scholarly journals Ascorbic Acid and Polyphenols Mediated Green Synthesis of Silver Nanoparticles from Tagetes erecta L. Aqueous Leaf Extract and Studied Their Antioxidant Properties

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Pankaj Kumar Tyagi ◽  
Shruti Tyagi ◽  
Deepak Gola ◽  
Arvind Arya ◽  
Seyed Abdulmajid Ayatollahi ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Free Radical ◽  
Leaf Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Tagetes Erecta ◽  
Aqueous Leaf Extract ◽  
Tagetes Erecta L ◽  
Phenolic And Flavonoid

Silver nanoparticle synthesis of the leaf extract Tagetes erecta L. enriched with ascorbic acid and polyphenols has been investigated. The color of the golden yellow extract has changed to pinkish-brown due to the reduction of Ag+ to the colloidal solution of AgNPs and a sharp absorption peak at 420 nm under the UV-Vis spectrophotometer. In addition, the Fourier Transfer Infrared Spectroscopy (FTIR) estimation was completed in order to recognize and identify the biomolecules present in the extract acting as a reducing and capping agent for the AgNPs. The X-ray diffraction (XRD) peaks at (111), (201), (220), and (311) confirm the presence of monoclinic crystals in the solution. The morphology and size of the particles were provided by transmission electron microscopy (TEM) images of AgNPs. At a scale of 100 nm, synthesized AgNPs were predominantly spherical with a size range of 7-35 nm. In comparison to 7.39 mg/100 g in AgNPs, aqueous leaf extract was 55.14 mg/100 g higher in ascorbic acid. The phenolic and flavonoid content of extract was 52.54 ± 2.15  mg (GAE/100 g) and 15.43 ± 0.34  mg (QE/mL), and the colloidal AgNP solution was 21.45 ± 1.15  mg (GAE/100 g) and 8.05 ± 2.42  mg (QE/mL), respectively. Phenolic and flavonoid contents play a major role as a reducing agent and reduce the precursor AgNO3 into AgNPs. The DPPH scavenging assay also assessed the antioxidant properties of extract and its derived AgNPs. As compared antioxidant value to aqueous leaf extract (mg/mL), higher percentage inhibition (PI) was found in AgNPs and free-radical scavenging activity of extract and AgNPs were directly linked to their concentrations. Results of this research have discovered a higher potential for free-radical scavenging AgNPs and will help to develop new and more potent antioxidants for the treatment of different diseases caused by oxidative stress; the higher antioxidant properties bearing AgNPs might be used.

scholarly journals Antioxidant and antimicrobial activities of methanol leaf extract of Aegle Tamilnadensis Abdul Kader

2017 ◽  
Vol 9 (4) ◽  
pp. 648
Author(s):  
M Chella Kumar ◽  
S Abdul Kader ◽  
C Sivaraj ◽  
N Raaman
Keyword(s):  
Free Radical ◽  
Leaf Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antimicrobial Activities ◽  
Free Radical Scavenging ◽  
Diffusion Method ◽  
Antioxidant And Antimicrobial Activities ◽  
Radical Scavenging Assay

The present investigation was aimed to evaluate the phytochemical constituents, free radical scavenging activities, antioxidant properties and antimicrobial activities of the methanol leaf extract of <em>Aegle tamilnadensis </em>Abdul Kader. The phytochemicals present in the methanol leaf extract of <em>A. tamilnadensis</em> Abdul Kader<em> </em>were determined qualitatively and quantitatively using standard procedures. The antioxidant activities were carried out by DPPH free radical scavenging assay, OH<sup>•</sup> radical scavenging assay, NO<sup>• </sup>radical scavenging assay, Fe<sup>3+ </sup>reducing power assay, and phosphomolybdenum reduction assay methods. The antimicrobial activity was carried out by well diffusion method. The methanol leaf extract of <em>A. tamilnadensis</em> Abdul Kader<em> </em>showed good free radical scavenging as well as reducing power activities which were found to increase with the increasing concentration of the extract. The study revealed the presence of major phytochemicals such as phenols and flavonoids at the concentration of 211.0 mg/g at 100 µg and 52.91 mg/g at 100µg/mL respectively. The present study revealed that the methanol leaf extract of <em>A. tamilnadensis</em> Abdul Kader<em> </em>possess significant antioxidant and antimicrobial activities.

