scholarly journals Determination of the Chemical Composition, Antioxidant, and Enzyme Inhibitory Activity of Onosma mollis DC

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Arzuhan Sihoglu Tepe
Keyword(s):  
Chemical Composition ◽  
Inhibitory Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Water Extract ◽  
Reducing Power ◽  
Meoh Extract ◽  
Etoac Extract ◽  
Inhibitory Activities ◽  
Tract Infections

Onosma species have long been used traditionally for respiratory tract infections, abdominal pain, wound treatment, burns, and constipation. This study aims to investigate the chemical composition and in vitro antioxidant and enzyme inhibitory activities of ethyl acetate (EtOAc), methanol (MeOH), and water extracts of Onosma mollis DC. MeOH extract was richer in both phenolics and flavonoids than other extracts (44.06 mg GAEs/g and 41.57 mg QEs/g, respectively). The findings obtained from the results of the chromatographic analysis also supported the results of the spectrophotometric analysis. The MeOH extract was the richest in terms of most of the phytochemicals screened. Apigenin 7-glucoside, luteolin 7-glucoside, rosmarinic acid, vanillic acid, and pinoresinol were over 1000.0 μg/g in MeOH extract. The extract in question showed the highest activity in phosphomolybdenum, DPPH, and ABTS radical scavenging and CUPRAC and FRAP reducing power activity assays (2.01, 3.33, 2.30, 1.48, and 0.79 mg/ml, respectively). The water extract presented the highest activity in the ferrous ion chelating assay (1.01 mg/ml). While EtOAc extract showed high activity in acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and α-glucosidase inhibitory activity tests (1.11, 1.49, and 1.07 mg/ml, respectively), MeOH extract showed significant efficacy in tyrosinase and α-amylase inhibitory activity assays (2.94 and 2.08 mg/ml, respectively). There was a high correlation between the total phenolics/flavonoids of the extracts and their antioxidant activities (correlation coefficients were over 0.9). In addition, the phytochemicals mentioned above were found to contribute significantly to the antioxidant activity. It was concluded that a more detailed analysis should be done to determine the compounds responsible for the enzyme inhibitory activities of the extracts.

scholarly journals Evaluation of the Antioxidant Activities and Tyrosinase Inhibitory Property from Mycelium Culture Extracts

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Ki Moon Park ◽  
Kyung Min Kwon ◽  
Seung Ho Lee
Keyword(s):  
Inhibitory Activity ◽  
Large Scale ◽  
Antioxidant Activities ◽  
Culture Media ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanol Extract ◽  
Scale Production ◽  
Morchella Esculenta

Since mushrooms have many bioactive components, they have been used as components in folk medicine. Because mycelium has an advantage when it comes to large-scale production, this study aimed to evaluate the antioxidant properties and anti-tyrosinase activity from 55 mycelia in culture media. Relatively high 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging capacity was detected from the ethanol extract of culture media including mycelium (EECiM) ofMorchella esculentavar.esculenta(MEVE),Auricularia polytricha(APO),Tremella aurantia(TAU),Volvariella bombycina(VBO), andOudemansiellasp. (Osp), which also showed strong reducing power and inhibitory activity in relation to the thiobarbituric acid (TBA) value. On the other hand, relatively high tyrosinase inhibitory activity was detected inInonotus mikadoi(IMI),Coriolus versicolor(CVE),Volvariella volvacea(VVO),Panellus serotinus(PSE),Auricularia auricula(AAU), andFomitopsissp. (Fsp). Interestingly, the APO EECiM exhibited the highest DPPH radical scavenging rate (77.5 ± 4.3%) and reducing power (1.18 ± 0.041), while the highest inhibitory power of the TBA value and antityrosinase activity were detected in that of TAU (64.5 ± 4.1%) and IMI (46.0 ± 7.5%), respectively. Overall, our study suggested potential candidates for EECiMs that exhibited powerful antioxidant and tyrosinase inhibitory properties and might be used as natural antioxidant tyrosinase inhibitor.

scholarly journals Phytochemical, in-vitro biological and chemo-preventive profiling of Arisaema jacquemontii Blume tuber extracts

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Saira Tabassum ◽  
Muhammad Zia ◽  
Esperanza J. Carcahe de Blanco ◽  
Riffat Batool ◽  
Roohi Aslam ◽  
...  
Keyword(s):  
Cell Lines ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Zone Of Inhibition ◽  
Meoh Extract ◽  
Etoac Extract

