Morphological and molecular characterisation of Aphelenchoides besseyi and A. fujianensis (Nematoda: Aphelenchoididae) from rice and forage grass seeds in Brazil

Nematology ◽  
2016 ◽  
Vol 18 (3) ◽  
pp. 337-356 ◽  
Author(s):  
Dalila Sêni de Jesus ◽  
Claudio Marcelo Gonçalves Oliveira ◽  
David Roberts ◽  
Vivian Blok ◽  
Roy Neilson ◽  
...  

Morphologically similarAphelenchoidesspp. populations extracted from rice and forage grass seeds from different geographical regions in Brazil were morphologically and molecularly characterised. Overall, the populations studied separated into two groups based on morphological and phylogenetic analyses, referred to herein as ‘Group-rice’ and ‘Group-forage’. Bayesian phylogenetic analyses of SSU, LSU and mtCOI regions strongly supported the presence of two dichotomous groups with Group-rice and Group-forage populations genetically similar toA. besseyiandA. fujianensis, respectively. This study reports the presence of a morphologically similar species toA. besseyiassociated with seeds of grasses, but genetically distinct based on three genomic regions, which our results strongly suggest to beA. fujianensis, this being a new geographical record for Brazil. Additional information regarding spicule morphology of maleA. besseyiis also reported.

Parasitology ◽  
2019 ◽  
Vol 146 (13) ◽  
pp. 1673-1682 ◽  
Author(s):  
Tanapan Sukee ◽  
Ian Beveridge ◽  
Neil B. Chilton ◽  
Abdul Jabbar

AbstractThe genetic variation and taxonomic status of the four morphologically-defined species of Macropostrongyloides in Australian macropodid and vombatid marsupials were examined using sequence data of the ITS+ region (=first and second internal transcribed spacers, and the 5.8S rRNA gene) of the nuclear ribosomal DNA. The results of the phylogenetic analyses revealed that Ma. baylisi was a species complex consisting of four genetically distinct groups, some of which are host-specific. In addition, Ma. lasiorhini in the common wombat (Vombatus ursinus) did not form a monophyletic clade with Ma. lasiorhini from the southern hairy-nosed wombat (Lasiorhinus latifrons), suggesting the possibility of cryptic (genetically distinct but morphologically similar) species. There was also some genetic divergence between Ma. dissimilis in swamp wallabies (Wallabia bicolor) from different geographical regions. In contrast, there was no genetic divergence among specimens of Ma. yamagutii across its broad geographical range or between host species (i.e. Macropus fuliginosus and M. giganteus). Macropostrongyloides dissimilis represented the sister taxon to Ma. baylisi, Ma. yamagutii and Ma. lasiorhini. Further morphological and molecular studies are required to assess the species complex of Ma. baylisi.


2021 ◽  
Vol 7 (11) ◽  
pp. 945
Author(s):  
Jing Yang ◽  
Ling-Ling Liu ◽  
E. B. Gareth Jones ◽  
Wen-Li Li ◽  
Kevin D. Hyde ◽  
...  

Distoseptispora is one of the sporidesmium-like taxa with great variation in asexual morphology and delineation of species. Phylogenetic analyses of four gene regions LSU, ITS, TEF1α, and RPB2 revealed the placement of several sporidesmium-like species in Distoseptispora (Distoseptisporaceae, Distoseptisporales, Sordariomycetes), collected on submerged decaying twigs from streams in China and Thailand. Based on morphological examination and molecular DNA data, six new species, Distoseptispora amniculi, D. atroviridis, D. effusa, D. fusiformis, D. hyalina, and D. verrucosa, are proposed. Among them, D. hyalina is the first sexual morph confirmed in the genus. A new geographical record is reported for D. lignicola in China. Conidial length proved to be of less taxonomic significance for some Distoseptispora species, whereas the type of conidial septa is informative at species level.


Phytotaxa ◽  
2021 ◽  
Vol 518 (2) ◽  
pp. 143-166
Author(s):  
LIN SHI ◽  
HAO YANG ◽  
KEVIN D. HYDE ◽  
NALIN N. WIJAYAWARDENE ◽  
GEN-NUO WANG ◽  
...  

Eight freshwater asexual strains collected from China and Thailand were subjected to morpho-molecular analyses. BLAST search results showed that the new strains belonged to Pleurotheciales, Sordariomycetes. Phylogenetic analyses based on a combined LSU, SSU, ITS and RPB2 sequence dataset are used to clarify the placement of the new strains within Pleurotheciales. Pleurothecium guttulatum and Pleurotheciella sympodia are introduced as two new species from freshwater habitats. New geographical record of Pleurotheciella nilotica from Asia (Thailand and China) is reported. New habitat and geographical records of Phaeoisaria annesophieae (from freshwater habitats in Asia) are reported. Descriptions and illustrations of the new species and records, as well as known species Rhexoacrodictys erecta and Phaeoisaria annesophieae, are provided. A key to Pleurotheciella species is provided.


Author(s):  
P. Strafella ◽  
V. Salvalaggio ◽  
C. Cuicchi ◽  
E. Punzo ◽  
A. Santelli ◽  
...  

AbstractThree cumacean species, Eudorella nana Sars, 1879, Leucon affinis Fage, 1951, Leucon siphonatus Calman, 1905, were recorded for the first time and one rare amphipod presence, Stenothoe bosphorana Sowinsky, 1898, was confirmed in the Adriatic basin.


