scholarly journals Oxidized Oils and Oxidized Proteins Induce Apoptosis in Granulosa Cells by Increasing Oxidative Stress in Ovaries of Laying Hens

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Ling Zhou ◽  
Xuemei Ding ◽  
Jianping Wang ◽  
Shiping Bai ◽  
Qiufeng Zeng ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Granulosa Cell ◽  
Granulosa Cells ◽  
Cell Apoptosis ◽  
Egg Production ◽  
Laying Hens ◽  
Corn Oil ◽  
Animal Feed ◽  
Corn Gluten Meal ◽  
Corn Gluten

The storage and preparation of corn for animal feed inevitably lead to lipid and protein peroxidation. Granulosa cells play an important role in follicular development in the ovaries, and hen laying productivity is likely to be dependent on follicle health and number. We hypothesized that oxidized oil and protein induce apoptosis via oxidative stress in laying hen granulosa cells. A sample of 360 38-week-old Lohmann commercial laying hens was used in a 2×2 factorial design for 8 weeks. Dietary treatments included dietary oil (fresh corn oil (FO) or oxidized corn oil (OO)) and corn gluten meal (fresh corn gluten meal (FP) or oxidized corn gluten meal (OP)). Productivity, ovarian histology, granulosa cell apoptosis, and indicators of oxidative stress were evaluated in all groups. Both dietary OO and OP decreased egg production and the average daily feed intake (ADFI) of laying hens. Flow cytometry, TUNEL, and real-time PCR revealed that both dietary OO and OP induced granulosa cell apoptosis in prehierarchical and hierarchical follicles. Furthermore, dietary OO and OP caused oxidative stress in prehierarchical and hierarchical follicles, as indicated by the downregulation of antioxidant-related-gene expression. Moreover, forkhead box O1 (FoxO1), extracellular regulated protein kinase (ERK), and c-Jun NH2 kinase (JNK) are involved in potential apoptosis regulation pathways in the granulosa cells of laying hens fed OO and OP, as indicated by the upregulation of FoxO1 expression and downregulation of ERK/JNK expression. These results indicate that OO and OP induce granulosa cell apoptosis via oxidative stress, and the combined use of OO and OP aggravates the adverse effects of oxidative stress in laying hens.

Malathion-Induced Granulosa Cell Apoptosis in Caprine Antral Follicles: An Ultrastructural and Flow Cytometric Analysis

2014 ◽  
Vol 20 (6) ◽  
pp. 1861-1868 ◽  
Author(s):  
Jitender K. Bhardwaj ◽  
Priyanka Saraf
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Granulosa Cell ◽  
Granulosa Cells ◽  
Cell Apoptosis ◽  
Ovarian Function ◽  
Flow Cytometric Analysis ◽  
Thiobarbituric Acid ◽  
Antral Follicles ◽  
Tbars Assay

AbstractOrganophosphate pesticides (OPs) like malathion interfere with normal ovarian function resulting in an increased incidence of atresia and granulosa cell apoptosis that plays a consequential role in the loss of ovarian follicles or follicular atresia. The aim of present study was to assess malathion-induced (100 nM) reproductive stress, ultrastructural damage and changes in apoptosis frequency in ovarian granulosa cells of antral follicles. Transmission electron microscopy (TEM) was employed for ultrastructural characterization, oxidative stress was evaluated using thiobarbituric acid reactive substances (TBARS) assay to measure lipid peroxidation, and apoptosis was quantified via flow cytometry. By TEM, apoptosis was identified by the presence of an indented nuclear membrane with blebbing, pyknotic crescent-shaped fragmented nuclei, increased vacuolization, degenerating mitochondria, and lipid droplets. The results indicate a significant increase in malondialdehyde (MDA) level (nmols/g wet tissue) at a 100 nM dose of malathion i.e. 7.57±0.033*, 8.53±0.12*, and 12.87±0.78** at 4, 6, or 8 h, respectively, as compared with controls (6.07±0.033, p<0.01*, p<0.05**) showing a positive correlation between malathion-induced lipid peroxidation and percentage of granulosa cell apoptosis (r=1; p<0.01). The parallel use of these three methods enabled us to determine the role of malathion in inducing apoptosis as a consequence of cytogenetic damage and oxidative stress generated in granulosa cells of antral follicles.

