scholarly journals Antioxidant and antimicrobial activities against Enterobacter cloacae of extracts of Eclipta alba Hassk.

2016 ◽  
Vol 19 (4) ◽  
pp. 76-83
Author(s):  
Trang Thi Xuan Dai ◽  
Anh Thi Tu Vo
Keyword(s):  
Free Radical ◽  
Vitamin C ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Enterobacter Cloacae ◽  
Free Radical Scavenging ◽  
Antioxidant Vitamin ◽  
Eclipta Alba

This study was subjected to investigate antimicrobial and antioxidant properties of Eclipta alba extracts (methanol, hexane, chloroform and ethyl acetate extracts). The antioxidant property of the extract was assessed by 2,2- diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method. Kirby-Bauer method was used to determine the antibacterial activity against Enterobacter cloacae of the extracts. The extracts of Eclipta alba were tested against E. cloacae. The highest antibacterial potentiality was exhibited by the ethyl acetate extract of E. alba leaves, means of zones of bacterial growth inhibition are 26.3 mm at a concentration of 32 μg/mL. DPPH free radical scavenging effect of the extracts was compared with standard antioxidant vitamin C. The highest antioxidant activity was exhibited by the ethyl acetate extract of E. alba leaves. The result also showed that the DPPH scavenging activity of ethyl acetate extract from leaves of E. alba was high (EC50 = 419.38 μg/mL). However, this result was 18.99 times lower than that of vitamin C (EC50 = 22.08 μg/mL).

scholarly journals ASSESSMENT OF ANTIOXIDANT ACTIVITIES, PHENOL AND FLAVONOID CONTENTS OF DIFFERENT EXTRACTS OF LEAVES, BARK AND ROOT FROM THE Abutilon indicum (L.) SWEET

2017 ◽  
Vol 10 (4) ◽  
pp. 88
Author(s):  
Adaikalam Raj ◽  
Saranya D ◽  
Sekar J
Keyword(s):  
Free Radical ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenol ◽  
Butylated Hydroxytoluene ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Abutilon Indicum

Objective: This study evaluated the antioxidant properties of petroleum ether, chloroform, ethyl acetate and methanol extracts of Abutilon indicum (L).Methods: The different extracts of A. indicum leaves, bark and roots  were antioxidant potential by using 1,1-diphenyl-2-picryl-hydrazyl (DPPH), 2, 2-Azino-bis-3-ethyl benzthiazoline-6-sulphonic acid (ABTS•+) , Hydrogen peroxide scavenging, Superoxide anions scavenging,  Hydroxyl radical scavenging, Ferric reducing antioxidant power, Total antioxidant activity (Phosphomolybdic acid) and total phenol and flavonoid contents.                      Results: The highest total phenol and flavonoid contents in leaves extracts of A. indicum. The total phenol (3.08 ± 0.06) mg/ml and flavonoid (7.16 ± 0.15) mg/ml were found to be higher in ethyl acetate extract of A. indicum and free radical scavenging activities IC50 values were calculated and compare to standard for L- ascorbic acid, (BHT) butylated hydroxytoluene and gallic acid    Conclusion:  The results of the study revealed that the ethyl acetate extract of A. indicum leaves can be used for the biological characterization and importance of the compounds identified and creates a platform to screen many bioactive compounds to treat many diseases.Keywords: Abutilon indicum, Free radical scavenging activity, 1,1-diphenyl-2-picryl-hydrazyl, 2-azino-bis-3-ethyl benzthiazoline-6-sulfonic acid,Ferric reducing antioxidant power.

scholarly journals GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS, IN VITRO CYTOTOXIC AND ANTIOXIDANT EFFICACY STUDIES ON CLEOME GYNANDRA L. (LEAVES): A TRADITIONAL DRUG SOURCE

2017 ◽  
Vol 10 (10) ◽  
pp. 84
Author(s):  
Renuka Saravanan ◽  
Brindha Pemaiah ◽  
Mahesh Narayanan ◽  
Sivakumar Ramalingam
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Cleome Gynandra ◽  
Mcf 7

