The first assessment of genetic diversity among Turkish leek accessions using molecular and morphological markers

2021 ◽  
pp. 1-10
Author(s):  
Fatih Hanci
Keyword(s):  
Genetic Diversity ◽  
Molecular Genetic ◽  
Morphological Characters ◽  
Issr Markers ◽  
Morphological Data ◽  
Local Varieties ◽  
Genetic Studies ◽  
Future Studies ◽  
First Time ◽  
Entire Gene

Abstract Considering the high importance of leek as a vegetable crop, this species is rarely an object of molecular genetic studies. In this study, SRAP and ISSR markers were used for the first time to investigate the genetic diversity in Turkish leek accessions together with some morphological characters. The study was conducted in Erciyes University Faculty of Agriculture in 2018–2020. Thirty-seven local varieties were collected from different towns in Turkey. Also, nineteen leek accessions, which originated from different regions of Turkey, had been kindly obtained from Plant Gene Banks, USDA. Seventeen SRAP and three ISSR markers were screened; all of them exposing 137 reproducible bands, of which 114 resulted in polymorphic. Polymorphism information content (PIC) varied between 0.209 and 0.840. Genetic similarities varied from 0.563 to 0.960, with an average of 0.789. In addition to molecular markers, the entire gene pool was morphologically characterized over two-year data. For this purpose, eight characters, which play a key role in leek breeding, were measured. At the end of the study, the analyzes made according to the molecular and morphological data were compared both separately and in combination. In all groupings, it was found remarkable that the accessions numbered 98*3, 40*1, 40*4, and 40*6 were positioned differently from the others. These results have provided important insights into the genetic variability of Turkish leek accessions for the first time. Also, the diversity analysis performed in this study provides valuable information to researchers for future studies.

scholarly journals Morphological and genetic diversity of Senecio vulgaris L. (Asteraceae) in Iran

2021 ◽  
Vol 80 (2) ◽  
Author(s):  
Mostafa Ebadi ◽  
Rosa Eftekharian
Keyword(s):  
Genetic Diversity ◽  
Principal Component ◽  
Morphological Characters ◽  
Mantel Test ◽  
Morphological Data ◽  
Principal Coordinates Analysis ◽  
Cauline Leaf ◽  
Senecio Vulgaris ◽  
Molecular Features ◽  
Principal Coordinates

Senecio vulgaris L., an annual herb belonging to the Asteraceae, is widely distributed in different regions of the world. There is no information on the intraspecific variations of the morphological and molecular features of this species. In the present investigation, we studied the morphological and genetic diversity of 81 accessions of S. vulgaris collected from 10 geographical populations. Eleven inter simple sequence repeat (ISSR) primers were used for the examination of genetic variations among the populations. Analysis of molecular variance (AMOVA) and GST analyses revealed significant differences among the investigated populations. A significant correlation between genetic distance and geographical distance was revealed by the Mantel test. However, reticulation analysis indicated the occurrence of gene flow among most of the populations studied. Principal component analysis (PCA) plot showed that the number of capitula, length of the cauline leaf and plant height were the most variable morphological characters. Principal coordinates analysis (PCoA) plot revealed two groups of populations, according to molecular and morphological data. The results suggested the existence of possible intraspecific taxonomic ranks within this species.

scholarly journals Development and characterization of 24 polymorphic microsatellitelocifor the freshwater fishIchthyoelephas longirostris(Characiformes: Prochilodontidae)

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2419 ◽  
Author(s):  
Ricardo M. Landínez-García ◽  
Edna J. Márquez
Keyword(s):  
Genetic Diversity ◽  
Migratory Species ◽  
Genetic Stock ◽  
Genetic Studies ◽  
Genetic Diversity And Structure ◽  
Polymorphic Microsatellite ◽  
First Time ◽  
Management And Conservation ◽  
Generation Sequencing

The Neotropical freshwater fishIchthyoelephas longirostris(Characiformes: prochilodontidae) is a short-distance migratory species endemic to Colombia. This study developed for the first time a set of 24 polymorphic microsatellitelociby using next-generation sequencing to explore the population genetics of this commercially exploited species. Nineteen of theselociwere used to assess the genetic diversity and structure of 193I. longirostrisin three Colombian rivers of the Magdalena basin. Results showed that a single genetic stock circulates in the Cauca River, whereas other single different genetic stock is present in the rivers Samaná Norte and San Bartolomé-Magdalena. Additionally,I. longirostriswas genetically different among and across rivers. This first insight about the population genetic structure ofI. longirostrisis crucial for monitoring the genetic diversity, the management and conservation of its populations, and complement the genetic studies in Prochilodontidae.

