Clinical, Histological, and Scintigraphic Comparative Study of the Use of Mandibular Bone Marrow and Peripheral Blood in Bone Neoformation

Background and Purpose. To evaluate the capacity of mandibular bone marrow blood aspirate associated with biomaterials to stimulate bone tissue neoformation compared to the use of peripheral blood aspirate in patients with bone loss in the premaxillary region. Materials and Methods. The study included 16 patients with maxillary atresia. The region was grafted with xenograft blocks associated with the following treatments: G1, the patient’s peripheral blood during surgery, and G2, dripping of mandibular bone marrow blood until the xenograft was completely wet. After 7 and 14 days, scintigraphic images of the regions of interest (ROI) were taken to quantify pixels, which indicate osteogenic activity. Additionally, trephined samples obtained at the time of implant placement were stained in H&E, and newly formed bone tissue was quantified. The data were tabulated and statistically analyzed at a significance level of 5%. Results. Scintigraphic data showed greater osteogenic activity with mandibular bone marrow blood (G2) at all times evaluated p < 0.05 . As for the histomorphometric analysis, a greater amount of bone tissue was observed in samples treated with mandibular bone marrow blood (G2) compared to peripheral blood (G1) p < 0.05 . Conclusions. The appositional bone reconstruction technique in the block associated with mandibular bone marrow blood increased bone neoformation and osteogenic activity compared to conventional graft treatment with peripheral blood.

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Influence of high insertion torque on implant placement: an anisotropic bone stress analysis

Brazilian Dental Journal ◽

10.1590/s0103-64402010000600005 ◽

2010 ◽

Vol 21 (6) ◽

pp. 508-514 ◽

Cited By ~ 9

Author(s):

Bruno Salles Sotto-Maior ◽

Eduardo Passos Rocha ◽

Erika Oliveira de Almeida ◽

Amilcar Chagas Freitas-Júnior ◽

Rodolfo Bruniera Anchieta ◽

...

Keyword(s):

Bone Tissue ◽

Cancellous Bone ◽

Maximum Principal Stress ◽

Insertion Torque ◽

Stress Concentrations ◽

Bone Implant ◽

Significance Level ◽

Bone Stress ◽

Implant Placement ◽

Solid Works

The aim of this study was to evaluate the influence of the high values of insertion torques on the stress and strain distribution in cortical and cancellous bones. Based on tomography imaging, a representative mathematical model of a partial maxilla was built using Mimics 11.11 and Solid Works 2010 softwares. Six models were built and each of them received an implant with one of the following insertion torques: 30, 40, 50, 60, 70 or 80 Ncm on the external hexagon. The cortical and cancellous bones were considered anisotropic. The bone/implant interface was considered perfectly bonded. The numerical analysis was carried out using Ansys Workbench 10.0. The convergence of analysis (6%) drove the mesh refinement. Maximum principal stress (Ïƒmax) and maximum principal strain (Îµmax) were obtained for cortical and cancellous bones around to implant. Pearson's correlation test was used to determine the correlation between insertion torque and stress concentration in the periimplant bone tissue, considering the significance level at 5%. The increase in the insertion torque generated an increase in the Ïƒmax and Îµmax values for cortical and cancellous bone. The Ïƒmax was smaller for the cancellous bone, with greater stress variation among the insertion torques. The Îµmax was higher in the cancellous bone in comparison to the cortical bone. According to the methodology used and the limits of this study, it can be concluded that higher insertion torques increased tensile and compressive stress concentrations in the periimplant bone tissue.

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ARE THE MEGAKARYOCYTES OF THE SPLEEN OF WHITE LABORATORY RATS IDENTICAL TO MEGACARIOCYTES OF THE BONE MARROW?

MORPHOLOGICAL NEWSLETTER ◽

10.20340/mv-mn.17(25).03.69-73 ◽

2017 ◽

Vol 25 (3) ◽

pp. 69-73

Author(s):

E. V. Lyubaeva ◽

V. A. Vankov ◽

T. V. Moiseeva ◽

I. I. Markov

Keyword(s):

Bone Marrow ◽

Trabecular Bone ◽

Bone Tissue ◽

Morphological Data ◽

Paraffin Sections ◽

Mandibular Bone ◽

Laboratory Rats ◽

White Rats ◽

Hematoxylin And Eosin ◽

Trabecular Bone Tissue

Many aspects of spleen histophysiology in humans and mammals still remain unsolved until today. This includes the spleen in white rats. The presence of megakaryocytes in its parenchyma has been only briefly indicated in some papers. As for appropriate information, let alone illustration, it is not available even in manuals on histology or the current histological atlases. The aim of the study is to obtain comparative morphological data on the spleen and marrow megakaryocytes in white rats. The object of this study is the spleen and marrow of the mandibular bone of outbred white rats (n=17) weighing 180,0-200,0 g. Staining the spleen preparations and mandibular trabecular bone tissue paraffin sections with hematoxylin and eosin, Azur-II-eosin and according to Van Gieson. In the spleen of white rats, megakaryocytes they appear identical to the marrow megakaryocytes.

