scholarly journals Expression of E-Cadherin in Pig-Tailed Monkey (Macaca nemestrina) Endometrium after Controlled Ovarian Hyperstimulation

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Nurhuda Sahar ◽  
R. Muharam ◽  
Andhea Debby Pradhita ◽  
Rosalina Thuffi ◽  
Wa Ode Zulhulaifah ◽  
...  
Keyword(s):  
Success Rate ◽  
Steroid Hormones ◽  
Implantation Rate ◽  
Controlled Ovarian Hyperstimulation ◽  
Macaca Nemestrina ◽  
Control Group ◽  
Ovarian Hyperstimulation ◽  
Cycle Group ◽  
Rate Research ◽  
E Cadherin

An increase of steroid hormones in controlled ovarian hyperstimulation (COH) procedures is reducing the success rate in assisted reproductive technology (ART), and this includes the pregnancy rate and/or implantation rate. Research has found that the decrease in the success rate occurred due to the decreased expression of the protein that is needed to prepare the endometrium so that the embryo could attach. The aim of the study was to analyse the changes in E-chaderin expression due to COH and its relations with increased level of steroid hormones as one of the proteins in the endometrium. There were 13 samples of stored biological tissue from Macaca nemestrina endometrial tissue; came from one group of natural cycles as the control group ( n = 4 ) and three groups of stimulated cycles. The first stimulated cycle group was injected by a 30 IU dose of rFSH ( n = 2 ). The second stimulated cycle group was injected by a 50 IU dose of rFSH ( n = 4 ). The third stimulated cycle group was injected by a 70 IU dose of rFSH ( n = 3 ). The expression of E-cadherin was measured by the immunohistochemistry (IHC) technique. Estradiol (E2) and progesterone (P4) levels were assessed using ELISA and have already been done. The IHC staining expression of E-cadherin was found in the cytoplasm of glandular epithelium. Immunostaining measurement used the H_SCORE. We found that the expression of E-cadherin within the group was not significantly different ( p value: 0.178). Similarly, both the correlation between the estradiol level with E-cadherin and the correlation between the progesterone level with E-cadherin were not significantly different ( p value: 0.872 and p value: 0.836). The conclusion is that the level of E-Cadherin expression in the endometrium that were taken in themiddle secretion phase not affected by the dose regimen that given. In addition, the level of expression is not influenced by the increase of serum E2 and P4 levels.

scholarly journals Increased Progesterone on the Day of Administration of hCG in Controlled Ovarian Hyperstimulation Affects the Expression of HOXA10 in Primates’ Endometrial Receptivity

Biomedicines ◽  
2019 ◽  
Vol 7 (4) ◽  
pp. 83
Author(s):  
Nurhuda Sahar ◽  
Ninik Mujihartini ◽  
Dwi Ari Pudjianto ◽  
Adhea Debby Pradhita ◽  
Rosalina Thuffi ◽  
...  
Keyword(s):  
Controlled Ovarian Hyperstimulation ◽  
Follicular Phase ◽  
Endometrial Receptivity ◽  
Macaca Nemestrina ◽  
Control Group ◽  
Ovarian Hyperstimulation ◽  
High Concentration ◽  
Natural Group ◽  
Negative Effect ◽  
Long Protocol

The increase in progesterone (P4) levels on the day of human chorionic gonadotropin (hCG) administration have a negative effect on endometrial receptivity. There are few reports regarding the expression of homeobox A10 (HOXA10) as one of many biomolecular factors of endometrial receptivity. To evaluate the effect of increased P4 concentration on the day of hCG administration on HOXA10, a total of 16 Macaca nemestrina were divided into three dose groups of recombinant-follicle stimulating hormone (rFSH) (30IU, 50IU, and 70IU) and one control group. Injection of rFSH combined with gonadotropin release hormone (GnRH) at 160 ug/day was given subcutaneously using a long protocol technique. Blood samples for estradiol (E2) and (P4) concentration measurements were taken on the day of injecting hCG in the final follicular phase, while the collection of endometrial tissue for HOXA10 measurement was carried out 8 to 10 days after hCG administration. E2 and P4 were measured by ELISA, whereas HOXA10 expression was measured with immunohistochemical (IHC) techniques. The concentration of E2 and P4 was found to be higher in dose groups compared with the natural group, but no significant differences were found within the group. For the Hscore for HOXA10 expression, no significant differences within dose groups were found. In addition, no significant differences for the Hscore for HOXA10 were found when compared to E2 groups. Significantly, the Hscore of HOXA10 was found to be >1 ng/mL in the P4 group compared with the Hscore HOXA10 in the P4 natural group (p = 0.022). The high concentration of P4 caused by ovarian hyperstimulation in the follicular phase stimulates the expression of HOXA10 in the secretion phase.

