scholarly journals Monitoring of Erosive Tooth Wear with Intraoral Scanners In vitro

2021 ◽  
pp. 1-10
Author(s):  
Corinna Witecy ◽  
Carolina Ganss ◽  
Bernd Wöstmann ◽  
Moritz B. Schlenz ◽  
Maximiliane A. Schlenz
Keyword(s):  
In Vitro Study ◽  
Tooth Wear ◽  
Systematic Difference ◽  
Tissue Loss ◽  
Load Bearing ◽  
Erosive Tooth Wear ◽  
Promising Tool ◽  
External Software ◽  
Optical Profilometer

Intraoral scanners (IOS) have been used to quantify tooth wear, but so far they have not been systematically validated for monitoring of tissue loss. The aim of this in vitro study was to investigate whether progression of tissue loss can be detected with an IOS and whether IOS values agree with those obtained with noncontacting profilometry (PRO) serving as a standard method. Model jaws were mounted in a phantom head positioned in a dental chair. Flattened areas were prepared on the non-load-bearing cusps of the first molars (model teeth; n = 16) in order to fix flat enamel samples with an experimental area and a reference area. After baseline PRO and IOS, the experimental enamel area was stepwise etched with 35% H3PO4 gel (4 × 30 s and 4 × 15 s). After each etching, PRO and IOS was performed and the vertical tissue loss between the reference and experimental areas was measured, each at the same 3 measurement points. Furthermore, cupped cusps were simulated by stepwise preparation of the load-bearing cusps of the model teeth with a spherical diamond bur, and the maximum vertical depth after each preparation step was measured only by IOS. Trios3 (3Shape, Denmark), Carestream CS3600 (Carestream, USA) and an optical profilometer (MicroProf, Fries, Germany) were used to measure the flat areas of the enamel samples, whereas only IOS were used to measure curved surfaces on the load-bearing cupped cusps of the model teeth. The IOS data were analyzed with an external software (GOM Inspect, Germany) and with the respective internal IOS software. PRO revealed a mean (±SD) tissue loss of 17.1 ± 4.7 µm after 30-s etching steps and 10.1 ± 5.1 µm after the 15-s etching steps. IOS and software types were able to detect the progression of tissue loss after each etching step (p ≤ 0.001 each); Bland-Altmann plots revealed good agreement with PRO regardless of the order of tissue loss, and no systematic difference was found. Increasing cupped lesion depths were detected by all IOS, with no significant differences between IOS and analysis methods. IOS were able to detect small amounts of tissue loss under simulated clinical conditions and seem to be a promising tool for monitoring even initial erosive tooth wear.

scholarly journals Potential of different fluoride gels to prevent erosive tooth wear caused by gastroesophageal reflux

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Philipp Körner ◽  
Luca Georgis ◽  
Daniel B. Wiedemeier ◽  
Thomas Attin ◽  
Florian J. Wegehaupt
Keyword(s):  
Gastroesophageal Reflux ◽  
Artificial Saliva ◽  
In Vitro Study ◽  
Tooth Wear ◽  
Control Group ◽  
Erosive Tooth Wear ◽  
Custom Made ◽  
Post Hoc ◽  
Bovine Enamel

Abstract Background This in-vitro-study aimed to evaluate the potential of different fluoride gels to prevent gastroesophageal reflux induced erosive tooth wear. Methods Surface baseline profiles of a total of 50 bovine enamel specimens [randomly assigned to five groups (G1–5)] were recorded. All specimens were positioned in a custom made artificial oral cavity and perfused with artificial saliva (0.5 ml/min). Reflux was simulated 11 times a day during 12 h by adding HCl (pH 3.0) for 30 s (flow rate 2 ml/min). During the remaining 12 h (overnight), specimens were stored in artificial saliva and brushed twice a day (morning and evening) with a toothbrush and toothpaste slurry (15 brushing strokes). While specimens in the control group (G1) did not receive any further treatment, specimens in G2–5 were coated with different fluoride gels [Elmex Gelée (G2); Paro Amin Fluor Gelée (G3); Paro Fluor Gelée Natriumfluorid (G4); Sensodyne ProSchmelz Fluorid Gelée (G5)] in the evening for 30 s. After 20 days, surface profiles were recorded again and enamel loss was determined by comparing them with the baseline profiles. The results were statistically analysed using one-way analysis of variance (ANOVA) followed by Tukey`s HSD post-hoc test. Results The overall highest mean wear of enamel (9.88 ± 1.73 µm) was observed in the control group (G1), where no fluoride gel was applied. It was significantly higher (p < 0.001) compared to all other groups. G2 (5.03 ± 1.43 µm), G3 (5.47 ± 0.63 µm, p = 0.918) and G4 (5.14 ± 0.82 µm, p > 0.999) showed the overall best protection from hydrochloric acid induced erosion. Enamel wear in G5 (6.64 ± 0.86 µm) was significantly higher compared to G2 (p = 0.028) and G4 (p = 0.047). Conclusions After 20 days of daily application, all investigated fluoride gels are able to significantly reduce gastroesophageal reflux induced loss of enamel.