Abstract Background Arisaema jacquemontii is traditionally used in treatment of different diseases. In this study, phytochemical, in vitro biological and chemo-preventive screening of A. jacquemontii was carried out to explore its pharmacological potential. Methods The dried tuber of A. jacquemontii was extracted in 11 organic solvent mixture of different polarity. The extracts were screened for phytochemical assays (phenolics and flavonoids), antioxidants potential (free radical scavenging activity, total antioxidant activity, reducing power), biological activities (antibacterial, antifungal, cytotoxic, antileishmanial, protein kinase inhibition), and chemopreventive activities using different cell lines through standard protocols. Results Significant amount phenolic contents were determined in EtOH and MeOH extracts (210.3 ± 3.05 and 193.2 ± 3.15 μg GAE/mg, respectively). Maximum flavonoid content was determined in MeOH extract (22.4 ± 4.04 μg QE/mg). Noteworthy, DPPH scavenging activity was also recorded for MeOH extract (87.66%) followed by MeOH+EtOAc extract (85.11%). Considerable antioxidant capacity (7.8 ± 0.12 μg AAE/mg) and reducing power (3.1 ± 0.15 μg AAE/mg) was observed in extract of MeOH. The LC50 against brine shrimp and leishmanial parasite was found 9.01 and 12.87 μg/mL for n-Hex and CHCl3 extracts, respectively. The highest zone of inhibition against Streptomyces hyphae formation (12.5 ± 1.77 mm) by n-Hex extract. Growth zone of inhibition 13.8 ± 1.08 mm was recorded for EtOAc and MeOH extracts, respectively against Micrococcus luteus while 10.0 ± 0.11 mm for MeOH extract against Aspergillus flavus. In-vitro cytotoxic assay showed that n-Hex extract had higher cytotoxicity against DU-145 prostate cancer and HL-60 cancer cell lines. NF-kB and MTP potential showed 34.01 and 44.87 μg/mL for n-Hex and CHCl3 extracts, respectively in chemo-preventive potential. Conclusion The study concludes that Arisaema jacquemontii bears significant phytochemical activity and pharmacological activities, this plant can be further explored for isolation of active component against a number of aliments.

scholarly journals Potentially Bioactive Metabolites from Pineapple Waste Extracts and Their Antioxidant and α-Glucosidase Inhibitory Activities by 1H NMR

Foods ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 173 ◽  
Author(s):  
Awanis Azizan ◽  
Ai Xin Lee ◽  
Nur Ashikin Abdul Hamid ◽  
Maulidiani Maulidiani ◽  
Ahmed Mediani ◽  
...  
Keyword(s):  
1H Nmr ◽  
Inhibitory Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Ananas Comosus ◽  
Bioactive Metabolites ◽  
Total Phenolic ◽  
Pineapple Waste ◽  
Inhibitory Activities ◽  
Promising Source

Pineapple (Ananas comosus) waste is a promising source of metabolites for therapeutics, functional foods, and cosmeceutical applications. This study strives to characterize the complete metabolite profiles of a variety of MD2 pineapple waste extracts. Metabolomics strategies were utilized to identify bioactive metabolites of this variety prepared with different solvent ratios. Each pineapple waste extract was first screened for total phenolic content, 2,2-diphenyl-1-picrylhydrazyl free radical scavenging, nitric oxide scavenging, and α-glucosidase inhibitory activities. The highest TPC was found in all samples of the peel, crown, and core extracted using a 50% ethanol ratio, even though the results were fairly significant than those obtained for other ethanol ratios. Additionally, crown extracted with a 100% ethanol ratio demonstrated the highest potency in DPPH and NO scavenging activity, with IC50 values of 296.31 and 338.52 µg/mL, respectively. Peel extracted with 100% ethanol exhibited the highest α-glucosidase inhibitory activity with an IC50 value of 92.95 µg/mL. Then, the extracts were analyzed and the data from 1H NMR were processed using multivariate data analysis. A partial least squares and correlogram plot suggested that 3-methylglutaric acid, threonine, valine, and α-linolenic acid were the main contributors to the antioxidant activities, whereas epicatechin was responsible for the α-glucosidase inhibitory activity. Relative quantification further supported that 100% crown extract was among the extracts that possessed the most abundant potential metabolites. The present study demonstrated that the crown and peel parts of MD2 pineapple extracted with 100% ethanol are potentially natural sources of antioxidants and α-glucosidase inhibitors, respectively.

