Immature stages, host plants and natural enemies of Henosepilachna implicata (Mulsant) (Coleoptera: Coccinellidae) with DNA sequence data and a new synonym and notes on some Indian species of Epilachnini

Zootaxa ◽  
2021 ◽  
Vol 4970 (3) ◽  
pp. 533-546
Author(s):  
J. POORANI ◽  
R. G. BOOTH ◽  
R. GANDHI GRACY ◽  
C. ANURADHA ◽  
R. THANIGAIRAJ ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Natural Enemies ◽  
Host Plants ◽  
Sequence Data ◽  
Indian Species ◽  
Life Stages ◽  
Important Species ◽  
Dna Sequence Data ◽  
New Synonym ◽  
First Time

Life stages of Henosepilachna implicata (Mulsant), an economically important species of Epilachnini in India, are documented and illustrated. Mitochondrial DNA sequence data is provided for the first time for H. implicata with additional details on its host plants, distribution, and natural enemies. Its similarities and differences with other common pestiferous Henosepilachna spp. in India such as H. vigintioctopunctata (F.), H. septima (Dieke) and H. pusillanima (Mulsant) are discussed. Epilachna circularis Korschefsky, 1933 is found to be conspecific with H. implicata and is reduced to a junior synonym of the latter (new synonym). Notes are given on the distribution and natural enemies of some other species of Epilachnini of the Indian region.  

scholarly journals Sequences of the second internal transcribed spacer of ribosomal DNA for three species of Trichostrongylus (Nematoda: Trichostrongylidae) from sheep in Russia

2007 ◽  
Vol 44 (2) ◽  
pp. 43-46 ◽  
Author(s):  
D. Kuznetsov ◽  
N. Kuznetsova
Keyword(s):  
Dna Sequence ◽  
Intraspecific Variation ◽  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Moscow Region ◽  
Dna Sequence Data ◽  
Interspecific Differences ◽  
Second Internal Transcribed Spacer ◽  
First Time

AbstractFor the first time, DNA sequence data were obtained for three species of Trichostrongylus from Russia. Internal transcribed spacer (ITS-2) of ribosomal DNA was sequenced for T. axei, T. colubriformis and T. probolurus from sheep from the Moscow region. ITS-2 rDNA length was estimated as 238 nucleotides for T. colubriformis and T. probolurus and 237 nucleotides for T. axei. The G+C content of the ITS-2 sequences of T. colubriformis, T. axei and T. probolurus were 31 %, 32 % and 34 % respectively. The level of interspecific differences in ITS-2 of rDNA of T. axei, T. probolurus and T. colubriformis ranged from 3 to 4 %. The ITS-2 sequences from the Russian specimens were compared with those of T. axei, T. probolurus and T. colubriformis from Australia and Germany. Intraspecific variation ranged from 0 % in T. colubriformis to 3.0 % in T. axei.

scholarly journals The Genera of Fungi – G6: Arthrographis, Kramasamuha, Melnikomyces, Thysanorea, and Verruconis

2020 ◽  
Vol 6 (1) ◽  
pp. 1-24
Author(s):  
M. Hernández-Restrepo ◽  
A. Giraldo ◽  
R. van Doorn ◽  
M.J. Wingfield ◽  
J.Z. Groenewald ◽  
...  
Keyword(s):  
New Species ◽  
Dna Sequence ◽  
Type Species ◽  
New Genus ◽  
Sequence Data ◽  
Dna Sequence Data ◽  
Two New Species ◽  
First Time

The Genera of Fungi series, of which this is the sixth contribution, links type species of fungal genera to their morphology and DNA sequence data. Five genera of microfungi are treated in this study, with new species introduced in Arthrographis, Melnikomyces, and Verruconis. The genus Thysanorea is emended and two new species and nine combinations are proposed. Kramasamuha sibika, the type species of the genus, is provided with DNA sequence data for first time and shown to be a member of Helminthosphaeriaceae (Sordariomycetes). Aureoconidiella is introduced as a new genus representing a new lineage in the Dothideomycetes.

