scholarly journals Resistin in Human Seminal Plasma: Relationship with Lipid Peroxidation, CAT Activity, GSH/GSSG Ratio, and Semen Parameters

2019 ◽  
Vol 2019 ◽  
pp. 1-8
Author(s):  
Elena Moretti ◽  
Lucia Micheli ◽  
Daria Noto ◽  
Anna Ida Fiaschi ◽  
Andrea Menchiari ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Seminal Plasma ◽  
Sperm Quality ◽  
Semen Analysis ◽  
Group Versus ◽  
Annexin V ◽  
Semen Parameters ◽  
Human Seminal Plasma ◽  
Apoptosis And Necrosis ◽  
Infertile Patients

Resistin is an adipokine involved in inflammation and able to induce the expression of other proinflammatory cytokines. It is known that, in human semen, resistin is correlated with inflammatory cytokines and sperm quality. The aim of this prospective study was to explore the potential relationship between resistin, lipid peroxidation (LPO), catalase (CAT) activity, and reduced and oxidized glutathione (GSH/GSSG) ratio in semen samples of infertile patients with leukocytospermia (no. 19), infertile patients with varicocele (no. 17), and fertile men (no. 17). Semen analysis was performed following the WHO guidelines, and sperm apoptosis and necrosis were evaluated with annexin V/propidium iodide assay. Seminal plasma samples were used to determine resistin levels by an immunological method, MDA concentration by a HPLC analysis with UV detection, GSH/GSSG ratio by an enzymatic method, CAT activity by a spectrophotometric method. The results showed that, in both groups of infertile patients, semen parameters were significantly reduced (P<0.001) and sperm apoptosis and necrosis percentages were increased. Resistin levels were significantly higher in leukocytospermia and varicocele groups (P<0.001 and P<0.01, respectively) as well as MDA concentration (P<0.001) compared to controls. The MDA level was also significantly increased in the leukocytospermia group versus the varicocele group (P<0.05). The GSH/GSSG ratio was higher in fertile controls than the leukocytospermia group (P<0.05) and the varicocele group (P<0.001) and in the leukocytospermia group versus the varicocele group (P<0.05). Both the leukocytospermia and varicocele groups showed increased values of CAT activities (P<0.001) than controls. Briefly, the correlation between variables, calculated in the whole patient population, showed that resistin levels positively correlated with MDA levels, CAT activity, sperm apoptosis, and necrosis and negatively with sperm parameters and GSH/GSSG ratio. These results support an active role of resistin in an inflammatory process causing LPO, increase of CAT activity, and decrease of GSH/GSSG ratio in seminal plasma of infertile men vs. fertile controls.

scholarly journals Relationships between Ghrelin and Obestatin with MDA, Proinflammatory Cytokines, GSH/GSSG Ratio, Catalase Activity, and Semen Parameters in Infertile Patients with Leukocytospermia and Varicocele

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Lucia Micheli ◽  
Giulia Collodel ◽  
Daniela Cerretani ◽  
Andrea Menchiari ◽  
Daria Noto ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Reduced Glutathione ◽  
Semen Analysis ◽  
Antioxidant Properties ◽  
Annexin V ◽  
Protective Role ◽  
Semen Parameters ◽  
Biological Functions ◽  
Infertile Patients ◽  
Necrosis Factor

