Ascorbic Acid Greatly Decreases Creatine Kinase Levels in An Animal Model of Statin/Fibrate-Induced Myopathy

Abstract BackgroundDrug-induced myopathy is one of the frequent forms of muscle disease, and drugs used for hyperlipidemia, especially the statins are a common culprit, and particularly when combined with a fibrate. Clinicians usually measure plasma levels of three enzymes, creatine kinase (CK), aldolase and lactate dehydrogenase (LDH) for diagnosis of myopathy and determination of its severity. Physical exercise can aggravate statin-associated muscular disease. The question is whether antioxidants like ascorbic acid (vit. C) can prevent such myopathy.MethodsIn this experiment a combination of oral atorvastatin (ATV, 80 mg/kg/day, orally) and gemfibrozil (GMF, 1000 mg/kg/day, orally) was used for ten days plus exercise in days 8, 9 and 10 to induce myopathy in rats. To add physical exercise, the forced swimming test was applied in the last three days. Ascorbic acid (50 mg/kg/day, orally) was added to ATV/GMF plus exercise regimen throughout the 10 days in the treatment group. The mean blood levels of CK, aldolase and LDH were measured in addition to swimming tolerance times. ResultsThere was a significantly lower swimming tolerance time (P < 0.05) and higher CK levels (P < 0.01) in rats receiving ATV/GMF/Vit.C plus exercise compared with rats not taking Vit.C. LDH and aldolase didn’t decrease significantly.ConclusionsA protective role of vit.C against drug-induced myopathy is suggested by the findings of this study.

Download Full-text

Ascorbic Acid Significantly Decreases Creatine Kinase Plasma Levels in an Animal Model of Statin/Fibrate-Induced Myopathy

Advances in Pharmacological and Pharmaceutical Sciences ◽

10.1155/2021/5539595 ◽

2021 ◽

Vol 2021 ◽

pp. 1-7

Author(s):

Mohsen Zabihi ◽

Fatemeh Askarian ◽

Seyedhossein Hekmatimoghaddam ◽

Mohammadreza Rashidi Nooshabadi ◽

Mohammad Sajjad Zabihi ◽

...

Keyword(s):

Animal Model ◽

Ascorbic Acid ◽

Creatine Kinase ◽

Lactate Dehydrogenase ◽

Plasma Levels ◽

Lipid Lowering ◽

Blood Levels ◽

Exercise Regimen ◽

Muscular Disease ◽

Vit C

Background. Myopathy is one of the side effects of lipid-lowering drugs, especially statins and particularly when combined with a fibrate. To diagnose myopathy and determine its severity, the plasma levels of three enzymes, creatine kinase (CK), aldolase, and lactate dehydrogenase (LDH), are routinely measured. Physical exercise can aggravate the statin-associated muscular disease. The question is whether antioxidants like ascorbic acid (Vit. C) can prevent such myopathy. Methods. In this experiment, a combination of atorvastatin (ATV, 80 mg/kg/day) and gemfibrozil (GMF, 1000 mg/kg/day) orally for 10 days as well as exercise as forced swimming on days 8, 9, and 10 were used to induce myopathy. Ascorbic acid (50 mg/kg/day, orally) was added to ATV/GMF plus exercise regimen throughout the 10 days in the treatment group. Mean blood levels of CK, aldolase, and LDH were measured in addition to swimming tolerance times. Results. There was a significantly higher swimming tolerance time P < 0.05 and lower CK levels P < 0.01 in rats receiving ATV/GMF/Vit. C plus exercise compared with rats not taking Vit. C. LDH and aldolase did not decrease significantly. Conclusion. The results of this study showed that Vit. C can be effective in preventing myopathy caused by fat-lowering drugs.