Identification and certification of Bactrian and Arvan camels of Kazakh population using DNA technology// FUNDAMENTAL AND APPLIED SCIENTIFIC RESEARCH: сб.науч.тр./ OEAPS Inc.; редкол.: Флора Бертран (отв.ред.) [и др.]. - Берлин, Германия : OEAPS Inc., 2018. - С. 44-56.

2018 ◽  
Author(s):  
Elmira Kalybaevna Adil’bekova ◽  
Nuradin Alibaev ◽  
Arunas Svitojus
Keyword(s):  
Genetic Resources ◽  
Molecular Genetic ◽  
Structure Identification ◽  
Dna Microsatellites ◽  
Genetic Studies ◽  
Kazakh Population ◽  
Cytogenetic Monitoring ◽  
Dna Technology ◽  
First Time

Kazakhstan is the center where Bactrians and Dromedaries can be raised, and hybridization between them has become widespread. Genetic resources of interspecies camel hybrids, raised in Kazakhstan, are represented by 30 generations.The most highly valuable in the conditions of Central Asia and Kazakhstan are transboundary camel breeds such as Kazakh Bactrian, Turkmen and Kazakh Dromedary, as well as new generations of camels Arada, Bainar and Baitur.At pure breeding of Kazakh Bactrian, genetic peculiarities are not taken into account, in view of the poor knowledge of this issue. To maintain the biodiversity, molecular genetic monitoring of the state of camel populations is required. Cytogenetic monitoring and certification are one of the promising areas for reliable camel genome assessment.Deep determination of Kazakh dairy camelsallele poolwas not carried out in Kazakhstan before. Genetic studies to optimize structure, identification and certification of valuable genotypes, as well as systematization of genetic resources in dairy camel breeding in Kazakhstan are urgent task.The aim of the study is identification, systematization and certification of genetic resources of Bactrian and Dromedary camels of Kazakh population using DNA technology. Genetic analysis of the typing results of the camels studied was carried out by 7 and 8 loci of DNA microsatellites. The material for the studies was DNA samples isolated from histological samples. The microsatellite loci were selected in accordance with the recommendations of the European Society of Genetics (FAO/ISAG).Identification and certification of Arvan and Kazakh Bactrian camels’ genetic resources using the microsatellite analysis of their genotype was carried outfor the first time. Breeding significance of Bactrian and Arvan camels of Kazakh population using DNA technologies was studied and determined. The obtained results of the study allowed evaluate camels at the level of the allelic profile.

Determination of intra-specific genetic variation of Phlomis kurdica and Phlomis oppositiflora and investigation for the hybridity of P. x melitenense (Lamiaceae) by means of molecular markers

Biologia ◽  
2015 ◽  
Vol 70 (9) ◽  
pp. 1159-1171
Author(s):  
Özay Hasan Evren ◽  
Ertuǧrul Yüzbaşıoǧlu ◽  
Mehmet Yaşar Dadandı
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Genetic Distance ◽  
Issr Markers ◽  
Morphological Data ◽  
Quantitative Characters ◽  
Wilks Lambda ◽  
Molecular Aspects ◽  
Species Specific ◽  
Rapd And Issr

Abstract In this study, intra-specific genetic variation and inter-specific genetic relation were investigated among Phlomis oppositiflora, P. kurdica, P. × melitenense (P. kurdica × oppositiflora), P. capitata and P. kurdica × capitata by using RAPD and ISSR markers. The hybridity of P. × melitenense and P. kurdica × capitata samples was also surveyed in terms of morphological and molecular aspects. Except for two, all bands obtained from RAPD (707 bands) and ISSR (651 bands) analyses were polymorphic. The lowest genetic distance values obtained from RAPD and ISSR analyses were 0.0156 (between P. × melitenense and P. kurdica) and 0.0142 (between P. × melitenense and P. kurdica) respectively. The highest genetic distance values obtained from RAPD and ISSR analyses were 0.0866 (between P. kotschyana and P. oppositiflora) and 0.1237 (between P. kotschyana and P. kurdica × capitata) respectively. While P. kurdica indicated the highest genetic diversity (H = 0.1572; I = 0.2646) in RAPD analysis, P. capitata displayed the highest genetic diversity (H = 0.1403; I = 0.2329) in ISSR analysis. AMOVA results showed that 86% and 75% of the total variance resided within groups based on RAPD and ISSR markers, respectively. Based on the RAPD and ISSR results, both P. × melitenense and P. kurdica × capitata samples inherited species specific bands from their parental species, which confirms their hybridity. Although both P. × melitenense and P. kurdica × capitata hybrids showed a morphological mosaic between their parental phenotypes in terms of the majority of the quantitative characters examined, P. × melitenense and P. kurdica × capitata exceeded their parental phenotypes in terms of the three and 11 quantitative characters respectively. MANOVA results from the morphological data showed significant distinction among P. kurdica, P. oppositiflora, P. × melitenense, P. capitata and P. kurdica × capitata (Wilks’ Lambda = 0.003; df = 112; P < 0.01). Average pollen fertilities of P. oppositiflora, P. × melitenense, P. capitata, P. kurdica and P. kurdica × capitata were 93.44%, 68.42%, 93.28 %, 90.12% and 92.77% respectively.