Chemical Composition of Essential Oil and Antioxidant Activities of Curcuma petiolata Roxb. Rhizomes

2012 ◽  
Vol 506 ◽  
pp. 393-396 ◽  
Author(s):  
A. Thakam ◽  
N. Saewan
Keyword(s):  
Ascorbic Acid ◽  
Essential Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Etoac Extract ◽  
Dpph Radical Scavenging

Pulverized rhizome of Curcuma peliolata on hydrodistillation, afforded light yellow essential oil in the yield of 0.13% v/w. The chemical constituent of the essential oil was determined by GC-MS. Nine compounds representing 95.34 % of the total oil were identified. The major compounds of the oil were 2-methyl-5-pentanol, 1H-pyrrol-1-amine,2-(4-methoxyphenyl)-n,n,5-trimethyl, and curcumol. The distillated aqueous was extracted with dicloromethane (DCM), ethyl acetate (EtOAc), and n-butanol. The DPPH radical scavenging and reducing power of the extracts were investigated. Curcumin and ascorbic acid were used as standard references. The DPPH radical scavenging activity was decrease in order: curcumin ascorbic acid EtOAc extract > DCM extract > n-butanol extract > aqueous extract. The EtOAc extract showed significantly highest inhibition of the radical scavenging activity with IC50 0.092 ± 0.001 mg/mL (curcumin and ascorbic acid (IC50 0.012 and 0.013 mg/mL, respectively (P<0.05)). While, the DCM extract showed highest reducing power with IC50 = 0.271 mg/mL which lower than that of standard curcumin (IC50 = 0.013 mg/mL) and ascorbic acid (IC50 = 0.002 mg/mL).Introduction

scholarly journals IN VITRO ANTI-DIABETIC AND ANTI-OXIDANT ACTIVITIES OF ETHANOL EXTRACT OF TINOSPORA SINENSIS

2017 ◽  
Vol 9 (2) ◽  
pp. 42 ◽  
Author(s):  
Anindita Banerjee ◽  
Bithin Maji ◽  
Sandip Mukherjee ◽  
Kausik Chaudhuri ◽  
Tapan Seal
Keyword(s):  
Plant Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Inhibitory Activities ◽  
Ethanol Extracts

Objective: The aim of the present study was to evaluate the alpha (α)-amylase and alpha (α)-glucosidase inhibitory activities and in vitro antioxidant activities of the 80 % aqueous ethanol extracts of Tinosporasinensis Lour (Merr.).Methods: The 80% aq. ethanol extract of the plant was prepared. The plant extract was examined for its antioxidant activity by using free radical 1,1-diphenyl-2-picryl hydrazyl (DPPH) scavenging method, ABTS radical scavenging ability, reducing power capacity, estimation of total phenolic content, flavonoid content and flavonol content. Different concentrations (2, 4, 8,10and 15 μg/ml) of the extract was subjected to α-amylase inhibitory and α-glucosidase inhibitory activities and IC50were calculated.Results: The study revealed that the different concentrations of the plant extract possessed a very good amount of total phenolics, flavonoid and flavonol and exhibited potent radical scavenging activity using DPPH and ABTS as a substrate. The ethanol extracts exhibited significant α-amylase and α-glucosidase inhibitory activities with an IC50 value1.093µg and 1.04µg dry extract respectively and well compared with standard acarbose drug.Conclusion: Thus, it could be concluded that due to the presence of antioxidant components the plant extracts could be used for the treatment of hyperglycemia, diabetes and the related condition of oxidative stress. This knowledge will be useful in finding more potent components from the natural resources for the clinical development of antidiabetic therapeutics.

scholarly journals Antioxidant Activities of Extracts from Sarcocarp of Cotoneaster multiflorus

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Xiangping Liu ◽  
Jia Jia ◽  
Xuemin Jing ◽  
Guoliang Li
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Water Extract ◽  
Reducing Power ◽  
Ethyl Acetate Fraction ◽  
Dependent Manner ◽  
Antioxidant Power ◽  
Water Fraction

