Unravelling unexplored diversity of cercosporoid fungi (Mycosphaerellaceae, Mycosphaerellales, Ascomycota) in tropical Africa

Cercosporoid fungi (Mycosphaerellaceae, Mycosphaerellales, Ascomycota) are one of the largest and most diverse groups of hyphomycetes causing a wide range of diseases of economically important plants as well as of plants in the wild. Although more than 6000 species are known for this group, the documentation of this fungal group is far from complete. Especially in the tropics, the diversity of cercosporoid fungi is poorly known. The present study aims to identify and characterise cercosporoid fungi collected on host plants belonging to Fabaceae in Benin, West Africa. Information on their morphology, host species and DNA sequence data (18S rDNA, 28S rDNA, ITS and tef1) is provided. DNA sequence data were obtained by a simple and non-culture-based method for DNA isolation which has been applied for cercosporoid fungi for the first time in the context of the present study. Among the loci used for the phylogenetic analysis, tef1 provided the best resolution together with the multigene dataset. Species delimitation in many cases, however, was only possible by combining molecular sequence data with morphological characteristics. Based on forty specimens recently collected in Benin, 18 species are presented with morphological descriptions, illustrations and sequence data. Among these, six species in the genus Cercospora and two species in Pseudocercospora are proposed as species new to science. The newly described species are Cercospora (C.) beninensis on Crotalaria macrocalyx, C. parakouensis on Desmodium tortuosum, C. rhynchophora on Vigna unguiculata, C. vignae-subterraneae on Vigna subterranea, C. tentaculifera on Vigna unguiculata, C. zorniicola on Zornia glochidiata, Pseudocercospora sennicola on Senna occidentalis and Pseudocercospora tabei on Vigna unguiculata. Eight species of cercosporoid fungi are reported for Benin for the first time, three of them, namely C. cf. canscorina, C. cf. fagopyri and C. phaseoli-lunati are new for West Africa. The presence of two species of cercosporoid fungi on Fabaceae previously reported from Benin, namely Nothopassalora personata and Passalora arachidicola, is confirmed.

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Sequences of the second internal transcribed spacer of ribosomal DNA for three species of Trichostrongylus (Nematoda: Trichostrongylidae) from sheep in Russia

Helminthologia ◽

10.2478/s11687-007-0002-z ◽

2007 ◽

Vol 44 (2) ◽

pp. 43-46 ◽

Cited By ~ 5

Author(s):

D. Kuznetsov ◽

N. Kuznetsova

Keyword(s):

Dna Sequence ◽

Intraspecific Variation ◽

Ribosomal Dna ◽

Internal Transcribed Spacer ◽

Sequence Data ◽

Moscow Region ◽

Dna Sequence Data ◽

Interspecific Differences ◽

Second Internal Transcribed Spacer ◽

First Time

AbstractFor the first time, DNA sequence data were obtained for three species of Trichostrongylus from Russia. Internal transcribed spacer (ITS-2) of ribosomal DNA was sequenced for T. axei, T. colubriformis and T. probolurus from sheep from the Moscow region. ITS-2 rDNA length was estimated as 238 nucleotides for T. colubriformis and T. probolurus and 237 nucleotides for T. axei. The G+C content of the ITS-2 sequences of T. colubriformis, T. axei and T. probolurus were 31 %, 32 % and 34 % respectively. The level of interspecific differences in ITS-2 of rDNA of T. axei, T. probolurus and T. colubriformis ranged from 3 to 4 %. The ITS-2 sequences from the Russian specimens were compared with those of T. axei, T. probolurus and T. colubriformis from Australia and Germany. Intraspecific variation ranged from 0 % in T. colubriformis to 3.0 % in T. axei.

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Immature stages, host plants and natural enemies of Henosepilachna implicata (Mulsant) (Coleoptera: Coccinellidae) with DNA sequence data and a new synonym and notes on some Indian species of Epilachnini

Zootaxa ◽

10.11646/zootaxa.4970.3.5 ◽

2021 ◽

Vol 4970 (3) ◽

pp. 533-546

Author(s):

J. POORANI ◽

R. G. BOOTH ◽

R. GANDHI GRACY ◽

C. ANURADHA ◽

R. THANIGAIRAJ ◽

...