scholarly journals A study on outcome of controlled ovarian hyperstimulation and intrauterine insemination (husband) in a medical college in Kolkata

2022 ◽  
Vol 13 (1) ◽  
pp. 136-141
Author(s):  
Rajib Roy ◽  
Agniv Sarkar ◽  
Bibhas Saha Dalal
Keyword(s):  
Success Rate ◽  
Ovulation Induction ◽  
Positive Impact ◽  
Endometrial Thickness ◽  
Intrauterine Insemination ◽  
Controlled Ovarian Hyperstimulation ◽  
Infertile Couple ◽  
P Value ◽  
Ovarian Hyperstimulation ◽  
Resource Setting

Background: A combination of controlled ovarian hyperstimulation and intrauterine insemination (IUI) remains an important treatment option for couple having infertility. Success rate of IUI with ovulation induction ranges from 8-20% depending on many factors. Aims and Objectives: To assess the factors affecting the success rate of IUI and to evaluate the success of ovulation Induction by different methods of controlled stimulation protocol. Materials and Methods: It is a duration-based prospective cross-sectional study where total of 67 couples were included by inclusion and exclusion criteria. They underwent 90 cycles of IUI with each couple having a maximum of three cycles. Ovulation induction was done by clomiphene citrate or letrozole or gonadotrophins. Semen preparation was done by density gradient method. The outcomewas measured by positive urine pregnancy test. Range, percentage, confidence interval, mean with standard deviation, median, range, and P-value were calculated. P<0.05 was taken as statistically significant. Results: Out of 90 IUI cycles 8 were successful resulting in a success rate of 8.8% per cycle and 11.9% per couple. Factors that had a positive impact were follicle size >21 sqmm, endometrial thickness >9 mm, post wash count >15 million/ml, >2 cycles of IUI and on the number of follicles 2 or more on the day of trigger. Conclusion: The study concluded that IUI after ovulation induction can be a simple and safe cost-effective procedure in selected group of infertile couple. Clinical significance IUI following ovulation induction can be a successful approach for specific indications in a low-resource setting where options for other ART interventions are absent or limited.

scholarly journals Controlled Ovarian Hyperstimulation Protocol in Infertile Patients During the COVID-19 Pandemic

2021 ◽  
Vol 12 ◽  
Author(s):  
Fei Li ◽  
HuiXia Zhang ◽  
WeiYi Shi ◽  
YiFang Wu ◽  
Ye Tian ◽  
...  
Keyword(s):  
Endometrial Thickness ◽  
Management Strategies ◽  
Implantation Rate ◽  
Controlled Ovarian Hyperstimulation ◽  
Ovarian Hyperstimulation ◽  
Down Regulation ◽  
Cycle Number ◽  
Full Dose ◽  
Infertility Patients

Objectives: To explore the appropriate controlled ovarian hyperstimulation (COH) protocols in infertility patients who received the in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) treatments during the COVID-19 pandemic.Materials and Methods: This retrospective cohort study evaluated the efficiency of the early follicular-phase long-acting GnRH-agonist long (EFLL) protocol (a new protocol developed by Chinese clinicians), prolonged pituitary down-regulation of EFLL protocol (Pro-EFLL), and the GnRH-ant protocol for couples meeting the study criteria between February 2020 and June 2020 who were treated by the First Affiliated Hospital of Zhengzhou University during the COVID-19 pandemic, and compared the pregnancy rates and miscarriage rates per fresh transfer cycle, number of retrieved oocytes, endometrial thickness on the day of hCG injection and the number of fertilized oocytes, mature oocytes, fertilized oocytes, and transferable embryos among the three protocols.Results: We found that the prolonged pituitary down-regulation during the COVID-19 pandemic by utilizing a full-dose of GnRH-a administrated in infertility patients were no differences in clinical outcomes than other protocols, The prolonged pituitary down-regulation protocol and EFLL protocol were associated with a higher Endometrial thickness on the day of hCG injection (12.67 ± 2.21 vs. 12.09 ± 2.35 vs. 10.79 ± 2.38, P &lt; 0.001), retrieved oocytes (14.49 ± 6.30 vs. 15.02 ± 7.93 vs. 10.06 ± 7.63, P &lt; 0.001), mature oocytes (11.60 ± 5.71 vs. 11.96 ± 6.00 vs. 7.63 ± 6.50, P &lt; 0.001), fertilized oocytes (9.14 ± 5.43 vs. 8.44 ± 5.34 vs. 5.42 ± 5.20, P &lt; 0.001), and transferable embryos (4.87 ± 2.96 vs. 6.47 ± 5.12 vs. 3.00 ± 3.28 vs. P &lt; 0.001) in the GnRH-antagonist protocol.Conclusion: We recommend that patients start Gn injections 33–42 days after a pituitary downregulated full dose (3.75 mg) of gonadotropin-releasing hormone agonist during the COVID-19 pandemic, even a delay of 2–4 weeks does not affect the implantation rate. The study can provide a more detailed estimate and clinical management strategies for infertile couples during the COVID-19 pandemic.

