH-Px activities and an increase in MDA content. Meanwhile, ROS level, intracellular (Ca2+) fluorescence intensity, and apoptosis rate in the rosamultin pretreated group were markedly decreased compared with the model group. Rosamultin pretreatment significantly reversed the morphological changes and attenuated H2O2-induced apoptosis. Western blot analysis showed that rosamultin enhanced the expression of Bcl-2 and pCryAB and downregulated the expression of Bax, Cyt-c, Caspase-3, and Caspase-9 expression. Additionally, rosamultin might activate PI3K/Akt signal pathways and CryAB relative factors. Therefore, we suggest that rosamultin could have the potential for treating H2O2-induced oxidative stress injury through its antioxidant and antiapoptosis effect.

Download Full-text

Protective Effect of L-carnitine on the Oxidative Stress Injury of Human Ovarian Granulosa Cells

10.21203/rs.3.rs-1037009/v1 ◽

2021 ◽

Author(s):

Xuening Li ◽

Xiaodong Wu ◽

Yuemin Zhang ◽

Tianyi Ma ◽

Pingping Sun ◽

...

Keyword(s):

Oxidative Stress ◽

Protective Effect ◽

Cell Viability ◽

Granulosa Cells ◽

Blank Group ◽

Stress Injury ◽

Ovarian Granulosa Cells ◽

Nuclear Pyknosis ◽

Mda Content

Abstract The protective effect of L-carnitine (LC) on the oxidative stress (OS) injury and the effect of L-carnitine on follicular stimulating hormone receptor (FSHR) of ovarian granulosa cells (GCs) were investigated. OS was induced by treatment with H2O2. We cultured KGN cells in four groups: the blank group, OS group and two L-carnitine pretreatment group (low, high). In the OS group, cell nuclear pyknosis was observed, mitochondria swelled irregularly and their cristae were fractured. Meanwhile, the cell viability, superoxide dismutase (SOD) and glutathione (GSH) contents, mitochondrial membrane potential (ΔΨm) and the level of FSHR expression were significantly decreased in the OS group. However, malonaldehyde (MDA) content, reactive oxygen species (ROS) level and apoptosis rate were significantly increased. Compared with the OS group, the morphology of cells and mitochondria in the L-carnitine pretreatment group were improved, the cell viability and the expression of FSHR was significantly increased, and the OS level was decreased. These results indicated that L-carnitine can protect the cells from OS damage induced by H2O2, enhance the antioxidant and anti-apoptotic ability of GCs, and alleviate the decrease of FSHR expression on GCs caused by OS. Therefore, L-carnitine may help prevent the ovarian aging and improve the quality of follicles.

Download Full-text

Protective Effect of Components Isolated from Lindera erythrocarpa against Oxidative Stress-induced Apoptosis of H9c2 Cardiomyocytes

Phytotherapy Research ◽

10.1002/ptr.3465 ◽

2011 ◽

Vol 25 (11) ◽

pp. 1612-1617 ◽

Cited By ~ 15

Author(s):

Jeong Ah Kim ◽

Yi-Sook Jung ◽

Mi-Young Kim ◽

Seo Young Yang ◽

Sohyun Lee ◽

...

Keyword(s):

Oxidative Stress ◽

Protective Effect ◽

H9c2 Cardiomyocytes ◽

Induced Apoptosis

Download Full-text

Protective Effect of Docosahexaenoic Acid on Oxidative Stress-Induced Apoptosis of Retina Photoreceptors

Investigative Opthalmology & Visual Science ◽

10.1167/iovs.02-0901 ◽

2003 ◽

Vol 44 (5) ◽

pp. 2252 ◽

Cited By ~ 94

Author(s):

Nora P. Rotstein ◽

Luis E. Politi ◽

O. Lorena German ◽

Romina Girotti

Keyword(s):

Oxidative Stress ◽

Docosahexaenoic Acid ◽

Protective Effect ◽

Induced Apoptosis

Download Full-text

Hydroxysafflor yellow A attenuates oxidative stress injury‑induced apoptosis in the nucleus pulposus cell line and regulates extracellular matrix balance via CA XII

Experimental and Therapeutic Medicine ◽

10.3892/etm.2021.11105 ◽

2021 ◽

Vol 23 (2) ◽

Author(s):

Shuo Yang ◽

Wenbo Liao

Keyword(s):

Oxidative Stress ◽

Extracellular Matrix ◽

Cell Line ◽

Nucleus Pulposus ◽

Nucleus Pulposus Cell ◽

Hydroxysafflor Yellow A ◽

Stress Injury ◽

Oxidative Stress Injury ◽

Induced Apoptosis

Download Full-text

Human Amnion Membrane Proteins Prevent Doxorubicin-Induced Oxidative Stress Injury and Apoptosis in Rat H9c2 Cardiomyocytes

Cardiovascular Toxicology ◽

10.1007/s12012-020-09564-8 ◽

2020 ◽

Vol 20 (4) ◽

pp. 370-379 ◽

Cited By ~ 1

Author(s):

Yousef Faridvand ◽

Parinaz Haddadi ◽

Vahid Vahedian ◽

Samira Nozari ◽

Hamid Reza Nejabati ◽

...