Keyword(s):

Dna Sequence ◽

Natural Enemies ◽

Host Plants ◽

Sequence Data ◽

Indian Species ◽

Life Stages ◽

Important Species ◽

Dna Sequence Data ◽

New Synonym ◽

First Time

Life stages of Henosepilachna implicata (Mulsant), an economically important species of Epilachnini in India, are documented and illustrated. Mitochondrial DNA sequence data is provided for the first time for H. implicata with additional details on its host plants, distribution, and natural enemies. Its similarities and differences with other common pestiferous Henosepilachna spp. in India such as H. vigintioctopunctata (F.), H. septima (Dieke) and H. pusillanima (Mulsant) are discussed. Epilachna circularis Korschefsky, 1933 is found to be conspecific with H. implicata and is reduced to a junior synonym of the latter (new synonym). Notes are given on the distribution and natural enemies of some other species of Epilachnini of the Indian region.

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The Genera of Fungi – G6: Arthrographis, Kramasamuha, Melnikomyces, Thysanorea, and Verruconis

Fungal Systematics and Evolution ◽

10.3114/fuse.2020.06.01 ◽

2020 ◽

Vol 6 (1) ◽

pp. 1-24

Author(s):

M. Hernández-Restrepo ◽

A. Giraldo ◽

R. van Doorn ◽

M.J. Wingfield ◽

J.Z. Groenewald ◽

...

Keyword(s):

New Species ◽

Dna Sequence ◽

Type Species ◽

New Genus ◽

Sequence Data ◽

Dna Sequence Data ◽

Two New Species ◽

First Time

The Genera of Fungi series, of which this is the sixth contribution, links type species of fungal genera to their morphology and DNA sequence data. Five genera of microfungi are treated in this study, with new species introduced in Arthrographis, Melnikomyces, and Verruconis. The genus Thysanorea is emended and two new species and nine combinations are proposed. Kramasamuha sibika, the type species of the genus, is provided with DNA sequence data for first time and shown to be a member of Helminthosphaeriaceae (Sordariomycetes). Aureoconidiella is introduced as a new genus representing a new lineage in the Dothideomycetes.

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Revision of Batrachospermum sections Acarposporophytum and Aristata (Batrachospermales, Rhodophyta) with the establishment of the new genera Acarposporophycos and Visia

Phytotaxa ◽

10.11646/phytotaxa.395.2.1 ◽

2019 ◽

Vol 395 (2) ◽

pp. 51 ◽

Cited By ~ 6

Author(s):

ORLANDO NECCHI JR ◽

AURO GARCIA FILHO ◽

MONICA O. PAIANO

Keyword(s):

Dna Sequence ◽

Sequence Data ◽

Morphological Characteristics ◽

Sequence Divergence ◽

Morphological Evidence ◽

Similar Species ◽

Type Specimens ◽

Generic Level ◽

Dna Sequence Data ◽

Cell Layers

Sections of the paraphyletic genus Batrachospermum have been recently investigated using molecular (DNA sequence data) and morphological evidence. Some sections have been recognized as distinct genera in order to resolve paraphyly. Batrachospermum species of sections Acarposporophytum and Aristata are showed to form well-supported clades and, in this study, we propose to raise them to the generic level, Acarposporophycos gen. nov. and Visia gen. nov., respectively. In addition, we re-evaluated the characters used to circumscribe species by reexamining type specimens as well as new collections. Acarposporophycos, with the sole species A. brasiliensis, is characterized by the lack of carposporophytic stage, with the fertilized carpogonia germinating to form directly the filaments of the ‘Chantransia’ stage on the gametophyte. Of the five previously accepted species in the genus Visia, we recognize four species: V. cayennensis—type species (synonym Batrachospermum beraense), V. cylindrocellularis, V. longiarticulata and V. turgida. The species are circumscribed on the basis of morphological characteristics (shape and cell layers of primary fascicles, occurrence of secondary fascicles, post-fertilization development of carpogonia) and DNA sequence data (rbcL and COI-5P). Specimens described as Visia cayennensis from Australia and Brazil had a high sequence divergence in comparison to those from or near the type locality (French Guiana), and are here referred to as Visia spp. Further studies are recommended to better discriminate these morphologically similar species on a broad geographical scale and define their status at the species level. Descriptions, identification key and photomicrographs are presented for each recognized species.