Morroniside suppresses hydrogen peroxide-stimulated autophagy and apoptosis in rat ovarian granulosa cells through the PI3K/AKT/mTOR pathway

2020 ◽  
pp. 096032712096076
Author(s):  
D Deng ◽  
J Yan ◽  
Y Wu ◽  
K Wu ◽  
W Li
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Cell Survival ◽  
Granulosa Cell ◽  
Granulosa Cells ◽  
Cell Apoptosis ◽  
Critical Role ◽  
Mtor Pathway ◽  
Dependent Manner ◽  
Ovarian Granulosa Cells

Previous evidences have indicated that granulosa cells play a critical role in follicular growth. Hydrogen peroxide (H2O2)-induced oxidative stress has been associated with ovarian granulosa cell apoptosis and ovarian function. Recently, a study highlighted the protective role of morroniside against H2O2-induced damage. In this study, we aimed to investigate the effects of morroniside on H2O2-stimulated rat ovarian granulosa cells and its underlying molecular mechanisms. Our results showed that H2O2 treatment suppressed cell survival and increased apoptosis in rat granulosa cells, while treatment with morroniside markedly increased H2O2-induced granulosa cell survival in a dose-dependent manner (0, 10, 50 and 100 µM). Moreover, treatment with 50 µM morroniside impeded H2O2-induced cell apoptosis. An elevation in intracellular ROS, MDA, SOD, GSH-Px, and CAT level was observed in H2O2-induced granulosa cells; however, this effect was abrogated by morroniside treatment. Further studies suggested that administration of morroniside inhibited H2O2-induced granulosa cell apoptosis and caspase-3 activity. In addition, after morroniside treatment of H2O2-stimulated granulosa cells, autophagy-related protein (LC3-II/LC3-I ratio) and beclin-1 expression was decreased and p62 level was increased. Interestingly, we found that morroniside treatment activated the PI3K/AKT/mTOR pathway in H2O2-stimulated granulosa cells. Finally, we showed that treatment with PI3K and mTOR inhibitors reversed the protective effects of morroniside on H2O2-induced granulosa cells. Taken together, our data suggest that treatment with morroniside decreased apoptosis, autophagy, and oxidative stress in rat granulosa cells through the PI3K/AKT/mTOR pathway.

Ufmylation regulates granulosa cell apoptosis via ER stress but not oxidative stress during goat follicular atresia

2021 ◽  
Vol 169 ◽  
pp. 47-55
Author(s):  
Xinyan Zhang ◽  
Tong Yu ◽  
Xinyan Guo ◽  
Ruixue Zhang ◽  
Yanni Jia ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Er Stress ◽  
Granulosa Cell ◽  
Cell Apoptosis ◽  
Follicular Atresia

P–229 Oxidative stress and Metformin; an in-vitro study on serum and primary human granulosa cell cultures

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
F Alam ◽  
R Rehman ◽  
N Farooqui ◽  
F Jehan ◽  
S H Abidi
Keyword(s):  
Oxidative Stress ◽  
Sample Size ◽  
Granulosa Cell ◽  
Cell Cultures ◽  
Granulosa Cells ◽  
Metformin Treatment ◽  
Serum Samples ◽  
Human Granulosa Cells ◽  
Human Granulosa Cell ◽  
Infertile Patients

