scholarly journals Automated and Reproducible Detection of Vascular Endothelial Growth Factor (VEGF) in Renal Tissue Sections

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Nayana Damiani Macedo ◽  
Aline Rodrigues Buzin ◽  
Isabela Bastos de Araujo ◽  
Breno Valentim Nogueira ◽  
Tadeu Uggere Andrade ◽  
...  
Keyword(s):  
Machine Learning ◽  
Vascular Endothelial Growth Factor ◽  
Image Analysis ◽  
Growth Factor ◽  
Kidney Tissue ◽  
High Sensitivity ◽  
Renal Tissue ◽  
Vascular Endothelial ◽  
Tissue Sections ◽  
Endothelial Growth Factor

Background. Manual analysis of tissue sections, such as for pathological diagnosis, requires an analyst with substantial knowledge and experience. Reproducible image analysis of biological samples is steadily gaining scientific importance. The aim of the present study was to employ image analysis followed by machine learning to identify vascular endothelial growth factor (VEGF) in kidney tissue that had been subjected to hypoxia. Methods. Light microscopy images of renal tissue sections stained for VEGF were analyzed. Subsequently, machine learning classified the cells as VEGF+ and VEGF- cells. Results. VEGF was detected and cells were counted with high sensitivity and specificity. Conclusion. With great clinical, diagnostic, and research potential, automatic image analysis offers a new quantitative capability, thereby adding numerical information to a mostly qualitative diagnostic approach.

Cyclosporine Toxicity and Vascular Endothelial Growth Factor (VEGF).

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 3924-3924
Author(s):  
Peter Kubisz ◽  
Barbara Grandtnerova ◽  
Ludovit Laca ◽  
Jan Stasko
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Graft Function ◽  
Renal Tissue ◽  
Gingival Hyperplasia ◽  
Kidney Graft ◽  
Vascular Endothelial ◽  
Vegf Mrna ◽  
Healthy Control ◽  
Endothelial Growth Factor

Abstract Vascular endothelial growth factor (VEGF) is a potent angiogenic and endothelial cell-specific mitogen. Expression of VEGF mRNA is increased under hypoxia/ischemia conditions. Its role in the setting of clinical kidney transplantation (Tx) is currently under investigation. Majority of works are dealing with gene polymorphisms and/or renal tissue expression. The aim of the study was to evaluate serum VEGF concentrations after kidney Tx and its relationship to graft outcome. Thirty-five adult patients (17 male, 18 female) treated with cyclosporine A or tacrolimus, at least 1 month after Tx and 22 healthy control (17 male, 5 female). Patients with acute rejection or infection were excluded. Serum VEGF (S-VEGF) concentrations were measured by Quantikine Immunoassay, RD Systems. S-VEGF concentrations were significantly higher after Tx than in healthy control (556 ± 463 pg/ml, vs 145 ± 74 pg/ml, p< 0.0001). No correlation was found with age, gender, time after Tx or type of calcineurin inhibitor. However, a significant correlation was found between basaline S-VEGF and S-creatinine (p< 0.05), S-VEGF and S-creatinine six months after Tx (p< 0.01) and between S-VEGF and cyclosporine toxicity defined as a gingival hyperplasia or biopsy proven nephrotoxicity (202 ± 121 pg/ml in stable patients vs 741 ± 436 pg/ml in cyclosporine toxicity group, p< 0.001). During six months of follow up, the kidney graft function was stable in 100% of patients with S-VEGF concentration ≤ 145 pg/ml but only in 50% of patients with S-VEGF concentration > 145 pg/ml (p < 0,05). We conclude that the S-VEGF concentration seems to be a marker of cyclosporine toxicity and prospective graft function deterioration. S-VEGF could be helpful to select patients who would have benefit from an early switch of immunosuppression.

scholarly journals Fabrication of Zinc Protoporphyrin-Modified Gold Electrode for Sensitive and Fast Detection of Vascular Endothelial Growth Factor

Chemosensors ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 21
Author(s):  
Hung-Yu Lin ◽  
Chin-Cheng Liao ◽  
Mu-Yi Hua
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Gold Electrode ◽  
Near Infrared ◽  
High Sensitivity ◽  
Zinc Protoporphyrin ◽  
Electrochemical Characteristics ◽  
Vascular Endothelial ◽  
Detection Range ◽  
Endothelial Growth Factor