The ethanol-water (7 : 3, v/v) extract of Cotoneaster multiflorus sarcocarp was sequentially fractionated by liquid-liquid partition using n-hexane, diethyl ether, methylene dichloride, and ethyl acetate. The contents of total polyphenols, total flavones, and oligomeric proanthocyanidins in the five parts (including the ethanol-water extract) were determined. In addition, 2,2-diphenyl-1-picrylhydrazyl free radical-scavenging, 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical cation decolorization, reducing power, ferric reducing antioxidant power, and lipid peroxidation inhibition assays were conducted to test the antioxidant activities of Sample 1 (the ethanol-water fraction) and Sample 2 (the ethyl acetate fraction) in vitro. In the above five assays, Sample 2 showed greater antioxidant capacities than Sample 1. Furthermore, Sample 2 was better able to protect low-density lipoproteins from oxidation in a dose-dependent manner. The test results show that C. multiflorus sarcocarp, especially the ethyl acetate-soluble fraction, may be a potential source of natural antioxidants.

scholarly journals Antioxidant Profile and Biosafety of White Truffle Mycelial Products Obtained by Solid-State Fermentation

Molecules ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 109
Author(s):  
Jui-Yi Hsu ◽  
Ming-Hsuan Chen ◽  
Yu-Shen Lai ◽  
Su-Der Chen
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Water Extract ◽  
Reducing Power ◽  
Fermentation Products ◽  
White Truffle ◽  
Fermentation Product ◽  
Mycelial Fermentation

Solid-state fermentation may produce therapeutic compounds with higher biomass or better product characteristics than those generated by submerged fermentation. The objectives of this study were to analyze the antioxidant activities and biosafety of products obtained by white truffle (Tuber magnatum) solid-state fermentation in media with different ratios of soybean and red adlay. High levels of antioxidant components and high antioxidant activities such as DPPH radical scavenging, ferrous ion chelation, and reducing power were measured in 20 mg/mL water and ethanol extracts of the white truffle fermentation products. When the solid-state fermentation medium contained soybean and red adlay in a 1:3 ratio (S1A3), the fermentation product had more uniform antioxidant compositions and activities by principal component analysis (PCA). In addition, a 200 ppm water extract of the mycelial fermentation product was able to protect zebrafish embryos from oxidative stress induced by 5 mM hydrogen peroxide. Sprague–Dawley rats were fed the mycelial fermentation product for 90 consecutive days, revealing a no-observed-adverse-effect level (NOAEL) of 3000 mg/kg BW/day. Therefore, mycelial products obtained by white truffle solid-state fermentation can be used instead of expensive fruiting bodies as a good source of antioxidant ingredients.

scholarly journals Phytochemical analysis and antimicrobial and antioxidant activities of Hen-riettea succosa (Aubl.) DC. leaves

2021 ◽  
Vol 9 (4) ◽  
pp. 340-349
Author(s):  
Izaqueu Rodrigues Da Silva ◽  
Bruno Anderson de Morais ◽  
Maria Isabela Ferreira De Araújo ◽  
Paula Perazzo de Souza Barbosa ◽  
Amanda Reges de Sena ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Micrococcus Luteus ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Spectrophotometric Analysis ◽  
Aureus Strain ◽  
Phytochemical Analysis ◽  
Meoh Extract ◽  
Minimum Concentration

Henriettea succosa is a tree species consumed in abundance by birds, however, there is no report on its phytochemical profile and biological activity. This study performed the phytochemical screening and the antimicrobial and antioxidant potential of H. succosa leaves. The hexane (Hex), ethyl acetate (AcOEt) and methanol (MeOH) extracts of the leaves were evaluated for chemical composition by Thin Layer Chromatography and spectrophotometric analysis; the antimicrobial activity was determined by the Minimum Inhibitory Concentration (MIC) and Minimum Microbicide Concentration (MMC); antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, determination of the reducing power and the phosphomolybdenum complex reduction assay. The photoprotor action of the extracts was also evaluated. The results showed a higher content of phenolic compounds (444.08 ± 0.020 mg EAG/g) and tannins (414.37 ± 0.16 mg EAG/g) in the MeOH extract, which was effective against Staphylococcus aureus and Serratia marcescens, with MIC of 1 mg/ ml and CMM of 2 mg/ml. The MIC and MMC of AcOEt for Micrococcus luteus was 1 mg/mL, this was also considered the minimum concentration necessary for the Hex extract to act on the S. aureus strain. The MeOH extract showed greater antioxidant activity by the DPPH (79.09%) and reducing power (327.2 ± 0.00 mg EAA/g) methods, while the AcOEt extract showed greater activity by the phosphomolybdenum method (40.5%). However, none of the extracts showed a photoprotective effect against UV radiation. In summary, this study revealed that the leaves of H. succosa have secondary metabolites with bactericidal potential, in addition to antioxidant action.