Association of larvae and adults of Mexican species of Macrelmis (Coleoptera: Elmidae): a preliminary analysis using DNA sequences

Zootaxa ◽  
2012 ◽  
Vol 3361 (1) ◽  
pp. 56-62 ◽  
Author(s):  
JOSEFINA CURIEL ◽  
JUAN J. MORRONE
Keyword(s):  
Dna Sequence ◽  
Dna Sequences ◽  
Preliminary Analysis ◽  
Sequence Data ◽  
Strong Association ◽  
Life Stages ◽  
Adult Morphology ◽  
Dna Sequence Data ◽  
Mexican Species ◽  
Major Impediment

Insect life stages are known imperfectly in many cases, and classifications are usually based on adult morphology. This isunfortunate as information on other life stages may be useful for biomonitoring. The major impediment to using elmid(Coleoptera) larvae for freshwater biomonitoring is the lack of larval descriptions and illustrations. Reliable molecular proto-cols may be used to associate larvae and adults. After adults of seven species of Mexican Macrelmis were identified morpho-logically, seven larval specimens were associated to them based on two gene fragments: Cox1 and Cob. The phylogeneticanalysis allowed identifying the larval specimens as Macrelmis leonilae, M. scutellaris, M. species 7, M. species 10, and M.species 11. Two species based on adults associated uncertainly with one larva, and one larva did not match with any adult. Adult/larval association in elmids using DNA sequence data seems to be promising in terms of speed and reliability.

Delayed germination of basidiospores in temperate species of Crepidotus (Fr.) Staude

2002 ◽  
Vol 80 (3) ◽  
pp. 280-287 ◽  
Author(s):  
M C Aime ◽  
O K Miller Jr.
Keyword(s):  
Dna Sequence ◽  
Sequence Data ◽  
Abiotic Factors ◽  
Large Subunit ◽  
Early Spring ◽  
Single Spore ◽  
Dna Sequence Data ◽  
Delayed Germination ◽  
First Time ◽  
Ribosomal Dna Sequence

Delayed basidiospore germination (endogenous dormancy) for a number of species of Crepidotus (Agaricales: Crepidotaceae) is reported for the first time. Ninety percent of recovered single spore isolates germinated between 18 and 36 weeks after collection; average germination was 25 weeks after collection. The period in which 90% of germinations occurred was between 17 February and 19 April, with 50% of recovered isolates germinating in March. Many abiotic factors were experimentally manipulated in an effort to reduce or alter the necessary incubation period without effect. The latent period was consistent for a given collection, with the majority of recovered isolates from fall-fruiting collections germinating during early spring, regardless of whether spores were plated immediately after harvesting or stored for one to several months prior to plating. The identity of the cultures derived from delayed germination was confirmed by DNA sequencing.Key words: basidiospore, dormancy, single spore isolates, Crepidotaceae, large subunit ribosomal DNA sequence data, phylogeny.

scholarly journals Unravelling unexplored diversity of cercosporoid fungi (Mycosphaerellaceae, Mycosphaerellales, Ascomycota) in tropical Africa

MycoKeys ◽  
2021 ◽  
Vol 81 ◽  
pp. 69-138
Author(s):  
Yalemwork Meswaet ◽  
Ralph Mangelsdorff ◽  
Nourou S. Yorou ◽  
Meike Piepenbring
Keyword(s):  
West Africa ◽  
Dna Sequence ◽  
Vigna Unguiculata ◽  
Sequence Data ◽  
Morphological Characteristics ◽  
Molecular Sequence Data ◽  
Dna Sequence Data ◽  
Wide Range ◽  
Cercosporoid Fungi ◽  
First Time