scholarly journals First Descriptions of Larva and Pupa of Bagous claudicans Boheman, 1845 (Curculionidae, Bagoinae) and Systematic Position of the Species Based on Molecular and Morphological Data

Insects ◽  
2019 ◽  
Vol 10 (6) ◽  
pp. 166
Author(s):  
Rafał Gosik ◽  
Miłosz A. Mazur ◽  
Natalia Sawka-Gądek
Keyword(s):  
Feeding Habits ◽  
Morphological Characters ◽  
Systematic Position ◽  
Morphological Data ◽  
Larval Morphology ◽  
Mature Larva ◽  
Larval Stages ◽  
First Time ◽  
Specimen Identification ◽  
First Descriptions

In this paper, the mature larva and pupa of Bagous claudicans are described and illustrated for the first time. Measurements of younger larval instars are also given. The biology of the species is discussed in association with larval morphology and feeding habits. Overall larval and pupal morphological characters of the genus Bagous are presented. Confirmation of the larva identification as Bagous claudicans species was conducted by cytochrome oxidase I (COI) sequencing. DNA barcoding was useful for specimen identification of larval stages. The systematic position of the species within the Bagous collignensis-group, based on morphological and molecular results, is also discussed.

Revision of the genus Trogulus Latreille: the Trogulus coriziformis species-group of the western Mediterranean (Opiliones:Trogulidae)

2008 ◽  
Vol 22 (5) ◽  
pp. 523 ◽  
Author(s):  
Axel L. Schönhofer ◽  
Jochen Martens
Keyword(s):  
Cytochrome B ◽  
Molecular Genetic ◽  
Mediterranean Area ◽  
Molecular Study ◽  
Morphological Characters ◽  
Species Group ◽  
Western Mediterranean ◽  
Morphological Data ◽  
28S Rrna ◽  
Geological Processes

Within the well researched European fauna of harvestmen, the genus Trogulus Latreille exhibits unexpectedly high cryptic diversity. The species’ uniform morphology hinders an exclusively morphological approach to their systematics and taxonomy, and a preliminary molecular study estimated the number of species to be three times higher than currently known. The current study focuses on a clearly defined species-group within Trogulus, combining molecular (~1700 bp 28S rRNA and the cytochrome b gene), distributional, morphometric and morphological data. Relationships are reconstructed using Bayesian inference, maximum parsimony and maximum likelihood and this information is subsequently used to evaluate morphological characters for systematic usability and to identify biogeographical processes leading to speciation events. The Trogulus coriziformis species-group is defined and diagnosed and includes eight species. Three species are redefined: T. coriziformis C. L. Koch, 1839, for which a neotype is designated, and T. aquaticus Simon, 1879 and T. cristatus Simon, 1879 for which lectotypes are designated. Four species are described as new: T. balearicus, sp. nov. from the Balearic Islands, T. huberi, sp. nov. from southern Portugal, T. prietoi, sp. nov. from Andalusia, Spain, and T. pyrenaicus, sp. nov. from the central Pyrenees. Trogulus lusitanicus Giltay, 1931 is used as collective name and probably refers to a composite of species presently difficult to tell apart. Trogulus salfii De Lerma, 1948 is proposed as a synonym for T. coriziformis. Within Trogulus, the molecular genetic data support monophyly and basal placement of the Trogulus coriziformis species-group. The species to differ in external morphology (size, papillation of palps, apophyses of legs, pattern of body papillation, morphometric data), 28S and cytochrome b autapomorphies and to a lesser degree by male genital morphology. The species-group is confined to the western Mediterranean area and its species are allopatrically distributed. Their present distribution corresponds to geological processes in the Miocene and Pliocene indicating that this group of organisms may be of considerable value for further biogeographic studies.

scholarly journals A comparative analysis of genetic diversity in Portuguese grape germplasm from ampelographic collections fit for quality wine production

2016 ◽  
Vol 14 (4) ◽  
pp. e0712 ◽  
Author(s):  
Isaura Castro ◽  
Olinda Pinto-Carnide ◽  
Jesús M. Ortiz ◽  
Vanessa Ferreira ◽  
Juan P. Martín
Keyword(s):  
Genetic Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Morphological Characters ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Molecular Techniques ◽  
Wine Production ◽  
Rapd And Issr ◽  
Simple Sequence

