Astilbin Protects Against Carbon Tetrachloride-Induced Liver Fibrosis in Rats

Background: Hepatic fibrosis is an inflammatory liver disease, and there is no effective therapy at present. Astilbin is a bioactive ingredient found in many medicinal and food plants, with antioxidative, anti-inflammatory, and antitumor properties. Objectives: This study aimed to investigate the protective effect and related molecular mechanism of astilbin against carbon tetrachloride (CCl4)-induced liver fibrosis in rats. Methods: Liver fibrosis was induced by injection of CCl4 in male Sprague-Dawley rats, and those rats were then treated with astilbin at different concentrations. Pathological changes, collagen production, inflammatory cytokine, and oxidative stress were evaluated to evaluate the effects of astilbin on CCl4-induced hepatic fibrosis. Real-time PCR and western blot were performed to detect the mRNA and protein expression of indicated genes. Results: We discovered that CCl4 caused significant fibrosis damage in rat liver, and astilbin dose-dependently improved the liver functions and fibrosis degree. Astilbin treatment significantly decreased collagen production, inflammatory response, and oxidative stress in vivo. Mechanically, administration of astilbin obviously elevated the hepatic levels of Nrf2 and its downstream components, including NAD(P)H:quinone oxidoreductase 1 (Nqo1), heme oxygenase (HO-1), glutamate-cysteine ligase catalytic subunit, and glutamate cysteine ligase modifier. Conclusions: Taken together, these findings demonstrate that astilbin could protect against CCL4 induced-liver fibrosis in rats.

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Acupuncture Combined with Curcumin Attenuates Carbon Tetrachloride-Induced Hepatic Fibrosis in Rats

Acupuncture in Medicine ◽

10.1136/acupmed-2011-010116 ◽

2012 ◽

Vol 30 (2) ◽

pp. 132-138 ◽

Cited By ~ 6

Author(s):

Feng Zhang ◽

Jin Ma ◽

Yin Lu ◽

Guang-Xia Ni ◽

Chun-Yan Ni ◽

...

Keyword(s):

Carbon Tetrachloride ◽

Liver Fibrosis ◽

Hepatic Fibrosis ◽

Smooth Muscle Actin ◽

Sham Acupuncture ◽

Male Rats ◽

Sprague Dawley ◽

Combined Use ◽

Serum Aspartate Aminotransferase

Background Increasingly, studies demonstrate the effectiveness of acupuncture therapy against liver fibrosis. Curcumin is a natural product with antifibrotic effects, but has poor pharmacokinetic profiles. This study aimed to evaluate whether acupuncture combined with curcumin could more potently attenuate liver fibrosis in chemical intoxicated rats. Methods 60 Sprague–Dawley male rats were randomly divided into control, model, sham, acupuncture, curcumin and combination therapy groups. During the establishment of fibrosis using carbon tetrachloride (CCl4), acupuncture at LR3, LR14, BL18 and ST36 and/or curcumin treatment by mouth were performed simultaneously. After treatment, pathological indexes and histology for hepatic injury and fibrogenesis were detected. The expression of extracellular matrix (ECM) components was also determined. Results Acupuncture combined with curcumin potently protected the liver from CCl4-induced injury and fibrogenesis, as indicated by reduced levels of serum aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, hyaluronic acid, laminin and procollagen III. Combined use also led to significant liver histological improvements. Furthermore, combined use effectively inhibited ECM expression such as α-smooth muscle actin, fibronectin and α1(1) collagen. Conclusions Acupuncture treatment could significantly enhance the antifibrotic efficacy of curcumin on CCl4-induced hepatic fibrosis in rats in vivo, suggesting that a combination of acupuncture with curcumin may be exploited for the prevention of hepatic fibrosis.