Cercosporoid fungi (Mycosphaerellaceae, Mycosphaerellales, Ascomycota) are one of the largest and most diverse groups of hyphomycetes causing a wide range of diseases of economically important plants as well as of plants in the wild. Although more than 6000 species are known for this group, the documentation of this fungal group is far from complete. Especially in the tropics, the diversity of cercosporoid fungi is poorly known. The present study aims to identify and characterise cercosporoid fungi collected on host plants belonging to Fabaceae in Benin, West Africa. Information on their morphology, host species and DNA sequence data (18S rDNA, 28S rDNA, ITS and tef1) is provided. DNA sequence data were obtained by a simple and non-culture-based method for DNA isolation which has been applied for cercosporoid fungi for the first time in the context of the present study. Among the loci used for the phylogenetic analysis, tef1 provided the best resolution together with the multigene dataset. Species delimitation in many cases, however, was only possible by combining molecular sequence data with morphological characteristics. Based on forty specimens recently collected in Benin, 18 species are presented with morphological descriptions, illustrations and sequence data. Among these, six species in the genus Cercospora and two species in Pseudocercospora are proposed as species new to science. The newly described species are Cercospora (C.) beninensis on Crotalaria macrocalyx, C. parakouensis on Desmodium tortuosum, C. rhynchophora on Vigna unguiculata, C. vignae-subterraneae on Vigna subterranea, C. tentaculifera on Vigna unguiculata, C. zorniicola on Zornia glochidiata, Pseudocercospora sennicola on Senna occidentalis and Pseudocercospora tabei on Vigna unguiculata. Eight species of cercosporoid fungi are reported for Benin for the first time, three of them, namely C. cf. canscorina, C. cf. fagopyri and C. phaseoli-lunati are new for West Africa. The presence of two species of cercosporoid fungi on Fabaceae previously reported from Benin, namely Nothopassalora personata and Passalora arachidicola, is confirmed.

scholarly journals Ecology and seasonality of Pseudo-nitzschia species (Bacillariophyceae) in the northwestern Adriatic Sea over a 30-years period (1988–2020)

2021 ◽  
Vol 22 (3) ◽  
pp. 505
Author(s):  
SONIA GIULIETTI ◽  
TIZIANA ROMAGNOLI ◽  
ALESSANDRA CAMPANELLI ◽  
CECILIA TOTTI ◽  
STEFANO ACCORONI
Keyword(s):  
Dna Sequence ◽  
Dna Sequences ◽  
Species Complex ◽  
Adriatic Sea ◽  
Sequence Data ◽  
Coastal Station ◽  
Dna Sequence Data ◽  
The Mean ◽  
First Time ◽  
North Western

The ecology and seasonality of Pseudo-nitzschia species and their contribution to phytoplankton community were analysed for the first time at the coastal station of the LTER-Senigallia-Susak transect (north-western Adriatic Sea) from 1988 to 2020. Species composition was addressed using DNA sequence data obtained from 106 monoclonal strains isolated from January 2018 to January 2020. The mean annual cycle of total phytoplankton in the study period (Feb 1988–Jan 2020) showed maximum abundances in winter followed by other peaks in spring and autumn. Diatoms were the main contributors in terms of abundance during the winter and the spring blooms. The autumn peak was due to phytoflagellates and diatoms. In summer phytoflagellates dominated the community, followed by diatoms and dinoflagellates, which in this season reached their annual maximum. Pseudo-nitzschia spp. represented on average 0.4–17.6% of diatom community, but during their blooms they could reach up to up to 90% of the total diatom abundances with 106 cells l-1. By LM, six different taxa were recognized: Pseudo-nitzschia cf. delicatissima and P. cf. pseudodelicatissima were the most abundant, followed by P. cf. fraudulenta, P. pungens, P. multistriata and P. cf. galaxiae. P. cf. fraudulenta and P. pungens were indicator taxa of winter. P. cf. delicatissima and P. cf. pseudodelicatissima were spring and summer taxa, respectively. P. galaxiae showed maximum abundances in autumn. DNA sequences revealed the presence of two species belonging to the ’P. seriata group’ (i.e. P. fraudulenta and P. pungens) and four species belonging to the ‘P. delicatissima group’ (P. calliantha and P. mannii within the P. pseudodelicatissima species complex, and P. delicatissima and P. cf. arenysensis within the P. delicatissima species complex). The presence of several cryptic and pseudo-cryptic species highlights the need to combine LM observations with DNA sequence data when the ecology of Pseudo-nitzschia is investigated. 