Grapevine cultivars diversity is vast and full of synonyms and homonyms. Up to few decades ago characterization of grapevine was based on morphological characters. In the last decades, molecular markers were developed and have been used as tools to study genetic diversity in a range of different plant species. Fifty-six Portuguese accessions representative of ‘Vinhos Verdes’ and ‘Douro’ Controlled Designations of Origin (DOC) were analysed through DNA fingerprints generated by Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR). The study aimed to compare the effectiveness of RAPD and ISSR molecular techniques in the detection of synonyms, homonyms and misnames. RAPD and ISSR analysis enabled the detection of 36 different band patterns, reducing in about 36% the initial material. Several accessions grown under different names, between and within collections, were confirmed as the same genotype, namely Gouveio/Verdelho, Sousão Douro/Vinhão and Arinto Oeste/Pedernã. Similarly, some homonyms/misnames were also identified, namely within Azal Tinto and Rabigato accessions. RAPD and ISSR markers revealed to be adequate molecular techniques for grapevine varieties fingerprinting with advantages over other molecular procedures, contributing for a good management of grapevine collections.

scholarly journals Analysis of Genetic Diversity in Chrysopogon aciculatus Using Intersimple Sequence Repeat and Sequence-related Amplified Polymorphism Markers

HortScience ◽  
2016 ◽  
Vol 51 (8) ◽  
pp. 972-979 ◽  
Author(s):  
Xinyi Zhang ◽  
Li Liao ◽  
Zhiyong Wang ◽  
Changjun Bai ◽  
Jianxiu Liu
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Molecular Genetic ◽  
Issr Markers ◽  
Data Sets ◽  
Sequence Repeat ◽  
Data Set ◽  
Srap Markers ◽  
Molecular Genetic Diversity ◽  
High Level

Molecular genetic diversity and relationships among 86 Chrysopogon aciculatus (Retz.) Trin. accessions were assessed using intersimple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) markers. Twenty-five ISSR markers generated 283 amplification bands, of which 266 were polymorphic. In addition, 576 polymorphic bands were detected from 627 bands amplified using 30 SRAP primers. Both marker types revealed a high level of genetic diversity, with ISSR markers showing a higher proportion of polymorphic loci (PPL; 94%) than SRAP markers (91.87%). The ISSR and SRAP data were significantly correlated (r = 0.8023). Cluster analysis of the separate ISSR and SRAP data sets clustered the accessions into three groups, which generally were consistent with geographic provenance. Cluster analysis of the combined ISSR and SRAP data set revealed four major groups similar to those based solely on ISSR or SRAP markers. The findings demonstrate that ISSR and SRAP markers are reliable and effective tools for analysis of genetic diversity in C. aciculatus.

scholarly journals СИСТЕМА И КОНСПЕКТ ВИДОВ РОДА PEDICULARIS (OROBANCHACEAE) АЛТАЙСКОЙ ГОРНОЙ СТРАНЫ И ТЯНЬ-ШАНЯ

2016 ◽  
Vol 6 (01) ◽  
pp. 115-166
Author(s):  
P. A. Kosachev
Keyword(s):  
Phylogenetic Tree ◽  
Molecular Genetic ◽  
Tien Shan ◽  
Genetic Studies ◽  
Weak Support ◽  
The Moment ◽  
First Time ◽  
Taxonomic Categories

<span lang="EN-US">The system and conspectus of the genus <em>Pedicularis</em> </span><span lang="EN-US">L. (Orobanchaceae) of </span><span lang="EN-US">Altai and Tien Shan is presented. The system is based of molecular genetic studies of the genus (Ree, 2005; Tkach et al., 2014; own data) and is represented by series that are grouped into sections. The b</span><span lang="EN-US">uilding</span><span lang="EN-US"> </span><span lang="EN-US">of the higher taxonomic categories is not possible at the moment due to the para- </span><span lang="EN-US">or polyphyletic</span><span lang="EN-US"> </span><span lang="EN-US">origin genus of weak support for clads in the phylogenetic tree. Conspectus includes 61 species of the 32 series and 7 sections. 16 species and 1 subspecies of endemic for the territory of the Altai and Tien Shan. For the first time described in one section and 10 series. Three sections published of the lectotypes: <em>Schizocalyx</em> Li, <em>Botryantha</em> Li, <em>Rhizophyllum</em> Li. Clarified the amount and distribution of species, series, sections. Displaying Altai and Tien Shan origin some series (ser. <em>Abrotanifoliae, Amoenae, Achilleifolia, Uliginosae, Physocalyces, Macrochilae, Platyrhynchae</em>), the Tien-Shan (<em>Semenowianae, Pycnanthae, Maximowiczianae, Pubiflorae</em>), Altai (<em>Brachystaches, Elatae</em>).</span>

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