Electroacupuncture Ameliorates Acute Myocardial Ischemia: A Potential Role of the Locus Coeruleus

The locus coeruleus (LC) is closely linked with cardiovascular disease. However, whether it mediates the alleviating effect of electroacupuncture (EA) on acute myocardial ischemia (AMI) remains unclear. A rat model of myocardial ischemia was established through occlusion of the left anterior descending coronary artery. Multichannel in vivo recording and other techniques were used to assess neurons in the LC, norepinephrine (NE) and dopamine (DA) levels in central and myocardial tissue, serum levels of inflammatory factors, and cardiac function. After induction of AMI, LC neuron activity increased and the central NE concentrations increased, while those of DA decreased. Moreover, the serum levels of high-sensitivity C-reactive protein (hs-CRP) increased, whereas those of interleukin-10 (IL-10) decreased. However, these effects were reversed by EA. Additionally, LC lesioning affected NE and DA levels in myocardial tissue and weakened the antimyocardial ischemic effect of EA. Collectively, our results indicated that LC is closely related to AMI and plays an important role in the antimyocardial ischemic effect of EA. This mechanism may be related to inhibition of LC neuron activity by EA, which inhibits the release of large amounts of hs-CRP and promotes that of IL-10 in the serum. Besides, after LC lesioning, EA may improve cardiac function by inhibiting the release of large amounts of NE and promoting the release of DA in myocardial tissue.

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In Vivo MRI Visualization of Acute Myocardial Ischemia and Reperfusion in Ferrets by the Persistent Action of the Contrast Agent Gd(BME-DTTA)

Circulation ◽

10.1161/01.cir.92.12.3549 ◽

1995 ◽

Vol 92 (12) ◽

pp. 3549-3559 ◽

Cited By ~ 12

Author(s):

Tamás Simor ◽

Wen-Jang Chu ◽

Lynne Johnson ◽

Andras Safranko ◽

Mark Doyle ◽

...

Keyword(s):

Myocardial Ischemia ◽

Contrast Agent ◽

Acute Myocardial Ischemia ◽

Ischemia And Reperfusion ◽

Myocardial Ischemia And Reperfusion

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Study on the Effects of Kuanxiong Aerosol on the Isolated Artery and Rabbits Acute Myocardial Ischemia Model

Combinatorial Chemistry & High Throughput Screening ◽

10.2174/1386207324666210811142312 ◽

2021 ◽

Vol 24 ◽

Author(s):

Xia Liu ◽

Feihua Huang ◽

Xiao Lu ◽

Yuji Wang ◽

Tingting Cai ◽

...

Keyword(s):

Myocardial Ischemia ◽

Troponin T ◽

Pharmacological Study ◽

Acute Myocardial Ischemia ◽

Chinese Herbal Compound ◽

Long Acting ◽

Traditional Chinese Medicine Preparation ◽

T Waves

Background: Kuan xiong aerosol (KXA) is a kind of Chinese herbal compound used to regulating qi-flowing for relieving pain and improving angina. However, little pharmacological study of this traditional Chinese medicine preparation has been reported to confirm these activities. Objective: This article aims to observe the effect of resisting acute myocardial ischemia (AMI) in vivo and dilating vessel in vitro of KXA. Materials: The AMI model involves intravenously injecting pituitary (2 U.kg-1) into the ear of rabbits. Electrocardiograph (ECG) T waves were then recorded after administration and the falling range was calculated. Following this, the level of serum Cardiac troponin T (cTn-T) and the histopathology of the cardiac muscle tissue was evaluated. In vitro, the effect of KXA on vasodilation of isolated aortic rings that had been pre-contracted with KCl (30 mM) was observed. Results: It was found KXA reduced ECG ST-T waves and serum cTn-T in the rabbit AMI model, protected myocardial tissue from fracturing and loss of myocardial fibers, and inhibited inflammatory cell infiltration, cavitation degeneration and karyopyknosis of the myocardial matrix. Furthermore, the administration of 0.215, 1.075 and 2.150 mg.mL-1 KXA resulted in significant relaxation of the aortic rings at a rate of 69.63 %, 90.14 % and 118.72 % (p < 0.01) of the untreated ones, and a second shrinkage ratio of 20.17 %, 4.29 %, and 4.54 % (p < 0.01) of the untreated ones, respectively. Conclusions: these results suggest KXA protects against AMI, contributes to dilation of blood vessels and has long-acting effectiveness.

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Effect of adenosine on recovery of phosphorous metabolites in acute myocardial ischemia-reperfusion : In vivo P MR spectroscopic assessment in cats

Korean Circulation Journal ◽

10.4070/kcj.1993.23.2.207 ◽

1993 ◽

Vol 23 (2) ◽

pp. 207 ◽

Cited By ~ 1

Author(s):

Seong Wook Park ◽

Mi Young Kim ◽

Tae Hwan Lim ◽

Pyung Hwan Park ◽

Dong Man Seo ◽

...