Ghrelin and obestatin are involved in many biological functions including reproduction. Growing evidences suggest that both peptides could exert protective and antioxidant activities. In this study, the relationships between ghrelin/obestatin, interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), malondialdehyde (MDA), reduced glutathione (GSH), oxidized glutathione (GSSG), expressed as the GSH/GSSG ratio, catalase (CAT), and semen parameters in infertile patients with varicocele or leukocytospermia and controls were investigated. Fifty-six infertile patients (32 with leukocytospermia and 24 with varicocele) and 14 controls participated in this study. Semen analysis was performed following the WHO guidelines. Apoptotic and necrotic sperm were scored by annexin V/propidium iodide assay. Seminal plasma samples were used for the following determinations: ghrelin, obestatin, IL-6, and TNF-α were measured by an immunological method, GSH/GSSG by an enzymatic method, and CAT by spectrophotometric determination. With respect to controls, both the leukocytospermia and varicocele groups showed altered sperm parameters, significantly increased sperm apoptosis (P=0.009 and P=0.011, respectively), IL-6 (P=0.0001 and P=0.004, respectively), and TNF-α levels (P=0.0001 and P=0.002, respectively); both groups had significantly decreased levels of ghrelin P=0.0001, obestatin (P=0.0001 and P=0.006, respectively), and GSH/GSSG ratio (P=0.003 and P=0.0001, respectively). The MDA concentration was significantly increased in the leukocytospermia group vs. controls P=0.0001, in the varicocele group vs. controls P=0.011, and in the leukocytospermia group vs. the varicocele group P=0.008. CAT activity was augmented in both the leukocytospermia and varicocele groups P=0.0001vs. controls. The results indicate that both ghrelin and obestatin may play a protective role in human semen and this effect is probably due to their antioxidant properties.

scholarly journals Impact of Seminal Plasma Trace Elements on Human Sperm Motility Parameters

2018 ◽  
Vol 56 (1) ◽  
pp. 15-20 ◽  
Author(s):  
Mohammad Mostakhdem Hashemi ◽  
Nasser Behnampour ◽  
Mojgan Nejabat ◽  
Afsaneh Tabandeh ◽  
Behrouz Ghazi-Moghaddam ◽  
...  
Keyword(s):  
Trace Elements ◽  
Sperm Motility ◽  
Seminal Plasma ◽  
Sperm Quality ◽  
Semen Analysis ◽  
Sperm Concentration ◽  
Negative Relationship ◽  
Human Sperm ◽  
Computer Assisted ◽  
Human Seminal Plasma

Abstract Introduction. Human seminal plasma contains a variety of macro and trace elements including magnesium (Mg), copper (Cu), zinc (Zn), and iron (Fe) that have essential roles in normal functioning of semen and its quality. The imbalance of these elements has been reported in several pathologic and male infertility disorders. Therefore, this study aimed to determine the levels of these elements in seminal plasma samples, their relationships with each other and their impact on sperm motility. Methods. Overall, 192 males (96 normospermic and 96 asthenospermic males) were enrolled in the study. Semen samples were collected by masturbation and computer-assisted/aided semen analysis of sperm motility was performed. The samples were centrifuged and seminal levels of Mg, Cu, Zn and Fe were measured using atomic absorption spectroscopy. Results. The levels of Zn did not differ between the two groups, while the levels of Mg, Cu, and Fe were significantly higher in normospermic males. Fe showed a positive correlation with Mg and Cu in asthenospermic group. However, a negative relationship was found between Mg and Fe levels and between Mg and sperm concentration in the normospermic group. Fe levels were higher in the normospermic group compared to the asthenospermic group. Nevertheless, increased Fe levels caused a decrease in most of sperm motility fractions. Conclusion: Elements play major roles in male fertility and directly affect sperm quality. According to the results of this study, the levels of Zn do not affect the sperm quality and motility, while Fe, Cu and Mg are decreased in males with sperm motility problems. Nevertheless, Fe levels can adversely affect sperm motility in normospermic men.

scholarly journals Humanin levels in human seminal plasma and spermatozoa are related to sperm quality

Andrology ◽  
2019 ◽  
Vol 7 (6) ◽  
pp. 859-866 ◽  
Author(s):  
M. Rao ◽  
Z. Wu ◽  
Y. Wen ◽  
R. Wang ◽  
S. Zhao ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Sperm Quality ◽  
Human Seminal Plasma

Trace Elements and Lipid Peroxidation in Human Seminal Plasma

2000 ◽  
Vol 76 (3) ◽  
pp. 207-216 ◽  
Author(s):  
Yeou-Lih Huang ◽  
Wei-Chang Tseng ◽  
Su-Ya Cheng ◽  
Te-Hsien Lin
Keyword(s):  
Lipid Peroxidation ◽  
Trace Elements ◽  
Seminal Plasma ◽  
Human Seminal Plasma