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Remifentanil Decreases Mandibular Bone Marrow Blood Flow During Propofol or Sevoflurane Anesthesia in Rabbits

Journal of Oral and Maxillofacial Surgery ◽

10.1016/j.joms.2008.06.008 ◽

2009 ◽

Vol 67 (6) ◽

pp. 1245-1250 ◽

Cited By ~ 23

Author(s):

Masahiro Kemmochi ◽

Tatsuya Ichinohe ◽

Yuzuru Kaneko

Keyword(s):

Bone Marrow ◽

Blood Flow ◽

Sevoflurane Anesthesia ◽

Mandibular Bone ◽

Bone Marrow Blood

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Differential analysis of peripheral blood- and bone marrow-derived endothelial progenitor cells for enhanced vascularization in bone tissue engineering

Journal of Orthopaedic Research® ◽

10.1002/jor.22097 ◽

2012 ◽

Vol 30 (9) ◽

pp. 1507-1515 ◽

Cited By ~ 53

Author(s):

Ami R. Amini ◽

Cato T. Laurencin ◽

Syam P. Nukavarapu

Keyword(s):

Tissue Engineering ◽

Bone Marrow ◽

Bone Tissue Engineering ◽

Progenitor Cells ◽

Bone Tissue ◽

Endothelial Progenitor Cells ◽

Peripheral Blood ◽

Differential Analysis ◽

Endothelial Progenitor

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Bone Marrow Contains High Levels of Microparticles

Blood ◽

10.1182/blood.v124.21.5145.5145 ◽

2014 ◽

Vol 124 (21) ◽

pp. 5145-5145

Author(s):

Andreas Rank ◽

Rienk Nieuwland ◽

Anton Koehler ◽

Hans Jochem Kolb ◽

Bettina Toth

Keyword(s):

Bone Marrow ◽

Endothelial Cells ◽

Red Blood Cells ◽

Progenitor Cells ◽

Peripheral Blood ◽

Blood Cells ◽

Conflicts Of Interest ◽

White Blood Cells ◽

Cell Source ◽

Bone Marrow Blood

Abstract Background: Microparticles (MP) are shed in humans from a broad variety of cells and play an important role in activation of coagulation, cell to cell interaction and transport of membrane components. Alternations in plasma levels have been found in a variety of diseases, mostly associated with thrombosis and inflammation. However, little is known about tissue levels of MP. The aim of this pilot study was to investigate the bone marrow compartment as a possible source of circulating MP. Material and Methods: MP were analysed in bone marrow blood and peripheral blood in 12 healthy stem cell donors. MPs were isolated and measured by flow-cytometric double-staining (FACScalibur, BD) with Anexin V and cell-specific antibodies for hematopoietic cells, platelets, white blood cells, red blood cells and endothelial cells. Statistical analysis was performed with SPSS for Windows 20.0. Results: Total MP levels differed between bone marrow and peripheral blood: 14.8 x109/l [8.5–19.3] vs. 9.2 x109/l [3.8–14.8] (median value [25-75 percentile]), p=0.060. Pattern of MP´s origin varied likewise: in bone marrow blood (bmb), main cell source of MP were CD235a positive red blood cells/erythropoetic cells (6.4x109/l [3.7–13.7], 43.2% of total bmb MP), CD61 positive platelets/megacaryocytes (4.1x109/l [1.7–10.1], 27.7% of total bmb MP), CD45 positive leucocytes/myeloic and lymphatic progenitor cells (3.8x109/l [2.7-4.9], 25.7% of total bmb MP) and CD62e positive endothelial cells (0.3x109/l [0.1-1.0], 2.0% of total bmb MP). In contrast, MP in peripheral blood (pb) mainly derived from CD61 positive platelets/megacaryocytes (8.5x109/l [2.4–14.4], 92.0% of total pb MP), CD45 positive leucocytes/myeloic and lymphatic progenitor cells (0.5x109/l [0.2–1.1], 4.5% of total pb MP), CD235a positive red blood cells/erythropoetic cells (0.2x109/l [0.1–0.6], 1.8% of total pb MP), and CD62e positive endothelial cells (0.1x109/l [0.0–0.1], 0.9% of total pb MP). Mean levels of tissue factor bearing MP were low in bone marrow blood as well as in peripheral blood (0.19 x109/l [0.16 – 0.29] and 0.08 x109/l [0.05 – 0.12], respectively, p=0.004). Conclusion: Within our study we were able to detect MP in bone marrow blood. When comparing MP from bone marrow and peripheral blood, major differences with regard to cell source and concentration was present. Our data suggest that the blood – bone marrow barrier is not only existentant for cells, but also for circulating MP. Disclosures No relevant conflicts of interest to declare.

