scholarly journals Transforming Growth Factor Alpha Stimulation of Mucosal Tissue Cultures from Head and Neck Squamous Cell Carcinoma Patients Increases Chemoresistance to Cisplatin

Chemotherapy ◽  
2010 ◽  
Vol 56 (4) ◽  
pp. 268-274 ◽  
Author(s):  
Philipp Baumeister ◽  
Maximilian Reiter ◽  
Sabina Schwenk-Zieger ◽  
Ulrich Harréus
Keyword(s):  
Squamous Cell Carcinoma ◽  
Growth Factor ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Alpha ◽  
Mucosal Tissue ◽  
Tissue Cultures ◽  
Factor Alpha ◽  
Stimulation Of

In vitro secretion of transforming growth factor alpha (TGF-α): A comparison of the A431 cell line with three human oesophageal squamous cell carcinoma lines

1992 ◽  
Vol 12 (4) ◽  
pp. 293-302 ◽  
Author(s):  
A. L. Thornley ◽  
G. J. Jones
Keyword(s):  
Squamous Cell Carcinoma ◽  
Growth Factor ◽  
Cell Carcinoma ◽  
Cell Line ◽  
Squamous Cell ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Alpha ◽  
Oesophageal Squamous Cell Carcinoma ◽  
Factor Alpha ◽  
A431 Cell Line

Transforming growth factor alpha (TGF-α) is a single chain polypeptide which exists in a variety of forms differing in molecular weight. These forms are variously present in normal and neoplastic cells. Of particular interest are TGF-α's well-known mitogenic properties. The transition from a normal to a neoplastic cellular state results from signalling defects that may depend upon, iter alia, abonormal levels of expression and secretion of TGF-α. It is known that the secretion of TGF-α may be enhanced appreciably by agents such as phorbol 12-myristate 13-acetate (PMA), serum factors and epidermal growth factor (EGF). Here, we compare the efficacy of these three agents in the elevation of TGF-α secretion in the well studied A431 cell line with their previously undocumented efficacy in certain interesting, but little known, human oesophageal squamous cell carcinoma (SCC) lines.

scholarly journals Expression of Epidermal Growth Factor Receptor and Transforming Growth Factor Alpha in Cancer Bladder: Schistosomal and Non-Schistosomal

2015 ◽  
Vol 9 (4) ◽  
pp. 192-201 ◽  
Author(s):  
Afkar A. Badawy ◽  
Ali El-Hindawi ◽  
Olfat Hammam ◽  
Mona Moussa ◽  
Noha S. Helal ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Bladder Cancer ◽  
Epidermal Growth Factor Receptor ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Growth Factor Receptor ◽  
Transforming Growth Factor Alpha ◽  
Associated Lesions ◽  
Epidermal Growth ◽  
Factor Alpha

Introduction: Overexpression of epidermal growth factor receptor (EGFR) has been described in several solid tumors including bladder cancer. Transforming growth factor alpha (TGFα) is frequently deregulated in neoplastic cells and plays a role in the development of bladder cancer. TGFα-EGFR ligand-receptor combination constitutes an important event in multistep tumorigenesis. Methods: This study was done on 30 bladder biopsies from patients with urothelial carcinoma, 15 with squamous cell carcinoma, 10 with cystitis and 5 normal control bladder specimens. All were immuohistochemically stained with EGFR and TGFα antibodies. Results: EGFR and TGFα were over-expressed in higher grades and late stages of bladder cancer. Moreover, they show higher expression in squamous cell carcinoma compared to urothelial carcinoma and in schistosomal associated lesions than in non-schistosomal associated lesions. Conclusion: EGFR and TGFα could be used as prognostic predictors in early stage and grade of bladder cancer cases, especially those with schistosomal association. In addition they can help in selecting patients who can get benefit from anti-EGFR molecular targeted therapy.

Production of transforming growth factor-alpha by normal rat small intestine

1991 ◽  
Vol 261 (6) ◽  
pp. C994-C1000 ◽  
Author(s):  
J. A. Barnard ◽  
W. H. Polk ◽  
H. L. Moses ◽  
R. J. Coffey
Keyword(s):  
Growth Factor ◽  
Egf Receptor ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Alpha ◽  
Single Class ◽  
Receptor Mrna ◽  
Normal Rat ◽  
Epidermal Growth ◽  
Factor Alpha ◽  
Stimulation Of

Transforming growth factor-alpha (TGF-alpha) and epidermal growth factor (EGF) are similar in structure and biological activity. In the present study, the distributions of TGF-alpha mRNA, TGF-alpha immunoreactivity, and TGF-alpha-EGF receptor mRNA were examined in epithelial and nonepithelial compartments of the jejunum, and the effect of TGF-alpha on growth of a jejunal crypt cell line (IEC-6) was determined. Epithelial cells eluted from the rat jejunal cryptvillus axis expressed TGF-alpha mRNA at twofold higher levels in the villus tip than in the crypt and EGF receptor mRNA at sevenfold higher levels in the villus tip. Expression of these two mRNA transcripts in the subepithelium was low. Immunohistochemical staining showed TGF-alpha immunoreactivity predominantly in the epithelium and muscularis. Immunostaining of villus cells was uniform, whereas crypt cells did not stain. IEC-6 cells bound 125I-EGF to a single class of high-affinity (dissociation constant = 833 pM) receptors. EGF and TGF-alpha (10 ng/ml) only modestly stimulated IEC-6 cell growth in the presence of 5% serum but increased expression of the protooncogenes c-jun and c-myc threefold over control cells. These findings suggest that, among the potential physiological roles for TGF-alpha produced by the jejunal epithelium, promotion of cell migration and modulation of fluid and electrolyte transport may be as relatively important as stimulation of cell proliferation.

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