scholarly journals Pregnancy Rate after Controlled Ovarian Hyperstimulation and Intrauterine Insemination for the Treatment of Endometriosis following Surgery

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Attila Keresztúri ◽  
Zoltan Kozinszky ◽  
József Daru ◽  
Norbert Pásztor ◽  
János Sikovanyecz ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Outcome Measures ◽  
Live Birth ◽  
Intrauterine Insemination ◽  
Care Center ◽  
Tertiary Care ◽  
Controlled Ovarian Hyperstimulation ◽  
Control Group ◽  
Ovarian Hyperstimulation ◽  
Significant Difference

Objective. To compare pregnancy rate after controlled ovarian hyperstimulation and intrauterine insemination (COH-IUI) with no treatment in patients with endometriosis-associated infertility treated with laparoscopy.Design. A clinical cohort study.Setting. University-level tertiary care center.Patients. 238 women with various stages of endometriosis after laparoscopic treatment.Interventions. Either COH-IUI or follow-up for 12 months.Main Outcome Measures. The primary outcome measures were clinical pregnancy and live birth rate. Predictive factors evaluated were female age, maternal BMI, and duration of infertility.Results. The pregnancy rate attained after the integrated laparoscopy–COH-IUI approach was 53.4%, while it was significantly lower (38.5%) in the control group. Similarly, a significant difference was observed in live births (48.3% versus 34.2%). Patients with severe endometriosis were less likely to achieve pregnancy (38%) and live birth (35%) than their counterparts with milder forms (57% and 53%).Conclusions. In patients with endometriosis-based infertility, surgery followed by COH-IUI is more effective than surgery alone.

Ovarian hyperstimulation affects fluid transporters in the uterus: a potential mechanism in uterine receptivity

2014 ◽  
Vol 26 (7) ◽  
pp. 982 ◽  
Author(s):  
Laura A. Lindsay ◽  
Christopher R. Murphy
Keyword(s):  
Implantation Rate ◽  
Normal Pregnancy ◽  
Controlled Ovarian Hyperstimulation ◽  
Fertility Treatment ◽  
Ovarian Hyperstimulation ◽  
Potential Mechanism ◽  
Uterine Receptivity ◽  
Aquaporin 5 ◽  
Uterine Fluid ◽  
Luminal Epithelial Cells

Controlled ovarian hyperstimulation is commonly used in fertility treatment. Evidence suggests that this could alter the endometrial environment and influence implantation rate. However, the mechanisms underlying this disruption are unknown. A recently developed rat ovarian hyperstimulation (OH) model found alterations in the localisation and expression of several molecules associated with implantation, as well as an increase in luminal fluid at the time of implantation. The present study investigated the effects of OH in rats on the expression of fluid-transporting molecules aquaporin 5 (AQP5) and claudin 4. The expression of these proteins was investigated in uterine luminal epithelial cells of rats undergoing OH and compared with normal pregnancy. There was a significant increase in AQP5 protein in OH rats at the time of implantation, along with a loss of the mesometrial staining gradient, which is thought to contribute to implantation position. At the same time, there was a significant decrease in claudin 4 protein. These results suggest that OH in rats causes a dysregulation in uterine fluid dynamics through modifications to fluid-transporting molecules, resulting in an unfavourable implantation environment for the blastocyst.

