Abstract A226: Cell-free membrane reconstitution system for cis and trans interaction of T-cell co-receptors and ligands

2019 ◽  
Author(s):  
Yunlong Zhao ◽  
Enfu Hui
Keyword(s):  
T Cell ◽  
Membrane Reconstitution ◽  
Free Membrane ◽  
Cis And Trans

scholarly journals Comprehensive analyses of glycolysis-related lncRNAs for ovarian cancer patients

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Jianfeng Zheng ◽  
Jialu Guo ◽  
Linling Zhu ◽  
Ying Zhou ◽  
Jinyi Tong
Keyword(s):  
Ovarian Cancer ◽  
T Cell ◽  
Immune Checkpoint ◽  
Risk Model ◽  
Risk Group ◽  
Risk Groups ◽  
Low Risk ◽  
Model Based ◽  
Potential Biomarker ◽  
Cis And Trans

Abstract Background Not only glycolysis but also lncRNAs play a significant role in the growth, proliferation, invasion and metastasis of of ovarian cancer (OC). However, researches about glycolysis -related lncRNAs (GRLs) remain unclear in OC. Herein, we first constructed a GRL-based risk model for patients with OC. Methods The processed RNA sequencing (RNA-seq) profiles with clinicopathological data were downloaded from TCGA and glycolysis-related genes (GRGs) were obtained from MSigDB. Pearson correlation coefficient between glycolysis-related genes (GRGs) and annotated lncRNAs (|r| > 0.4 and p < 0.05) were calculated to identify GRLs. After screening prognostic GRLs, a risk model based on five GRLs was constructed using Univariate and Cox regression. The identified risk model was validated by two validation sets. Further, the differences in clinicopathology, biological function, hypoxia score, immune microenvironment, immune checkpoint, immune checkpoint blockade, chemotherapy drug sensitivity, N6-methyladenosine (m6A) regulators, and ferroptosis-related genes between risk groups were explored by abundant algorithms. Finally, we established networks based on co-expression, ceRNA, cis and trans interaction. Results A total of 535 GRLs were gained and 35 GRLs with significant prognostic value were identified. The prognostic signature containing five GRLs was constructed and validated and can predict prognosis. The nomogram proved the accuracy of the model for predicting prognosis. After computing hypoxia score of each sample by ssGSEA, we found patients with higher risk scores exhibited higher hypoxia score and high hypoxia score was a risk factor. It was revealed that a total of 21 microenvironment cells (such as Central memory CD4 T cell, Neutrophil, Regulatory T cell and so on) and Stromal score had significant differences between the two groups. Four immune checkpoint genes (CD274, LAG3, VTCN1, and CD47) showed disparate expression levels in the two groups. Besides, 16 m6A regulators and 126 ferroptosis-related genes were expressed higher in the low-risk group. GSEA revealed that the risk groups were associated with tumor-related pathways. The two risk groups were confirmed to be sensitive to several chemotherapeutic agents and patients in the low-risk group were more sensitive to ICB therapy. The networks based on co-expression, ceRNA, cis and trans interaction provided insights into the regulatory mechanisms of GRLs. Conclusions Our identified and validated risk model based on five GRLs is an independent prognostic factor for OC patients. Through comprehensive analyses, findings of our study uncovered potential biomarker and therapeutic target for the risk model based on the GRLs.

scholarly journals A subset of HLA-I peptides are not genomically templated: Evidence for cis- and trans-spliced peptide ligands

2018 ◽  
Vol 3 (28) ◽  
pp. eaar3947 ◽  
Author(s):  
Pouya Faridi ◽  
Chen Li ◽  
Sri H. Ramarathinam ◽  
Julian P. Vivian ◽  
Patricia T. Illing ◽  
...  
Keyword(s):  
T Cell ◽  
Posttranslational Modifications ◽  
Human Leukocyte ◽  
Cell Receptor ◽  
Peptide Fragments ◽  
Leukocyte Antigen ◽  
Cell Immunity ◽  
Probable Outcome ◽  
Hla Binding ◽  
Cis And Trans