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Proteaceae: Diverse approaches toward new syntheses

Australian Systematic Botany ◽

10.1071/sb97025 ◽

1998 ◽

Vol 11 (4) ◽

pp. 631 ◽

Cited By ~ 3

Author(s):

Barbara G. Briggs

Keyword(s):

Dna Sequence ◽

Reproductive Biology ◽

Fossil Record ◽

Sequence Data ◽

Large Body ◽

Dna Sequence Data ◽

Wide Range ◽

Phylogenetic Hypotheses

The 1996 Commemorative Proteaceae Conference drew attention to the large body of work proceeding on all southern continents, the extensive and informative fossil record, and the wide range of studies in ecology and reproductive biology. DNA sequence data and organogeny are producing major insights at the upper taxonomic levels, new phylogenetic hypotheses and classifications are emerging in respect of the recognition of subfamilies and the relationships and composition of tribes, and there is also evidence from morphology and DNA sequence data that several genera are paraphyletic.

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Toward a Natural Classification of Botryosphaeriaceae: A Study of the Type Specimens of Botryosphaeria sensu lato

Frontiers in Microbiology ◽

10.3389/fmicb.2021.737541 ◽

2021 ◽

Vol 12 ◽

Author(s):

Ying Zhang ◽

Yupei Zhou ◽

Wei Sun ◽

Lili Zhao ◽

D. Pavlic-Zupanc ◽

...

Keyword(s):

Dna Sequence ◽

Dna Sequences ◽

Sequence Data ◽

Morphological Characteristics ◽

Taxonomic Status ◽

Morphological Characters ◽

Phylogenetic Position ◽

Type Specimens ◽

Natural Classification ◽

Dna Sequence Data

The genus Botryosphaeria includes more than 200 epithets, but only the type species, Botryosphaeria dothidea and a dozen or more other species have been identified based on DNA sequence data. The taxonomic status of the other species remains unconfirmed because they lack either morphological information or DNA sequence data. In this study, types or authentic specimens of 16 “Botryosphaeria” species are reassessed to clarify their identity and phylogenetic position. nuDNA sequences of four regions, ITS, LSU, tef1-α and tub2, are analyzed and considered in combination with morphological characteristics. Based on the multigene phylogeny and morphological characters, Botryosphaeria cruenta and Botryosphaeria hamamelidis are transferred to Neofusicoccum. The generic status of Botryosphaeria aterrima and Botryosphaeria mirabile is confirmed in Botryosphaeria. Botryosphaeria berengeriana var. weigeliae and B. berengeriana var. acerina are treated synonyms of B. dothidea. Botryosphaeria mucosa is transferred to Neodeightonia as Neodeightonia mucosa, and Botryosphaeria ferruginea to Nothophoma as Nothophoma ferruginea. Botryosphaeria foliicola is reduced to synonymy with Phyllachorella micheliae. Botryosphaeria abuensis, Botryosphaeria aesculi, Botryosphaeria dasylirii, and Botryosphaeria wisteriae are tentatively kept in Botryosphaeria sensu stricto until further phylogenetic analysis is carried out on verified specimens. The ordinal status of Botryosphaeria apocyni, Botryosphaeria gaubae, and Botryosphaeria smilacinina cannot be determined, and tentatively accommodate these species in Dothideomycetes incertae sedis. The study demonstrates the significance of a polyphasic approach in characterizing type specimens, including the importance of using of DNA sequence data.

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Delayed germination of basidiospores in temperate species of Crepidotus (Fr.) Staude

Canadian Journal of Botany ◽

10.1139/b02-009 ◽

2002 ◽

Vol 80 (3) ◽

pp. 280-287 ◽

Cited By ~ 5

Author(s):

M C Aime ◽

O K Miller Jr.

