Enzymatic Hydrolysis and Fermentation of Banana Pseudostem Hydrolysate to Produce Bioethanol

Banana pseudostem (BPS) is an agricultural waste with a high holocellulose content, which, upon hydrolysis, releases fermentable sugars that can be used for bioethanol production. Different pretreatment methods, namely, 3% (w/v) NaOH, 5% (v/v) H2SO4, and liquid hot water, applied on the BPS resulted in the availability of 52%, 48%, and 25% cellulose after treatment, respectively. Saccharification of the pretreated BPS with 10 FPU/g dry solids (29.3 mg protein/g d.s) crude enzyme from Trichoderma harzianum LMLBP07 13-5 at 50°C and a substrate loading of 10 to 15% released 3.8 to 21.8 g/L and from T. longibrachiatum LMLSAUL 14-1 released 5.4 to 43.5 g/L glucose to the biomass. Ethanol was produced through separate hydrolysis and fermentation (SHF) of alkaline pretreated BPS hydrolysate using Saccharomyces cerevisiae UL01 at 30°C and 100 rpm. Highest ethanol produced was 17.6 g/L. Banana pseudostem was shown as a potentially cheap substrate for bioethanol production.

Download Full-text

Changes in poplar (Populus trichocarpa) wood porous structure after liquid hot water (LHW) pretreatment

Annals of WULS, Forestry and Wood Technology ◽

10.5604/01.3001.0014.8861 ◽

2020 ◽

Vol 112 ◽

pp. 71-78

Author(s):

Florentyna Akus-Szyblerg ◽

Jan Szadkowski ◽

Andrzej Antczak ◽

Janusz Zawadzki

Keyword(s):

Enzymatic Hydrolysis ◽

Porous Structure ◽

Populus Trichocarpa ◽

Hot Water ◽

Size Exclusion ◽

Bioethanol Production ◽

Poplar Wood ◽

Liquid Hot Water ◽

Exclusion Chromatography ◽

Pretreatment Conditions

Changes in poplar (Populus trichocarpa) wood porous structure after liquid hot water (LHW) pretreatment. The aim of this research was to investigate the effect of applying different hydrothermal pretreatment conditions on the porous structure of poplar wood. Porosity is recognised as an important factor considering efficiency of an enzymatic hydrolysis as a step of bioethanol production. Native poplar wood as well as solid fractions after pretreatment performed at different temperatures (160 °C, 175 °C and 190 °C) were analysed. Porous structure was examined with an inverse size-exclusion chromatography (ISEC) method. Results indicated a significant development of the porous structure of the biomass with increasing porosity along with the growing temperature of the LHW process. The temperature of 190 °C was chosen as the most promising condition of poplar wood LHW pretreatment in terms of the efficiency of the subsequent steps of bioethanol production. The obtained results were consistent with the previous experimental data procured during analysis of the LHW pretreated poplar wood and its subsequent enzymatic hydrolysis yield.

Download Full-text

Pressurized liquid hot water pretreatment and enzymatic hydrolysis of switchgrass aiming at the enhancement of fermentable sugars

Biofuels ◽

10.1080/17597269.2019.1706273 ◽

2020 ◽

pp. 1-6

Author(s):

Kaniye Gunes ◽

Mine Yaglikci ◽

Melih Soner Celiktas

Keyword(s):

Enzymatic Hydrolysis ◽

Hot Water ◽

Fermentable Sugars ◽

Liquid Hot Water Pretreatment ◽

Liquid Hot Water ◽

Water Pretreatment ◽

Hot Water Pretreatment ◽

Hydrolysis Of

Download Full-text

A Comparison of the Bioethanol Production from Suweg (Amorphophallus campanulatus) through Separate Hydrolysis and Fermentation as well as Simultaneous Saccharification and Fermentation Using Saccharomyces cerevisiae

IOP Conference Series Materials Science and Engineering ◽

10.1088/1757-899x/1053/1/012036 ◽

2021 ◽

Vol 1053 (1) ◽

pp. 012036

Author(s):

H Hargono ◽

B Jos ◽

P Purwanto ◽

S Sumardiono ◽

MF Zakaria

Keyword(s):

