New records of Pluteus section Hispidoderma in Turkey Based on Morphological Characteristics and Molecular Data

Phytotaxa ◽  
2019 ◽  
Vol 413 (3) ◽  
pp. 175-206 ◽  
Author(s):  
OGUZHAN KAYGUSUZ ◽  
IBRAHIM TÜRKEKUL ◽  
HENNING KNUDSEN ◽  
ÖMER FARUK ÇOLAK

Twenty-six species from various sections of the genus Pluteus have been recorded from Turkey until now. In this study, phylogenetic analyses inferred from the nuclear ribosomal internal transcribed spacer (nrITS) were conducted for species delimitation and for comparisons with closely related taxa of Pluteus sect. Hispidoderma in Turkey. Our results show that P. granularis is recorded in Turkey and Europe for the first time, P. granulatus in Turkey for the first time and in Europe for the third time, P. umbrosoides in Turkey for the first time and in the world for the second time, and P. variabilicolor in Turkey for the first time. Also in this study, sequencing data for P. leoninus, P. plautus, P. roseipes, P. semibulbosus and P. umbrosus as well as the above-mentioned new records are presented for the first time from Turkey as a different geographical area. Analyses of the ITS showed that the lowest sequence divergence among in-group taxa was observed between P. semibulbosus and P. aff. semibulbosus 0.010% (SE = 0.004), the next was between P. chrysaegis and P. conizatus var. africanus 0.012% (SE = 0.004). The highest sequence divergence with in-group taxa was found between P. plautus and P. umbrosoides, 0.351% (SE = 0.039). In terms of habitat, Fagus orientalis is recorded as a new host for P. granulatus, P. granularis, P. umbrosoides, and P. umbrosus; Picea orentalis is a new host to P. granularis; Carpinus betulus for P. umbrosus; and the relict endemic Liquidambar orientalis for P. variabilicolor. Finally, this study provides detailed morphological descriptions, macro photographs and microstructure drawings relating to species of Pluteus sect. Hispidoderma from Turkey, which are rare, interesting and noteworthy. An identification key is provided for present species of Pluteus sect. Hispidoderma occurring in the country.

2015 ◽  
Vol 29 (5) ◽  
pp. 473 ◽  
Author(s):  
Takafumi Nakano ◽  
Son Truong Nguyen

The family Salifidae is a predaceous leech taxon in the suborder Erpobdelliformes. Although Salifidae is widely distributed in the African, Oriental, Indo-Malayan, Sino-Japanese and Australasian regions, the phylogenetic relationships of the family Salifidae have never been tested using molecular data obtained from leeches collected from the family distributional range. A salifid species was collected for the first time in Vietnam, and relevant morphological and molecular data are presented here. Because the Vietnamese salifid species possesses unique morphological characteristics among the known salifid species, this species is herein described as a new species, Salifa motokawai, sp. nov. Phylogenetic analyses based on nuclear 18S rRNA and histone H3, as well as mitochondrial cytochrome c oxidase subunit I, tRNACys, tRNAMet, 12S rRNA, tRNAVal, 16S rRNA, tRNALeu and NADH dehydrogenase subunit 1 markers demonstrate that the Vietnamese salifid species is a close congener with the African Salifa perspicax and the Malagasy Linta be. Furthermore, molecular data revealed non-monophyly of the Asian salifid leeches. According to the observed phylogenetic relationships and morphological characteristics of the Vietnamese Salifa motokawai, sp. nov., the current classification of salifid taxa should be revised.


Phytotaxa ◽  
2016 ◽  
Vol 286 (4) ◽  
pp. 211 ◽  
Author(s):  
BENJARONG THONGBAI ◽  
RODHAM E. TULLOSS ◽  
STEVEN L. MILLER ◽  
KEVIN D. HYDE ◽  
JIE CHEN ◽  
...  

Mushrooms belonging to the genus Amanita were collected during a fungal biodiversity study in northern Thailand in 2012–2014. Morphological characteristics and molecular phylogenetic analyses were used to identify the mushrooms to species. Amanita castanea is described as new to science and compared with phenetically and phylogenetically similar species. It is assignable to Amanita stirps Citrina within Amanita series Mappae. Four other species, A. concentrica, A. rimosa, A. cf. rubromarginata and A. zangii are first reports for Thailand; detailed morphological and molecular data are provided for the Thai material.


Phytotaxa ◽  
2015 ◽  
Vol 239 (1) ◽  
pp. 17
Author(s):  
Larissa Bernardino Moro ◽  
Gregorio Delgado ◽  
Iracema Helena SCHOENLEIN-CRUSIUS

Clathrosporium retortum sp. nov., collected on submerged mixed leaf litter samples at Ilha do Cardoso State Park, São Paulo state, Brazil, is described based on morphological and molecular data. The fungus is characterized by forming whitish, dense, subglobose to irregular propagules, hyaline to subhyaline when young, subhyaline to dark brown at maturity, that are formed by densely interwoven conidial filaments with each conidial cell repeatedly branching bilaterally or occasionally unilaterally. Phylogenetic analyses using partial LSU nrDNA sequence data suggest that C. retortum belongs in the Sordariomycetes (Ascomycota) where it forms a well-supported clade with Clohesia corticola in the Sordariomycetidae, but its ordinal or familial placement remains unresolved. Its phylogenetic placement confirms the polyphyletic nature of aeroaquatic fungi like Clathrosporium, as it was distantly related to one available sequence in GenBank named as C. intricatum, the type species, which is phylogenetically related to the Helotiales (Leotiomycetes). However, due to lack of authenticity of the identity of this sequence with the type specimen of C. intricatum, a broad concept of Clathrosporium is tentatively adopted here to accommodate the present fungus instead of introducing a new genus. Beverwykella clathrata, Helicoön septatissimum and Peyronelina glomerulata are recorded for the first time from Brazil. Cancellidium applanatum and Candelabrum brocchiatum are new records for the state of São Paulo.