Keyword(s):

Dna Sequence ◽

Sequence Data ◽

Abiotic Factors ◽

Large Subunit ◽

Early Spring ◽

Single Spore ◽

Dna Sequence Data ◽

Delayed Germination ◽

First Time ◽

Ribosomal Dna Sequence

Delayed basidiospore germination (endogenous dormancy) for a number of species of Crepidotus (Agaricales: Crepidotaceae) is reported for the first time. Ninety percent of recovered single spore isolates germinated between 18 and 36 weeks after collection; average germination was 25 weeks after collection. The period in which 90% of germinations occurred was between 17 February and 19 April, with 50% of recovered isolates germinating in March. Many abiotic factors were experimentally manipulated in an effort to reduce or alter the necessary incubation period without effect. The latent period was consistent for a given collection, with the majority of recovered isolates from fall-fruiting collections germinating during early spring, regardless of whether spores were plated immediately after harvesting or stored for one to several months prior to plating. The identity of the cultures derived from delayed germination was confirmed by DNA sequencing.Key words: basidiospore, dormancy, single spore isolates, Crepidotaceae, large subunit ribosomal DNA sequence data, phylogeny.

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Ecology and seasonality of Pseudo-nitzschia species (Bacillariophyceae) in the northwestern Adriatic Sea over a 30-years period (1988–2020)

Mediterranean Marine Science ◽

10.12681/mms.26021 ◽

2021 ◽

Vol 22 (3) ◽

pp. 505

Author(s):

SONIA GIULIETTI ◽

TIZIANA ROMAGNOLI ◽

ALESSANDRA CAMPANELLI ◽

CECILIA TOTTI ◽

STEFANO ACCORONI

Keyword(s):

Dna Sequence ◽

Dna Sequences ◽

Species Complex ◽

Adriatic Sea ◽

Sequence Data ◽

Coastal Station ◽

Dna Sequence Data ◽

The Mean ◽

First Time ◽

North Western

The ecology and seasonality of Pseudo-nitzschia species and their contribution to phytoplankton community were analysed for the first time at the coastal station of the LTER-Senigallia-Susak transect (north-western Adriatic Sea) from 1988 to 2020. Species composition was addressed using DNA sequence data obtained from 106 monoclonal strains isolated from January 2018 to January 2020. The mean annual cycle of total phytoplankton in the study period (Feb 1988–Jan 2020) showed maximum abundances in winter followed by other peaks in spring and autumn. Diatoms were the main contributors in terms of abundance during the winter and the spring blooms. The autumn peak was due to phytoflagellates and diatoms. In summer phytoflagellates dominated the community, followed by diatoms and dinoflagellates, which in this season reached their annual maximum. Pseudo-nitzschia spp. represented on average 0.4–17.6% of diatom community, but during their blooms they could reach up to up to 90% of the total diatom abundances with 106 cells l-1. By LM, six different taxa were recognized: Pseudo-nitzschia cf. delicatissima and P. cf. pseudodelicatissima were the most abundant, followed by P. cf. fraudulenta, P. pungens, P. multistriata and P. cf. galaxiae. P. cf. fraudulenta and P. pungens were indicator taxa of winter. P. cf. delicatissima and P. cf. pseudodelicatissima were spring and summer taxa, respectively. P. galaxiae showed maximum abundances in autumn. DNA sequences revealed the presence of two species belonging to the ’P. seriata group’ (i.e. P. fraudulenta and P. pungens) and four species belonging to the ‘P. delicatissima group’ (P. calliantha and P. mannii within the P. pseudodelicatissima species complex, and P. delicatissima and P. cf. arenysensis within the P. delicatissima species complex). The presence of several cryptic and pseudo-cryptic species highlights the need to combine LM observations with DNA sequence data when the ecology of Pseudo-nitzschia is investigated.