Saccharomyces Cerevisiae ◽

Simultaneous Saccharification And Fermentation ◽

Bioethanol Production ◽

Separate Hydrolysis And Fermentation ◽

Simultaneous Saccharification

Download Full-text

Diverse Banana Pseudostems and Rachis Are Distinctive for Edible Carbohydrates and Lignocellulose Saccharification towards High Bioethanol Production under Chemical and Liquid Hot Water Pretreatments

Molecules ◽

10.3390/molecules26133870 ◽

2021 ◽

Vol 26 (13) ◽

pp. 3870

Author(s):

Jingyang Li ◽

Fei Liu ◽

Hua Yu ◽

Yuqi Li ◽

Shiguang Zhou ◽

...

Keyword(s):

Soluble Sugars ◽

Enzymatic Saccharification ◽

Hot Water ◽

Biofuel Production ◽

Bioenergy Crops ◽

Bioethanol Production ◽

Factors Affecting ◽

Liquid Hot Water ◽

Biomass Processing ◽

Banana Crops

Banana is a major fruit crop throughout the world with abundant lignocellulose in the pseudostem and rachis residues for biofuel production. In this study, we collected a total of 11 pseudostems and rachis samples that were originally derived from different genetic types and ecological locations of banana crops and then examined largely varied edible carbohydrates (soluble sugars, starch) and lignocellulose compositions. By performing chemical (H2SO4, NaOH) and liquid hot water (LHW) pretreatments, we also found a remarkable variation in biomass enzymatic saccharification and bioethanol production among all banana samples examined. Consequently, this study identified a desirable banana (Refen1, subgroup Pisang Awak) crop containing large amounts of edible carbohydrates and completely digestible lignocellulose, which could be combined to achieve the highest bioethanol yields of 31–38% (% dry matter), compared with previously reported ones in other bioenergy crops. Chemical analysis further indicated that the cellulose CrI and lignin G-monomer should be two major recalcitrant factors affecting biomass enzymatic saccharification in banana pseudostems and rachis. Therefore, this study not only examined rich edible carbohydrates for food in the banana pseudostems but also detected digestible lignocellulose for bioethanol production in rachis tissue, providing a strategy applicable for genetic breeding and biomass processing in banana crops.

Download Full-text

Liquid Hot Water Pretreatment and Enzymatic Hydrolysis as a Valorization Route of Italian Green Pepper Waste to Delivery Free Sugars

Foods ◽

10.3390/foods9111640 ◽

2020 ◽

Vol 9 (11) ◽

pp. 1640

Author(s):

M.A. Martín-Lara ◽

L. Chica-Redecillas ◽

A. Pérez ◽

G. Blázquez ◽

G. Garcia-Garcia ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Liquid Fraction ◽

Hot Water ◽

Effect Of Temperature ◽

Green Pepper ◽

Free Sugars ◽

Liquid Hot Water Pretreatment ◽

Liquid Hot Water ◽

Water Pretreatment ◽

Hot Water Pretreatment

In this work, liquid hot water pretreatment (autohydrolysis) was used to improve enzymatic hydrolysis of a commonly consumed vegetable waste in Spain, Italian green pepper, to finally produce fermentable sugars. Firstly, the effect of temperature and contact time on sugar recovery during pretreatment (in insoluble solid and liquid fraction) was studied in detail. Then, enzymatic hydrolysis using commercial cellulase was performed with the insoluble solid resulting from pretreatment. The objective was to compare results with and without pretreatment. The results showed that the pretreatment step was effective to facilitate the sugars release in enzymatic hydrolysis, increasing the global sugar yield. This was especially notable when pretreatment was carried out at 180 °C for 40 min for glucose yields. In these conditions a global glucose yield of 61.02% was obtained. In addition, very low concentrations of phenolic compounds (ranging from 69.12 to 82.24 mg/L) were found in the liquid fraction from enzymatic hydrolysis, decreasing the possibility of fermentation inhibition produced by these components. Results showed that Italian green pepper is an interesting feedstock to obtain free sugars and prevent the enormous quantity of this food waste discarded annually.

Download Full-text