Download Full-text

Interference of ascorbic acid with chemical analytes

Annals of Clinical Biochemistry International Journal of Laboratory Medicine ◽

10.1258/000456305774538274 ◽

2005 ◽

Vol 42 (6) ◽

pp. 475-477 ◽

Cited By ~ 15

Author(s):

Qing H Meng ◽

William C Irwin ◽

Jennifer Fesser ◽

K Lorne Massey

Keyword(s):

Ascorbic Acid ◽

Creatine Kinase ◽

Uric Acid ◽

Total Cholesterol ◽

Acid Concentration ◽

Total Bilirubin ◽

Binding Capacity ◽

Ascorbic Acid Concentration ◽

Sodium Potassium ◽

Creatine Kinase Mb

Background: Ascorbic acid can interfere with methodologies involving redox reactions, while comprehensive studies on main chemistry analysers have not been reported. We therefore attempted to determine the interference of ascorbic acid with analytes on the Beckman Synchron LX20®. Methods: Various concentrations of ascorbic acid were added to serum, and the serum analytes were measured on the LX20. Results: With a serum ascorbic acid concentration of 12.0 mmol/L, the values for sodium, potassium, calcium and creatinine increased by 43%, 58%, 103% and 26%, respectively ( P<0.01). With a serum ascorbic acid concentration of 12.0 mmol/L, the values for chloride, total bilirubin and uric acid decreased by 33%, 62% and 83%, respectively ( P<0.01), and were undetectable for total cholesterol, triglyceride, ammonia and lactate. There was no definite influence of ascorbic acid on analytical values for total CO2, urea, glucose, phosphate, total protein, albumin, amylase, creatine kinase, creatine kinase-MB, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total iron, unbound iron-binding capacity or magnesium. Conclusions: Ascorbic acid causes a false increase in sodium, potassium, calcium and creatinine results and a false decrease in chloride, total bilirubin, uric acid, total cholesterol, triglyceride, ammonia and lactate results.

Download Full-text

Toxic effects of lambda-cyhalothrin, a synthetic pyrethroid pesticide, on the rat kidney: Involvement of oxidative stress and protective role of ascorbic acid

Toxicology Letters ◽

10.1016/j.toxlet.2010.03.211 ◽

2010 ◽

Vol 196 ◽

pp. S53 ◽

Cited By ~ 1

Author(s):

H. Fetoui ◽

N. Zeghal

Keyword(s):

Oxidative Stress ◽

Ascorbic Acid ◽

Rat Kidney ◽

Protective Role ◽

Synthetic Pyrethroid ◽

Toxic Effects ◽

Lambda Cyhalothrin ◽

Pyrethroid Pesticide ◽

Kidney Involvement

Download Full-text

Interaction between physical exercise and ascorbic acid supplementation on the histamine tissue of skeletal and cardiac muscles of sedentary and trained guinea-pigs

Revista Paulista de Educação Física ◽

10.11606/issn.2594-5904.rpef.1997.138563 ◽

1997 ◽

Vol 11 (2) ◽

pp. 134

Author(s):

Maria Cecília Bueno Jayme Gallani ◽

José Roberto Moreira Azevedo ◽

Marilia Mantovani Sampaio Barros ◽

Rui Errerias Maciel

Keyword(s):

Ascorbic Acid ◽

Physical Exercise ◽

Guinea Pigs ◽

Cavia Porcellus ◽

Acid Supplementation ◽

Cardiac Muscles ◽

Ascorbic Acid Supplementation

O presente estudo objetivou avaliar em cobaias (“Cavia porcellus”), fêmeas, com três meses de idade, sedentárias (SED) e treinadas (TRE = natação, 30 min/dia, durante sete semanas) o comportamento da concentração tecidual de histamina muscular e cardíaca, em resposta ao exercício agudo (E = exercício agudo - natação, 30 minutos, R = repouso) e à suplementação de ácido ascórbico (SU = suplementados - 35 mg/kg de peso, NS = não suplementados). Foram constatadas as seguintes diferenças significativas (p < 0,05): a) no grupo sedentário, aumento da histamina muscular e cardíaca tanto nos subgrupos não suplementados como nos suplementados, o mesmo ocorrendo entre os animais treinados-exercitados agudamente somente naqueles suplementados; b) menor aumento de histamina muscular nos animais treinados - não suplementados - exercitados agudamente quando comparados a seus respectivos controles e de histamina cardíaca nos animais sedentários - suplementados - exercitados agudamente, quando comparados aos não suplementados, na mesma condição, e c) diminuição da histamina cardíaca nos animais sedentários, em repouso e suplementados