Characterization of Nuclease Activity in Human Seminal Plasma and its Relationship to Semen Parameters, Sperm DNA Fragmentation and Male Infertility

2016 ◽  
Vol 195 (1) ◽  
pp. 213-219 ◽  
Author(s):  
Alba Fernandez-Encinas ◽  
Agustí García-Peiró ◽  
Jordi Ribas-Maynou ◽  
Carlos Abad ◽  
María José Amengual ◽  
...  
Keyword(s):  
Male Infertility ◽  
Dna Fragmentation ◽  
Seminal Plasma ◽  
Nuclease Activity ◽  
Semen Parameters ◽  
Sperm Dna Fragmentation ◽  
Human Seminal Plasma ◽  
Sperm Dna

scholarly journals Decreased piRNAs in Infertile Semen Are Related to Downregulation of Sperm MitoPLD Expression

2021 ◽  
Vol 12 ◽  
Author(s):  
Yeting Hong ◽  
Yanqian Wu ◽  
Jianbin Zhang ◽  
Chong Yu ◽  
Lu Shen ◽  
...  
Keyword(s):  
Male Infertility ◽  
Sperm Motility ◽  
Seminal Plasma ◽  
Molecular Mechanisms ◽  
Molecular Biomarkers ◽  
Loss Of Function ◽  
Human Seminal Plasma ◽  
High Concentrations ◽  
Tight Correlation ◽  
Infertile Patients

Currently, the molecular mechanisms underlining male infertility are still poorly understood. Our previous study has demonstrated that PIWI-interacting RNAs (piRNAs) are downregulated in seminal plasma of infertile patients and can serve as molecular biomarkers for male infertility. However, the source and mechanism for the dysregulation of piRNAs remain obscure. In this study, we found that exosomes are present in high concentrations in human seminal plasma and confirmed that piRNAs are predominantly present in the exosomal fraction of seminal plasma. Moreover, we showed that piRNAs were significantly decreased in exosomes of asthenozoospermia patients compared with normozoospermic men. By systematically screening piRNA profiles in sperms of normozoospermic men and asthenozoospermia patients, we found that piRNAs were parallelly reduced during infertility. At last, we investigated the expression of some proteins that are essential for piRNAs biogenesis in sperms and therefore identified a tight correlation between the levels of spermatozoa piRNA and MitoPLD protein, suggesting that the loss-of-function of MitoPLD could cause a severe defect of piRNA accumulation in sperms. In summary, this study identified a parallel reduction of piRNAs and MitoPLD protein in sperms of asthenozoospermia patients, which may provide pathophysiological clues about sperm motility.

scholarly journals Cigarette smoking is associated with high level of ferroptosis in seminal plasma and affects semen quality

2020 ◽  
Author(s):  
Zhanhui Ou ◽  
Qirong Wen ◽  
Yu Deng ◽  
Yang Yu ◽  
Zhiheng Chen ◽  
...  
Keyword(s):  
Cigarette Smoking ◽  
Seminal Plasma ◽  
Molecular Mechanisms ◽  
Semen Quality ◽  
Semen Parameters ◽  
Oxygen Species ◽  
Cigarette Smoke Condensate ◽  
Human Seminal Plasma ◽  
High Level ◽  
Heavy Smokers

