scholarly journals Antiobesity and Antioxidant Potentials of Selected Palestinian Medicinal Plants

2018 ◽  
Vol 2018 ◽  
pp. 1-21 ◽  
Author(s):  
Rana M. Jamous ◽  
Salam Y. Abu-Zaitoun ◽  
Rola J. Akkawi ◽  
Mohammed S. Ali-Shtayeh
Keyword(s):  
Antioxidant Activity ◽  
Camellia Sinensis ◽  
Pancreatic Lipase ◽  
Plant Extracts ◽  
Curcuma Longa ◽  
Ceratonia Siliqua ◽  
Mentha Spicata ◽  
Sarcopoterium Spinosum ◽  
Pancreatic Lipase Inhibition ◽  
Lipase Inhibition

We evaluated the antioxidant and porcine pancreatic lipase inhibition (PPLI) activities of 90 plants extracts. The antioxidant activity was measured using the free-radical scavenging capacity (DPPH) and reducing power (RP) assays. The pancreatic lipase inhibition assay was used to determine the PPLI activity of plant extracts. Among the 90 plant extracts examined, 41.0 % crude extracts showed antilipase activity of more than 50%. The most active plants by means of IC50 value were Camellia sinensis (0.5 mg/ml), Ceratonia siliqua (leaves) (0.8 mg/mL), Curcuma longa (0.8 mg/mL), Sarcopoterium spinosum (1.2 mg/mL), and Mentha spicata (1.2 mg/mL). The antioxidant activity of plant extracts using the DPPH and RP assays reveals comparable results. The most active antioxidant extracts using both assays were the leaves and fruit epicarp of Rhus coriaria, areal parts of Sarcopoterium spinosum, and leaves of Ceratonia siliqua. Our results suggest natural resources that possess strong antioxidant and pancreatic lipase inhibitory activities with potential applications in the treatment and prevention of obesity and overweight. The extracts of Camellia sinensis, Ceratonia siliqua, Curcuma longa, Sarcopoterium spinosum, and Mentha spicata were proved to have a great potential as antioxidants and antiobesity agents.

ANTI-DIABESITY PRINCIPLE FROM THE SEEDS OF PHYLLANTHUS EMBLICA L.

INDIAN DRUGS ◽  
2021 ◽  
Vol 57 (12) ◽  
pp. 41-50
Author(s):  
Priyanka Rathod ◽  
Chandana Kulkarni ◽  
Raman P. Yadav
Keyword(s):  
Antioxidant Activity ◽  
Pancreatic Lipase ◽  
Inhibitory Activity ◽  
Methanolic Extract ◽  
Scavenging Activity ◽  
Phyllanthus Emblica ◽  
Natural Substrate ◽  
Glucosidase Inhibitor ◽  
Pancreatic Lipase Inhibition ◽  
Lipase Inhibition

In recent years, pancreatic lipase inhibitor and α- glucosidase inhibitor have been highlighted as potential anti-diabesity principles. In the present study, seeds of Phyllanthus emblica L. (Family: Phyllanthaceae) was studied for anti-diabesity potential in terms of pancreatic lipase inhibitory activity, α-glucosidase inhibitory activity and antioxidant activity. At 100μg/ml concentration, pancreatic lipase inhibition of the methanolic extract using synthetic substrate obtained was 73.2±0.1% (IC50 59.1μg/ml), whereas pancreatic lipase inhibition using natural substrate was 87.9 ± 2.62%. α- glucosidase inhibition of the extract at 50μg/ml was measured as 94.4±0.37% (IC50 34.4μg/ml). The superoxide scavenging activity of the extract was found to be 81.5±0.41%. Interestingly, upon TLC fingerprinting, only one band with Rf 0.70 showed multifunctional activity. The phytochemical found to be present was an alkaloid. The results evidenced the presence of multifunctional smart molecule in methanolic extract of P. emblica L and showed an alkaloid as the component responsible for anti-diabesity potential.

scholarly journals Araçá (Psidium cattleianum Sabine): bioactive compounds, antioxidant activity and pancreatic lipase inhibition