Keyword(s):

Myocardial Ischemia ◽

Ischemia Reperfusion ◽

Acute Myocardial Ischemia ◽

Myocardial Ischemia Reperfusion

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LncRNA PVT1 Knockdown Ameliorates Myocardial Ischemia Reperfusion Damage via Suppressing Gasdermin D-Mediated Pyroptosis in Cardiomyocytes

Frontiers in Cardiovascular Medicine ◽

10.3389/fcvm.2021.747802 ◽

2021 ◽

Vol 8 ◽

Author(s):

Cuizhi Li ◽

Huafeng Song ◽

Chunlin Chen ◽

Shaoxian Chen ◽

Qiyu Zhang ◽

...

Keyword(s):

Myocardial Ischemia ◽

Cell Viability ◽

Ischemia Reperfusion ◽

Inflammatory Factors ◽

H9c2 Cells ◽

Myocardial Ischemia Reperfusion ◽

Tissue Specimens ◽

Masson Staining

Objective: Myocardial ischemia reperfusion (I/R) damage is a life-threatening vascular emergency after myocardial infarction. Here, we observed the cardioprotective effect of long non-coding RNA (lncRNA) PVT1 knockdown against myocardial I/R damage.Methods: This study constructed a myocardial I/R-induced mouse model and a hypoxia/reoxygenation (H/R)-treated H9C2 cells. PVT1 expression was examined via RT-qPCR. After silencing PVT1 via shRNA against PVT1, H&E, and Masson staining was performed to observe myocardial I/R damage. Indicators of myocardial injury including cTnI, LDH, BNP, and CK-MB were examined by ELISA. Inflammatory factors (TNF-α, IL-1β, and IL-6), Gasdermin D (GSDMD), and Caspase1 were detected via RT-qPCR, western blot, immunohistochemistry, or immunofluorescence. Furthermore, CCK-8 and flow cytometry were presented for detecting cell viability and apoptosis.Results: LncRNA PVT1 was markedly up-regulated in myocardial I/R tissue specimens as well as H/R-induced H9C2 cells. Silencing PVT1 significantly lowered serum levels of cTnI, LDH, BNP, and CK-MB in myocardial I/R mice. H&E and Masson staining showed that silencing PVT1 alleviated myocardial I/R injury. PVT1 knockdown significantly lowered the production and release of inflammatory factors as well as inhibited the expression of GSDMD-N and Caspase1 in myocardial I/R tissue specimens as well as H/R-induced H9C2 cells. Moreover, silencing PVT1 facilitated cell viability and induced apoptosis of H/R-treated H9C2 cells.Conclusion: Our findings demonstrated that silencing PVT1 could alleviate myocardial I/R damage through suppressing GSDMD-mediated pyroptosis in vivo and in vitro. Thus, PVT1 knockdown may offer an alternative therapeutic strategy against myocardial I/R damage.

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Reynoutrin Improves Ischemic Heart Failure in Rats Via Targeting S100A1

Frontiers in Pharmacology ◽

10.3389/fphar.2021.703962 ◽

2021 ◽

Vol 12 ◽

Author(s):

Wenkai Yang ◽

Hanjian Tu ◽

Kai Tang ◽

Haozhong Huang ◽

Shi Ou ◽

...

Keyword(s):

Oxidative Stress ◽

Heart Failure ◽

Cardiac Function ◽

Protein Expression ◽

Myocardial Fibrosis ◽

Ischemic Heart ◽

Inflammatory Factors ◽

Myocardial Tissue ◽

Ischemic Heart Failure ◽

Tgf Β1

This study investigated the effects of reynoutrin on the improvement of ischemic heart failure (IHF) and its possible mechanism in rats. The rat heart failure model was established by permanently ligating the left anterior descending coronary artery (LAD) and administering different doses of reynoutrin. Cardiac function, inflammatory factors releasing, oxidative stress, cardiomyocytes apoptosis, and myocardial fibrosis were evaluated. Western blotting was used to determine protein expression levels of S100 calcium-binding protein A1 (S100A1), matrix metallopeptidase 2(MMP2), MMP9, phosphorylated (p-) p65, and transforming growth factor -β1 (TGF-β1) in myocardial tissue of the left ventricle. Results showed that reynoutrin significantly improved cardiac function, suppressed the release of inflammatory factors, reduced oxidative stress, inhibited cardiomyocytes apoptosis, and attenuated myocardial fibrosis in rats with IHF. In rat myocardial tissue, permanent LAD-ligation resulted in a significant down-regulation in S100A1 expression, whereas reynoutrin significantly up-regulated S100A1 protein expression while down-regulating MMP2, MMP9, p-p65, and TGF-β1 expressions. However, when S100A1 was knocked down in myocardial tissue, the above-mentioned positive effects of reynoutrin were significantly reversed. Reynoutrin is a potential natural drug for the treatment of IHF, and its mechanism of action involves the up-regulation of S100A1 expression, thereby inhibiting expressions of MMPs and the transcriptional activity of nuclear factor kappa-B.