Download Full-text

ANTIOXIDANT STATUS IN VARIOUS PATHOGENIC SUBTYPES OF ISCHEMIC STROKE IN ACUTE PERIOD

Бюллетень Восточно-Сибирского научного центра Сибирского отделения Российской академии медицинских наук ◽

10.12737/23747 ◽

2016 ◽

Vol 1 (6) ◽

pp. 76-81

Author(s):

Лукьянова ◽

Yuliya Lukyanova

Keyword(s):

Ascorbic Acid ◽

Ischemic Stroke ◽

Antioxidant Capacity ◽

Antioxidant Status ◽

Control Group ◽

Blood Levels ◽

Cell Recovery ◽

Stroke Subtype ◽

Acute Period ◽

Group 5

The aim of the study was to detect significant biochemical changes in antioxidant status during acute period of ischemic stroke in its various pathogenic subtypes in patients aged 45–74years. The most important results were blood levels of ascorbic acid, malondialdehyde and free/ oxidized glutathione ratio. Cardioembolic stroke was associated with: the reduced ascorbic acid level of 62–74% comparable to control group; 5-times increased malondialdehyde level at the end of the first week of the disease; and also 50% decreasing of cell antioxidant capacity comparable to control group and other stroke subtypes. In lacunary stroke minimal changes in antioxidant status were measured, possibly because of small size of brain damage. Positive clinical dynamics was followed by decreasing of free glutathione levels. It could be related with its previous significant expenditure in process of cell recovery in penumbra area. In case of favorable outcome all of the markers return to the levels which were comparable to control group. The differences in the dynam-ics of the cells antioxidant capacity and consumption of native antioxidants, the time from the onset of the disease, the severity of the clinical picture, depending on the stroke subtype, were revealed. Further research of this problem may help to create new methods of diagnostics and selective pharmacological correction of this pathology.

Download Full-text

THE DIAGNOSTIC VALUE OF SERUM CREATINE KINASE IN NEUROMUSCULAR AND MUSCULAR DISEASE

PEDIATRICS ◽

10.1542/peds.35.2.322 ◽

1965 ◽

Vol 35 (2) ◽

pp. 322-330

Author(s):

Franco Vassella ◽

Roland Richterich ◽

Ettore Rossi

Keyword(s):

Creatine Kinase ◽

Muscular Dystrophy ◽

Epileptic Seizures ◽

Lactic Dehydrogenase ◽

Diagnostic Value ◽

Creatine Kinase Activity ◽

Serum Creatine Kinase ◽

Progressive Muscular Dystrophy ◽

Muscular Disease ◽

Muscle Specificity

Serum creatine kinase activity in 200 patients was studied in order to check whether this determination might be of help in differential diagnosis between primary and secondary (neurogenic) myopathies. Elevated CK values are found physiologically in children, pregnant women, and after exercise. In pathological states increased serum enzyme concentrations (probably the expression of a defect in cellular permeability) are constantly present in the Duchenne type of progressive muscular dystrophy. More variable values are encountered in other types of muscular dystrophy and in metabolic disorders like Refsum's syndrome, coproporphyria, and hypothyroidism. In states of necrosis or injury to muscle cells like in polymyositis, dermatomyositis, after burns or trauma, serum CK concentrations vary according partly to the amount of muscle mass involved. Enzyme concentrations can be markedly increased after tetany or epileptic seizures. Normal values or occasionally slightly increased values are found in neurogenic myopathies. High CK values in patients with muscular atrophies should permit the clinician to exclude the differential diagnostic group of neurogenic myopathies. As a diagnostic tool CK proved to be more reliable than transaminases or lactic dehydrogenase because of its higher muscle specificity and a slightly greater sensitivity.