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Inhibitory Effects of Raw-Extract Centella asiatica (RECA) on Acetylcholinesterase, Inflammations, and Oxidative Stress Activities via In Vitro and In Vivo

Molecules ◽

10.3390/molecules25040892 ◽

2020 ◽

Vol 25 (4) ◽

pp. 892 ◽

Cited By ~ 3

Author(s):

Zetty Zulikha Hafiz ◽

Muhammad ‘Afif Mohd Amin ◽

Richard Muhammad Johari James ◽

Lay Kek Teh ◽

Mohd Zaki Salleh ◽

...

Keyword(s):

Oxidative Stress ◽

Centella Asiatica ◽

Raw 264.7 Cells ◽

Dependent Manner ◽

Sprague Dawley ◽

Concentration Dependent Manner ◽

Sprague Dawley Rats ◽

And Oxidative Stress

Centella asiatica (C. asiatica) is one of the medicinal plants that has been reported to exert comprehensive neuroprotection in vitro and in vivo. In view of this, the present study was performed to investigate the effect of ethanolic extract of C. asiatica, designated as raw-extract of C. asiatica (RECA) in reducing the acetylcholinesterase (AChE), inflammations, and oxidative stress activities via both in vitro (SH-SY5Y and RAW 264.7 cells) and in vivo (Sprague Dawley rats). Quantitative high-performance liquid chromatography analysis reveals that RECA contains a significantly high proportion of glycosides than the aglycones with madecassoside as the highest component, followed by asiaticoside. Treatment of SH-SY5Y cells with RECA significantly reduced the AChE activity in a concentration-dependent manner with an IC50 value of 31.09 ± 10.07 µg/mL. Furthermore, the anti-inflammatory and antioxidant effects of RECA were evaluated by lipopolysaccharides (LPS)-stimulated RAW 264.7 cells. Our results elucidated that treatment with RECA significantly suppressed the level of pro-inflammatory cytokine/mediators and oxidative stress released in a concentration-dependent manner. Interestingly, these patterns of inhibition were consistent as observed in the LPS-induced neuroinflammation Sprague Dawley rats’ model. The highest concentration used in the two models presented the most significant results. Herein, our findings strongly suggest that RECA may offer therapeutic potential for the treatment of Alzheimer’s disease through inhibiting the AChE, inflammation, and oxidative stress activities.

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The Main Components Analysis of Laetiporus sulphureu Crude Extract and its Hepatoprotective Effect on Carbon Tetrachloride-Induced Hepatic Fibrosis in Rats

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.568-570.1934 ◽

2014 ◽

Vol 568-570 ◽

pp. 1934-1939

Author(s):

Wen Jun Sun ◽

Hai Bo He ◽

Jun Zhi Wang ◽

Ling Wu ◽

Fan Cheng ◽

...

Keyword(s):

Carbon Tetrachloride ◽

Liver Fibrosis ◽

Hepatic Fibrosis ◽

Liver Cell ◽

Cell Injury ◽

Cell Damage ◽

Hepatoprotective Effect ◽

Sprague Dawley ◽

Hepatoprotective Effects ◽

Main Components

To analysis the main component of Laetiporus sulphureus primary separation product (LSPSP) and observe the hepatoprotective effect of LSPSP. Silica column and HPLC was adopted to isolate main constituents from LSPSP. The main components were analyzed and identified by HPLC and HNMR. And then its hepatoprotective effect was evaluated, we used Sprague-Dawley rats as experimental rats, and made hepatic fibrosis models induced by carbon tetrachloride (CCl4).The hepatoprotective effects of SPSP on hepatic fibrosis of rats were evaluated by content of AST and ALT, levels of liver cell injury and degree of liver fibrosis. In the study, we found that LSPSP mainly contains eburicoic acid and trametenolic acid B. After treatment with LSPSP, the levels of serum AST and ALT significantly decreased, alleviated liver cell damage, reduced the collagen deposition in the liver tissue, and then inhibited liver fibrosis. Our studies indicated that LSPSP main components areeburicoic acid and trametenolic acid B, it exerted beneficially hepatoprotective effects on the CCl4 induced hepatic fibrosis.

