scholarly journals Soxhlet Extraction versus Hydrodistillation Using the Clevenger Apparatus: A Comparative Study on the Extraction of a Volatile Compound from Tamarindus indica Seeds

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Kehinde Oluwakemi Fagbemi ◽  
Daniel Ayandiran Aina ◽  
Olufunmiso Olusola Olajuyigbe
Keyword(s):  
Methyl Ester ◽  
Essential Oil ◽  
Volatile Compound ◽  
Octadecadienoic Acid ◽  
Vaccenic Acid ◽  
Soxhlet Extraction ◽  
Chemical Compounds ◽  
Tamarindus Indica ◽  
Hexadecanoic Acid ◽  
Oil Components

The present study aims to compare two traditional extraction techniques. A volatile compound from Tamarindus indica seed was obtained with Soxhlet extraction (SE) and hydrodistillation using the Clevenger apparatus (HDC). The extraction yield and chemical composition of the essential oil samples were compared. Both oils extracted were analyzed with GC-MS, and forty-one chemical compounds were identified in essential oil components from SE while forty chemical compounds were found in the HDC-extracted oil sample. The major essential oil components present in both the SE and HDC method are cis-vaccenic acid, 2-methyltetracosane, beta-sitosterol, 9,12-octadecadienoic acid (Z, Z)-, and n-hexadecanoic acid in varying concentrations. Moreover, the essential oils obtained by both methods look similar quantitatively but differ qualitatively. The HDC method produced more oxygenated compounds that contribute to the fragrance of the oil. The major constituents observed in the essential oil extracted by SE methods include cis-vaccenic acid (17.6%), beta-sitosterol (12.71%), 9,12-octadecadienoic acid (Z, Z)- (11.82%), n-hexadecanoic acid (8.16%), 9,12-octadecadienoic acid, methyl ester (5.84%), oleic acid (4.54%), and 11-octadecenoic acid and methyl ester (3.94%). However, in the hydrodistillation technique, the oil was mostly composed of 9,12-octadecadienoic acid (Z, Z)- (23.72%), cis-vaccenic acid (17.16%), n-hexadecanoic acid (11.53%), beta-sitosterol (4.53%), and octadecanoic acid (3.8%). From the data obtained, HDC seems to be a better method for extraction of Tamarindus indica essential oil compared to the Soxhlet extraction apparatus.

Chemical Composition of the Essential Oil of Elephantopus scaber from Southern China

2004 ◽  
Vol 59 (5-6) ◽  
pp. 327-329 ◽  
Author(s):  
Li Wanga ◽  
Shuguang Jian ◽  
Peng Nan ◽  
Yang Zhong
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Octadecadienoic Acid ◽  
Southern China ◽  
Medicinal Herb ◽  
Hexadecanoic Acid ◽  
Elephantopus Scaber ◽  
First Time

The essential oil of Elephantopus scaber, a famous medicinal herb from Yangjiang County in Southern China was analyzed for the first time using GC-MS. The major constituents were hexadecanoic acid (42.3%), isopropyl dimethyl tetrahydronaphthalenol (14.1%), β-sesquiphellandrene (8.3%), octadecadienoic acid (5.5%), and phytol (5.2%).

scholarly journals Profil GCMS Senyawa Kimia Ekstrak Metanol Isolat Cendawan Entomopatogen Beauveria Bassiana Dan Akar Cabai Sebagai Pemacu Pertumbuhan Cabai

2020 ◽  
Vol 4 (2) ◽  
pp. 106-118
Author(s):  
Magdalena Saragih ◽  
Trizelia Trizelia ◽  
Nurbailis Nurbailis ◽  
Yusniwati Yusniwati
Keyword(s):  
Acetic Acid ◽  
Methyl Ester ◽  
Palmitic Acid ◽  
Chemical Compound ◽  
Entomopathogenic Fungus ◽  
Acid Methyl Ester ◽  
Chemical Compounds ◽  
Hexadecanoic Acid ◽  
Ethanoic Acid ◽  
Acid Methyl

