Disruptive Effect of LAM-Derived Smooth Muscle Cells on Human Lung Progenitors Is Dependent on Expression of Translationally Controlled Tumour Protein

2019 ◽  
Author(s):  
M. Ho ◽  
M. Ho ◽  
L.M. Julian ◽  
W.L. Stanford ◽  
D.J. Stewart
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Human Lung ◽  
Muscle Cells ◽  
Disruptive Effect ◽  
Lung Progenitors

POSTNATAL FORMATION AND OBLITERATION OF ARTERIAL ANASTOMOSES IN THE HUMAN LUNG

PEDIATRICS ◽  
1969 ◽  
Vol 43 (6) ◽  
pp. 971-979
Author(s):  
Bengt Robertson
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Human Lung ◽  
Fibrous Tissue ◽  
Muscle Cells ◽  
Full Term ◽  
Infants And Children ◽  
Bronchial Arteries ◽  
The Individual ◽  
Neonatal Infants

The incidence and structure of arterial bronchopulmonary anastomoses and pulmobronchial arteries were studied by microangiographic and histologic techniques in material from 37 immature, premature, and full-term neonatal infants and 15 infants and children varying in age from 3 weeks to 4 years, 7 months. The number of arterial bronchopulmonary anastomoses was found to increase with postnatal age. The opposite was true for the pulmobronchial arteries, and some anastomoses were probably formed from pulmobronchial arteries which established precapillary communication with adjacent branches of true bronchial arteries. From the age of 2½ months, many anastomoses are narrowed by intimal smooth muscle cells; and, from the age of 7 months, some anastomoses are completely obliterated by smooth muscle cells and fibrous tissue. The majority of the arterial bronchopulmonary anastomoses probably become obliterated as the individual approaches adulthood.

Human lung mast cells modulate the functions of airway smooth muscle cells in asthma

Allergy ◽  
2011 ◽  
Vol 66 (9) ◽  
pp. 1231-1241 ◽  
Author(s):  
H. Alkhouri ◽  
F. Hollins ◽  
L. M. Moir ◽  
C. E. Brightling ◽  
C. L. Armour ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Mast Cells ◽  
Smooth Muscle Cells ◽  
Airway Smooth Muscle ◽  
Human Lung ◽  
Muscle Cells ◽  
Airway Smooth Muscle Cells

scholarly journals Transcriptional characterisation of human lung cells identifies novel mesenchymal lineage markers

2019 ◽  
Vol 55 (1) ◽  
pp. 1900746 ◽  
Author(s):  
Soula Danopoulos ◽  
Soumyaroop Bhattacharya ◽  
Thomas J. Mariani ◽  
Denise Al Alam
Keyword(s):  
Smooth Muscle ◽  
Endothelial Cell ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Human Lung ◽  
Muscle Cells ◽  
Cell Populations ◽  
Marker Genes ◽  
Sequencing Analysis ◽  
Human Lung Cells

RationaleThe lung mesenchyme gives rise to multiple distinct lineages of cells in the mature respiratory system, including smooth muscle cells of the airway and vasculature. However, a thorough understanding of the specification and mesenchymal cell diversity in the human lung is lacking.MethodsWe completed single-cell RNA sequencing analysis of fetal human lung tissues. Canonical correlation analysis, clustering, cluster marker gene identification and t-distributed stochastic neighbour embedding representation was performed in Seurat. Cell populations were annotated using ToppFun. Immunohistochemistry and in situ hybridisation were used to validate spatiotemporal gene expression patterns for key marker genes.ResultsWe identified molecularly distinct populations representing “committed” fetal human lung endothelial cells, pericytes and smooth muscle cells. Early endothelial lineages expressed “classic” endothelial cell markers (platelet endothelial cell adhesion molecule/CD31 and claudin 5), while pericytes expressed platelet-derived growth factor receptor-β, Thy-1 membrane glycoprotein and basement membrane molecules (collagen IV, laminin and proteoglycans). We observed a large population of “nonspecific” human lung mesenchymal progenitor cells characterised by expression of collagen I and multiple elastin fibre genes (ELN, MFAP2 and FBN1). We closely characterised the diversity of mesenchymal lineages defined by α2-smooth muscle actin (ACTA2) expression. Two cell populations, with the highest levels of ACTA2 transcriptional activity, expressed unique sets of markers associated with airway or vascular smooth muscle cells. Spatiotemporal analysis of these marker genes confirmed early and persistent spatial specification of airway (HHIP, MYLK and IGF1) and vascular (NTRK3 and MEF2C) smooth muscle cells in the developing human lung.ConclusionOur data suggest that specification of distinct airway and vascular smooth muscle cell phenotypes is established early in development and can be identified using the markers we provide.

Immunogold localization of HMB-45 antigen in pulmonary lymphangiomyomatosis

1995 ◽  
Vol 53 ◽  
pp. 998-999
Author(s):  
J.M. Minda ◽  
E. Dessy ◽  
G. G. Pietra
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Room Temperature ◽  
Osmium Tetroxide ◽  
Muscle Cells ◽  
Ultrastructural Localization ◽  
Reproductive Age ◽  
Thin Sections ◽  
Smooth Muscle Proliferation ◽  
Hmb 45

Pulmonary lymphangiomyomatosis (PLAM) is a rare disease occurring exclusively in women of reproductive age. It involves the lungs, lymph nodes and lymphatic ducts. In the lungs, it is characterized by the proliferation of smooth muscle cells around lymphatics in the bronchovascular bundles, lobular septa and pleura The nature of smooth muscle proliferation in PLAM is still unclear. Recently, reactivity of the smooth muscle cells for HMB-45, a melanoma-related antigen has been reported by immunohistochemistry. The purpose of this study was the ultrastructural localization of HMB-45 immunoreactivity in these cells using gold-labeled antibodies.Lung tissue from three cases of PLAM, referred to our Institution for lung transplantation, was embedded in either Poly/Bed 812 post-fixed in 1% osmium tetroxide, or in LR White, without osmication. For the immunogold technique, thin sections were placed on Nickel grids and incubated with affinity purified, monoclonal anti-melanoma antibody HMB-45 (1:1) (Enzo Diag. Co) overnight at 4°C. After extensive washing with PBS, grids were treated with Goat-anti-mouse-IgG-Gold (5nm) (1:10) (Amersham Life Sci) for 1 hour, at room temperature.

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