scholarly journals Biological activities and phytochemical screening of Elaeodendron croceum (Thunb.) DC. Leaves and Stem barks Extracts

2017 ◽  
Vol 9 (4) ◽  
pp. 566 ◽  
Author(s):  
Samuel Wale Odeyemi ◽  
Anthony Jide Afolayan
Keyword(s):  
Antibacterial Activity ◽  
Free Radical ◽  
Leaf Extract ◽  
Protein Denaturation ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Diffusion Method ◽  
Aqueous Leaf Extract ◽  
Anti Inflammatory

<p><strong>Background:</strong> From reports <em>Elaeodendron croceum</em> has been used traditionally for spiritual cleansing, anti-obesity, anti-inflammatory and for the management of diabetes. The leaves and bark extracts were therefore analyzed for its biological activities.</p><p><strong>Materials and methods:</strong> The antibacterial activities of <em>E. croceum</em> leaves and stem barks were evaluated against both Gram positive and gram negative bacteria using agar well diffusion method. The analysis for the phytochemical contents, antioxidant and anti-inflammatory activities of the leaves and barks were determined using standard procedures.</p><p><strong>Results:</strong> The <em>S. aureus</em>, <em>S. flexneri</em>, <em>E. faecalis</em> and <em>S. typhimurium</em> were susceptible to the aqueous leaves and stem barks extracts of <em>Elaeodendron croceum</em> while <em>S. aureus </em>was susceptible to only the aqueous leaf extract. The antibacterial activity showed MIC ranging from 0.625 – 2.5 mg/ml. The composition of flavonoids (52.72 mg/g), proanthocyanidins (32.66 mg/g), alkaloids (65.44 mg/g) and saponins (68.21 mg/g) were significantly higher in the aqueous leaf extract while phenols (94.13 mg/g) content was higher in the aqueous bark extract. The free radical scavenging activity using ABTS, FRAP and DPPH showed that the extracts possess good free radical scavenging activities with different free radical species that is comparable with ascorbic acid and also showed significant inhibitory activity on protein denaturation as an indication of anti-inflammatory potential.</p><strong>Conclusion:</strong> These findings support the folkloric usage of <em>Elaeodendron croceum</em> and indicates that it can be used to manage diseases caused by the tested organisms. The presence of alkaloids, flavonoids, total phenols and proanthocyanidins, previously suggested to have antioxidant, anti-inflammatory and antibacterial activity indicate that <em>E. croceum</em> is a potential source of anti-inflammatory, free radical scavenging and antimicrobial agents.

Flavones and Their Analogues as Bioactive Compounds – An Overview

2019 ◽  
Vol 16 (4) ◽  
pp. 377-391 ◽  
Author(s):  
B.S. Jayashree ◽  
H. Venkatachalam ◽  
Sanchari Basu Mallik
Keyword(s):  
Biological Activity ◽  
Free Radical ◽  
Bioactive Compounds ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Cost Effective ◽  
Free Radical Scavenging ◽  
Polyphenolic Compounds ◽  
Pharmacological Activities ◽  
Poor Solubility

Flavonoids constitute a large group of polyphenolic compounds that are known to have antioxidant properties, through their free radical scavenging abilities. They possess a chromone (γ- benzopyrone) moiety, responsible for eliciting many pharmacological activities. Even though, natural flavonoids are highly potent, owing to their poor solubility, they are less used. Therefore, attempts have been made to improve their stability, solubility, efficacy and kinetics by introducing various substituents on the flavone ring. For nearly the last two decades, flavones were synthesized in our laboratory by simple, convenient and cost-effective methods, with the knowledge of both synthetic and semi-synthetic chemistry. In this direction, it was considered worthwhile to present an overview on the synthesized flavonoids. This review creates a platform for highlighting various modifications done on the flavone system along with their biological activity.