2021 ◽  
Vol 51 (11) ◽  
Author(s):  
Elisa dos Santos Pereira ◽  
Juliana Rocha Vinholes ◽  
Taiane Mota Camargo ◽  
Chirle de Oliveira Raphaelli ◽  
Núbia Marilin Lettnin Ferri ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Pancreatic Lipase ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Biological Activities ◽  
Total Phenolic ◽  
Total Anthocyanins ◽  
Pancreatic Lipase Inhibition ◽  
Lipase Inhibition

ABSTRACT: “Araçá” has been reported with different biological activities such as antioxidant, antiproliferative and antimicrobial as well as inhibitors of digestive enzymes. The digestive pancreatic lipase enzyme plays a fundamental role in lipid metabolism, and its inhibition has been studied as a target for obesity treatment. This study quantified the bioactive compounds present in different parts of “araçá” fruit and evaluated their antioxidant activity and lipase inhibition properties. Three samples were analyzed for total anthocyanins, total phenolic content, antioxidant activity and pancreatic lipase inhibition. Anthocyanins were reported only in pulp-peel of red “araçá” sample. Phenolic compounds concentration was higher in pulp-peel than in seeds for all samples. The antioxidant activity followed the same trend. A positive correlation was observed between total phenolic content and both antioxidant activity and lipase inhibition. Lipase inhibition activity was higher for pulp-peel compared to the seeds. Overall, the results showed that “araçá” fruit extracts could be beneficial for the treatment of obesity.

Screening of selected food and medicinal plant extracts for pancreatic lipase inhibition

2008 ◽  
Vol 23 (6) ◽  
pp. 874-877 ◽  
Author(s):  
Petra Slanc ◽  
Bojan Doljak ◽  
Samo Kreft ◽  
Mojca Lunder ◽  
Damjan Janeš ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Pancreatic Lipase ◽  
Plant Extracts ◽  
Medicinal Plant Extracts ◽  
Pancreatic Lipase Inhibition ◽  
Lipase Inhibition

scholarly journals Biological activity of alginate and its effect on pancreatic lipase inhibition as a potential treatment for obesity

2015 ◽  
Vol 49 ◽  
pp. 18-24 ◽  
Author(s):  
David Houghton ◽  
Matthew D. Wilcox ◽  
Peter I. Chater ◽  
Iain A. Brownlee ◽  
Chris J. Seal ◽  
...  
Keyword(s):  
Biological Activity ◽  
Pancreatic Lipase ◽  
Potential Treatment ◽  
Pancreatic Lipase Inhibition ◽  
Lipase Inhibition

Pharmacophore Mapping of Natural Products for Pancreatic Lipase Inhibition

2020 ◽  
pp. 305-338 ◽  
Author(s):  
Matheus Gabriel de Oliveira ◽  
Waléria Ramos Nogueira de Souza ◽  
Ricardo Pereira Rodrigues ◽  
Daniel F. Kawano ◽  
Leonardo Luiz Borges ◽  
...  
Keyword(s):  
Natural Products ◽  
Pancreatic Lipase ◽  
Pharmacophore Mapping ◽  
Pancreatic Lipase Inhibition ◽  
Lipase Inhibition

scholarly journals Kinetic Behaviour of Pancreatic Lipase Inhibition by Ultrasonicated A. malaccensis and A. subintegra Leaves of Different Particle Sizes

2020 ◽  
Vol 15 (3) ◽  
pp. 818-828
Author(s):  
Miradatul Najwa Muhd Rodhi ◽  
Fazlena Hamzah ◽  
Ku Halim Ku Hamid
Keyword(s):  
Particle Size ◽  
Gallic Acid ◽  
Pancreatic Lipase ◽  
Crude Extract ◽  
Competitive Inhibition ◽  
Treatment Method ◽  
Crude Extracts ◽  
Pre Treatment ◽  
Pancreatic Lipase Inhibition ◽  
Lipase Inhibition