Abstract Study question What is the effect of administration of Metformin on the oxidative stress (OS) levels in serum and primary human granulosa cell cultures of infertile females? Summary answer Metformin suppresses oxidative stress in serum and human granulosa cells and increases the expression of SIRT1 in OS induced environment. What is known already Oxidative stress (OS) is a resultant of mitochondrial dysfunction when it either fails to fight against the oxidants or the expression of the antioxidants is not sufficient. Cellular damage including DNA damage is a common resultant of oxidative stress. OS effects the oocyte maturation and moreover, the cleavage phase in the early embryonic stage. The raised levels of OS makers are hypothesized to compromise the nuclear maturation and the mitotic spindles of the maturing oocytes. Metformin seemed to decrease oxidative stress and improve insulin resistance, dyslipidaemia and endothelial dysfunction in PCOS patients Study design, size, duration This cross-sectional study was conducted from August 2017 – July 2019, at Aga Khan Hospital in collaboration with Australian Concept Infertility Medical Centre (ACIMC) on ten infertile patients undergoing egg retrieval after ethical approval from of Aga Khan Hospital (AKU-ERC–2018–0557–601). Participants/materials, setting, methods Serum samples were obtained and analysed. Follicular fluid of these subjects was collected for establishment of primary cell culture model of normal human granulosa cells (hGCs). Serum and hGC cultures were grouped as; a) control: treatment, b) Test1: H2O2 induced OS, and c) Test2: H2O2 induced OS treated with metformin. OS was estimated in all groups by Mishra method. The two Test groups were assessed for SIRT1 levels using quantitative PCR employing SIRT1 specific primers Main results and the role of chance With mean age of 32.04 ± 2.29 years the mean BMI was 27.61 ± 2.15 kg/m2. OS was induced and measured by an increase in optical density (OD) in hGC Test samples which showed 0.28 (0.16–0.40) OD when compared with control hGC samples 0.153 (0.09–0.23). There was a significant reduction in ODs after metformin treatment in the stress induced cells 0.182 (0.05–0.30). A similar pattern was observed in the serum samples in ODs; control: 0.105 (0.09–0.15), stress induced samples: 0.199 (0.19–0.20). and stress induced serum sample with metformin treatment: 0.1415 (0.06–0.18). The Ct values obtained to express the effect of metformin on SIRT1 levels, for OS induced (Test1) and OS induced metformin treated (Test2) cells were found to be 29.12 and 26.42, respectively. We also observed a significant (85%) difference in the fold change of SIRT1 expression between metformin treated and untreated cells. Limitations, reasons for caution Small sample size is the limitation of this study. The impact of metformin on cell cultures due to different causes of infertility could not be ascertained Wider implications of the findings: Metformin suppresses oxidative stress in serum and human granulosa cells and increases the expression of SIRT1 in OS induced environment, therefore, metformin may be considered as a treatment of oxidative stress in infertile patients. Randomized control trial with large sample size is recommended to confirm the cause and effect relationship. Trial registration number Not applicable

scholarly journals Di-(2-ethylhexyl) phthalate (DEHP) inhibits steroidogenesis and induces mitochondria-ROS mediated apoptosis in rat ovarian granulosa cells

2019 ◽  
Vol 8 (3) ◽  
pp. 381-394 ◽  
Author(s):  
Anima Tripathi ◽  
Vivek Pandey ◽  
Alakh N. Sahu ◽  
Alok Singh ◽  
Pawan K. Dubey
Keyword(s):  
Oxidative Stress ◽  
Granulosa Cell ◽  
Granulosa Cells ◽  
Ovarian Granulosa Cells ◽  
Ethylhexyl Phthalate

Increased oxidative stress (OS) due to ubiquitous exposure to di-(2-ethylhexyl) phthalate (DEHP) can affect the quality of oocytes by inducing apoptosis and hampering granulosa cell mediated steroidogenesis.

scholarly journals Early postmolt performance of laying hens fed a low-protein corn molt diet supplemented with corn gluten meal, feather meal, methionine, and lysine

1999 ◽  
Vol 78 (8) ◽  
pp. 1132-1137 ◽  
Author(s):  
KW Koelkebeck ◽  
CM Parsons ◽  
RW Leeper ◽  
S Jin ◽  
MW Douglas
Keyword(s):  
Laying Hens ◽  
Feather Meal ◽  
Corn Gluten Meal ◽  
Low Protein ◽  
Corn Gluten

scholarly journals Involvement of the Up-regulated FoxO1 Expression in Follicular Granulosa Cell Apoptosis Induced by Oxidative Stress

2012 ◽  
Vol 287 (31) ◽  
pp. 25727-25740 ◽  
Author(s):  
Ming Shen ◽  
Fei Lin ◽  
Jiaqing Zhang ◽  
Yiting Tang ◽  
Wei-Kang Chen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Granulosa Cell ◽  
Cell Apoptosis

scholarly journals Role of the SIRT1/p53 regulatory axis in oxidative stress‑mediated granulosa cell apoptosis