Download Full-text

Mo1031 PROTECTIVE ROLE OF THE PRO-RESOLVING LIPID MEDIATOR RESOLVIN D1 IN NONSTEROIDAL ANTI-INFLAMMATORY DRUG-INDUCED SMALL INTESTINAL DAMAGE

Gastroenterology ◽

10.1016/s0016-5085(20)32579-8 ◽

2020 ◽

Vol 158 (6) ◽

pp. S-762

Author(s):

Takuya Kuzumoto ◽

Tetsuya Tanigawa ◽

Hiroyuki Kitamura ◽

Akira Higashimori ◽

Yuji Nadatani ◽

...

Keyword(s):

Protective Role ◽

Lipid Mediator ◽

Intestinal Damage ◽

Resolvin D1 ◽

Drug Induced ◽

Inflammatory Drug ◽

Small Intestinal ◽

Anti Inflammatory

Download Full-text

Histological study of the effect of potassium dichromate on the thyroid follicular cells of adult male albino rat and the possible protective role of ascorbic acid (vitamin C)

Journal of Microscopy and Ultrastructure ◽

10.1016/j.jmau.2014.04.003 ◽

2014 ◽

Vol 2 (3) ◽

pp. 137 ◽

Cited By ~ 2

Author(s):

SadikaM Tawfik ◽

RedaH ElBakry

Keyword(s):

Ascorbic Acid ◽

Vitamin C ◽

Adult Male ◽

Potassium Dichromate ◽

Histological Study ◽

Protective Role ◽

Follicular Cells ◽

Albino Rat ◽

Thyroid Follicular Cells

Download Full-text

EFFECT OF PHYSICAL EXERCISE ON COGNITION IN ADULT DAY PARTICIPANTS

Innovation in Aging ◽

10.1093/geroni/igz038.2421 ◽

2019 ◽

Vol 3 (Supplement_1) ◽

pp. S652-S653

Author(s):

Bradford Stucki ◽

Ben Katz ◽

Jonathan Briganti ◽

Ila Schepisi ◽

Shannon Jarrott

Keyword(s):

Older Adults ◽

Physical Exercise ◽

Effective Means ◽

Cognitive Status ◽

Data Set ◽

Moderation Effect ◽

Exercise Interventions ◽

Mini Mental Status Exam ◽

Exercise Regimen ◽

Exercise Activity

Abstract Aerobic exercise has been demonstrated to be one of the most effective means of improving cognition in older adults. However, less is known about how exercise programs may improve cognition in older adults participating in Adult Day Service (ADS) programs. We analyzed a ten-year longitudinal data set from the Virginia Tech ADS center. We limited our analyses to individuals for whom we had two time points of the Mini-Mental Status Exam (MMSE) (n=142; average age = 78.48; 63 female, average days at center = 347; SD=432.71). Participants in the center completed approximately 30 minutes of physical exercise each day of attendance. The exercise regimen was largely composed of aerobic chair exercise, stretching, and lifting. Facilitator ratings of engagement with the exercise activity between the two test administrations were used to create an average engagement score for each participant. Multiple regression analyses were conducted using engagement as a predictor and change in MMSE as an outcome; no significant relationship was identified between exercise engagement and change in cognitive status. However, a moderation analysis conducted with diagnosis of Alzheimer’s disease (AD) or dementia as a predictor, change in MMSE as an outcome, and exercise engagement as a moderator revealed a significant moderation effect (p = .001). Greater exercise engagement was associated with improvements on the MMSE, but only for individuals without a diagnosis of AD or dementia. Given that many ADS programs serve individuals both with and without AD or dementia, these findings may inform more personalized exercise interventions at ADS centers.

Download Full-text