Gallic acid and quercetin equivalent were determined in the crude extract of matured leaves Aquilaria malaccensis and Aquilaria subintegra. The leaves of both Aquilaria species were dried at 60 °C for 24 hours, ground and sieved into particle size of 250, 300, 400, 500, and 1000 µm. Then, each particle size of leaves was soaked in distilled water with a ratio of 1:100 (w/v) for 24 hours and undergoes the pre-treatment method by using ultrasonicator (37 kHz), at the temperature of 60 °C for 30 minutes. The crude extracts were obtained after about 4 hours of hydrodistillation process. The highest concentration of gallic acid and quercetin equivalent was determined in the crude extract from the particle size of 250 µm. The kinetics of pancreatic lipase inhibition was further studied based using the Lineweaver-Burk plot, wherein the concentration of p-NPP as the substrate and pancreatic lipase were varied. Based on the formation of the lines in the plot, the crude leaves extract of both Aquilaria species exhibit the mixed-inhibition on pancreatic lipase, which indicates that in the reaction, the inhibitors were not only attached to the free pancreatic lipase, but also to the pancreatic lipase-(p-NPP) complex. The reaction mechanism was similar to non-competitive inhibition; however the value of dissociation constant, Ki, for both inhibition pathways was different. The inhibition shows an increment in Michaelis-Menten constant (Km) and a reduction in the maximum pancreatic lipase activity (Vm) compared to the reaction without Aquilaria spp. crude extracts (control). This proved that the inhibition occurred in this reaction. Copyright © 2020 BCREC Group. All rights reserved 

scholarly journals In Vitro Pancreatic Lipase Inhibition by Marine Fungi Purpureocillium lilacinum Associated with Stylissa sp. Sponge as Anti-obesity Agent

2021 ◽  
Vol 29 (1) ◽  
pp. 76-86
Author(s):  
Wendi Nurul Fadillah ◽  
Nampiah Sukarno ◽  
Dyah Iswantini ◽  
Min Rahminiwati ◽  
Novriyandi Hanif ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Pancreatic Lipase ◽  
Metabolite Identification ◽  
Molecular Characteristics ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Purpureocillium Lilacinum ◽  
Pancreatic Lipase Inhibition ◽  
Lipase Inhibition

This study aimed to evaluate the potential of marine fungus Purpureocillium lilacinum isolated from an Indonesian marine sponge Stylissa sp. as an anti-obesity agent through pancreatic lipase inhibition assay. The fungus was identified as P. lilacinum through morphological and molecular characteristics. The fungal extract’s inhibition activity and kinetics were evaluated using spectrophotometry and Lineweaver-Burk plots. Ethyl acetate and butanol were used for extraction. Both extracts showed pancreatic lipase inhibition in a concentration-dependent manner. Both crude extracts were then fractionated once. All fractionated extracts showed inhibitory activity above 50%, with the highest activity found in fraction 5 of ethyl acetate at 93.41% inhibition. The best fractionated extract had an IC50value of 220.60 µg.mL-1. The most active fraction of P. lilacinum had a competitive-type inhibitor behavior as shown by the value of Vmax not significantly changing from 388.80 to 382.62 mM pNP.min-1, and the Michaelis-Menten constant (KM) increased from 2.02 to 5.47 mM in the presence of 500 µg.mL-1 fractionated extract. Metabolite identification with LC-MS/MS QTOF suggested that galangin, kaempferol, and quercetin were responsible for the observed lipase inhibition.

Pancreatic lipase inhibition of strictinin isolated from Pu’er tea (Cammelia sinensis) and its anti-obesity effects in C57BL6 mice

2018 ◽  
Vol 48 ◽  
pp. 1-8 ◽  
Author(s):  
Tzu-Yin Chen ◽  
Miki M.C. Wang ◽  
Sheng-Kuo Hsieh ◽  
Meng-Huang Hsieh ◽  
Wen-Ying Chen ◽  
...  
Keyword(s):  
Pancreatic Lipase ◽  
Pancreatic Lipase Inhibition ◽  
Lipase Inhibition
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