Phytotaxa ◽  
2018 ◽  
Vol 346 (2) ◽  
pp. 157
Author(s):  
C. BIJEESH ◽  
A. MANOJ KUMAR ◽  
K.B. VRINDA ◽  
C.K. PRADEEP

Two unusual species of Craterellus have been collected numerous times from the evergreen tropical forests in Kerala State, India. The species Craterellus albostrigosus and Craterellus inusitatus are described as new based on morphological and phylogenetic analyses of nrLSU-rDNA gene region. Complete morphological descriptions, photographs and comparisons with similar species are provided as well as a key to the known species of Craterellus from India.


Zootaxa ◽  
2021 ◽  
Vol 4995 (2) ◽  
pp. 334-344
Author(s):  
QIAN ZHOU ◽  
FAHUI TANG ◽  
YUANJUN ZHAO

During a survey of parasitic ciliates in Chongqing, China, Trichodina matsu Basson & Van As, 1994 was isolated from gills of Tachysurus fulvidraco. Furthermore, the 18S rRNA gene and ITS-5.8S rRNA region of T. matsu were sequenced for the first time and applied for the species identification and comparison with similar species in the present study. Based on the morphological and molecular comparisons, the results indicate that T. matsu is an ectoparasite specific for the Siluriformes catfish. Based on the analyses of genetic distance, multiple sequence alignments, and phylogenetic analyses, no obvious differentiation within populations of T. matsu was found. In addition, the ‘Trichodina hyperparasitis’ (KX904933) in GenBank is a misidentification and appears to be conspecific with T. matsu according to the comparison of morphological and molecular data.  


2021 ◽  
Vol 11 ◽  
Author(s):  
Fenggang Yu ◽  
Nicholas L. Syn ◽  
Yanan Lu ◽  
Qing Yun Chong ◽  
Junyun Lai ◽  
...  

Epstein-Barr virus (EBV)—the prototypical human tumor virus—is responsible for 1–2% of the global cancer burden, but divergent strains seem to exist in different geographical regions with distinct predilections for causing lymphoid or epithelial malignancies. Here we report the establishment and characterization of Yu103, an Asia Pacific EBV strain with a highly remarkable provenance of being derived from nasopharyngeal carcinoma biopsy but subsequently propagated in human B-lymphoma cells and xenograft models. Unlike previously characterized EBV strains which are either predominantly B-lymphotropic or epitheliotropic, Yu103 evinces an uncanny capacity to infect and transform both B-lymphocytes and nasopharyngeal epithelial cells. Genomic and phylogenetic analyses indicated that Yu103 EBV lies midway along the spectrum of EBV strains known to drive lymphomagenesis or carcinogenesis, and harbors molecular features which likely account for its unusual properties. To our knowledge, Yu103 EBV is currently the only EBV isolate shown to drive human nasopharyngeal carcinoma and B-lymphoma, and should therefore provide a powerful novel platform for research on EBV-driven hematological and epithelial malignancies.


2021 ◽  
Vol 71 ◽  
pp. 55-74
Author(s):  
Juan José Torres-Ramírez ◽  
Teddy Angarita-Sierra ◽  
Mario Vargas-Ramírez

In northern South America, amphisbaenians are rarely seen among the herpetofauna.Thus, general knowledge about them is very poor. During a herpetological survey in 2012 at Casanare, Colombia, we found two specimens of an unusual Amphisbaena. A third specimen sharing the same morphotype labeled Amphisbaena sp. from Vichada department was found deposided in an Colombian reptile collection. Based on morphological analyses together with phylogenetic analyses of 1029 base pairs of the mitochondrial DNA (mtDNA), we describe a new species of Amphisbaena that inhabits in the Orinoquian region of Colombia. The new species is part of a phylogenetic clade together with A. mertensii and A. cunhai (central-southern Brazil), exhibiting a great genetic distance (26.1–28.9%) between the newly identified lineage versus those taxa, and versus the sympatric taxa A. alba and A. fuliginosa. Morphologically, this new Amphisbaena can be distinguished from their congeners by characters combination of number of preocloacal pores, absence of malar scale, postgenial scales and body and caudal annuli counts. Amphisbaena gracilis is on morphology grounds the most similar species. However, the new species can be distinguished from it by having higher body annuli counts, angulus ories aliegned with the edges of the ocular scales and center of frontal scales, less number of large middorsal segments of the first and second body annulus, and rostral scale visible from above. The description of this new Amphisbaena species points out the urgent need to increase the knowledge of worm lizards in Colombia


Phytotaxa ◽  
2021 ◽  
Vol 500 (1) ◽  
pp. 1-10
Author(s):  
MENG-LE XIE ◽  
TIE-ZHENG WEI ◽  
BÁLINT DIMA ◽  
YONG-PING FU ◽  
RUI-QING JI ◽  
...  

This study presents one telamonioid species new to science based on morphological characteristics and molecular phylogenetic analyses. Cortinarius khinganensis was collected from the Greater Khingan Mountains, Northeast China and it is characterized by hygrophanous, vivid brownish red and striate pileus, white universal veil, and subglobose spores. According to phylogenetic analyses results, C. khinganensis belongs to the section Illumini, which is a lineage distantly related from subgenus Telamonia sensu stricto. Detailed descriptions of the new species and the comparisons with morphologically similar species are provided. The phylogenetic relationships within the section Illumini are also discussed.


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