  Objective: This study was aimed to assess the phytoconstituents, cytotoxic, and antioxidant efficacy of ethyl extract of Cleome gynandra leaves.Methods: Qualitative phytochemical analysis with different solvent extracts was performed. Quantitative and gas chromatography-mass spectrometry (GC-MS) analysis of the extract was performed with ethyl acetate extract. The cytotoxic effect of the ethyl acetate extract was determined by 3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide (MTT) assay on Michigan Cancer Foundation-7 (MCF-7) cells using taxol as standard and free radical scavenging ability using 1,1-diphenyl-2-picrylhydrazyl (DPPH).Results: Leaves extracts with different solvents revealed the presence of alkaloids, cardiac glycosides, flavonoids, phenols, and tannins. GC-MS analysis of ethyl acetate of the plant leaves showed the presence of n-hexadecanoic acid. The IC50 value of the ethyl acetate extract was found to be 90.2 μg/ml on MCF-7 cell line, and the extract was found to possess significant DPPH free radical scavenging activity.Conclusion: From the results, we conclude that the C. gynandra extract possesses antioxidant and antiproliferating activity against MCF-7 cells.

scholarly journals Biological activity studies of Sensevieria hyacinthoides extracts

2020 ◽  
Vol 5 (1) ◽  
pp. 15-20
Author(s):  
Mohammad Emdadul Islam ◽  
Tofail Ahmed Chowdhury ◽  
Nasim Sultana ◽  
Abul Hasnat Muhammad Solaiman ◽  
SM Anamul Arefin
Keyword(s):  
Biological Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity

Biological activity was studied of the different solvent extracts such as n-hexane, ethyl acetate, n-butanol, and methanol extracts of the rhizomes of Sensevieria hyacinthoides. The cytotoxic potential were examined by using brine shrimp lethality bioassay. Ethyl acetate extract exhibited quite potent activity in brine shrimp lethality bioassay with LC50 1.95 μg/mL, respectively. These result suggested that it might contain antitumor or cytotoxic agent. The ethyl acetate extract showed significant free radical scavenging activity with IC50 10.51 μg/mL and demonstrated excellent antibacterial activity. The free radical scavenging activity of the solvent extracts (n-hexane, ethyl acetate and n-butanol) were assayed by using DPPH method. The IC50 for Vit-c (Ascorbic acid) and ethyl acetate extract were found to be 3.91 and 10.51 μg/mL, respectively. Asian Australas. J. Biosci. Biotechnol. 2020, 5 (1), 15-20

scholarly journals PHYTOCHEMICAL, GAS CHROMATOGRAPHY-MASS SPECTROMETRY PROFILING AND FREE RADICAL SCAVENGING ACTIVITY OF EXOCARPOS LONGIFOLIUS (SANTALACEAE) EXTRACTS

2018 ◽  
Vol 11 (10) ◽  
pp. 452
Author(s):  
Praptiwi Praptiwi ◽  
Ahmad Fathoni
Keyword(s):  
Free Radical ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Phytochemical Screening

Objective: The objectives of the study were to investigate phytochemical screening and gas chromatography-mass spectrometry (GC-MS) profiling of twigs and leaves of Exocarpos longifolius and its 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity.Methods: Phytochemical screening covering saponins, alkaloids, terpenoids, flavonoids, and tannins were carried out by standard methods. The free radical scavenging activity was performed by thin-layer chromatography - bioautography, followed by determining the IC50 values of active extracts. Chemical compounds of active extract were examined by gas chromatography/mass spectrometry.Results: The phytochemical screening revealed that all extracts have been shown to contain tannins and terpenoids, saponins were found in the methanol extract of twigs, and alkaloids were found in the ethyl acetate extracts of twigs and leaves, while flavonoids were found in the methanol extract and ethyl acetate extracts of twigs and leaves of E. longifolius. Ethyl acetate extract of twigs showed the best of IC50 value (15.65 μg/mL), while ethyl acetate extract of leaves and methanol extract of twigs have strong free radical scavenging activity which IC50 were 78.59 and 67.24 μg/mL, respectively. The GC-MS analysis of ethyl acetate extract of twigs has shown the presence of 12 identified compounds, and the main chemical compound is Stigmast-4-en-3-one (21.91%).Conclusions: The ethyl acetate extract of E. longifolius showed a very powerful free radical scavenging activity and revealed chemical compounds had been known to have biological activity. It might be used as a natural antioxidant.

