scholarly journals The Development of an Amperometric Enzyme Biosensor Based on a Polyaniline-Multiwalled Carbon Nanocomposite for the Detection of a Chemotherapeutic Agent in Serum Samples from Patients

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Ahmad Gholami ◽  
Fatemeh Farjami ◽  
Younes Ghasemi
Keyword(s):  
Anticancer Agent ◽  
Platinum Electrode ◽  
Chemotherapeutic Agent ◽  
Ammonium Ion ◽  
Serum Samples ◽  
Multiwalled Carbon ◽  
Enzyme Biosensor ◽  
Menten Constant ◽  
Carbon Nanocomposite

Polyaniline was electrochemically polymerized onto the platinum electrode modified with a mixture of Nafion and multiwalled carbon nanotube (PANI/MWCNT/Nf/Pt) to detect ammonium ion. The nanobiosensor (ASNase/PANI/MWCNT/Nf/Pt) was then prepared by immobilizing L-asparaginase (L-ASNase) on the PANI/MWCNT/Nf nanocomposite. The prepared nanobiosensor was used for the rapid and sensitive detection of serum concentration of the anticancer agent L-asparagine (L-Asp) during chemotherapy. The nanobiosensor has dynamic ranges of zero to 180 μM. The sensitivity of the nanobiosensor was 0.829 μA μM−1 cm−2, and the response time was less than 30 s. The detection limit was 140 nM of L-Asp. The Michaelis–Menten constant ( K m ) was measured to be 36.2 mM. The nanobiosensor was successfully applied for the determination of L-Asp in the blood samples of leukemia patients.

scholarly journals Conductimetric Biosensor for the Detection of Uric Acid by Immobilization Uricase on Nata de Coco Membrane—Pt Electrode

2011 ◽  
Vol 6 ◽  
pp. ACI.S7346 ◽  
Author(s):  
Ani Mulyasuryani ◽  
Arie Srihardiastutie
Keyword(s):  
Uric Acid ◽  
Human Serum ◽  
Candida Utilis ◽  
Medical Laboratory ◽  
Membrane Thickness ◽  
Serum Samples ◽  
Ph Change ◽  
Pt Electrode ◽  
Enzyme Biosensor

A conductimetric enzyme biosensor for uric acid detection has been developed. The uricase, as enzyme, is isolated from Candida utilis and immobilized on a nata de coco membrane-Pt electrode. The biosensor demonstrates a linear response to urate over the concentration range 1-6 ppm and has good selectivity properties. The response is affected by the membrane thickness and pH change in the range 7.5-9.5. The response time is three minutes in aqueous solutions and in human serum samples. Application of the biosensor to the determination of uric acid in human serum gave results that compared favourably with those obtained by medical laboratory. The operational stability of the biosensor was not less than three days and the relative error is smaller than 10%.

Highly sensitive determination of perphenazine on a carbon nanocomposite ionic liquid electrode

RSC Advances ◽  
2015 ◽  
Vol 5 (115) ◽  
pp. 95087-95095 ◽  
Author(s):  
Farshid Fasihi ◽  
Fatemeh Farjami ◽  
Gholam Hossein Shafiee
Keyword(s):  
Ionic Liquid ◽  
Carbon Nanotube ◽  
Blood Serum ◽  
Electrochemical Detection ◽  
Serum Samples ◽  
Multi Walled Carbon Nanotube ◽  
Highly Sensitive ◽  
Sensitive Determination ◽  
Carbon Nanocomposite

A multi-walled carbon nanotube–ionic liquid nanocomposite was fabricated for the electrochemical detection of perphenazine, suitable for the analysis of pharmaceuticals and blood serum samples.

Electroanalytical determination of diazepam in tablet and human serum samples using a multiwalled carbon nanotube embedded molecularly imprinted polymer-modified carbon paste electrode

RSC Advances ◽  
2015 ◽  
Vol 5 (99) ◽  
pp. 81650-81659 ◽  
Author(s):  
Mohammad Reza Milani Hosseini ◽  
Ali Motaharian
Keyword(s):  
Carbon Nanotube ◽  
Carbon Paste Electrode ◽  
Modified Carbon Paste Electrode ◽  
Carbon Paste ◽  
Imprinted Polymer ◽  
Serum Samples ◽  
Multiwalled Carbon ◽  
Molecularly Imprinted ◽  
Paste Electrode

In this study a new MWCNT–MIP nanocomposite modified carbon paste electrode for determination of diazepam has been developed.

scholarly journals A Screen-Printed Sensor Coupled with Flow System for Quantitative Determination of a Novel Promising Anticancer Agent Candidate

Sensors ◽  
2020 ◽  
Vol 20 (18) ◽  
pp. 5217
Author(s):  
Katarzyna Tyszczuk-Rotko ◽  
Jędrzej Kozak ◽  
Małgorzata Sztanke ◽  
Krzysztof Sztanke ◽  
Ilona Sadok
Keyword(s):  
Quantitative Determination ◽  
High Performance ◽  
Square Wave Voltammetry ◽  
Anticancer Agent ◽  
Comparative Method ◽  
Quadrupole Mass ◽  
Serum Samples ◽  
Wave Voltammetry ◽  
Screen Printed