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Hypothermic retroperfusion treatment of acute myocardial ischemia: Effects on cardiac function and infarct size

The American Journal of Cardiology ◽

10.1016/0002-9149(81)90820-1 ◽

1981 ◽

Vol 47 ◽

pp. 436 ◽

Cited By ~ 4

Author(s):

Roberto Haendchen ◽

Noboru Aosaki ◽

Takahisa Uchiyama ◽

Xavier Escudero ◽

Jeffrey Broffman ◽

...

Keyword(s):

Myocardial Ischemia ◽

Cardiac Function ◽

Infarct Size ◽

Acute Myocardial Ischemia

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Metabolic alterations in acute myocardial ischemia-reperfusion injury and necrosis using in vivo hyperpolarized [1-13C] pyruvate MR spectroscopy

Scientific Reports ◽

10.1038/s41598-019-54965-7 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 3

Author(s):

Chung-Man Moon ◽

Yun-Hyeon Kim ◽

Young-Keun Ahn ◽

Myung-Ho Jeong ◽

Gwang-Woo Jeong

Keyword(s):

Myocardial Ischemia ◽

Reperfusion Injury ◽

Real Time ◽

Intracellular Ph ◽

Ischemia Reperfusion ◽

Acute Myocardial Ischemia ◽

Total Carbon ◽

Myocardial Ischemia Reperfusion ◽

Ph Level

AbstractThis study aimed to investigate real-time early detection of metabolic alteration in a rat model with acute myocardial ischemia-reperfusion (AMI/R) injury and myocardial necrosis, as well as its correlation with intracellular pH level using in vivo hyperpolarized [1-13C] pyruvate magnetic resonance spectroscopy (MRS). Hyperpolarized 13C MRS was performed on the myocardium of 8 sham-operated control rats and 8 rats with AMI/R injury, and 8 sham-operated control rats and 8 rats with AMI-induced necrosis. Also, the correlations of levels of [1-13C] metabolites with pH were analyzed by Spearman’s correlation test. The AMI/R and necrosis groups showed significantly higher ratios of [1-13C] lactate (Lac)/bicarbonate (Bicar) and [1-13C] Lac/total carbon (tC), and lower ratios of 13C Bicar/Lac + alanine (Ala), and 13C Bicar/tC than those of the sham-operated control group. Moreover, the necrosis group showed significantly higher ratios of [1-13C] Lac/Bicar and [1-13C] Lac/tC, and lower ratios of 13C Bicar/Lac + Ala and 13C Bicar/tC than those of the AMI/R group. These results were consistent with the pattern for in vivo the area under the curve (AUC) ratios. In addition, levels of [1-13C] Lac/Bicar and [1-13C] Lac/tC were negatively correlated with pH levels, whereas 13C Bicar/Lac + Ala and 13C Bicar/tC levels were positively correlated with pH levels. The levels of [1-13C] Lac and 13C Bicar will be helpful for non-invasively evaluating the early stage of AMI/R and necrosis in conjunction with reperfusion injury of the heart. These findings have potential application to real-time evaluation of cardiac malfunction accompanied by changes in intracellular pH level and enzymatic activity.

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Influence of acute microwave radiation on cardiac function in normal and myocardial ischemic cats

Journal of Applied Physiology ◽

10.1152/jappl.1981.50.5.931 ◽

1981 ◽

Vol 50 (5) ◽

pp. 931-935 ◽

Cited By ~ 4

Author(s):

M. J. Galvin ◽

D. I. McRee

Keyword(s):

Coronary Artery ◽

Myocardial Ischemia ◽

Microwave Irradiation ◽

Cardiac Function ◽

Microwave Radiation ◽

Cardiac Tissue ◽

Continuous Wave ◽

Arterial Blood

Exposure of biological specimens to microwave radiation in vivo and in vitro has been reported to cause alterations to the cardiovascular system. In addition, microwave radiation may cause effects in damaged cardiac tissue that are not observed in normal tissue. In this study, we examined the influence of direct microwave irradiation (2.45 GHz, continuous wave) of the intact exposed heart on cardiac function in cats with and without myocardial ischemia. Myocardial ischemia was induced by occlusion of the left anterior descending coronary artery. In the sham-nonexposed and sham-plus-microwave exposed animals the coronary artery was isolated but not occluded. The exposed hearts were either irradiated at a specific absorption rate (SAR) of 30 mW/g or not irradiated, and were monitored for 5 h. At a SAR of 30 mW/g, the temperature of the exposed tissue increased at an initial rate of 0.43 degrees C/min in dead cats. However, in live animals, no increases in aortic blood temperatures occurred during irradiation. Mean arterial blood pressure, cardiac output, heart rate, plasma and myocardial creatine phosphokinase, and S-T segment were not influenced by 5 h of microwave irradiation of the myocardium in cats with or without myocardial ischemia.

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