The diversity of peptides displayed by class I human leukocyte antigen (HLA) plays an essential role in T cell immunity. The peptide repertoire is extended by various posttranslational modifications, including proteasomal splicing of peptide fragments from distinct regions of an antigen to form nongenomically templated cis-spliced sequences. Previously, it has been suggested that a fraction of the immunopeptidome constitutes such cis-spliced peptides; however, because of computational limitations, it has not been possible to assess whether trans-spliced peptides (i.e., the fusion of peptide segments from distinct antigens) are also bound and presented by HLA molecules, and if so, in what proportion. Here, we have developed and applied a bioinformatic workflow and demonstrated that trans-spliced peptides are presented by HLA-I, and their abundance challenges current models of proteasomal splicing that predict cis-splicing as the most probable outcome. These trans-spliced peptides display canonical HLA-binding sequence features and are as frequently identified as cis-spliced peptides found bound to a number of different HLA-A and HLA-B allotypes. Structural analysis reveals that the junction between spliced peptides is highly solvent exposed and likely to participate in T cell receptor interactions. These results highlight the unanticipated diversity of the immunopeptidome and have important implications for autoimmunity, vaccine design, and immunotherapy.

scholarly journals The Toxoplasma gondii virulence factor ROP16 acts in cis and trans, and suppresses T cell responses

2020 ◽  
Vol 217 (3) ◽  
Author(s):  
Longfei Chen ◽  
David A. Christian ◽  
Joshua A. Kochanowsky ◽  
Anthony T. Phan ◽  
Joseph T. Clark ◽  
...  
Keyword(s):  
T Cell ◽  
Toxoplasma Gondii ◽  
Virulence Factor ◽  
Transcriptional Profiling ◽  
T Cell Responses ◽  
Host Cells ◽  
Cell Responses ◽  
Cis And Trans ◽  
In Cis

The ability of Toxoplasma gondii to inject the rhoptry kinase ROP16 into host cells results in the activation of the transcription factors STAT3 and STAT6, but it is unclear how these events impact infection. Here, parasites that inject Cre-recombinase with rhoptry proteins were used to distinguish infected macrophages from those only injected with parasite proteins. Transcriptional profiling revealed that injection of rhoptry proteins alone was sufficient to induce an M2 phenotype that is dependent on STAT3 and STAT6, but only infected cells displayed reduced expression of genes associated with antimicrobial activity and protective immunity. In vivo, the absence of STAT3 or STAT6 improved parasite control, while the loss of ROP16 resulted in a marked reduction in parasite numbers and heightened parasite-specific T cell responses. Thus, ROP16 is a virulence factor that can act in cis and trans to promote M2 programs and which limits the magnitude of parasite-specific T cell responses.

Electron microscope study of the secretory activity of epithelial cells in embryonic thymus

1990 ◽  
Vol 48 (3) ◽  
pp. 382-383
Author(s):  
H. Alasam
Keyword(s):  
Cell Differentiation ◽  
T Cell ◽  
Epithelial Cells ◽  
Electron Density ◽  
Secretory Activity ◽  
T Cell Differentiation ◽  
High Electron ◽  
Transmission Electron ◽  
Medullary Epithelial Cells ◽  
Thymic Factor

The possibility that intrathymic T-cell differentiation involves stem cell-lymphoid interactions in embryos led us to study the ultrastructure of epithelial cell in normal embryonic thymus. Studies in adult thymus showed that it produces several peptides that induce T-cell differentiation. Several of them have been chemically characterized, such as thymosin α 1, thymopoietin, thymic humoral factor or the serum thymic factor. It was suggested that most of these factors are secreted by populations of A and B-epithelial cells.Embryonic materials were obtained from inbred matings of Swiss Albino mice. Thymuses were disected from embryos 17 days old and prepared for transmission electron microscopy. Our studies showed that embryonic thymus at this stage contains undifferentiated and differentiated epithelial cells, large lymphoblasts, medium and few small lymphocytes (Fig. 5). No differences were found between cortical and medullary epithelial cells, in contrast to the findings of Van Vliet et al,. Epithelial cells were mostly of the A-type with low electron density in both cytoplasm and nucleus. However few B-type with high electron density were also found (Fig. 7).

FAILURE TO DETECT ANTI-HTLV-1 ANTIBODY IN A PATIENT WITH ADULT T-CELL LEUKAEMIA/LYMPHOMA

1998 ◽  
Vol 103 (4) ◽  
pp. 1207-1208 ◽  
Author(s):  
Shan-Shun Luo ◽  
Hideto Tamura ◽  
Norio Yokose ◽  
Kiyoyuki Ogata ◽  
Kazuo Dan
Keyword(s):  
T Cell ◽  
Cell Leukaemia
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