Hyphomycetes from aquatic habitats in Southern China: Species of Curvularia (Pleosporaceae) and Phragmocephala (Melannomataceae)

Phytotaxa ◽  
2015 ◽  
Vol 226 (3) ◽  
pp. 201 ◽  
Author(s):  
HONG -YAN SU ◽  
Dhanushka Udayanga ◽  
Zong-long Luo ◽  
Dimuthu Manamgoda ◽  
Yong-Chang Zhao ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Sequence Data ◽  
Southern China ◽  
Freshwater Ecosystems ◽  
Aquatic Hyphomycetes ◽  
Dna Sequence Data ◽  
Freshwater Streams ◽  
The Family ◽  
Freshwater Habitats ◽  
First Time

Aquatic hyphomycetes are a diverse, polyphyletic group of asexually reproducing fungi involved in the decomposition of litter in freshwater ecosystems. Curvularia eragrostidis, C. verruculosa and Phragmocephala atra were identified from submerged wood collected from freshwater streams in Yunnan Province, Southwestern China. They were characterised based on morphology and LSU, ITS and SSU sequence data. Phylogenetic analysis of LSU sequences placed the isolates within the order Pleosporales. Curvularia eragrostidis and C. verruculosa are reported from freshwater habitats for the first time. An epitype is designated for Curvularia verruculosa. This is the first phylogenetic placement of the genus Phragmocephala in the family Melanommataceae in Dothideomycetes, providing new DNA sequence data. A new species, Phragmocephala garethjonesii is introduced based on DNA sequence data and morphology. Descriptions and illustrations are provided for the species with notes on their taxonomy and phylogeny.

scholarly journals Sequences of the second internal transcribed spacer of ribosomal DNA for three species of Trichostrongylus (Nematoda: Trichostrongylidae) from sheep in Russia

2007 ◽  
Vol 44 (2) ◽  
pp. 43-46 ◽  
Author(s):  
D. Kuznetsov ◽  
N. Kuznetsova
Keyword(s):  
Dna Sequence ◽  
Intraspecific Variation ◽  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Moscow Region ◽  
Dna Sequence Data ◽  
Interspecific Differences ◽  
Second Internal Transcribed Spacer ◽  
First Time

AbstractFor the first time, DNA sequence data were obtained for three species of Trichostrongylus from Russia. Internal transcribed spacer (ITS-2) of ribosomal DNA was sequenced for T. axei, T. colubriformis and T. probolurus from sheep from the Moscow region. ITS-2 rDNA length was estimated as 238 nucleotides for T. colubriformis and T. probolurus and 237 nucleotides for T. axei. The G+C content of the ITS-2 sequences of T. colubriformis, T. axei and T. probolurus were 31 %, 32 % and 34 % respectively. The level of interspecific differences in ITS-2 of rDNA of T. axei, T. probolurus and T. colubriformis ranged from 3 to 4 %. The ITS-2 sequences from the Russian specimens were compared with those of T. axei, T. probolurus and T. colubriformis from Australia and Germany. Intraspecific variation ranged from 0 % in T. colubriformis to 3.0 % in T. axei.

Immature stages, host plants and natural enemies of Henosepilachna implicata (Mulsant) (Coleoptera: Coccinellidae) with DNA sequence data and a new synonym and notes on some Indian species of Epilachnini

Zootaxa ◽  
2021 ◽  
Vol 4970 (3) ◽  
pp. 533-546
Author(s):  
J. POORANI ◽  
R. G. BOOTH ◽  
R. GANDHI GRACY ◽  
C. ANURADHA ◽  
R. THANIGAIRAJ ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Natural Enemies ◽  
Host Plants ◽  
Sequence Data ◽  
Indian Species ◽  
Life Stages ◽  
Important Species ◽  
Dna Sequence Data ◽  
New Synonym ◽  
First Time

Life stages of Henosepilachna implicata (Mulsant), an economically important species of Epilachnini in India, are documented and illustrated. Mitochondrial DNA sequence data is provided for the first time for H. implicata with additional details on its host plants, distribution, and natural enemies. Its similarities and differences with other common pestiferous Henosepilachna spp. in India such as H. vigintioctopunctata (F.), H. septima (Dieke) and H. pusillanima (Mulsant) are discussed. Epilachna circularis Korschefsky, 1933 is found to be conspecific with H. implicata and is reduced to a junior synonym of the latter (new synonym). Notes are given on the distribution and natural enemies of some other species of Epilachnini of the Indian region.  