Abstract Purpose The effects of cigarette smoking on male semen quality are controversial, and the molecular mechanisms underlying how cigarette smoking affects semen quality are not clear yet. Methods In this study, semen samples from 70 heavy smokers and 75 non-smokers receiving infertility treatment were included. Basic semen parameters in non-smokers and heavy smokers were evaluated. Levels of glutathione (GSH), lipid reactive oxygen species (ROS), iron and GSH-dependent peroxidase 4 (GPX4) protein level) were observed in human seminal plasma and in GC-2Spd cells exposed to cigarette smoke condensate (CSC). Results Heavy smokers had significantly higher abnormalities (sperm viability and sperm progressive motility) than non-smoking counterparts. Comparing non-smokers group, GSH level was reduced in the group of heavy smokers (P<0.05). However, the level of lipid ROS and iron were significantly increased (P<0.05). Besides, GSH level was reduced following treatment with CSC for 24 h, while lipid ROS and iron levels were increased (P<0.05). However, the levels were reduced after being co-cultured with Ferrostatin-1 (Fer-1) (P<0.05). The level of GPX4 protein was reduced after being treated with CSC in 24 h, and increased after being co-cultured with Fer-1(P<0.05). Conclusion Cigarette smoking is associated with high level of ferroptosis in seminal plasma and affect semen quality.

Spermatic multinucleation in infertile agriculture workers exposed to carbofuranyl pesticide

1996 ◽  
Vol 54 ◽  
pp. 776-777
Author(s):  
G. Gallegos de L. ◽  
M.M. Arizpe S. ◽  
L.E. Alvarado C. ◽  
E. Ramirez B.
Keyword(s):  
Male Infertility ◽  
Causal Relationship ◽  
Sperm Motility ◽  
Detrimental Effect ◽  
Sperm Quality ◽  
Semen Analysis ◽  
Chemical Agents ◽  
And Function ◽  
Infertile Patients

It is known that the exposure to gonadotoxic agents cause male infertility in agricutural workers. Pesticides provoke a detrimental effect on the spermatogenesis characterized by low count of sprematozoa, as well as sperm motility and morphology alterations. Simultaneosly an increase in FSH seric levels is present.The semen analysis was proposed in 1975 to detect sperm quality and function alteration induced by chemical agents. There are many morphologic anomalies related to the noxious action of some gonadotoxic agents, ie. head deformities, microcephalia, tail defects, etc.We detected in our laboratory a pair of cases of infertile patients, that presented high levels of teratozoospermia with a high percentage of multinucleated spermatozoa and spermatids, and referred exposure to Carbofuranyl (Furadan 300). Since this agent has not been classified as gonadotoxic, we tried to show it's posible causal relationship with spermatic multinucleation phenomenon observed and the fertility of these patients.

scholarly journals The Effect of Human Chorionic Gonadotropin Therapy on Semen Parameters and Pregnancy Rate after Varicocelectomy

2017 ◽  
Vol 11 (2) ◽  
pp. 92-96
Author(s):  
Javanmard Babak ◽  
Fadavi Behruz ◽  
Yousefi Mohammadreza ◽  
Fallah-Karkan Morteza
Keyword(s):  
Pregnancy Rate ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Semen Analysis ◽  
Semen Parameters ◽  
Infertile Men ◽  
Hcg Therapy ◽  
Infertile Patients ◽  
Gonadotropin Therapy

Introduction: To study the stimulating effect of human chorionic gonadotropin (hCG) on spermatogenesis in patients with varicocele and infertility undergoing varicocelectomy. Materials and Methods: In the study, 188 infertile patients with varicocele were included. Open inguinal varicocelectomy was performed. They were randomized into 2 groups and hCG (91 patients) was administered intramuscularly by dosage of 5,000 international units every week for 3 months. A semen analysis was obtained at 6 months, post-operatively and cases were followed for 2 years for pregnancy report. Results: Semen analysis of the patients shows a significant improvement in all parameters 6 months after varicocelectomy without any superiority between the 2 groups. During the follow-up, 56 couples (61.5%) in hCG treated and 22 couples (22.7%) in the group treated only by varicocelectomy achieved pregnancy. Patients treated with varicocelectomy plus hCG therapy had a significant superior pregnancy rate compared to the other group (P=0.0001). Conclusion: Administration of hCG in this group of infertile patients might be helpful in order to enhance pregnancy rate. However some more conclusive studies are needed to be able to recommend such therapy for infertile men due to varicocele.

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