A carbon nanofibers modified screen-printed carbon sensor (SPCE/CNFs) was applied for the determination of a novel promising anticancer agent candidate (ethyl 8-(4-methoxyphenyl)-4-oxo-4,6,7,8-tetrahydroimidazo[2,1-c][1,2,4]triazine-3-carboxylate, EIMTC) using square-wave voltammetry (SWV). It is the first method for the quantitative determination of EIMTC. The modified screen-printed sensor exhibited excellent electrochemical activity in reducing EIMTC. The peak current of EIMTC was found to be linear in two concentration ranges of 2.0 × 10−9 – 2.0 × 10−8 mol L−1 and 2.0 × 10−8 – 2.0 × 10−7 mol L−1, with a detection limit of 5.0 × 10−10 mol L−1. The connection of flow-cell for the SPCE/CNFs with SWV detection allowed for the successful determination of EIMTC in human serum samples. Ultra-high-performance liquid chromatography coupled to electrospray ionization triple quadrupole mass spectrometry (UHPLC-ESI-MS/MS) acted as a comparative method in the serum samples analysis.

Preparation, characterization and analytical application of an electrochemical laccase biosensor towards low level determination of isoprenaline in human serum samples

RSC Advances ◽  
2014 ◽  
Vol 4 (101) ◽  
pp. 57591-57599 ◽  
Author(s):  
P. Gopal ◽  
T. Madhusudana Reddy ◽  
C. Nagaraju ◽  
G. Narasimha
Keyword(s):  
Carbon Nanotubes ◽  
Human Serum ◽  
Multiwalled Carbon Nanotubes ◽  
Electrochemical Biosensor ◽  
Sol Gel ◽  
Analytical Application ◽  
Serum Samples ◽  
Multiwalled Carbon ◽  
Gel Entrapment

A novel electrochemical biosensor has been developed based on the immobilization of multiwalled carbon nanotubes (MWCNT) followed by sol–gel entrapment of laccase (Lac) enzyme on to the GCE.

The Therapeutic and Diagnostic Value of Fluorine

2020 ◽  
Vol 4 (1) ◽  
pp. 17-29
Author(s):  
Isma Attique ◽  
Shabbir Hussain ◽  
Muhammad Amjad ◽  
Khalida Nazir ◽  
Muhammad Shahid Nazir
Keyword(s):  
Anticancer Agent ◽  
Chemotherapeutic Agent ◽  
Functional Materials ◽  
Diagnostic Value ◽  
Vital Role ◽  
Malignant Growth ◽  
Medical Field ◽  
Active Nucleus ◽  
Broad Application ◽  
Tracer Atom

Fluorine has a useful positron transmitting isotope and it enjoys broad application in the medical field. It is utilized in fluorinated agents,therapeutic sciences and steroid field. Fluorine incorporation viafluoroalkylation is a useful approach in the development of new functional materials and in drug design. Fluorine also plays its role as an anticancer agent and is a successful chemotherapeutic agent for certain sorts of malignant growth. 5-fluorouracil plays a vital role in the treatment of cancer. 18 Facts as a radio label tracer atom in PET imaging. 19 F has the second most sensitive and stable NMR-active nucleus.

The Dtermination of Paracetamol by HPLC Validation of the Method and Application on Serum Samples

2018 ◽  
Vol 69 (3) ◽  
pp. 627-631 ◽  
Author(s):  
Viorica Ohriac (Popa) ◽  
Diana Cimpoesu ◽  
Adrian Florin Spac ◽  
Paul Nedelea ◽  
Voichita Lazureanu ◽  
...  
Keyword(s):  
Mobile Phase ◽  
High Performance ◽  
Hplc Analysis ◽  
Emotional Experience ◽  
Optimum Conditions ◽  
Serum Samples ◽  
Liquid Chromatograph ◽  
Mobile Phase Flow Rate ◽  
Quantification Limit

Pain is defined as a disagreeable sensory and emotional experience related to a tissue or potential lesion. Paracetamol (Acetaminophen) is the most used non-morphine analgesic. For the determination of paracetamol we developed and validated the high performance liquid chromatography (HPLC) analysis using a Dionex Ultimate 3000 liquid chromatograph equipped with a multidimensional detector. After determining the optimum conditions of analysis (80/20 water / acetonitrile mobile phase, flow rate 1.0 mL / min, detection wavelength 245 nm) we validated the method following the following parameters: linearity of response function, linearity of results, limit (LD = 0.66 mg / mL) and quantification limit (LQ = 2.00 mg / mL), and precision. The method of determining paracetamol by HPLC was applied to 30 samples of serum collected from patients who had pain and were treated with paracetamol.

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