scholarly journals Ascorbic Acid Significantly Decreases Creatine Kinase Plasma Levels in an Animal Model of Statin/Fibrate-Induced Myopathy

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Mohsen Zabihi ◽  
Fatemeh Askarian ◽  
Seyedhossein Hekmatimoghaddam ◽  
Mohammadreza Rashidi Nooshabadi ◽  
Mohammad Sajjad Zabihi ◽  
...  
Keyword(s):  
Animal Model ◽  
Ascorbic Acid ◽  
Creatine Kinase ◽  
Lactate Dehydrogenase ◽  
Plasma Levels ◽  
Lipid Lowering ◽  
Blood Levels ◽  
Exercise Regimen ◽  
Muscular Disease ◽  
Vit C

Background. Myopathy is one of the side effects of lipid-lowering drugs, especially statins and particularly when combined with a fibrate. To diagnose myopathy and determine its severity, the plasma levels of three enzymes, creatine kinase (CK), aldolase, and lactate dehydrogenase (LDH), are routinely measured. Physical exercise can aggravate the statin-associated muscular disease. The question is whether antioxidants like ascorbic acid (Vit. C) can prevent such myopathy. Methods. In this experiment, a combination of atorvastatin (ATV, 80 mg/kg/day) and gemfibrozil (GMF, 1000 mg/kg/day) orally for 10 days as well as exercise as forced swimming on days 8, 9, and 10 were used to induce myopathy. Ascorbic acid (50 mg/kg/day, orally) was added to ATV/GMF plus exercise regimen throughout the 10 days in the treatment group. Mean blood levels of CK, aldolase, and LDH were measured in addition to swimming tolerance times. Results. There was a significantly higher swimming tolerance time P < 0.05 and lower CK levels P < 0.01 in rats receiving ATV/GMF/Vit. C plus exercise compared with rats not taking Vit. C. LDH and aldolase did not decrease significantly. Conclusion. The results of this study showed that Vit. C can be effective in preventing myopathy caused by fat-lowering drugs.

scholarly journals Ascorbic Acid Greatly Decreases Creatine Kinase Levels in An Animal Model of Statin/Fibrate-Induced Myopathy

2021 ◽  
Author(s):  
Fatemeh Askarian ◽  
Mohsen Zabihi ◽  
Seyedhossein Hekmatimoghaddam ◽  
Mohammadreza Rashidi Nooshabadi ◽  
Mohammad sajjad Zabihi
Keyword(s):  
Ascorbic Acid ◽  
Creatine Kinase ◽  
Physical Exercise ◽  
Protective Role ◽  
Muscle Disease ◽  
Blood Levels ◽  
Drug Induced ◽  
Exercise Regimen ◽  
Muscular Disease ◽  
Swimming Test

Abstract BackgroundDrug-induced myopathy is one of the frequent forms of muscle disease, and drugs used for hyperlipidemia, especially the statins are a common culprit, and particularly when combined with a fibrate. Clinicians usually measure plasma levels of three enzymes, creatine kinase (CK), aldolase and lactate dehydrogenase (LDH) for diagnosis of myopathy and determination of its severity. Physical exercise can aggravate statin-associated muscular disease. The question is whether antioxidants like ascorbic acid (vit. C) can prevent such myopathy.MethodsIn this experiment a combination of oral atorvastatin (ATV, 80 mg/kg/day, orally) and gemfibrozil (GMF, 1000 mg/kg/day, orally) was used for ten days plus exercise in days 8, 9 and 10 to induce myopathy in rats. To add physical exercise, the forced swimming test was applied in the last three days. Ascorbic acid (50 mg/kg/day, orally) was added to ATV/GMF plus exercise regimen throughout the 10 days in the treatment group. The mean blood levels of CK, aldolase and LDH were measured in addition to swimming tolerance times. ResultsThere was a significantly lower swimming tolerance time (P < 0.05) and higher CK levels (P < 0.01) in rats receiving ATV/GMF/Vit.C plus exercise compared with rats not taking Vit.C. LDH and aldolase didn’t decrease significantly.ConclusionsA protective role of vit.C against drug-induced myopathy is suggested by the findings of this study.