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Canthin-6-one ameliorates TNBS-induced colitis in rats by modulating inflammation and oxidative stress. An in vivo and in silico approach

Biochemical Pharmacology ◽

10.1016/j.bcp.2021.114490 ◽

2021 ◽

Vol 186 ◽

pp. 114490

Author(s):

Karuppusamy Arunachalam ◽

Amilcar Sabino Damazo ◽

Antonio Macho ◽

Monica Steffi Matchado ◽

Eduarda Pavan ◽

...

Keyword(s):

Oxidative Stress ◽

In Silico ◽

And Oxidative Stress

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Total Antioxidant Capacity in HBV Carriers, a Promising Biomarker for Evaluating Hepatic Fibrosis: A Pilot Study

Antioxidants ◽

10.3390/antiox10010077 ◽

2021 ◽

Vol 10 (1) ◽

pp. 77

Author(s):

Jing-Hua Wang ◽

Sung-Bae Lee ◽

Dong-Soo Lee ◽

Chang-Gue Son

Keyword(s):

Oxidative Stress ◽

Chronic Hepatitis ◽

Liver Fibrosis ◽

Hepatitis B ◽

Antioxidant Capacity ◽

Chronic Hepatitis B ◽

Hepatic Fibrosis ◽

Total Antioxidant Capacity ◽

Hbv Dna ◽

Total Antioxidant

Oxidative stress plays a pivotal role in the progression of chronic hepatitis B; however, it is unclear whether the status of blood oxidative stress and antioxidant components differs depending on the degree of hepatic fibrosis. To explore the relationship between oxidative stress/antioxidant capacity and the extent of hepatic fibrosis, fifty-four subjects with liver fibrosis (5.5 ≤ liver stiffness measurement (LSM) score ≤ 16.0 kPa) by chronic hepatitis B virus (HBV) were analyzed. From the analysis of eight kinds of serum oxidative stress/antioxidant profiles and liver fibrosis degrees, the level of total antioxidant capacity (TAC) reflected a negative correlation with the severity of hepatic fibrosis (Pearson correlation, r = −0.35, p = 0.01). Moreover, TAC showed higher sensitivity (73.91%) than the aspartate transaminase (AST) to platelet ratio index (APRI, 56.52%) in the receiver operating characteristic (ROC) curves. Interestingly, the TAC level finely reflected the fibrosis degree in inactive carriers (HBV DNA < 2000 IU/mL), while the APRI did in active carriers (HBV DNA > 2000 IU/mL). In conclusion, TAC is a promising biomarker for evaluating the progression of liver fibrosis in patients with HBV, and this finding may indicate the involvement of TAC-composing factors in the pathogenesis of hepatic fibrosis in chronic HBV carriers.

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Impact of Polyphenolic-Food on Longevity: An Elixir of Life. An Overview

Antioxidants ◽

10.3390/antiox10040507 ◽

2021 ◽

Vol 10 (4) ◽

pp. 507

Author(s):

Rosaria Meccariello ◽

Stefania D’Angelo

Keyword(s):

Oxidative Stress ◽

Food Sources ◽

Preventive Action ◽

Nutritional Interventions ◽

Beneficial Effects ◽

Age Related ◽

Macromolecular Damage ◽

And Oxidative Stress

Aging and, particularly, the onset of age-related diseases are associated with tissue dysfunction and macromolecular damage, some of which can be attributed to accumulation of oxidative damage. Recently, growing interest has emerged on the beneficial effects of plant-based diets for the prevention of chronic diseases including obesity, diabetes, and cardiovascular disease. Several studies collectively suggests that the intake of polyphenols and their major food sources may exert beneficial effects on improving insulin resistance and related diabetes risk factors, such as inflammation and oxidative stress. They are the most abundant antioxidants in the diet, and their intake has been associated with a reduced aging in humans. Polyphenolic intake has been shown to be effective at ameliorating several age-related phenotypes, including oxidative stress, inflammation, impaired proteostasis, and cellular senescence, both in vitro and in vivo. In this paper, effects of these phytochemicals (either pure forms or polyphenolic-food) are reviewed and summarized according to affected cellular signaling pathways. Finally, the effectiveness of the anti-aging preventive action of nutritional interventions based on diets rich in polyphenolic food, such as the diets of the Blue zones, are discussed.