scholarly journals PHYTOCHEMICAL EVALUATION AND IN VITRO ANTIMICROBIAL AND ANTIOXIDANT STUDIES OF LEAF AND STEM BARK EXTRACTS OF POLYALTHIA FRAGRANS (DALZ.) BEDD.–AN ENDEMIC SPECIES OF WESTERN GHATS

2017 ◽  
Vol 9 (8) ◽  
pp. 20
Author(s):  
Arunkumar K. ◽  
K. R. Chandrashekar
Keyword(s):  
Bacillus Subtilis ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Water Extract ◽  
Reducing Power ◽  
Inhibition Zone ◽  
Stem Bark ◽  
Diffusion Method

Objective: Phytochemical constituents of various solvent extracts of leaf and stem bark of Polyalthia fragrans (Dalz.) Bedd were screened. Antimicrobial and antioxidant activities of the extracts were evaluated.Methods: Crude extracts of methanol, ethyl acetate, chloroform and aqueous of leaf and stem bark were evaluated for antimicrobial activity by disc diffusion method and antioxidant activity by DPPH (diphenyl-2-picrylhydrazyl) radical scavenging and reducing power assay. Quantitative analysis of total phenolics was done by the Folin-Ciocalteau method and total flavonoids by aluminum chloride method.Results: The study revealed the presence of several physiologically active phytochemicals such as alkaloids, phenols, flavonoids and tannins. The highest antibacterial activity of methanol extract of the leaf was observed against Bacillus subtilis with 14.33±0.57 mm inhibition zone and water extract of stem bark against Proteus vulgaris with 17.67±1.52 mm inhibition zone among other extracts. The lowest activity of leaf methanol and leaf water extract was observed against Bacillus subtilis. The IC50 values of 58.18±2.04 µg/ml, 116.58±2.43 µg/ml, 134.78±3.64 µg/ml and 387.28±2.9 µg/ml for DPPH activity were observed in methanol stem bark, ethyl acetate stem bark, methanol leaf and ethyl acetate leaf extracts respectively. The reducing power of extracts was very potent which increased with increasing concentrations of the sample.Conclusion: The results indicate that phytochemicals like alkaloids, phenols, flavonoids and tannins may be responsible for the antimicrobial and antioxidant properties. The results suggest that P. fragrans can be further researched to be used as a natural source of a potent drug by mankind.

scholarly journals Phenolic compounds profile, neuroprotective effect and antioxidant potential of a commercial Turkish coffee

2020 ◽  
Vol 33 ◽  
Author(s):  
Melek ÇOL AYVAZ
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Neuroprotective Effect ◽  
Radical Scavenging ◽  
Water Extract ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Activity

ABSTRACT Objective The purpose of this study is to determine the phenolic and flavonoid contents, and antioxidant activities and neuroprotective effects of powdered coffee sample of a commercial coffee brand originated from Sivas, Turkey. Methods Total phenolic, flavonoid and antioxidant contents, enzymatic and non-enzymatic antioxidative activities based on 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity, metal chelating potential, reducing power, superoxide dismutase and catalase activity tests and lipid peroxidation inhibition potentials of the ethanolic and aqueous extracts of the coffee sample were assayed using the commonly preferred spectrophotometric methods. Furthermore the extracts’ cholinesterase and tyrosinase inhibition potentials were evaluated. Phenolic profiles of the coffee sample were investigated using high performance liquid chromatography. Results Catechin was the most frequently detected phenolic acid. In addition, it was demonstrated that the water extract has a significant impact when compared with standard antioxidants. While the SC50 (sufficient concentration to obtain 50% of a maximum scavenging capacity) value for the scavenging activity of 2,2-diphenyl-1-picrylhydrazyl free radical was calculated as being 0.08mg/mL for water extract, the amount of chelating agents with half Fe2+ ions in the medium was found to be 0.271mg/mL. Additionally, it was shown that 0.1mg/mL concentration of both extracts prevents lipid peroxidation by 8%. Compared with standard drugs, inhibition potentials of cholinesterase and tyrosinase enzymes were considered as moderately acceptable in these samples. Conclusion Besides the extracts’ enzymatic antioxidant activity, their inhibition potential on cholinesterase and tyrosinase enzymes – which are important clinical enzymes – reveal that this natural source can be used as a valuable resource in different fields, especially in medicine.

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