Correlation Between Platelet Disaggregation And Arachidonic Acid Conversion To Prostacyclin And Thromboxane A2 In The Ex Vivo Cat Model

1981 ◽  
Author(s):  
N S Nicholson ◽  
S L Smith ◽  
R N Saunders
Keyword(s):  
Animal Model ◽  
Arachidonic Acid ◽  
Plasma Levels ◽  
Ex Vivo ◽  
Thromboxane A2 ◽  
Blood Levels ◽  
Platelet Disaggregation ◽  
Cat Model ◽  
Peak Blood ◽  
Observation Period

Arachidonic Acid (AA) infusion in the ex vivo cat model was performed to determine the effects of the products produced from the metabolism of AA on the disaggregation of platelets accumulated on a rabbit Achilles tendon. Apparent peak blood levels of both prostacyclin (PGI2) and thromboxane (TXA2) were noted 4-8 minutes post initiation of AA infusion (1.0 mpk, i.v.). Plasma levels of PGI2 and TXA2 were determined by radioimmunoassay. Significant platelet disaggregation was noted 6-8 minutes post AA infusion with peak effects attained at 20-24 minutes. In other experiments no disaggregation occurred when AA was infused at 0.5 mg/kg. PGI2 infused in the same animal model at 14 μg/kg was detected in peak levels within 2 minutes after infusion and disappeared rapidly thereafter. Disaggregation of accumulated platelets following PGI2 infusion occurred immediately after the completion of infusion and continued over the 20 minute observation period. This data shows that AA infused into the cat can be converted to both PGI2 and TXA2 and that at 1.0 mg/kg AA, sufficient PGI2 is produced to result in platelet disaggregation even in the presence of elevated TXA2 levels.

Preferential uptake of intravenously administered hyaluronidase (Hyalosidase) by damaged rat myocardium

1985 ◽  
Vol 5 (4) ◽  
pp. 329-334
Author(s):  
Julia S. Earnshaw ◽  
Kheng H. Peh ◽  
Kenneth S. Dodgson ◽  
Anthony H. Olavesen ◽  
Peter Gacesa
Keyword(s):  
Myocardial Infarction ◽  
Creatine Kinase ◽  
Coronary Artery ◽  
Lactate Dehydrogenase ◽  
Plasma Levels ◽  
Heart Tissue ◽  
Model System ◽  
Rat Myocardium ◽  
Normal Heart ◽  
Preferential Uptake

The induction of myocardial infarction in rats by ligation of the left – anterior coronary artery was confirmed by measurement of increased plasma levels of creatine kinase, aspartate aminotransferase and lactate dehydrogenase. Using this model system it has been established that intravenous administration of 12SI-labelled hyaluronidase to rats resulted in a preferential uptake of the enzyme by damaged myocardium as compared to normal heart tissue.

99mTc-Aminohexylidendiphosphonate and 99mTc-Pyrophos phate in the Scintigraphic Diagnosis of Experimental Cardiomyopathy in Dogs

1987 ◽  
Vol 26 (05) ◽  
pp. 220-223 ◽  
Author(s):  
L. Hadaš ◽  
J. VižĎa ◽  
P. Kafka ◽  
Y. Mazurová ◽  
V. Palicka ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Plasma Levels ◽  
Maximum Accumulation ◽  
Kinase A ◽  
Good Agreement

Experimental cardiomyopathy was provoked in 24 dogs with high intravenous doses of adrenaline and theophylline. These lesions were studied by means of the new agent 99mTc-AHDP and 99mTc-PYP in comparison. Cardiomyopathy could be imaged as early as 4 h after the onset of involvement but not later than 7 days. A maximum accumulation occurred in lesions 24 h old. 99mTc uptake in the myocardium was graded scintigraphically. 99mTc-AHDP was accumulated in the altered’myocardium to a greater extent than 99mTc-PYP. Scintigraphic findings were in good agreement with plasma levels of creatine- kinase. A comparison with histology demonstrated that the maximum accumulation of radiopharmaceuticals occurred at the time when the development of myocardium involvement reached the stage of myocytolysis.

