scholarly journals SLEEP DEPRIVATION INTERFERES WITH JAK/STAT SIGNALING PATHWAY AND MYOGENESIS IN MASSETER MUSCLE OF RATS

2021 ◽  
Author(s):  
Marina Gomes Galvani ◽  
Hanna Karen Moreira Antunes ◽  
Marcos Monico-Neto ◽  
Veronica Quispe Yujra ◽  
Carla Maximo Prado ◽  
...  
Keyword(s):  
Sleep Deprivation ◽  
Signaling Pathway ◽  
Masseter Muscle ◽  
Paradoxical Sleep ◽  
Paradoxical Sleep Deprivation ◽  
Cellular Density ◽  
Stat Signaling ◽  
Male Wistar Rats ◽  
Profile Area

jectives. This study evaluated the JAK/STAT signaling pathway and myogenesis on masseter muscle after sleep deprivation as well as to investigate the role of stress in this scenario. Subjetcts and Methods. A total of 18 male Wistar rats were distributed into the following groups: Control (CTRL; n=6): animals were not submitted to any procedures; Sleep Deprivation and Vehicle (PSD+V; n=6): animals were subjected to Paradoxical Sleep Deprivation for 96h and (PSD+MET; n=6): animals were subjected to Paradoxical Sleep Deprivation for 96h with administration of metyrapone. Paradoxal Sleep deprivation was performed by the Modified Multiple Platforms Method. Histopatological analysis, histomorphmetry and immunohistochemistry were performed. Results and Conclusion. The results showed the presence of inflammatory infiltrate in the PSD+V and PSD+MET groups and atrophy. Histomorphometry showed that Cellular Profile Area decreased while Cellular Density increased in both experimental groups. Expression of p-STAT 3, MyoD and MyoG increased in PSD+V group, while the PSD+MET group increased expression of IL-6 and p-STAT 3. Our results are consistent with notion that sleep deprivation induced inflammatory response and atrophy in masseter muscle of rats.

Role of epigenetic regulatory enzymes in animal models of mania induced by amphetamine and paradoxical sleep deprivation

2020 ◽  
Author(s):  
Roger B Varela ◽  
Wilson R Resende ◽  
Gustavo C Dal‐Pont ◽  
Fernanda F Gava ◽  
Gabriella B Nadas ◽  
...  
Keyword(s):  
Animal Models ◽  
Sleep Deprivation ◽  
Paradoxical Sleep ◽  
Paradoxical Sleep Deprivation ◽  
Regulatory Enzymes

scholarly journals Cytokine and microRNA levels during different periods of paradoxical sleep deprivation and sleep recovery in rats

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5567 ◽  
Author(s):  
Malinalli Brianza-Padilla ◽  
Fausto Sánchez-Muñoz ◽  
Gonzalo Vázquez-Palacios ◽  
Fengyang Huang ◽  
Julio César Almanza-Pérez ◽  
...  
Keyword(s):  
Sleep Deprivation ◽  
Wistar Rats ◽  
Inflammatory Responses ◽  
Paradoxical Sleep ◽  
Serum Levels ◽  
Paradoxical Sleep Deprivation ◽  
Short Term ◽  
Threefold Increase ◽  
Male Wistar Rats

Background Sleep has a fundamental role in the regulation of homeostasis. The aim of this study was to assess the effect of different periods of paradoxical sleep deprivation (PSD) and recovery on serum levels of cytokines and miRNAs related to inflammatory responses. Methods Male Wistar rats were submitted to a PSD of 24, 96, or 192 h, or of 192 h followed by 20 days of recovery (192 h PSD+R). The concentrations of corticosterone, cytokines (IL-6, TNF, IL-10, Adiponectin) and miRNAs (miR-146a, miR-155, miR-223, miR-16, miR-126, miR-21) in serum were evaluated. Results At PSD 24 h a significant increase of IL-6 and decrease of IL-10 were observed. At PSD 96h adiponectin increased. At 192 h of PSD IL-6 increased significantly again, accompanied by a threefold increase of IL-10 and an increase of serum corticosterone. After 20 days of recovery (192 h PSD+R) corticosterone, IL-6 and TNF levels increased significantly, while IL-10 decreased also significantly. Regarding the miRNAs at 24 h of PSD serum miR-146a, miR-155, miR-223, and miR-16 levels all increased. At 96 h of PSD miR-223 decreased. At 192 h of PSD decreases in miR-16 and miR-126 were observed. After recovery serum miR-21 increased and miR-16 decreased. Conclusion PSD induces a dynamic response likely reflecting the induced cellular stress and manifested as variating hormonal and inflammatory responses. Sleep deprivation disturbed corticosterone, cytokine and miRNA levels in serum related to the duration of sleep deprivation, as short-term PSD produced effects similar to those of an acute inflammatory response and long-term PSD induced long-lasting disturbances of biological mediators.