scholarly journals In Vitro and In Vivo Antioxidant Properties of Taraxacum officinale in Nω-Nitro-l-Arginine Methyl Ester (L-NAME)-Induced Hypertensive Rats

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 309
Author(s):  
Olukayode O. Aremu ◽  
Adebola O. Oyedeji ◽  
Opeoluwa O. Oyedeji ◽  
Benedicta N. Nkeh-Chungag ◽  
Constance R. Sewani Rusike
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Methyl Ester ◽  
Leaf Extract ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Hypertensive Rats ◽  
Total Antioxidant

Oxidative stress has gained attention as one of the fundamental mechanisms responsible for the development of hypertension. The present study investigated in vitro and in vivo antioxidant effects of 70% ethanol-water (v/v) leaf and root extracts of T. officinale (TOL and TOR, respectively). Total phenolic and flavonoid content of plant extracts were assessed using Folin Ciocalteau and aluminium chloride colorimetric methods; while, 2,2-diphenyl-1-picrlhydrazyl (DPPH), 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and ferric reducing antioxidant power (FRAP) protocols were used to determine the free radical scavenging and total antioxidant capacities (TAC), respectively. The in vivo total antioxidant capacity and malondialdehyde acid (MDA) levels for lipid peroxidation tests were performed on organ homogenate samples from Nω-nitro-L-arginine methyl ester (L-NAME)-induced hypertensive rats treated with leaf extract, TOL (500 mg/kg/day) and TOR (500 mg/kg/day) for 21 days. Results showed that compared to TOR, TOL possessed significantly higher (p < 0.01) polyphenol (4.35 ± 0.15 compared to 1.14 ± 0.01) and flavonoid (23.17 ± 0.14 compared to 3 ± 0.05) content; free radical scavenging activity (EC50 0.37 compared to 1.34 mg/mL) and total antioxidant capacities (82.56% compared to 61.54% ABTS, and 156 ± 5.28 compared to 40 ± 0.31 FRAP) and both extracts showed no toxicity (LD50 > 5000 mg/kg). TOL and TOR significantly (p < 0.01) elevated TAC and reduced MDA levels in targets organs. In conclusion, T. officinale leaf extract possesses significant anti-oxidant effects which conferred significant in vivo antioxidant protection against free radical-mediated oxidative stress in L-NAME-induced hypertensive rats.

scholarly journals Evaluation of Antioxidant Properties ofPhaulopsis fascisepalaC.B.Cl. (Acanthaceae)

2009 ◽  
Vol 6 (2) ◽  
pp. 227-231 ◽  
Author(s):  
S. A. Adesegun ◽  
A. Fajana ◽  
C. I. Orabueze ◽  
H. A. B. Coker
Keyword(s):  
Ascorbic Acid ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Chelating Ability

The antioxidant activities of crude extract ofPhaulopsis fascisepalaleaf were evaluated and compared with α-tocopherol and BHT as synthetic antioxidants and ascorbic acid as natural-based antioxidant.In vitro, we studied its antioxidative activities, radical-scavenging effects, Fe2+-chelating ability and reducing power. The total phenolic content was determined and expressed in gallic acid equivalent. The extract showed variable activities in all of thesein vitrotests. The antioxidant effect ofP. fascisepalawas strongly dose dependent, increased with increasing leaf extract dose and then leveled off with further increase in extract dose. Compared to other antioxidants used in the study, α-Tocopherol, ascorbic acid and BHT,P. fascisepalaleaf extract showed less scavenging effect on α,α,-diphenyl-β-picrylhydrazyl (DPPH) radical and less reducing power on Fe3+/ferricyanide complex but better Fe2+-chelating ability. These results revealed thein vitroantioxidant activity ofP.fascisepala.Further investigations are necessary to verify these activitiesin vivo.

scholarly journals Antioxidant and antimicrobial activities against Enterobacter cloacae of extracts of Eclipta alba Hassk.