2020 ◽  
Vol 23 (1) ◽  
pp. 1-1
Author(s):  
Sang-Ah Park ◽  
Na-Rae Joo ◽  
Jung-Hwan Park ◽  
Sang-Muk Oh
Keyword(s):  
Oxidative Stress ◽  
Granulosa Cell ◽  
Cell Apoptosis

scholarly journals Diversity of Feed Storage Pest Beetle in Banten Province

2018 ◽  
Vol 22 (1) ◽  
pp. 20
Author(s):  
Nasrul Friamsa ◽  
Witjaksono Witjaksono ◽  
Arman Wijonarko
Keyword(s):  
Tribolium Castaneum ◽  
Soybean Meal ◽  
Animal Feed ◽  
Raw Materials ◽  
Sitophilus Zeamais ◽  
Beetle Species ◽  
Cryptolestes Ferrugineus ◽  
Oryzaephilus Surinamensis ◽  
Corn Gluten Meal ◽  
Corn Gluten

Banten province is a growing industrial area, where many industrial items were prepared including animal feed whose raw materials are mostly imported from abroad. Therefore, monitoring feed storage is very important to ensure the existence of pest insects in storage warehouse and to prevent the entry of plant quarantine pest organism (OPTK) which may be carried by imported feed raw materials. The identification, diversity and evenness of pest beetle species in five feed storages in Banten province region have been done. Feed sampling was done using hand sampling method. Samples taken from the diagonal corner and center of storage, respectively as much as 250 grams four times within one-week interval. The results were that 13 species of pest beetles from seven families were intercepted. Cryptolestes ferrugineus, Rhizoperta dominica, and Tribolium castaneum pest beetles were the dominant insects attacking five storages. Specifically, the dominant pest beetles in each commodity were: T. castaneum on soybean meal (SBM); C. ferrugineus and T. castaneum on corn; T. castaneum and R. dominica on corn gluten meal (CGM); T. castaneum and Sitophilus zeamais on wheat; T. castaneum on soybeans; and Oryzaephilus surinamensis in sorghum. Environmental factors, the type and duration of stored commodities were found to be the factors supporting the existence of pest beetle species. The highest diversity of pest beetle varieties was found in warehouse A with a value of 1.552 which was considered as moderate diversity. The evenness index of pest beetle species showed that warehouses A and E were in unstable conditions. Meanwhile, the warehouses B, C, and D were in  depressed conditions dominated by certain species of pest beetle. IntisariProvinsi Banten merupakan daerah industri yang terus berkembang, termasuk industri pakan ternak yang bahan bakunya sebagian besar diimpor dari luar negeri. Oleh sebab itu, pengawasan terhadap gudang penyimpanan pakan sangat penting dilakukan untuk mengawasi keberadaan serangga hama pada gudang penyimpanan dan mencegah masuknya Organisme Pengganggu Tumbuhan Karantina (OPTK) yang mungkin terbawa bahan baku pakan yang diimpor. Identifikasi, keanekaragaman serta kemerataan jenis kumbang hama pada lima gudang penyimpanan pakan di wilayah Banten telah dilakukan. Pengambilan sampel bahan pakan menggunakan metode hand sampling. Sampel diambil pada bagian sudut dan bagian tengah secara diagonal masing-masing sebanyak 250 gram sebanyak 4 kali dengan interval waktu 1 minggu sekali. Hasil penelitian diperoleh 13 jenis kumbang hama dari 7 famili. Kumbang hama jenis Cryptolestes ferrugineus, Rhyzoperta dominica, dan Tribolium castaneum merupakan serangga yang dominan menyerang kelima gudang penyimpanan. Jenis kumbang hama yang dominan pada masing-masing komoditas adalah T. castaneum pada soybean meal (SBM); C. ferrugineus dan T. castaneum pada komoditas jagung; T. castaneum dan R. dominica pada corn gluten meal (CGM); T. castaneum dan Sitophilus zeamais pada gandum; T. castaneum pada kedelai; dan Oryzaephilus surinamensis pada sorgum. Faktor lingkungan, jenis dan lama komoditas yang disimpan merupakan faktor pendukung keberadaan suatu jenis kumbang hama. Indeks keanekaragaman jenis kumbang hama tertinggi ditemukan pada gudang A dengan nilai 1,552 menggambarkan keanekaragaman tergolong sedang. Nilai indeks kemerataan jenis kumbang hama menunjukkan bahwa gudang A dan gudang E termasuk dalam kondisi labil; sedangkan gudang B, C, dan D termasuk dalam kondisi tertekan dengan didominasi oleh jenis kumbang hama tertentu.

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