Paradoxical sleep deprivation induces oxidative stress in the submandibular glands of Wistar rats

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Taye J. Lasisi ◽  
Shehu-Tijani T. Shittu ◽  
Jude I. Abeje ◽  
Kehinde J. Ogunremi ◽  
Seyyid A. Shittu
Keyword(s):  
Oxidative Stress ◽  
Sleep Deprivation ◽  
Wistar Rats ◽  
Paradoxical Sleep ◽  
Salivary Secretion ◽  
Control Group ◽  
Paradoxical Sleep Deprivation ◽  
Sod Activity ◽  
Submandibular Glands ◽  
Male Wistar Rats

Abstract Objectives Paradoxical sleep deprivation has been associated with impaired salivary secretion in rats. However, the mechanism that underlies this is not known. Therefore, this study assessed salivary and serum oxidative stress levels following paradoxical sleep deprivation in rats. Methods Twenty-one male Wistar rats randomly divided into three groups of seven rats each as; Control (C); partial sleep-deprived (PSD); and total sleep-deprived (TSD) were used. Malondialdehyde (MDA) concentration, Superoxide dismutase (SOD), and catalase activities were evaluated in saliva, serum, and submandibular glands after seven days of sleep deprivation. Data were expressed as mean ± standard error of the mean and analyzed using one-way ANOVA, Tukey HSD post hoc, and Pearson’s correlation tests. Results Serum MDA levels were significantly higher in both the TSD and PSD groups compared to the control group whereas only the TSD group showed higher submandibular MDA levels compared to the PSD group and the control group. Submandibular SOD activity was significantly lower in both the TSD and PSD groups compared to the control group. Serum catalase activity was significantly lower in the TSD group only compared to the control group. Conclusions These results have demonstrated for the first time that paradoxical sleep deprivation was associated with changes in the oxidant/antioxidant defense system in the submandibular salivary glands of male Wistar rats which may contribute to impairment in salivary secretion.

The Pronociceptive Effect of Paradoxical Sleep Deprivation in Rats: Evidence for a Role of Descending Pain Modulation Mechanisms

2015 ◽  
Vol 53 (3) ◽  
pp. 1706-1717 ◽  
Author(s):  
Dabna H. Tomim ◽  
Felipe M. Pontarolla ◽  
Jessica F. Bertolini ◽  
Mauricio Arase ◽  
Glaucia Tobaldini ◽  
...  
Keyword(s):  
Sleep Deprivation ◽  
Paradoxical Sleep ◽  
Pain Modulation ◽  
Paradoxical Sleep Deprivation ◽  
Descending Pain Modulation

Effects of different periods of paradoxical sleep deprivation and sleep recovery on lipid and glucose metabolism and appetite hormones in rats

2016 ◽  
Vol 41 (3) ◽  
pp. 235-243 ◽  
Author(s):  
Malinalli Brianza-Padilla ◽  
Herlinda Bonilla-Jaime ◽  
Julio César Almanza-Pérez ◽  
Ana Laura López-López ◽  
Fausto Sánchez-Muñoz ◽  
...  
Keyword(s):  
Sleep Deprivation ◽  
Paradoxical Sleep ◽  
Low Density Lipoprotein ◽  
Recovery Period ◽  
Density Lipoprotein ◽  
Tolerance Test ◽  
Oral Glucose ◽  
Low Density ◽  
Paradoxical Sleep Deprivation ◽  
Male Wistar Rats

Sleep has a fundamental role in the regulation of energy balance, and it is an essential and natural process whose precise impacts on health and disease have not yet been fully elucidated. The aim of this study was to assess the consequences of different periods of paradoxical sleep deprivation (PSD) and recovery from PSD on lipid profile, oral glucose tolerance test (OGTT) results, and changes in insulin, corticosterone, ghrelin, and leptin concentrations. Three-month-old male Wistar rats weighing 250–350 g were submitted to 24, 96, or 192 h of PSD or 192 h of PSD with 480 h of recovery. The PSD was induced by the multiple platforms method. Subsequently, the animals were submitted to an OGTT. One day later, the animals were killed and the levels of triglycerides, total cholesterol, lipoproteins (low-density lipoprotein, very-low-density lipoprotein, and high-density lipoprotein), insulin, ghrelin, leptin, and corticosterone in plasma were quantified. There was a progressive decrease in body weight with increasing duration of PSD. The PSD induced basal hypoglycemia over all time periods evaluated. Evaluation of areas under the curve revealed progressive hypoglycemia only after 96 and 192 h of PSD. There was an increase in corticosterone levels after 192 h of PSD. We conclude that PSD induces alterations in metabolism that are reversed after a recovery period of 20 days.

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