scholarly journals Oxygen radical antioxidant capacity (ORAC) and antibacterial properties of Melicope glabra bark extracts and isolated compounds

PLoS ONE ◽  
2021 ◽  
Vol 16 (5) ◽  
pp. e0251534
Author(s):  
Alexandra Quek ◽  
Hafizah Mohd Zaini ◽  
Nur Kartinee Kassim ◽  
Fadzil Sulaiman ◽  
Yaya Rukayadi ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging ◽  
Antibacterial Properties ◽  
Plant Secondary Metabolites ◽  
Oxygen Radical ◽  
Free Radical Scavenging

Melicope glabra (Blume) T. G. Hartley from the Rutaceae family is one of the richest sources of plant secondary metabolites, including coumarins and flavanoids. This study investigates the free radical scavenging and antibacterial activities of M. glabra and its isolated compounds. M. glabra ethyl acetate and methanol extracts were prepared using the cold maceration technique. The isolation of compounds was performed with column chromatography. The free radical scavenging activity of the extracts and isolated compounds were evaluated based on their oxygen radical absorbance capacity (ORAC) activities. The extracts and compounds were also subjected to antibacterial evaluation using bio-autographic and minimal inhibitory concentration (MIC) techniques against two oral pathogens, Enterococcus faecalis and Streptococcus mutans. Isolation of phytoconstituents from ethyl acetate extract successfully yielded quercetin 3, 5, 3’-trimethyl ether (1) and kumatakenin (2), while the isolation of the methanol extract resulted in scoparone (3), 6, 7, 8-trimethoxycoumarin (4), marmesin (5), glabranin (6), umbelliferone (7), scopoletin (8), and sesamin (9). The study is the first to isolate compound (1) from Rutaceae plants, and also the first to report the isolation of compounds (2–5) from M. glabra. The ORAC evaluation showed that the methanol extract is stronger than the ethyl acetate extract, while umbelliferone (7) exhibited the highest ORAC value of 24 965 μmolTE/g followed by glabranin (6), sesamin (9) and scopoletin (8). Ethyl acetate extract showed stronger antibacterial activity towards E. faecalis and S. mutans than the methanol extract with MIC values of 4166.7 ± 1443.4 μg/ml and 8303.3 ± 360.8 μg/ml respectively. Ethyl acetate extract inhibited E. faecalis growth, as shown by the lowest optical density value of 0.046 at a concentration of 5.0 mg/mL with a percentage inhibition of 95%. Among the isolated compounds tested, umbelliferone (7) and sesamin (9) exhibited promising antibacterial activity against S. mutans with both exhibiting MIC values of 208.3 ± 90.6 μg/ml. Findings from this study suggests M. glabra as a natural source of potent antioxidant and antibacterial agents.

Flavones and Their Analogues as Bioactive Compounds – An Overview

2019 ◽  
Vol 16 (4) ◽  
pp. 377-391 ◽  
Author(s):  
B.S. Jayashree ◽  
H. Venkatachalam ◽  
Sanchari Basu Mallik
Keyword(s):  
Biological Activity ◽  
Free Radical ◽  
Bioactive Compounds ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Cost Effective ◽  
Free Radical Scavenging ◽  
Polyphenolic Compounds ◽  
Pharmacological Activities ◽  
Poor Solubility

Flavonoids constitute a large group of polyphenolic compounds that are known to have antioxidant properties, through their free radical scavenging abilities. They possess a chromone (γ- benzopyrone) moiety, responsible for eliciting many pharmacological activities. Even though, natural flavonoids are highly potent, owing to their poor solubility, they are less used. Therefore, attempts have been made to improve their stability, solubility, efficacy and kinetics by introducing various substituents on the flavone ring. For nearly the last two decades, flavones were synthesized in our laboratory by simple, convenient and cost-effective methods, with the knowledge of both synthetic and semi-synthetic chemistry. In this direction, it was considered worthwhile to present an overview on the synthesized flavonoids. This review creates a platform for highlighting various modifications done on the flavone system along with their biological activity.

scholarly journals EVALUATION OF ANTIOXIDANT AND FREE RADICAL SCAVENGING ACTIVITIES OF DIFFERENT SOLVENT EXTRACTS OF LEAVES OF PIPER UMBELLATUM