Phylogeny and revision of the Oriental leafhopper genus Amritodus (Hemiptera: Cicadellidae: Idiocerini)

2019 ◽  
Vol 189 (4) ◽  
pp. 1438-1463
Author(s):  
Qingquan Xue ◽  
Yalin Zhang
Keyword(s):  
New Species ◽  
Dna Sequence ◽  
Type Species ◽  
New Genus ◽  
Sequence Data ◽  
Morphological Characters ◽  
Mitochondrial Genes ◽  
Dna Sequence Data ◽  
Three New Species ◽  
First Time

Abstract The phylogeny of the Oriental leafhopper genus Amritodus is reconstructed, for the first time, based on 47 discrete morphological characters and DNA sequence data from one nuclear and two mitochondrial genes. The phylogenetic results show that Amritodus is not monophyletic, and its concept is narrowed here to include four species: Amritodus atkinsoni, Amritodus brevis, Amritodus brevistylus and Amritodus saeedi. The phylogenetic results support establishment of a new genus, Paramritodus gen. nov., with three new species,Paramritodus triangulus sp. nov. (type species), Paramritodus introflexus sp. nov., Paramritodus spatiosus sp. nov. and three species previously included in Amritodus: Paramritodus pistacious comb. nov., Paramritodus flavocapitatus comb. nov. and Paramritodus podocarpus comb. nov. In addition, Amritodus flavoscutatus is transferred from Amritodus to Hyalinocerus as Hyalinocerus flavoscutatus comb. nov. Keys to species of Amritodus and Paramritodus are provided.

Three new species and DNA sequence data of the rare South American water beetle genus Adelphydraena Perkins, 1989 (Coleoptera: Hydraenidae)

Zootaxa ◽  
2020 ◽  
Vol 4858 (1) ◽  
pp. 35-52
Author(s):  
PHILIP D. PERKINS ◽  
IGNACIO RIBERA
Keyword(s):  
New Species ◽  
High Resolution ◽  
Dna Sequence ◽  
Nuclear Dna ◽  
Sequence Data ◽  
South American ◽  
Dna Sequence Data ◽  
Water Beetle ◽  
Three New Species ◽  
First Time

Three new species of the very rarely collected water beetle genus Adelphydraena Perkins, 1989 are described from Brazil, Guyana, and Suriname. Illustrations of the male genitalia of the two previously known species in the genus, A. orchymonti Perkins and A. spangleri Perkins from Venezuela, are presented for the first time. High resolution digital images of the habitus of all species are given, and geographical distributions mapped. New species described are Adelphydraena amazonica n. sp. (Brazil), A. spinosa n. sp. (Guyana), and A. surinamensis n. sp. (Suriname). Mitochondrial and nuclear DNA sequence data is given for three of the five species currently known (A. orchymonti, A. spangleri, and A. amazonica). The monophyly of the genus Adelphydraena was strongly supported, but relationships between the sequenced species remain uncertain. 

Hyphomycetes from aquatic habitats in Southern China: Species of Curvularia (Pleosporaceae) and Phragmocephala (Melannomataceae)

Phytotaxa ◽  
2015 ◽  
Vol 226 (3) ◽  
pp. 201 ◽  
Author(s):  
HONG -YAN SU ◽  
Dhanushka Udayanga ◽  
Zong-long Luo ◽  
Dimuthu Manamgoda ◽  
Yong-Chang Zhao ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Sequence Data ◽  
Southern China ◽  
Freshwater Ecosystems ◽  
Aquatic Hyphomycetes ◽  
Dna Sequence Data ◽  
Freshwater Streams ◽  
The Family ◽  
Freshwater Habitats ◽  
First Time

Aquatic hyphomycetes are a diverse, polyphyletic group of asexually reproducing fungi involved in the decomposition of litter in freshwater ecosystems. Curvularia eragrostidis, C. verruculosa and Phragmocephala atra were identified from submerged wood collected from freshwater streams in Yunnan Province, Southwestern China. They were characterised based on morphology and LSU, ITS and SSU sequence data. Phylogenetic analysis of LSU sequences placed the isolates within the order Pleosporales. Curvularia eragrostidis and C. verruculosa are reported from freshwater habitats for the first time. An epitype is designated for Curvularia verruculosa. This is the first phylogenetic placement of the genus Phragmocephala in the family Melanommataceae in Dothideomycetes, providing new DNA sequence data. A new species, Phragmocephala garethjonesii is introduced based on DNA sequence data and morphology. Descriptions and illustrations are provided for the species with notes on their taxonomy and phylogeny.

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