Vascular endothelial growth factor (VEGF) is directly related to cancer growth and its distant spread, and thus, it is considered a promising biomarker for diagnosis and post-treatment monitoring of patients with malignancies. Zinc protoporphyrin (ZnPP) is a zinc-centered raw purple compound (protoporphyrin) that has unique optical and electrochemical characteristics. In this study, we used a ZnPP-modified gold electrode to generate a chemical bond with Avastin by self-assembly and fabricate a Au/ZnPP/Avastin electrode. Bovine serum protein (BSA) was added to the electrode to prevent non-specific linkage with biomolecules. The prepared Au/ZnPP/Avastin/BSA electrodes were used for the detection of VEGF by cyclic voltammetry and amperometry. The optical properties of ZnPP were analyzed with an ultraviolet/visible/near-infrared spectrometer and a photoluminescence spectrometer. The structural and hydrophilic/hydrophobic properties of the ZnPP-modified gold electrodes were investigated by Fourier-transform infrared spectroscopy and contact angle gauge, respectively. VEGF was detected with the Au/ZnPP/Avastin/BSA electrodes prepared either with (w/LT) or without light treatment (w/o LT). The w/LT electrode showed a linear range and a sensitivity of 0.1 pg/mL–10 ng/mL and 6.52 μA/log(pg/mL)-cm2, respectively; the corresponding values for the w/o LT electrode were 10 pg/mL–10 ng/mL and 3.15 μA/log(pg/mL)-cm2, respectively. The w/LT electrode had good specificity for VEGF and was minimally influenced by other molecules. The excellent detection range, high sensitivity, and high selectivity for VEGF detection indicate that Au/ZnPP/Avastin electrodes have great potential for diagnostic and prognostic applications in patients with malignancies.

A highly sensitive electrochemical aptasensor for vascular endothelial growth factor detection based on toehold-mediated strand displacement reaction

2021 ◽  
Author(s):  
Zhong Gao ◽  
Fangling Ren ◽  
Guangyi Yang ◽  
Guangjun Feng ◽  
Lun Wu ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
High Sensitivity ◽  
Vascular Endothelial ◽  
Electrochemical Aptasensor ◽  
Strand Displacement ◽  
Highly Sensitive ◽  
Recycling Amplification ◽  
Sensitivity Specificity ◽  
Endothelial Growth Factor

An electrochemical aptasensor with high sensitivity, specificity and good intra-day reproducibility is reported to meet the detection needs of vascular endothelial growth factor (VEGF). The toehold-mediated strand displacement recycling amplification...

Vascular endothelial growth factor enhances venous thrombus recanalisation and organisation

2003 ◽  
Vol 89 (01) ◽  
pp. 169-176 ◽  
Author(s):  
Matthew Waltham ◽  
Kevin Burnand ◽  
Michael Collins ◽  
Catharine McGuinness ◽  
Inderjit Singh ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Image Analysis ◽  
Growth Factor ◽  
Inferior Vena ◽  
Vena Cava ◽  
Vascular Endothelial ◽  
Control Solution ◽  
Pathological Angiogenesis ◽  
Endothelial Growth Factor ◽  
Vegf Protein

SummaryVascular endothelial growth factor (VEGF) is a regulator of physiological and pathological angiogenesis and is found in naturally resolving experimental venous thrombi, where it may also regulate recanalisation. In this study VEGF protein was injected into venous thrombi to determine if this enhanced recanalisation and organisation. A rat model of inferior vena cava (IVC) thrombosis was used. Thrombi were formed in 3 groups (n = 3 per group). 10 μl 125I-VEGF was directly injected into thrombus thirty minutes after induction. Three hours, 1 day and 6 days later thrombus, IVC, and other tissues were harvested. 125I-VEGF was mostly distributed in the thrombus and the IVC, with smaller amounts in other tissues. Thrombi were formed in a further 4 groups (n = 6 per group).Thirty minutes after induction control solution or 1 ng, 10 ng or 100 ng recombinant human VEGF165 was injected directly into the thrombus. Lumen recanalisation, thrombus organisation and monocyte content were measured on digitised sections by image analysis. In animals treated with 10 ng of VEGF there was a greater area of lumen recanalisation [mean 5492 pixels, standard error of mean (sem) 922] compared to controls (mean 2974, sem 385) (P = 0.005). There was a significant increase in the organisation score in all treated animals (1 ng: mean 70, sem 1.7, P = 0.0025; 10 ng: mean 70, sem 2.0, P = 0.0042; 100 ng: mean 72, sem 1.9, P = 0.0003) compared to controls (mean 63, sem 1.7). The monocyte content was lower in the animals treated with 1 ng VEGF (mean 3.8% of thrombus area, sem 0.3%) compared to controls (mean 5.5%, sem 0.4%) (P = 0.0008).The proportion of monocytes migrating to the centre of the thrombus increased in a dose-related manner. VEGF may prove to be of use in the treatment of venous thrombosis.

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