scholarly journals The Genera of Fungi – G6: Arthrographis, Kramasamuha, Melnikomyces, Thysanorea, and Verruconis

2020 ◽  
Vol 6 (1) ◽  
pp. 1-24
Author(s):  
M. Hernández-Restrepo ◽  
A. Giraldo ◽  
R. van Doorn ◽  
M.J. Wingfield ◽  
J.Z. Groenewald ◽  
...  
Keyword(s):  
New Species ◽  
Dna Sequence ◽  
Type Species ◽  
New Genus ◽  
Sequence Data ◽  
Dna Sequence Data ◽  
Two New Species ◽  
First Time

The Genera of Fungi series, of which this is the sixth contribution, links type species of fungal genera to their morphology and DNA sequence data. Five genera of microfungi are treated in this study, with new species introduced in Arthrographis, Melnikomyces, and Verruconis. The genus Thysanorea is emended and two new species and nine combinations are proposed. Kramasamuha sibika, the type species of the genus, is provided with DNA sequence data for first time and shown to be a member of Helminthosphaeriaceae (Sordariomycetes). Aureoconidiella is introduced as a new genus representing a new lineage in the Dothideomycetes.

Perssoniella and the genera of Schistochilaceae: a new classification based on molecular phylogenies

2010 ◽  
Vol 23 (4) ◽  
pp. 229 ◽  
Author(s):  
Xiaolan He ◽  
David Glenny
Keyword(s):  
Dna Sequence ◽  
New Caledonia ◽  
Sequence Data ◽  
Strong Support ◽  
Morphological Characters ◽  
Monotypic Genus ◽  
New Classification ◽  
Dna Sequence Data ◽  
The Family ◽  
Molecular Phylogenies

The monotypic genus Perssoniella with P. vitreocincta Herzog, endemic to New Caledonia, possesses a series of unique morphological characters and it has been assumed that the genus, assigned to the family Perssoniellaceae and suborder Perssoniellineae, is very isolated but sister to the family Schistochilaceae. The systematic identity of Perssoniella vitreocincta was studied using DNA sequence data for the chloroplast rbcL, rps4 and trnL-F regions. Our analyses placed Perssoniella vitreocincta within the family Schistochilaceae, and within Schistochila itself, with strong support. It suggests that retaining Perssoniella as an independent genus is untenable and we transfer it to the genus Schistochila. Our results indicate that Perssoniella vitreocincta is not an archaic species, as presupposed earlier. The differentiating characters in Perssoniella are mostly probably later derived, rather than ancestral. Our analyses also placed Pachyschistochila and Paraschistochila within Schistochila, again with strong support. We also transfer these two genera to Schistochila.

scholarly journals Two new asexual genera and six new asexual species in the family Microthyriaceae (Dothideomycetes, Ascomycota) from China

MycoKeys ◽  
2021 ◽  
Vol 85 ◽  
pp. 1-30
Author(s):  
Min Qiao ◽  
Hua Zheng ◽  
Ji-Shu Guo ◽  
Rafael F. Castañeda-Ruiz ◽  
Jian-Ping Xu ◽  
...  
Keyword(s):  
New Taxa ◽  
Dna Sequences ◽  
Sequence Data ◽  
Southern China ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Aquatic Hyphomycetes ◽  
Internal Transcribed Spacers ◽  
The Family

The family Microthyriaceae is represented by relatively few mycelial cultures and DNA sequences; as a result, the taxonomy and classification of this group of organisms remain poorly understood. During the investigation of the diversity of aquatic hyphomycetes from southern China, several isolates were collected. These isolates were cultured and sequenced and a BLAST search of its LSU sequences against data in GenBank revealed that the closest related taxa are in the genus Microthyrium. Phylogenetic analyses, based on the combined sequence data from the internal transcribed spacers (ITS) and the large subunit (LSU), revealed that these isolates represent eight new taxa in Microthyriaceae, including two new genera, Antidactylariagen. nov. and Isthmomycesgen. nov. and six new species, Antidactylaria minifimbriatasp. nov., Isthmomyces oxysporussp. nov., I. dissimilissp. nov., I. macrosporussp. nov., Triscelophorus anisopterioideussp. nov. and T. sinensissp. nov. These new taxa are described, illustrated for their morphologies and compared with similar taxa. In addition, two new combinations are proposed in this family.