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Identification of endophytic fungi from leaves of Pandanaceae based on their morphotypes and DNA sequence data from southern Thailand

MycoKeys ◽

10.3897/mycokeys.32.23670 ◽

2018 ◽

Vol 33 ◽

pp. 25-67 ◽

Cited By ~ 2

Author(s):

Saowaluck Tibpromma ◽

Kevin D. Hyde ◽

Jayarama D. Bhat ◽

Peter E. Mortimer ◽

Jianchu Xu ◽

...

Keyword(s):

Dna Sequence ◽

Endophytic Fungi ◽

Sequence Data ◽

Phylogenetic Analyses ◽

Morphological Characteristics ◽

Taxonomic Status ◽

Universal Primers ◽

Specific Gene ◽

Dna Sequence Data ◽

Southern Thailand

The authors established the taxonomic status of endophytic fungi associated with leaves of Pandanaceae collected from southern Thailand. Morphotypes were initially identified based on their characteristics in culture and species level identification was done based on both morphological characteristics and phylogenetic analyses of DNA sequence data. Twenty-two isolates from healthy leaves were categorised into eight morphotypes. Appropriate universal primers were used to amplify specific gene regions and phylogenetic analyses were performed to identify these endophytes and established relationships with extant fungi. The authors identified both ascomycete and basidiomycete species, including one new genus, seven new species and nine known species. Morphological descriptions, colour plates and phylogenies are given for each taxon.

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Reappraisal of Immotthia in Dictyosporiaceae, Pleosporales: Introducing Immotthia bambusae sp. nov. and Pseudocyclothyriella clematidis comb. et gen. nov. Based on Morphology and Phylogeny

Frontiers in Microbiology ◽

10.3389/fmicb.2021.656235 ◽

2021 ◽

Vol 12 ◽

Author(s):

Hong-Bo Jiang ◽

Rajesh Jeewon ◽

Samantha C. Karunarathna ◽

Chayanard Phukhamsakda ◽

Mingkwan Doilom ◽

...

Keyword(s):

Dna Sequence ◽

Phylogenetic Trees ◽

Sequence Data ◽

Phylogenetic Analyses ◽

Morphological Characteristics ◽

Single Species ◽

Type Specimen ◽

Morphological Identification ◽

Ongoing Research ◽

Dna Sequence Data

Immotthia is a poorly known genus, and currently, no DNA sequence data are available to ascertain its proper phylogenetic placement and evolutionary relationships with other bitunicate fungi. To date, there are only two species accepted in the genus. During our ongoing research study of bambusicolous fungi in southwest China and Thailand, a fungus associated with stromata of Hypoxylon sp. was found on dead bamboo culms in Loei Province, Thailand. Preliminary morphological identification revealed that the fungal collection belongs to Immotthia. A novel species, Immotthia bambusae, is introduced herein based on a comparison of morphological characteristics with the type specimen of I. hypoxylon (≡ Amphisphaeria hypoxylon Ellis and Everh.), a synonym of I. atrograna (Cooke and Ellis) M. E. Barr. Phylogenetic analyses of a concatenated ITS, LSU, SSU, and TEF1-α DNA sequence matrix showed that Immotthia belongs to Dictyosporiaceae, Pleosporales. Despite I. bambusae strains constituting a supported subclade, they are nested with the genus Pseudocoleophoma. Pseudocoleophoma clematidis is morphologically different from all other Pseudocoleophoma species, while its conidial characteristics are similar to Cyclothyriella. Multigene phylogenetic analyses showed that P. clematidis formed a clade basal to Immotthia, separated from Pseudocoleophoma with strong statistical support. Therefore, we introduce a monotypic genus, Pseudocyclothyriella Phukhams. and Phookamsak, gen. nov. to accommodate the single species, Pseudocyclothyriella clematidis (Phukhams. and K. D. Hyde) Phukhams. and Phookamsak, comb. nov. Detailed descriptions, color micrographs, and phylogenetic trees to show the placement of the new taxa are provided. In addition, an updated taxonomic treatment of the genera Immotthia and Pseudocyclothyriella is also provided based on the study of the type materials and phylogeny generated from DNA sequence data.

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