The aim of this study was to identify the chemical compound methanol extract of entomopathogenic fungus Beauveria bassiana from insect walang sangit and the chemical compound roots of red chili plants that were able to stimulate the growth of chilli plants after being applied with entomopathogenic fungus   B. bassiana through seed immersion inoculation using GCMS method. The chemical compound identified as a growth booster in   B. bassiana fungus isolates from the insect walang sangit is an Acetic acid Ethanoic acid Ethylic acid Glacial acetic acid CH3COOH, Hexadecanoic acid, methyl ester (CAS) Methylpalmitate Uniphat A60, n-Hexadecanoic acid Hexadecanoic acid n-Hexadecoic acid Palmitic acid, Dianhydromannitol and Ergosta-5,7,22-trien-3-ol, (3.beta., 22E) - (CAS) Ergosterol (CAS), while the chili root contains Acetic acid Ethanoic acid Ethylic acid Glacials acetic acid CH3COOH, Hexadecanoic acid, methyl ester (CAS) Methyl palmitate Uniphat A60, n -Hexadecanoic acid Hexadecanoic acid Palmitic acid, 8, 11- octadecadienoic acid, methyl ester (CAS) METHYL 8, 11-OCTADECADIENOATE, (23S)-ethylcholest-5-en-3.beta.-ol.s It can be concluded that some of the chemical compounds in methanol extracts identified in chili roots have similarities with chemical compounds that exist in   B. bassiana entomopathogenic fungus which are potential as stimulers of chili plant growth

scholarly journals GC-MS ANALYSIS PROFILE AND BIOACTIVE COMPONENTS OF FLOWERS OF Bergenia ciliata, A WEED OF ROCK CREVICES

2021 ◽  
Vol 27 (4) ◽  
pp. 527-535
Author(s):  
Malik F. H. Ferdosi ◽  
Iqra Haider Khan ◽  
Arshad Javaid ◽  
Hafiz M. Saeed ◽  
Ifrayeem Butt ◽  
...  
Keyword(s):  
Methyl Ester ◽  
Octadecadienoic Acid ◽  
Acid Methyl Ester ◽  
Literature Survey ◽  
Pentadecanoic Acid ◽  
Bioactive Components ◽  
Hexadecanoic Acid ◽  
Acid Methyl ◽  
Ms Analysis ◽  
Hexanedioic Acid

Bergenia ciliata is a weed of hilly areas of Pakistan that generally grows in rock crevices. In the present study, flowers of this weeds were collected from Murree. The dried flowers were extracted in methanol and subjected to GC-MS analysis that showed 7 compounds in it. The predominant compound was hexanedioic acid, bis(2-ethylhexyl) ester (48.88%) followed by γ-sitosterol (22.56%). Moderately occurring compound was cyclohexane, 1,3,5-triphenyl- (12.87%). The remaining compounds namely n-hexadecanoic acid (4.97%), pentadecanoic acid, 14-methyl-, methyl ester (3.77%), 9,12-octadecadienoic acid, methyl ester, (Z,Z)-  (2.94%) and 9,12,15-octadecatrienoic acid, methyl ester, (Z,Z,Z)- (2.92%) were categorized as less abundant compounds. Literature survey indicated that these compounds possess antimicrobial, larvicidal, anti-inflammatory, anticancer and/or antidiabetic properties.

scholarly journals BIOCHEMICAL PROFILE OF Calotropis procera FLOWERS

2021 ◽  
Vol 27 (3) ◽  
pp. 341-349
Author(s):  
Malik Fiaz Hussain Ferdosi ◽  
Iqra Haider Khan ◽  
Arshad Javaid ◽  
Ayesha Munir
Keyword(s):  
Methyl Ester ◽  
Octadecadienoic Acid ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Calotropis Procera ◽  
Biochemical Profile ◽  
Hexadecanoic Acid ◽  
Flower Extract ◽  
Acid Methyl ◽  
Peak Areas

Calotropis procera is a medicinal weed of family Asclepiadaceae. This study was carried out to explore the biochemical profile of C. procera flowers collected from Southern Punjab region of Pakistan. Methanolic flower extract of C. procera was subjected to GC-MS analysis. There were 30 compounds identified in this extract. The predominant compound was γ-sitosterol with 15.39% peak area. Other abundantly occurring compounds included stigmasterol (9.22%), 9,12-octadecadienoic acid (Z,Z)-, methyl ester (9.01%), campesterol (8.63%), α-amyrin acetate (8.25%), β-amyrin (8.09%), hexadecanoic acid, methyl ester (7.91%), 11-octadecenoic acid, methyl ester (6.15%), and 2-methoxy-4-vinylphenol (5.66%). Moderately abundant compounds included nonacos-1-ene (2.83%), methyl stearate (1.57%), pentacosane (1.44%), phytol (1.33%), heptacos-1-ene (1.20%), heneicosane (1.19%), and 1-hexacosene (1.09%). The remaining less abundant compounds were present with peak areas less than 1%. Literature survey showed that the major compounds identified in the flower extract of C. procera possess various bioactivities including antidiabetic, anticancer, antihyperglycemic, antioxidant, antimicrobial and anti-inflamatory.

scholarly journals GC and GC/MS Analysis of the Essential Oil of Salvia hierosolymitana Boiss. Growing Wild in Lebanon