scholarly journals On the monophyly of subfamily Tectarioideae (Polypodiaceae) and the phylogenetic placement of some associated fern genera 

Phytotaxa ◽  
2014 ◽  
Vol 164 (1) ◽  
pp. 1 ◽  
Author(s):  
FA-GUO WANG ◽  
SAM BARRATT ◽  
WILFREDO FALCÓN ◽  
MICHAEL F. FAY ◽  
SAMULI LEHTONEN ◽  
...  
Keyword(s):  
New Caledonia ◽  
Sequence Data ◽  
Intergenic Spacer ◽  
Plastid Dna ◽  
Dna Sequence Data ◽  
Phylogenetic Placement ◽  
The Past ◽  
The Family ◽  
The Caribbean ◽  
First Time

The fern genus Tectaria has generally been placed in the family Tectariaceae or in subfamily Tectarioideae (placed in Dennstaedtiaceae, Dryopteridaceae or Polypodiaceae), both of which have been variously circumscribed in the past. Here we study for the first time the phylogenetic relationships of the associated genera Hypoderris (endemic to the Caribbean), Cionidium (endemic to New Caledonia) and Pseudotectaria (endemic to Madagascar and Comoros) using DNA sequence data. Based on a broad sampling of 72 species of eupolypods I (= Polypodiaceae sensu lato) and three plastid DNA regions (atpA, rbcL and the trnL-F intergenic spacer) we were able to place the three previously unsampled genera. Our results show that Cionidium, like Ctenitopsis, Fadyenia, Hemigramma and Quercifilix, is embedded in Tectaria, and the monophyly of Tectaria is therefore corroborated only if these segregate genera are included. Hypoderris is sister to Tectaria brauniana and together they are sister to Triplophyllum, which was found to be monophyletic. Despite their morphological similarity with Tectaria, the genera Pleocnemia and Pseudotectaria were placed in Dryopteridoideae. Polypodiaceae subfamily Tectarioideae (former family Tectariaceae) is hereby defined to include Arthropteris, Hypoderris, Pteridrys, Tectaria and Triplophyllum. Aenigmopteris may also belong here, but this genus remains unsampled.

Delayed germination of basidiospores in temperate species of Crepidotus (Fr.) Staude

2002 ◽  
Vol 80 (3) ◽  
pp. 280-287 ◽  
Author(s):  
M C Aime ◽  
O K Miller Jr.
Keyword(s):  
Dna Sequence ◽  
Sequence Data ◽  
Abiotic Factors ◽  
Large Subunit ◽  
Early Spring ◽  
Single Spore ◽  
Dna Sequence Data ◽  
Delayed Germination ◽  
First Time ◽  
Ribosomal Dna Sequence

Delayed basidiospore germination (endogenous dormancy) for a number of species of Crepidotus (Agaricales: Crepidotaceae) is reported for the first time. Ninety percent of recovered single spore isolates germinated between 18 and 36 weeks after collection; average germination was 25 weeks after collection. The period in which 90% of germinations occurred was between 17 February and 19 April, with 50% of recovered isolates germinating in March. Many abiotic factors were experimentally manipulated in an effort to reduce or alter the necessary incubation period without effect. The latent period was consistent for a given collection, with the majority of recovered isolates from fall-fruiting collections germinating during early spring, regardless of whether spores were plated immediately after harvesting or stored for one to several months prior to plating. The identity of the cultures derived from delayed germination was confirmed by DNA sequencing.Key words: basidiospore, dormancy, single spore isolates, Crepidotaceae, large subunit ribosomal DNA sequence data, phylogeny.

scholarly journals Unravelling unexplored diversity of cercosporoid fungi (Mycosphaerellaceae, Mycosphaerellales, Ascomycota) in tropical Africa

MycoKeys ◽  
2021 ◽  
Vol 81 ◽  
pp. 69-138
Author(s):  
Yalemwork Meswaet ◽  
Ralph Mangelsdorff ◽  
Nourou S. Yorou ◽  
Meike Piepenbring
Keyword(s):  
West Africa ◽  
Dna Sequence ◽  
Vigna Unguiculata ◽  
Sequence Data ◽  
Morphological Characteristics ◽  
Molecular Sequence Data ◽  
Dna Sequence Data ◽  
Wide Range ◽  
Cercosporoid Fungi ◽  
First Time

