Wilms Tumor 1 (WT1) mRNA Expression Level at Diagnosis Is a Significant Prognostic Marker in Elderly Patients with Myelodysplastic Syndrome

2016 ◽  
Vol 137 (1) ◽  
pp. 32-39 ◽  
Author(s):  
Joji Nagasaki ◽  
Yasutaka Aoyama ◽  
Masayuki Hino ◽  
Kentaro Ido ◽  
Hiroyoshi Ichihara ◽  
...  
Keyword(s):  
Elderly Patients ◽  
Myelodysplastic Syndrome ◽  
Mrna Expression ◽  
Prognostic Marker ◽  
Wilms Tumor ◽  
High Expression ◽  
Expression Level ◽  
Mrna Expression Level ◽  
Wilms Tumor 1 ◽  
The Relationship

Background/Aims: A high expression of Wilms tumor 1 (WT1) mRNA occurs in most cases of acute leukemia and myelodysplastic syndrome (MDS). Although there are many reports suggesting that acute myeloid leukemia patients with high expression levels of WT1 mRNA have a relatively poor long-term survival, there are few reports addressing the relationship between WT1 levels and prognosis in MDS. Methods: We retrospectively analyzed 42 elderly patients with MDS whose WT1 levels at diagnosis were available, and we assessed the relationships between WT1 levels in peripheral blood and preexisting prognostic factors such as World Health Organization prognostic scores and Revised International Prognostic Scoring System risk categories, bone marrow blast percentages, and chromosomal abnormalities linked to a poor prognosis. We also evaluated the relationship between WT1 levels and prognosis. Results:WT1 levels were significantly different between high- and low-risk MDS patients (p < 0.05). There was a trend towards a significant difference between those with and those without poor prognostic chromosomal rearrangements (p = 0.051). Moreover, the overall survival and progression-free survival were significantly worse in elderly patients with higher levels of WT1 (p = 0.00039 and p = 0.00077, respectively). Conclusions: The WT1 mRNA expression level at diagnosis may be a significant independent prognostic marker for elderly patients with MDS.

scholarly journals Wilms’ tumor 1 mRNA expression: a good tool for differentiating between myelodysplastic syndrome and aplastic anemia in children?

Hematology ◽  
2019 ◽  
Vol 24 (1) ◽  
pp. 480-486
Author(s):  
Yingxi Zuo ◽  
Yifei Cheng ◽  
Leping Zhang ◽  
Yazhen Qin ◽  
Hong Luo
Keyword(s):  
Myelodysplastic Syndrome ◽  
Aplastic Anemia ◽  
Mrna Expression ◽  
Wilms Tumor ◽  
Good Tool ◽  
Wilms Tumor 1

WNT6 mRNA Is Overexpressed in Variuos Non-Hodgkin’s Lymphoma Cases with No Apparent Relation to Cyclin D1 Level.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 4663-4663
Author(s):  
Pawel M. Swoboda ◽  
Monika Gos ◽  
Grzegorz Rymkiewicz ◽  
Katarzyna Blachnio ◽  
Jan Walewski ◽  
...  
Keyword(s):  
Cyclin D1 ◽  
Mrna Expression ◽  
Leukemic Cell ◽  
Expression Level ◽  
Mrna Expression Level ◽  
Prolymphocytic Leukemia ◽  
Lymphoid Malignancies ◽  
Leukemic Cell Lines ◽  
Mantle Cell ◽  
The Relationship