2007 ◽  
Vol 2 (2) ◽  
pp. 1934578X0700200 ◽  
Author(s):  
Carmen Formisano ◽  
Felice Senatore ◽  
Nelly Apostolides Arnold ◽  
Franco Piozzi ◽  
Sergio Rosselli
Keyword(s):  
Essential Oil ◽  
Octadecadienoic Acid ◽  
Hexadecanoic Acid ◽  
Aerial Parts ◽  
Ms Analysis ◽  
Hexahydrofarnesyl Acetone

The essential oil of the aerial parts of Salvia hierosolymitana Boiss. (Lamiaceae), growing wild in Lebanon, was obtained by hydrodistillation and analysed by GC and GC-MS. Ninety-two compounds, representing 92.7% of the oil, were identified. The major components were hexadecanoic acid (15.5%), phytol (5.4%), hexahydrofarnesyl acetone (4.6%), (Z,Z)-9,12-octadecadienoic acid (4.5%) and 4-vinylguaiacol (4.4%).

Cyclamen persicum: Methanolic Extract Using Gas Chromatography-Mass Spectrometry (GC-MS) Technique

2017 ◽  
Vol 8 (04) ◽  
Author(s):  
Israa Adnan Ibraheam ◽  
Imad Hadi Hameed S. ◽  
Haider Mashkoor Hussein
Keyword(s):  
Methyl Ester ◽  
Phthalic Acid ◽  
Methanolic Extract ◽  
Octadecadienoic Acid ◽  
Acid Methyl Ester ◽  
Chemical Compounds ◽  
Gas Chromatography Mass Spectrometry ◽  
Molecular Formula ◽  
Cyclamen Persicum ◽  
The Family

Cyclamen was traditionally classified in the family Primulaceae, was reclassified in the subfamily Myrsinoideae within the family Primulaceae. The objective of this study was analysis of the secondary metabolite products. Bioactives are chemical compounds often referred to as secondary metabolites. Thirty eight bioactive compounds were identified in the methanolic extract of Cyclamen persicum. The identification of bioactive chemical compounds is based on the peak area, retention time molecular weight and molecular formula. GC-MS analysis of Cyclamen persicum revealed the existence of the3-Oxo-androsta-1,4-dien-17β-spiro-2'-3'-oxo-oxetane, 3,5-Dithiahexanol 5,5-dioxide, 1-(2-Nitrophenyl)piperazine, Oxime-,methoxy-phenyl- , Cyclohexene, 1-methyl-4-(1-methylethenyl)-,(S)-, D-Limonene , Fumaric acid,3-methylbut-3-enyl undecyl ester, Geranyl vinyl ether, 3,6,9,12-Tetraoxatetradecan -1-ol,14-[4-(1-,1,3,3-tetramethylbu, Cis-5,8,11,14,17-Eicosapentaenoic acid, α-Terpineol, 3-Allyl-6-methoxyphenol, 3-Cyclohexene-1-methanol,α,α,4-trimethyl-,acetate, Orcinol, 4,5-di-epi-aristolochene, Trans-calamenene, 3-(N,N-Dimethyllaurylammonio)propanesulfonate, Deoxyqinghaosu, Atranorin , N-[4-(4-Chlorophenyl)isothiazol-5-yl)-1-methylpiperidin-2-imine, 10-Heptadecen-8-ynoic acid , methyl ester, (E)-, 2-Pentadecanone,6,10,14-trimethyl-, Caffeine, 4,4,8-Trimethyltricyclo[6.3.1.0(1.5)]dodecane-2,9-diol , Bufa-20,22-dienolide, 3,14-dihydroxy-,(3β,5β)-, 1-(3-methyl-2-butenyl)-3,6-diazahomoadamantan-9-ol , 9,12-Octadecadienoic acid (Z,Z)-, methyl ester, 9-Octadecenamide,(Z)-, 9,10-Secocholesta -5,7,10(19)-triene-3,24,25-triol,(3β,5Z,7E)-, Tributyl acetylcitrate, Cyproheptadine, 3,9-Epoxypregn-16-en-20-one , 3-methoxy-7,11,18-triacetoxy- , 17-Pentatriacontene, Phthalic acid , bis(7-methyloctyl) ester, Phthalic acid, di(6-ethyl-3-octyl) ester, Ergosterol, γ-Sitosterol and Friedelan-3-one.

scholarly journals Chemical Composition of the Essential Oil of the Local Endemics Centaurea davidovii and C. parilica (Asteraceae, sect. Lepteranthus) from Bulgaria

2014 ◽  
Vol 9 (9) ◽  
pp. 1934578X1400900
Author(s):  
Antonella Maggio ◽  
Luana Riccobono ◽  
Svetlana Bancheva ◽  
Maurizio Bruno ◽  
Felice Senatore
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Octadecadienoic Acid ◽  
Chemical Compositions ◽  
Caryophyllene Oxide ◽  
Pca Analysis ◽  
Hexadecanoic Acid ◽  
Aerial Parts ◽  
Main Components