scholarly journals Impact of mucin on the anti-erosive/anti-abrasive efficacy of chitosan and/or F/Sn in enamel in vitro

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Benedikt Luka ◽  
Vivien Arbter ◽  
Kathrin Sander ◽  
Andrea Duerrschnabel ◽  
Nadine Schlueter
Keyword(s):  
In Vitro Study ◽  
Tooth Wear ◽  
Tissue Loss ◽  
Hard Tissue ◽  
Porcine Gastric Mucin ◽  
Human Enamel ◽  
Enamel Erosion ◽  
Saliva Flow ◽  
Positively Charged

AbstractThe application of stannous ions in combination with fluoride (F/Sn) is one of the central strategies in reducing erosive tooth wear. F/Sn efficacy can be enhanced by adding chitosan, a positively charged biopolymer. For patients with low saliva flow, this efficacy, however, is not sufficient, making further improvement desirable. This could be achieved by combining chitosan with other molecules like mucin, which together might form multilayers. This in-vitro study aimed to investigate the effect of chitosan, mucin, F/Sn and combinations thereof on enamel erosion and erosion-abrasion. Human enamel samples (n = 448, 28 groups) were cyclically eroded or eroded-abraded (10 days; 6 × 2 min erosion and 2 × 15 s/200 g abrasion per day). Samples were treated 2 × 2 min/day with solutions containing either, chitosan (50 or 500 mPas), porcine gastric mucin, F/Sn or combinations thereof after abrasive challenge. Tissue loss was measured profilometrically, interaction between hard tissue and active agents was assessed with energy dispersive spectroscopy and scanning electron microscopy. Chitosan and F/Sn showed the expected effect in reducing tissue loss under erosive and under erosive-abrasive conditions. Neither mucin alone nor the combinations with mucin showed any additional beneficial effect.

scholarly journals The effect of design parameters of interspinous implants on kinematics and load bearing: an in vitro study

2014 ◽  
Vol 23 (4) ◽  
pp. 762-771 ◽  
Author(s):  
Christoph Schilling ◽  
M. Pfeiffer ◽  
T. M. Grupp ◽  
W. Blömer ◽  
A. Rohlmann
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Design Parameters ◽  
Load Bearing ◽  
Interspinous Implants

scholarly journals Tooth wear prevention: A quantitative and qualitative in vitro study

2003 ◽  
Vol 48 (1) ◽  
pp. 15-19 ◽  
Author(s):  
JA Kaidonis ◽  
J. Gratiaen ◽  
N. Bhatia ◽  
LC Richards ◽  
GC Townsend
Keyword(s):  
In Vitro Study ◽  
Tooth Wear ◽  
Vitro Study

scholarly journals The effect of design parameters of dynamic pedicle screw systems on kinematics and load bearing: an in vitro study

2010 ◽  
Vol 20 (2) ◽  
pp. 297-307 ◽  
Author(s):  
C. Schilling ◽  
S. Krüger ◽  
T. M. Grupp ◽  
G. N. Duda ◽  
W. Blömer ◽  
...  
Keyword(s):  
Pedicle Screw ◽  
In Vitro Study ◽  
Vitro Study ◽  
Design Parameters ◽  
Load Bearing

scholarly journals Reconstruction of Pulpotomized Primary Molar and Retention of Stainless-steel Crowns: An In-vitro Study

2020 ◽  
Vol 14 (1) ◽  
pp. 511-514
Author(s):  
Mehrshad Zareiyan ◽  
Fatemeh Molaasadolah ◽  
Roza Haghgoo ◽  
Rahil Ahmadi ◽  
Mehrdad Kahvand
Keyword(s):  
Stainless Steel ◽  
Testing Machine ◽  
Glass Ionomer Cement ◽  
In Vitro Study ◽  
Pediatric Dentistry ◽  
Tissue Loss ◽  
Dental Tissue ◽  
Primary Molar ◽  
Tissue Reconstruction