Controlled ovarian hyperstimulation: are we monitoring the appropriate sex-steroid hormones?

2008 ◽  
Vol 89 (5) ◽  
pp. 1269-1272 ◽  
Author(s):  
Raoul Orvieto ◽  
Eduard Hod ◽  
Michael Volodarsky ◽  
Roy Homburg ◽  
Jacob Rabinson ◽  
...  
Keyword(s):  
Steroid Hormones ◽  
Controlled Ovarian Hyperstimulation ◽  
Sex Steroid Hormones ◽  
Sex Steroid ◽  
Ovarian Hyperstimulation

scholarly journals Changes in Coagulation and Fibrinolytic Indices in Women with Polycystic Ovarian Syndrome Undergoing Controlled Ovarian Hyperstimulation

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Ying Huang ◽  
Yong Zhao ◽  
Ling Yan ◽  
Yun-Hai Chuai ◽  
Ling-Ling Liu ◽  
...  
Keyword(s):  
Polycystic Ovarian Syndrome ◽  
Controlled Ovarian Hyperstimulation ◽  
Endocrine Function ◽  
Control Group ◽  
Ovarian Hyperstimulation ◽  
Vitro Fertilization ◽  
The Status ◽  
Pcos Group ◽  
Ovarian Syndrome

Background. Polycystic ovarian syndrome (PCOS) women undergoing in vitro fertilization and embryo transfer (IVF-ET) treatment always attain a low cumulative pregnancy rate disaccording with the satisfactory number of oocytes.Objective. We aim to evaluate the status of coagulation and fibrinolytic system in PCOS patients undergoing controlled ovarian hyperstimulation (COH) process.Method. Of the 97 women, 30 patients with PCOS composed the study group; 67 women of child-bearing age with normal endocrine function composed the control group. All participants underwent GnRH agonist standard long protocol, and plasma HCY, FVIII, FX, and D-dimer levels as well as hormone parameters were measured at day of full downregulation, hCG priming, and embryos transfer.Results. On day of full downregulation, FX levels were significantly higher in PCOS group (P<0.01). On hCG priming day, FX and estrogen levels in PCOS group were higher than in the control group and FVIII levels were significantly lower on day of embryos transfer whereas FX and E2 levels were significantly higher in PCOS group.Conclusion. Hypercoagulable state during peri-implantation phase would probably lead to poor microcirculation of endometrium and be one of the most important disadvantages of successful implantation and subsequent clinical pregnancy.

scholarly journals Different Occurrence Rates of Centrally Located Cytoplasmic Granulation in One Cohort Oocytes Show Distinctive Embryo Competence and Clinical Outcomes

2021 ◽  
Author(s):  
Fangyuan Sun ◽  
Jintao Cun ◽  
Rongfang Huang ◽  
Yunyan Chen ◽  
Gideon Verwoerd ◽  
...  
Keyword(s):  
Body Mass Index ◽  
Clinical Outcomes ◽  
Controlled Ovarian Hyperstimulation ◽  
Control Group ◽  
Ovarian Hyperstimulation ◽  
Lower Body ◽  
Blastocyst Formation ◽  
Fresh Embryo Transfer ◽  
Specific Population ◽  
Distinctive Features

Abstract Background Centrally located cytoplasmic granulation (CLCG) is a common cytoplasmic dysmorphism in human oocytes retrieved after controlled ovarian hyperstimulation (COH). This study sought to achieve a better understanding of its formation and effects on clinical outcomes. Methods We retrospectively analyzed the data from 422 intracytoplasmic sperm injection (ICSI) treatment cycles. Three groups of patients were classified according to the rates of CLCG occurrence in one egg cohort, as partial (pCLCG) all (aCLCG) and no CLCG (control). Results The pCLCG group had a significantly lower Body Mass Index (BMI) and higher Anti-Mullerian hormone (AMH) level compared to the control or aCLCG groups. Consistent with these distinctive features in the pCLCG group, fertilization and blastocyst formation rates were reduced significantly in the pCLCG group but not in the aCLCG group. Furthermore, the clinical outcomes in fresh embryo transfer cycles were dramatically reduced in the pCLCG group compared with the control group. However, in frozen/thawed cycles, all three clinical outcomes were significantly reduced in the aCLCG group but not in the pCLCG group. Conclusion We propose that pCLCG may reflect a specific population of patients, and that the CLCG structure is sensitive to cryopreservation.

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