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Apigenin protects mice against 3,5-diethoxycarbonyl-1,4-dihydrocollidine-induced cholestasis

Food & Function ◽

10.1039/d0fo02910f ◽

2021 ◽

Vol 12 (5) ◽

pp. 2323-2334

Author(s):

Shihong Zheng ◽

Peichang Cao ◽

Zequn Yin ◽

Xuerui Wang ◽

Yuanli Chen ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Metabolism ◽

Liver Fibrosis ◽

Liver Damage ◽

Liver Diseases ◽

Potential Drug ◽

Abnormal Lipid Metabolism ◽

And Oxidative Stress

Apigenin prevented the DDC-induced abnormal lipid metabolism, liver damage and liver fibrosis by reducing inflammation and oxidative stress. Apigenin might be a potential drug for the treatment of cholestatic liver diseases.

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Pirfenidone mediates cigarette smoke extract induced inflammation and oxidative stress in vitro and in vivo

International Immunopharmacology ◽

10.1016/j.intimp.2021.107593 ◽

2021 ◽

Vol 96 ◽

pp. 107593

Author(s):

Yiming Ma ◽

Lijuan Luo ◽

Xiangming Liu ◽

Herui Li ◽

Zihang Zeng ◽

...

Keyword(s):

Oxidative Stress ◽

Cigarette Smoke ◽

Cigarette Smoke Extract ◽

And Oxidative Stress

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Galangin Resolves Cardiometabolic Disorders through Modulation of AdipoR1, COX-2, and NF-κB Expression in Rats Fed a High-Fat Diet

Antioxidants ◽

10.3390/antiox10050769 ◽

2021 ◽

Vol 10 (5) ◽

pp. 769

Author(s):

Patoomporn Prasatthong ◽

Sariya Meephat ◽

Siwayu Rattanakanokchai ◽

Juthamas Khamseekaew ◽

Sarawoot Bunbupha ◽

...

Keyword(s):

Oxidative Stress ◽

Cardiac Tissue ◽

Sprague Dawley ◽

High Fat ◽

Impaired Liver Function ◽

Impaired Liver ◽

Cardiometabolic Disorders ◽

Cox 2 ◽

Factor Α ◽

And Oxidative Stress

Galangin is a natural flavonoid. In this study, we evaluated whether galangin could alleviate signs of metabolic syndrome (MS) and cardiac abnormalities in rats receiving a high-fat (HF) diet. Male Sprague–Dawley rats were given an HF diet plus 15% fructose for four months, and they were fed with galangin (25 or 50 mg/kg), metformin (100 mg/kg), or a vehicle for the last four weeks. The MS rats exhibited signs of MS, hypertrophy of adipocytes, impaired liver function, and cardiac dysfunction and remodeling. These abnormalities were alleviated by galangin (p < 0.05). Interleukin-6 and tumor necrosis factor-α concentrations and expression were high in the plasma and cardiac tissue in the MS rats, and these markers were suppressed by galangin (p < 0.05). These treatments also alleviated the low levels of adiponectin and oxidative stress induced by an HF diet in rats. The downregulation of adiponectin receptor 1 (AdipoR1) and cyclooxygenase-2 (COX-2) and the upregulation of nuclear factor kappa B (NF-κB) expression were recovered in the galangin-treated groups. Metformin produced similar effects to galangin. In conclusion, galangin reduced cardiometabolic disorders in MS rats. These effects might be linked to the suppression of inflammation and oxidative stress and the restoration of AdipoR1, COX-2, and NF-κB expression.

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