Background: Stainless steel crowns are proposed to restore severely decayed teeth in pediatric dentistry. Yet, their retention is still debatable. Objective: This study aimed to investigate whether dental tissue reconstruction before placement of stainless steel crown on pulpotomized first primary molar affects the retention of stainless steel crowns. Methods: This experimental in-vitro study was performed on 30 extracted first primary molars with carious occlusal and distal surfaces. Dental caries and the pulp chamber roof were respectively removed. The stainless steel crowns were placed after tooth preparation. The samples were randomly divided into two groups (n=15) i.e, ZoE core buildup, and ZoE liner. Stainless steel crowns were cemented with self-curing glass ionomer cement. The crown retention was tested with the Instron testing machine. The two groups were compared by using SPSS software through the t-test (α=0.05). Results: The mean crown retention in the liner group (291.45±43.196 N) was significantly higher than that of the core buildup group (202.00±63.515 N) (P=0.001). Conclusion: Based on the results of this study for restoring the teeth with extensive tissue loss by SSC, ZoE should be used as lining without a core on teeth.

scholarly journals IMPACT OF LIGHT AND SUGAR-FREE BEVERAGES ON DENTIN EROSION - AN IN VITRO STUDY

2014 ◽  
Vol 2 (2) ◽  
pp. 159
Author(s):  
Ana Luiza Ubinha Moino ◽  
Catarina Ribeiro Barros Alencar ◽  
Maisa Camilo Jordão ◽  
Marília Afonso Rabelo Buzalaf ◽  
Ana Carolina Magalhães ◽  
...  
Keyword(s):  
Fruit Juice ◽  
Artificial Saliva ◽  
Erosive Wear ◽  
In Vitro Study ◽  
Tooth Wear ◽  
Passion Fruit ◽  
Root Dentin ◽  
Bonferroni Test ◽  
Dentin Erosion

AIM: To compare the erosive effect of different light and sugar free beverages in relation to their regular version, on dentin. MATERIALS AND METHODS: Bovine root dentin samples were randomized into 9 different types of drinks (each n=12): Coca-cola®, Light Coca-cola®, Coca-cola® Zero, Guaraná Antartica®, Guaraná Antartica® Zero, Sprite®, Sprite® Zero, Del Valle® Passion fruit juice and Del Valle® Light Passion fruit juice. During each one of the 10 experimental days, the samples were subjected to 3 erosive demineralization (by immersion of the sample in the studied drink) and remineralization (by immersion of the sample in artificial saliva) cycles. Profilometry was evaluated at the end of the 1st, 5th and 10th days. For each type of drink, the data were tested using 2 way ANOVA and Bonferroni test (p<0.05). RESULTS: Erosive cycling with regular cola resulted in a higher wear on the first day, on the 5th day the regular drink was similar to zero and greater than light version, on the 10th day there was no difference between the versions of cola drink. For the Sprite drink there was difference between the versions just in the 10th day, in which the higher wear was promoted by the zero version. For guaraná and juice beverages there was no differences between versions in relation to the resulted erosive wear. CONCLUSION: Erosive tooth wear promoted by light and sugar-free or regular versions of soft drinks and juices showed distinct behaviours over the cycling time.

scholarly journals Ratiometric Polymer Probe for Detection of Peroxynitrite and the Application for Live-Cell Imaging

Molecules ◽  
2019 ◽  
Vol 24 (19) ◽  
pp. 3465
Author(s):  
Hio Kuan Lao ◽  
Jingyun Tan ◽  
Chunfei Wang ◽  
Xuanjun Zhang
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
In Vitro Study ◽  
Water Soluble ◽  
Fluorescence Intensity Ratio ◽  
Oxidation Stress ◽  
Promising Tool ◽  
Good Biocompatibility

Peroxynitrite (ONOO−) is one of the sources of oxidation stress involved in many biological signaling pathways. The role of ONOO− being a double-edged sword in biological systems drives the development of effective detection tools. In this work, a boronate-based polymeric fluorescent probe PB-PVA was synthesized and the probe performance was evaluated. The probe exhibits ratiometric sensing of ONOO− in a range of 0–6 µM. There is good linear relationship between the probe fluorescence intensity ratio and ONOO− concentration. The probe also displays moderate selectivity towards ONOO− over other ROS. Moreover, it is water-soluble and possesses good biocompatibility which aids the imaging of ONOO− in living cells. These properties could make the probe a promising tool in in vitro study related to ONOO−.

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