2016 ◽  
Vol 19 (4) ◽  
pp. 76-83
Author(s):  
Trang Thi Xuan Dai ◽  
Anh Thi Tu Vo
Keyword(s):  
Free Radical ◽  
Vitamin C ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Enterobacter Cloacae ◽  
Free Radical Scavenging ◽  
Antioxidant Vitamin ◽  
Eclipta Alba

This study was subjected to investigate antimicrobial and antioxidant properties of Eclipta alba extracts (methanol, hexane, chloroform and ethyl acetate extracts). The antioxidant property of the extract was assessed by 2,2- diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method. Kirby-Bauer method was used to determine the antibacterial activity against Enterobacter cloacae of the extracts. The extracts of Eclipta alba were tested against E. cloacae. The highest antibacterial potentiality was exhibited by the ethyl acetate extract of E. alba leaves, means of zones of bacterial growth inhibition are 26.3 mm at a concentration of 32 μg/mL. DPPH free radical scavenging effect of the extracts was compared with standard antioxidant vitamin C. The highest antioxidant activity was exhibited by the ethyl acetate extract of E. alba leaves. The result also showed that the DPPH scavenging activity of ethyl acetate extract from leaves of E. alba was high (EC50 = 419.38 μg/mL). However, this result was 18.99 times lower than that of vitamin C (EC50 = 22.08 μg/mL).

Free radical scavenging and antioxidant properties of conducting polymers examined using EPR and NMR spectroscopies

2005 ◽  
Vol 153 (1-3) ◽  
pp. 153-156 ◽  
Author(s):  
P.A. Kilmartin ◽  
M. Gizdavic-Nikolaidis ◽  
Z. Zujovic ◽  
J. Travas-Sejdic ◽  
G.A. Bowmaker ◽  
...  
Keyword(s):  
Free Radical ◽  
Conducting Polymers ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Free Radical Scavenging

scholarly journals The Free Radical Scavenging and Anti-Isolated Human LDL Oxidation Activities of Pluchea indica (L.) Less. Tea Compared to Green Tea (Camellia sinensis)

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Kittipot Sirichaiwetchakoon ◽  
Gordon Matthew Lowe ◽  
Griangsak Eumkeb
Keyword(s):  
Free Radical ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Hypochlorous Acid ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Ldl Oxidation ◽  
Caffeoylquinic Acid ◽  
Dicaffeoylquinic Acid

Tea is one of the most popular beverages in the world. Camellia sinensis tea (CST) or green tea is widely regarded as a potent antioxidant. In Thailand, Pluchea indica (L.) Less. tea (PIT) has been commercially available as a health-promoting drink. This study focused on free radical scavenging activities of PIT, and its ability to protect isolated human low-density lipoproteins (LDL) from oxidation by chemical agents. A preliminary study to investigate the antioxidant nature of PIT was undertaken. These included common antioxidant assays involving 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,2-azinobis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS), hypochlorous acid (HOCl), and its potential to scavenge peroxynitrite. In separated experiments, isolated human LDL was challenged with either 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH), copper (Cu2+), or 3-Morpholinosydnonimine hydrochloride (SIN-1) to induce LDL oxidation. PIT exhibited antioxidant activity in all test systems and performed significantly better than CST in both DPPH (P<0.05; IC50PIT=245.85±15.83 and CST=315.41±24.18 μg/ml) and peroxynitrite scavenging assays. PIT at 75 μg/ml almost fully prevented the peroxynitrite over a 5 h period. Moreover, it displayed similar properties to CST during the antioxidation of isolated human LDL using AAPH, Cu2+, SIN-1, and hypochlorous acid scavenging assays. However, it revealed a significantly lower ABTS scavenging activity than CST (P<0.05; IC50PIT=30.47±2.20 and CST=21.59±0.67 μg/ml). The main constituents of the PIT were identified using LC-MS/MS. It contained 4-O-caffeoylquinic acid (4-CQ), 5-O-caffeoylquinic acid (5-CQ), 3,4-O-dicaffeoylquinic acid (3,4-CQ), 3,5-O-dicaffeoylquinic acid (3,5-CQ), and 4,5-O-dicaffeoylquinic acid (4,5-CQ). In conclusion, caffeoyl derivatives in PIT could play an important role in potent antioxidant properties. So, it may be further developed to be antioxidant beverages for preventing atherosclerosis and cardiovascular diseases associated with oxidative stress.

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