Abstract A casual role for Wnts in the development of some human cancers seems to be established. Binding of Wnt to its receptor results in β-catenin stabilization and in consequence, translocation to the nucleus, where it activates the specific target gene’s expression including CCND1 (cyclin D1). Abnormal levels of β-catenin may therefore contribute to neoplastic transformation by causing accumulation of cyclin D1. Overexpression of cyclin D1, as a result of the t(11;14)(q13;q32) translocation, which places cyclin D1 gene under the enhancer of immunoglobulin heavy chain is considered the hallmark of mantle cell lymphoma (MCL) but high levels of cyclin D1 have also been observed with various levels in some cases of non-MCL lymphoproliferative disorders not involving t(11;14), such as hairy cell leukemia (HCL), splenic lymphoma with villous lymphocytes (SLVL), prolymphocytic leukemia (PLL) and some atypical CLL cases. Little is known about potential Wnt signaling in mature lymphocytes or lymphoid malignancies. Recent finding, that the Wnt pathway regulates the proliferation and survival of normal B lymphocytes, together with WNT16 overexpression in leukemic cell lines and high expression of five other Wnts including WNT6 in CLL suggests that Wnt family members can contribute to tumorgenesis in the immune system. In the current study we evaluated the relationship between Wnts and cyclin D1 mRNA expression level in NHL patients. WNT6 and CCND1 mRNA expression level normalized to 18 sRNA was evaluated by RT-PCR on 200 biopsy samples taken from 150 lymphoma patients. In parallel, samples underwent pathological and flow cytometry examination. WNT6 mRNA was detected in all specimens tested. The 18 sRNA/WNT6 ratio ranged from 1 to 650. Most of the CLL specimens were among those with high WNT6 mRNA expression, but no apparent correlation between the level of WNT6 mRNA expression and cyclin D1 status was found so far.

Glypican-3 mRNA expression level in Wilms tumor: correlation with histological type, stage, and outcome

2017 ◽  
Vol 33 (6) ◽  
pp. 695-703 ◽  
Author(s):  
Md. Nahidul Wari ◽  
Archana George Vallonthaiel ◽  
Aijaz Ahmed ◽  
Deepali Saxena ◽  
Venkateswaran K. Iyer ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Wilms Tumor ◽  
Histological Type ◽  
Expression Level ◽  
Mrna Expression Level

scholarly journals Cytokine and Chemokine mRNA Expressions after Mycobacterium tuberculosis-Specific Antigen Stimulation in Whole Blood from Hemodialysis Patients with Latent Tuberculosis Infection

Diagnostics ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 595
Author(s):  
Ji Young Park ◽  
Sung-Bae Park ◽  
Heechul Park ◽  
Jungho Kim ◽  
Ye Na Kim ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mrna Expression ◽  
Whole Blood ◽  
Latent Tuberculosis Infection ◽  
Latent Tuberculosis ◽  
Expression Level ◽  
Mrna Expression Level ◽  
Healthy Individuals ◽  
Relative Mrna Expression ◽  
Mrna Expression Levels

There have been few reports on the kinetics of hemodialyzed (HD) patients’ immune responses in latent tuberculosis infection (LTBI). Therefore, in the present study, messenger ribonucleic acid (mRNA) expression levels of nine immune markers were analyzed to discriminate between HD patients with LTBI and healthy individuals. Nine cytokines and chemokines were screened through relative mRNA expression levels in whole blood samples after stimulation with Mycobacterium tuberculosis (MTB)-specific antigens from HD patients with LTBI (HD/LTBI), HD patients without LTBI, and healthy individuals, and results were compared with the QuantiFERON-TB Gold In-Tube (QFT-GIT) test. We confirmed that the C-C motif chemokine 11 (CCL11) mRNA expression level of the HD/LTBI group was significantly higher than the other two groups. Especially, the CCL11 mRNA expression level of the >0.7 IU/mL group in the QFT-GIT test was significantly higher than the <0.2 IU/mL group in the QFT-GIT test and the 0.2–0.7 IU/mL group in the QFT-GIT test (p = 0.0043). The present study reveals that the relative mRNA expression of CCL11 was statistically different in LTBI based on the current cut-off value (i.e., ≥0.35 IU/mL) and in the >0.7 IU/mL group. These results suggest that CCL11 mRNA expression might be an alternative biomarker for LTBI diagnosis in HD patients.

Relationship Between the mRNA Expression Level of TGF-β Receptor Genes in Tissues and Ovulation Rate in Hu Sheep

2010 ◽  
Vol 9 (11) ◽  
pp. 1659-1666 ◽  
Author(s):  
Er-lin LI ◽  
Xin-hua XIE ◽  
Ye-fen XU ◽  
Zhuang XIE ◽  
Ling CHEN ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Ovulation Rate ◽  
Expression Level ◽  
Mrna Expression Level ◽  
Β Receptor ◽  
Hu Sheep
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