In the present study the chemical compositions of the essential oils from aerial parts of Centaurea davidovii Urum. and C. parilica Stoj. & Stef., both endemic to Bulgaria, were evaluated by GC and GC-MS. The main components of C. davidovii were β-eudesmol (13.9%), spathulenol (13.3%), caryophyllene oxide (10.1%) and ( Z)-phytol (5.4%). The main components of C parilica were hexadecanoic acid (39.2%), ( Z, Z)-9,12-octadecadienoic acid (11.9%), caryophyllene oxide (6.8%) and spathulenol (6.6%). In order to compare the essential oils composition of these taxa and of related species a PCA analysis was carried out.

scholarly journals Simultaneous Determination of Essential Oil Components and Fatty Acids in Fennel using Gas Chromatography with a Polar Capillary Column

2015 ◽  
Vol 10 (9) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Menče Najdoska-Bogdanov ◽  
Jane B. Bogdanov ◽  
Marina Stefova
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Essential Oil ◽  
Simultaneous Determination ◽  
Soxhlet Extraction ◽  
Volatile Fraction ◽  
Foeniculum Vulgare ◽  
Oil Components ◽  
Main Components

Cultivated and wild growing samples of fennel ( Foeniculum vulgare Mill., Apiaceae) from R. Macedonia were studied for their volatiles and fatty acid composition. The main essential oil components isolated via hydrodistillation were: trans-anethole (>80%), estragole (<6%), limonene (<6%), anisaldehyde (<1%) and 0.5 % fenchone. An alternative method for characterization of both the non-polar volatile and non volatile fractions was developed using n-hexane and dichloromethane (3:1, v/v) in a Soxhlet extraction followed by transesterification. The obtained extracts were then characterized and the dominant fatty acid was 18:1 (petroselinic and oleic acid) 75.0–82.8 %, followed by 18:2 (linoleic acid) 10.8–16.2% and other fatty acids: palmitic (4.3–6.9%), stearic (1.2–1.7%) and myristic (0–2.9%). The results for the volatile fraction after Soxhlet extraction and transesterification did not significantly differ from results obtained after hydrodistillation, especially for the main components ( trans-anethole, estragole, fenchone and limonene), implying that the developed method can be used for simultaneous determination of volatiles and fatty acids.

Anti-Neuropathic Pain Activity of Ageratum conyzoides L due to The Essential Oil Components

2020 ◽  
Vol 19 ◽  
Author(s):  
Yedy Purwandi Sukmawan ◽  
Kusnandar Anggadiredja ◽  
I Ketut Adnyana
Keyword(s):  
Neuropathic Pain ◽  
Essential Oil ◽  
Opioid Receptor ◽  
Steam Distillation ◽  
Chronic Constriction Injury ◽  
Thermal Hyperalgesia ◽  
Ageratum Conyzoides ◽  
Safety And Efficacy ◽  
Activity Test ◽  
Oil Components

Background: Neuropathic pain is one of the contributors to the global burdens of illness. At present many patients do not achieve satisfactory pain relief even with synthetic pain-killers. Taking this into consideration, it is necessary to search for natural product-derived alternative treatment with confirmed safety and efficacy. Ageratum conyzoides L is a plant often used as analgesic in Indonesia, however, anti-neuropathic pain activity of this plant is still unknown. Objective: To determine the anti-neuropathic pain activity of the essential oil and non-essential oil component (distillation residue) of A. conyzoides L. Methods: We conducted separation of the essential oil component from other secondary metabolites through steam distillation. Both components were tested for anti-neuropathic pain activity using chronic constriction injury animal models with thermal hyperalgesia and allodynia tests. The animals were divided into 7 test groups namely normal, sham, negative, positive (pregabalin at 0.195 mg/20 g BW of mice), essential oil component (100 mg/kg BW), and non-essential oil component (100 mg/kg BW). Naloxone was tested against the most potent anti-neuropathic pain component (essential oil or nonessential oil) to investigate the involvement of opioid receptor. Results: The GC-MS of the essential oil component indicated the presence of 60 compounds. Meanwhile, non-essential oil components contained alkaloid, flavonoid, polyphenol, quinone, steroid, and triterpenoid. This non-essential oil component contained a total flavonoid equivalent to 248.89 ppm quercetin. The anti-neuropathic pain activity test showed significantly higher activity of the essential oil component compared to the non-essential oil component and negative groups (p<0.05). Furthermore, the essential oil component showed equal activity to pregabalin (p>0.05). However, this activity was abolished by naloxone, indicating the involvement of opioid receptor in the action of the essential oil component. Conclusion: The essential oil component of A. conyzoides L is a potential novel substance for use as anti-neuropathic pain.

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