EFFECT OF DRAFT LOAD ON LACTATE DEHYDROGENASE AND CREATINE KINASE ACTIVITIES IN MULES.(Equusasinus x Equuscaballus)

2016 ◽  
Vol 12 (1) ◽  
Author(s):  
A. Moolchandani ◽  
M. Sareen
Keyword(s):  
Creatine Kinase ◽  
Lactate Dehydrogenase ◽  
Serum Creatine Kinase

This study was carried out to assess the effects of draft load on serum creatine kinase and lactated ehydorgenase activities in mule. Five adult mules of 5 to 6 years of age were subjected to loading exercise (i.e. 10% draft load for 1 to 5 days and 20% draft load from 6th to 10th day). A highly significant (P<0.01) increase in serum LDH activities in response to 10% and 20% draft load exercise was observed while significant (P<0.05) increase at 10% draft load and highly significant (P<0.01) increase at 20% draft load was observed in CK activities.

Production and certification of secondary enzyme reference materials (ERMs). Part 1: Preparation of the sera and some of their properties.

1986 ◽  
Vol 32 (10) ◽  
pp. 1901-1905 ◽  
Author(s):  
J C Koedam ◽  
G M Steentjes ◽  
S Buitenhuis ◽  
E Schmidt ◽  
R Klauke
Keyword(s):  
Creatine Kinase ◽  
Lactate Dehydrogenase ◽  
Reference Material ◽  
Human Serum ◽  
Dehydrogenase Activity ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Glutamate Dehydrogenase Activity ◽  
The Stability ◽  
Clinical Enzymology

Abstract We produced three batches of a human-serum-based enzyme reference material (ERM) enriched with human aspartate aminotransferase (EC 2.6.1.1), alanine aminotransferase (EC 2.6.1.2), creatine kinase (EC 2.7.3.2), and lactate dehydrogenase (EC 1.1.1.27). The added enzymes were not exhaustively purified; thus the final ERMs contained some enzymes as contaminants, of which only glutamate dehydrogenase activity might interfere. The stability during storage and after reconstitution was good. The commutability of the four enzymes in the three ERM batches was also good, except when German or Scandinavian methods for aminotransferases were involved. The temperature-conversion factors for the ERMs were equivalent to those for patients' sera. Reactivation after reconstitution was complete within 5 min and was independent of the temperature of the reconstitution fluid. We believe that these secondary ERMs will aid in the transfer of accuracy between well-defined reference methods and daily working methods so that clinical enzymology results will become more comparable from laboratory to laboratory.

scholarly journals Interference of ascorbic acid with chemical analytes

2005 ◽  
Vol 42 (6) ◽  
pp. 475-477 ◽  
Author(s):  
Qing H Meng ◽  
William C Irwin ◽  
Jennifer Fesser ◽  
K Lorne Massey
Keyword(s):  
Ascorbic Acid ◽  
Creatine Kinase ◽  
Uric Acid ◽  
Total Cholesterol ◽  
Acid Concentration ◽  
Total Bilirubin ◽  
Binding Capacity ◽  
Ascorbic Acid Concentration ◽  
Sodium Potassium ◽  
Creatine Kinase Mb

Background: Ascorbic acid can interfere with methodologies involving redox reactions, while comprehensive studies on main chemistry analysers have not been reported. We therefore attempted to determine the interference of ascorbic acid with analytes on the Beckman Synchron LX20®. Methods: Various concentrations of ascorbic acid were added to serum, and the serum analytes were measured on the LX20. Results: With a serum ascorbic acid concentration of 12.0 mmol/L, the values for sodium, potassium, calcium and creatinine increased by 43%, 58%, 103% and 26%, respectively ( P<0.01). With a serum ascorbic acid concentration of 12.0 mmol/L, the values for chloride, total bilirubin and uric acid decreased by 33%, 62% and 83%, respectively ( P<0.01), and were undetectable for total cholesterol, triglyceride, ammonia and lactate. There was no definite influence of ascorbic acid on analytical values for total CO2, urea, glucose, phosphate, total protein, albumin, amylase, creatine kinase, creatine kinase-MB, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total iron, unbound iron-binding capacity or magnesium. Conclusions: Ascorbic acid causes a false increase in sodium, potassium, calcium and creatinine results and a false decrease in chloride, total bilirubin, uric acid, total cholesterol, triglyceride, ammonia and lactate results.

Ascorbic Acid Intakes and Plasma Levels in Healthy Elderly

1987 ◽  
Vol 498 (1 Third Confere) ◽  
pp. 90-99 ◽  
Author(s):  
PHILIP J. GARRY ◽  
DOROTHY J. VANDERJAGT ◽  
WILLIAM C. HUNT
Keyword(s):  
Ascorbic Acid ◽  
Plasma Levels ◽  
Healthy Elderly
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