Angiopoietins' (Ang-1 and Ang-2) mRNA Expression Levels in Lung Tissue of IPF Patients.

2009 ◽  
Author(s):  
A Proklou ◽  
KM Antoniou ◽  
R Lymbouridou ◽  
G Soufla ◽  
K Karagiannis ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Lung Tissue ◽  
Expression Levels ◽  
Mrna Expression Levels

Effect of cigarette smoke on TNF-a and IL-1b mRNA expression in lung tissues and cells of rats

2015 ◽  
Author(s):  
Mingli Ji ◽  
Yuxia Wang ◽  
Xiaopeng Li ◽  
Zhibi Qian
Keyword(s):  
Carbon Dioxide ◽  
Mrna Expression ◽  
Cigarette Smoke ◽  
Lung Tissue ◽  
Control Group ◽  
Expression Levels ◽  
Arterial Partial Pressure ◽  
Experimental Group ◽  
Mrna Expression Levels ◽  
Pro Inflammatory Cytokines

We investigated lung tissue expression of the pro-inflammatory cytokines TNF-a and IL-1b in response to cigarette smoke exposure and the ensuing effects on arterial oxygen and carbon dioxide as indices of respiratory function. Experimental group rats were exposed to cigarette smoke twice daily (30 min per exposure) for 28 consecutive days. Arterial partial pressure of oxygen (PO2), arterial partial pressure of carbon dioxide (PCO2), and both mRNA and protein expression levels of TNF-a and IL-1b were compared to a control group. Contents of TNF-a and IL-1b in bronchoalveolar lavage fluid (BALF) and lung homogenate were detected by enzyme linked immunosorbent assays while TNF-a mRNA and IL-1b mRNA expression levels in lung tissue were detected by reverse transcription-polymerase chain reaction. Arterial PO2 was significantly lower in the experimental group than the control group, while the arterial PCO2 was significantly higher. BALF levels of TNF-a and IL-1b were significantly higher in the experimental group than the control group, as were TNF-a mRNA and IL-1b mRNA expression levels in lung tissue. Cigarette smoke may activate inflammatory cells in the pulmonary circulation and increase the expression of the pro-inflammatory cytokines TNF-a and IL-1b in lung tissue, leading to lung injury and respiratory dysfunction.

scholarly journals The role of CXCR3 and its ligands expression in Brucellar spondylitis

2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Xin Hu ◽  
Xiaoqian Shang ◽  
Liang Wang ◽  
Jiahui Fan ◽  
Yue Wang ◽  
...  
Keyword(s):  
Protein Expression ◽  
Mrna Expression ◽  
Mononuclear Cells ◽  
Healthy Controls ◽  
Inflammatory Infiltration ◽  
Peripheral Blood Mononuclear ◽  
Expression Levels ◽  
Inflammatory Damage ◽  
Ifn Γ ◽  
Mrna Expression Levels

Abstract Aim Brucellar spondylitis (BS) is one of the most serious complications of brucellosis. CXCR3 is closely related to the severity of disease infection. This research aimed to study the degree of BS inflammatory damage through analyzing the expression levels of CXCR3 and its ligands (CXCL9 and CXCL10) in patients with BS. Methods A total of 29 BS patients and 15 healthy controls were enrolled. Real-Time PCR was used to detect the mRNA expression levels of IFN-γ, CXCR3, CXCL9 and CXCL10 in peripheral blood mononuclear cells (PBMCs) of BS patients and healthy controls. Hematoxylin-Eosin staining was used to show the pathological changes in BS lesion tissues. Immunohistochemistry staining was used to show the protein expression levels of Brucella-Ab, IFN-γ, CXCR3, CXCL9 and CXCL10 in BS lesion tissues. At the same time, ELISA was used to detect the serum levels of IFN-γ, CXCL9 CXCL10 and autoantibodies against CXCR3 in patients with BS. Results In lesion tissue of BS patients, it showed necrosis of cartilage, acute or chronic inflammatory infiltration. Brucella-Ab protein was abundantly expressed in close lesion tissue. And the protein expression levels of IFN-γ, CXCR3 and CXCL10 were highly expressed in close lesion tissue and serum of BS patients. At the same time, the mRNA expression levels of IFN-γ, CXCR3 and CXCL10 in PBMCs of BS patients were significantly higher than those in controls. Conclusion In our research, the expression levels of IFN-γ, CXCR3 and its ligands were significantly higher than those in controls. It suggested that high expression levels of IFN-γ, CXCR3 and its ligands indicated a serious inflammatory damage in patients with BS.

Unilateral labyrinthectomy induced changes on GDNF receptor complex proteins in the rat medial vestibular nuclei

2007 ◽  
Vol 16 (4-5) ◽  
pp. 171-177
Author(s):  
Adrian Lozada ◽  
Kaj Karlstedt ◽  
Pertti Panula ◽  
Antti A. Aarnisalo
Keyword(s):  
Mrna Expression ◽  
Vestibular Nucleus ◽  
Medial Vestibular Nucleus ◽  
Receptor Complex ◽  
Trophic Effect ◽  
Expression Levels ◽  
Protein Levels ◽  
Unilateral Labyrinthectomy ◽  
Ganglion Neurons ◽  
Mrna Expression Levels

In the auditory periphery, GDNF has been shown to have a trophic effect to spiral ganglion neurons, both during development and in adult animals. We have studied the effect of unilateral labyrinthectomy (UL) on protein levels and expression of GDNF multicomponent receptor complex: the ret tyrosine kinase and coreceptor GFRα-1 in the medial vestibular nucleus of the adult rat. GFRα-1 protein levels display an increasing trend in ipsilateral medial vestibular nucleus culminating at 48 h post UL. On the other hand, GFRα-1 mRNA expression levels in ipsi- and contralateral medial vestibular nucleus show a steadily decreasing trend that is significant at 1 week post-lesion. Protein levels for c-Ret isoforms also show an initial bilateral decreasing trend that ceases at 48 h in ipsilateral medial vestibular nucleus but persists on the contralateral side. c-Ret mRNA expression levels show a significant decrease at 4 h post UL followed by another significant decrease 1 week post UL. Our data would suggest that neurotrophins belonging to the GDNF family are involved in this model of post-lesional CNS plasticity.

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