Cercosporoid fungi (Mycosphaerellaceae, Mycosphaerellales, Ascomycota) are one of the largest and most diverse groups of hyphomycetes causing a wide range of diseases of economically important plants as well as of plants in the wild. Although more than 6000 species are known for this group, the documentation of this fungal group is far from complete. Especially in the tropics, the diversity of cercosporoid fungi is poorly known. The present study aims to identify and characterise cercosporoid fungi collected on host plants belonging to Fabaceae in Benin, West Africa. Information on their morphology, host species and DNA sequence data (18S rDNA, 28S rDNA, ITS and tef1) is provided. DNA sequence data were obtained by a simple and non-culture-based method for DNA isolation which has been applied for cercosporoid fungi for the first time in the context of the present study. Among the loci used for the phylogenetic analysis, tef1 provided the best resolution together with the multigene dataset. Species delimitation in many cases, however, was only possible by combining molecular sequence data with morphological characteristics. Based on forty specimens recently collected in Benin, 18 species are presented with morphological descriptions, illustrations and sequence data. Among these, six species in the genus Cercospora and two species in Pseudocercospora are proposed as species new to science. The newly described species are Cercospora (C.) beninensis on Crotalaria macrocalyx, C. parakouensis on Desmodium tortuosum, C. rhynchophora on Vigna unguiculata, C. vignae-subterraneae on Vigna subterranea, C. tentaculifera on Vigna unguiculata, C. zorniicola on Zornia glochidiata, Pseudocercospora sennicola on Senna occidentalis and Pseudocercospora tabei on Vigna unguiculata. Eight species of cercosporoid fungi are reported for Benin for the first time, three of them, namely C. cf. canscorina, C. cf. fagopyri and C. phaseoli-lunati are new for West Africa. The presence of two species of cercosporoid fungi on Fabaceae previously reported from Benin, namely Nothopassalora personata and Passalora arachidicola, is confirmed.

scholarly journals Ecology and seasonality of Pseudo-nitzschia species (Bacillariophyceae) in the northwestern Adriatic Sea over a 30-years period (1988–2020)

2021 ◽  
Vol 22 (3) ◽  
pp. 505
Author(s):  
SONIA GIULIETTI ◽  
TIZIANA ROMAGNOLI ◽  
ALESSANDRA CAMPANELLI ◽  
CECILIA TOTTI ◽  
STEFANO ACCORONI
Keyword(s):  
Dna Sequence ◽  
Dna Sequences ◽  
Species Complex ◽  
Adriatic Sea ◽  
Sequence Data ◽  
Coastal Station ◽  
Dna Sequence Data ◽  
The Mean ◽  
First Time ◽  
North Western

The ecology and seasonality of Pseudo-nitzschia species and their contribution to phytoplankton community were analysed for the first time at the coastal station of the LTER-Senigallia-Susak transect (north-western Adriatic Sea) from 1988 to 2020. Species composition was addressed using DNA sequence data obtained from 106 monoclonal strains isolated from January 2018 to January 2020. The mean annual cycle of total phytoplankton in the study period (Feb 1988–Jan 2020) showed maximum abundances in winter followed by other peaks in spring and autumn. Diatoms were the main contributors in terms of abundance during the winter and the spring blooms. The autumn peak was due to phytoflagellates and diatoms. In summer phytoflagellates dominated the community, followed by diatoms and dinoflagellates, which in this season reached their annual maximum. Pseudo-nitzschia spp. represented on average 0.4–17.6% of diatom community, but during their blooms they could reach up to up to 90% of the total diatom abundances with 106 cells l-1. By LM, six different taxa were recognized: Pseudo-nitzschia cf. delicatissima and P. cf. pseudodelicatissima were the most abundant, followed by P. cf. fraudulenta, P. pungens, P. multistriata and P. cf. galaxiae. P. cf. fraudulenta and P. pungens were indicator taxa of winter. P. cf. delicatissima and P. cf. pseudodelicatissima were spring and summer taxa, respectively. P. galaxiae showed maximum abundances in autumn. DNA sequences revealed the presence of two species belonging to the ’P. seriata group’ (i.e. P. fraudulenta and P. pungens) and four species belonging to the ‘P. delicatissima group’ (P. calliantha and P. mannii within the P. pseudodelicatissima species complex, and P. delicatissima and P. cf. arenysensis within the P. delicatissima species complex). The presence of several cryptic and pseudo-cryptic species highlights the need to combine LM observations with DNA sequence data when the ecology of Pseudo-nitzschia is investigated. 

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