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P122 CONCENTRATION OF ANGIOGENIC AND OSTEOGENIC GROWTH FACTORS IN PERIPHERAL BLOOD AND BONE MARROW BLOOD OF OSTEONECROSIS PATIENT

Osteoarthritis and Cartilage ◽

10.1016/s1063-4584(07)60574-3 ◽

2006 ◽

Vol 14 ◽

pp. S76-S77 ◽

Cited By ~ 1

Author(s):

S. Sakai ◽

H. Mishima ◽

T. Uemura ◽

H. Akaogi ◽

T. Ogawa ◽

...

Keyword(s):

Bone Marrow ◽

Growth Factors ◽

Peripheral Blood ◽

Bone Marrow Blood

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Neutrophil pseudoplatelets in subgingival plaque and crevicular fluid samples from periodontal diseased sites

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100156298 ◽

1989 ◽

Vol 47 ◽

pp. 862-863 ◽

Cited By ~ 1

Author(s):

J Hanker ◽

E.J. Burkes ◽

G. Greco ◽

R. Scruggs ◽

B. Giammara

Keyword(s):

Electron Microscopy ◽

Bone Marrow ◽

Peripheral Blood ◽

Gingival Crevicular Fluid ◽

Light And Electron Microscopy ◽

Subgingival Plaque ◽

Staining Reaction ◽

Gram Negative Bacteria ◽

Gram Negative ◽

Crevicular Fluid

The mature neutrophil with a segmented nucleus (usually having 3 or 4 lobes) is generally considered to be the end-stage cell of the neutrophil series. It is usually found as such in the bone marrow and peripheral blood where it normally is the most abundant leukocyte. Neutrophils, however, must frequently leave the peripheral blood and migrate into areas of infection to combat microorganisms. It is in such areas that neutrophils were first observed to fragment to form platelet-size particles some of which have a nuclear lobe. These neutrophil pseudoplatelets (NPP) can readily be distinguished from true platelets because they stain for neutrophil myeloperoxidase. True platelets are not positive in this staining reaction because their peroxidase Is inhibited by glutaraldehyde. Neutrophil pseudoplatelets, as well as neutrophils budding to form NPP, could frequently be observed in peripheral blood or bone marrow samples of leukemia patients. They are much more prominent, however, in smears of inflammatory exudates that contain gram-negative bacteria and in gingival crevicular fluid samples from periodontal disease sites. In some of these samples macrophages ingesting, or which contained, pseudoplatelets could be observed. The myeloperoxidase in the ingested pseudoplatelets was frequently active. Despite these earlier observations we did not expect to find many NPP in subgingival plaque smears from diseased sites. They were first seen by light microscopy (Figs. 1, 3-5) in smears on coverslips stained with the PATS reaction, a variation of the PAS reaction which deposits silver for light and electron microscopy. After drying replicate PATS-stained coverslips with hexamethyldisilazane, they were sputter coated with gold and then examined by the SEI and BEI modes of scanning electron microscopy (Fig. 2). Unstained replicate coverslips were fixed, and stained for the demonstration of myeloperoxidase in budding neutrophils and NPP. Neutrophils, activated macrophages and spirochetes as well as other gram-negative bacteria were also prominent in the PATS stained samples. In replicate subgingival plaque smears stained with our procedure for granulocyte peroxidases only neutrophils, budding neutrophils or NPP were readily observed (Fig. 6).

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Bone and Bone Marrow Blood Flow in Blastic Crisis of Chronic Granulocytic Leukaemia

Nuklearmedizin ◽

10.1055/s-0037-1620918 ◽

1979 ◽

Vol 18 (06) ◽

pp. 290-292 ◽

Cited By ~ 2

Author(s):

R. Lahtinen ◽

T. Lahtinen

Keyword(s):

Bone Marrow ◽

Cell Proliferation ◽

Blood Flow ◽

Proximal Femur ◽

Blastic Crisis ◽

Normal Bone ◽

Chronic Granulocytic Leukaemia ◽

Washout Curves ◽

Bone Marrow Blood

SummaryA l33Xe washout method has been used for measuring changes of blood flow in the proximal femur of a patient with the blastic crisis of chronic granulocytic leukaemia. In the hyperplastic phase the blood flow was highly increased and over three times greater than in the hypoplastic phase of the disease and over thirteen times greater than the value in normal bone. The bone circulation and especially the first component of the two-exponential bone washout curves appeared to reflect cell proliferation and neoplastic activity of the whole bone marrow. The method may provide clinically important information in the follow-up of selected haematological diseases.

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