2017 ◽  
Vol 10 (2) ◽  
pp. 274
Author(s):  
Sethupandian Geetha ◽  
Kokkaiah Irulandi ◽  
Palanichamy Mehalingam
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Frap Assay ◽  
Radical Scavenging Properties

Objective: This study was designed to determine the Total phenol, flavonoid content, antioxidant and free radical scavenging properties of different solvent extracts of Piper umbellatum.  Methods: Different solvent extracts evaluated with DPPH radical scavenging activity and Reducing power activity.Results: The presence of phenol and flavonoid showed highly in the methanol extracts than ethyl acetate and acetone extracts. All the extracts have various level of antioxidant activity. Methanol solvent extract have good extraction and show significant antioxidant activity. The effect of reducing power of methanol extract revealed good antioxidant activity compare with other tested extracts.Conclusion: On the basis of the above results we concluded that methanol extract of Piper umbellatum whole plant extracts shows significant antioxidant activity than ethyl acetate and acetone extracts.Keywords: Piper umbellatum, Antioxidant, DPPH, FRAP assay, Polar, Non polar solvents

Free radical scavenging and antioxidant properties of conducting polymers examined using EPR and NMR spectroscopies

2005 ◽  
Vol 153 (1-3) ◽  
pp. 153-156 ◽  
Author(s):  
P.A. Kilmartin ◽  
M. Gizdavic-Nikolaidis ◽  
Z. Zujovic ◽  
J. Travas-Sejdic ◽  
G.A. Bowmaker ◽  
...  
Keyword(s):  
Free Radical ◽  
Conducting Polymers ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Free Radical Scavenging

scholarly journals The Free Radical Scavenging and Anti-Isolated Human LDL Oxidation Activities of Pluchea indica (L.) Less. Tea Compared to Green Tea (Camellia sinensis)

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Kittipot Sirichaiwetchakoon ◽  
Gordon Matthew Lowe ◽  
Griangsak Eumkeb
Keyword(s):  
Free Radical ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Hypochlorous Acid ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Ldl Oxidation ◽  
Caffeoylquinic Acid ◽  
Dicaffeoylquinic Acid

Tea is one of the most popular beverages in the world. Camellia sinensis tea (CST) or green tea is widely regarded as a potent antioxidant. In Thailand, Pluchea indica (L.) Less. tea (PIT) has been commercially available as a health-promoting drink. This study focused on free radical scavenging activities of PIT, and its ability to protect isolated human low-density lipoproteins (LDL) from oxidation by chemical agents. A preliminary study to investigate the antioxidant nature of PIT was undertaken. These included common antioxidant assays involving 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,2-azinobis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS), hypochlorous acid (HOCl), and its potential to scavenge peroxynitrite. In separated experiments, isolated human LDL was challenged with either 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH), copper (Cu2+), or 3-Morpholinosydnonimine hydrochloride (SIN-1) to induce LDL oxidation. PIT exhibited antioxidant activity in all test systems and performed significantly better than CST in both DPPH (P<0.05; IC50PIT=245.85±15.83 and CST=315.41±24.18 μg/ml) and peroxynitrite scavenging assays. PIT at 75 μg/ml almost fully prevented the peroxynitrite over a 5 h period. Moreover, it displayed similar properties to CST during the antioxidation of isolated human LDL using AAPH, Cu2+, SIN-1, and hypochlorous acid scavenging assays. However, it revealed a significantly lower ABTS scavenging activity than CST (P<0.05; IC50PIT=30.47±2.20 and CST=21.59±0.67 μg/ml). The main constituents of the PIT were identified using LC-MS/MS. It contained 4-O-caffeoylquinic acid (4-CQ), 5-O-caffeoylquinic acid (5-CQ), 3,4-O-dicaffeoylquinic acid (3,4-CQ), 3,5-O-dicaffeoylquinic acid (3,5-CQ), and 4,5-O-dicaffeoylquinic acid (4,5-CQ). In conclusion, caffeoyl derivatives in PIT could play an important role in potent antioxidant properties. So, it may be further developed to be antioxidant beverages for preventing atherosclerosis and cardiovascular diseases associated with oxidative stress.

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