Keyword(s):

Oxidative Stress ◽

Membrane Proteins ◽

Stress Injury ◽

Human Amnion ◽

Oxidative Stress Injury ◽

H9c2 Cardiomyocytes ◽

Amnion Membrane

Download Full-text

In Vitro and In Vivo Inhibitory Effect of Citrus Junos Tanaka Peel Extract against Oxidative Stress-Induced Apoptotic Death of Lung Cells

Antioxidants ◽

10.3390/antiox9121231 ◽

2020 ◽

Vol 9 (12) ◽

pp. 1231

Author(s):

Jin Woo Kim ◽

Eun Hee Jo ◽

Ji Eun Moon ◽

Hanvit Cha ◽

Moon Han Chang ◽

...

Keyword(s):

Oxidative Stress ◽

Protective Effect ◽

Inhibitory Effect ◽

Lung Cells ◽

In Vivo Models ◽

Citrus Junos ◽

Induced Apoptosis ◽

Peel Extract

Various stresses derived from both internal and external oxidative environments lead to the excessive production of reactive oxygen species (ROS) causing progressive intracellular oxidative damage and ultimately cell death. The objective of this study was to evaluate the protective effects of Citrus junos Tanaka peel extract (CE) against oxidative-stress induced the apoptosis of lung cells and the associated mechanisms of action using in vitro and in vivo models. The protective effect of CE was evaluated in vitro in NCI-H460 human lung cells exposed to pro-oxidant H2O2. The preventive effect of CE (200 mg/kg/day, 10 days) against pulmonary injuries following acrolein inhalation (10 ppm for 12 h) was investigated using an in vivo mouse model. Herein, we demonstrated the inhibitory effect of CE against the oxidative stress-induced apoptosis of lung cells under a highly oxidative environment. The function of CE is linked with its ability to suppress ROS-dependent, p53-mediated apoptotic signaling. Furthermore, we evaluated the protective role of CE against apoptotic pulmonary injuries associated with the inhalation of acrolein, a ubiquitous and highly oxidizing environmental respiratory pollutant, through the attenuation of oxidative stress. The results indicated that CE exhibits a protective effect against the oxidative stress-induced apoptosis of lung cells in both in vitro and in vivo models.

Download Full-text

Protective Effect of Puerarin Against Oxidative Stress Injury of Neural Cells and Related Mechanisms

Medical Science Monitor ◽

10.12659/msm.896058 ◽

2016 ◽

Vol 22 ◽

pp. 1244-1249 ◽

Cited By ~ 16

Author(s):

Yuan Cheng ◽

Wei Leng ◽

Jingshu Zhang

Keyword(s):

Oxidative Stress ◽

Protective Effect ◽

Neural Cells ◽

Stress Injury ◽

Oxidative Stress Injury

Download Full-text

Stress response and toxicity studies on zebrafish exposed to endosulfan and imidacloprid present in water

Journal of Water Supply Research and Technology—AQUA ◽

10.2166/aqua.2019.077 ◽

2019 ◽

Vol 68 (8) ◽

pp. 718-730 ◽

Cited By ~ 1

Author(s):

Barzah Muazzam ◽

Kashif Munawar ◽

Imtiaz Ahmad Khan ◽

Sarwat Jahan ◽

Mazhar Iqbal ◽

...

Keyword(s):

Oxidative Stress ◽

Dna Damage ◽

Deoxyribonucleic Acid ◽

Morphological Changes ◽

Agricultural Runoff ◽

Control Treatment ◽

Low Concentrations ◽

Mda Content ◽

Combined Exposures ◽

High Concentrations

Abstract Fish and other aquatic biota are hampered by mixtures of pesticides which pollute natural water through agricultural runoff and other sources. Toxicity of combined exposures of endosulfan and imidacloprid on zebrafish in terms of oxidative stress and deoxyribonucleic acid (DNA) damage in liver and histological alterations in gills and muscles was investigated. Zebrafish were exposed to three different sub-lethal concentrations of endosulfan and imidacloprid along with control selected for each treatment for 21 days: control treatment (CT), treatment 1 (T1), treatment 2 (T2) and treatment 3 (T3). T1, T2 and T3 groups were exposed to 0.1, 0.5 and 1 μg/L of endosulfan, respectively, while imidacloprid concentration was maintained at 1 ppm in all three treatments. Oxidative stress was evaluated by measuring levels of catalase (CAT), superoxide dismutase (SOD) and malondialdehyde (MDA). Comet assay was applied to measure degree of DNA damage. Dose- and time-dependent decrease in SOD and CAT activity was observed after 21 days of exposure while low concentrations of pesticides induced SOD and CAT activities after early exposure to reduce the oxidative stress. MDA content was found to be increased in T3 having high concentrations of pesticides. Substantial increase in DNA damage was noticed after 21 days' exposure to pesticides. Significant morphological changes were observed in gills relative to muscles.

Download Full-text