2018 ◽  
Vol 67 (1) ◽  
pp. 7-57 ◽  
Author(s):  
Miloš Černý ◽  
Rui Andrade ◽  
Ana Rita Gonçalves ◽  
Michael von Tschirnhaus

Abstract New records of 110 species of the acalyptrate Diptera family Agromyzidae are given from Portugal, including Madeira, Porto Santo and the Azores. A quarantine plant pest, Nemorimyza maculosa (Malloch, 1913), was detected in the Old World for the first time. Details on Phytobia xylem- miners and a parthenogenetic Phytomyza species are recorded together with new distribution data. For certain species morphological and taxonomic notes and discussions on known or new host plants are added. A complete checklist of Agromyzidae of Portugal is presented.


2017 ◽  
Vol 18 ◽  
pp. 56 ◽  
Author(s):  
Ioannis Th. Anagnostopoulos

From the study of the Greek bumblebee fauna (Hymenoptera: Apidae, Bombini), species lists have been published based on both literature records and original data from collected bees. Since 1995 a special effort to confirm with newly collected bees all bumblebee species reported in literature records for Greece has been in progress. Although numerous specimens have been collected and examined and in some instances yielding new Bombus species for the Greek insect fauna, some species, mainly those reported in older references, have not yet been found. Recently, identification of bumblebees collected in the Florina Prefecture - Northwest Macedonia, during the years 2006 and 2007 yielded information for two “literature cited” species, Bombus subterraneus (Linnaeus 1758) and Bombus cryptarum (Fabricius 1775). A B. subterraneus queen (collected at 40°47´38N, 21°26´10E on Vicia cracca) was distinguished by morphological characteristics and a worker B. cryptarum (collected at 40°41´58,7N, 21°28´18,5E on Echium spp) was revealed using mitochondrial DNA RFLP analysis of the CO1 gene. These new records from Florina are provided with comments, confirming the species presence in Greece for the first time after approximately 40 years.


2010 ◽  
Vol 47 (2) ◽  
pp. 105-114 ◽  
Author(s):  
B. Koubková ◽  
V. Baruš ◽  
I. Hodová

AbstractThree nematode species of Cithariniella (Pharyngodonidae), C. citharini, C. khalili, and C. gonzalesi, were recorded from the recta of squeaker (Mochokidae: Siluriformes) and citharinid (Citharinidae: Characiformes) fishes from Senegal, West Africa. Morphological characteristics obtained by scanning electron microscopy (form of oral aperture and cephalic papillae, presence of lateral alae, distribution and form of cloacal papillae, simple or paired papillae on tail of males, eggs with numerous long filaments on each pole in females) correspond well to the generic diagnosis and represent species differences. The shape and size of the cephalic papillae and lips were identified as a new determination feature. C. gonzalesi is reported for the first time from Senegal and its host, Paradistichodus dimidiatus, represents a new host record. A tentative simple key for differentiating C. citharini, C. khalili, C. gonzalesi and C. petterae is provided based upon these results.


Parasitology ◽  
2008 ◽  
Vol 136 (2) ◽  
pp. 241-252 ◽  
Author(s):  
T. L. F. LEUNG ◽  
D. B. KEENEY ◽  
R. POULIN

SUMMARYRecent studies have shown that some digenean trematodes previously identified as single species due to the lack of distinguishing morphological characteristics actually consist of a number of genetically distinct cryptic species. We obtained mitochondrial 16S and nuclear ITS1 sequences for the redial stages of Acanthoparyphium sp. and Curtuteria australis collected from snails and whelks at various locations around Otago Peninsula, New Zealand. These two echinostomes are well-known host manipulators whose impact extends to the entire intertidal community. Using phylogenetic analyses, we found that Acanthoparyphium sp. is actually composed of at least 4 genetically distinct species, and that a cryptic species of Curtuteria occurs in addition to C. australis. Molecular data obtained for metacercariae dissected from cockle second intermediate hosts matched sequences obtained for Acanthoparyphium sp. A and C. australis rediae, respectively, but no other species. The various cryptic species of both Acanthoparyphium and Curtuteria also showed an extremely localized pattern of distribution: some species were either absent or very rare in Otago Harbour, but reached far higher prevalence in nearby sheltered inlets. This small-scale spatial segregation is unexpected as shorebird definitive hosts can disperse trematode eggs across wide geographical areas, which should result in a homogeneous mixing of the species on small geographical scales. Possible explanations for this spatial segregation of the species include sampling artefacts, local adaptation by first intermediate hosts, environmental conditions, and site fidelity of the definitive hosts.


2016 ◽  
Vol 48 (5) ◽  
pp. 387-421 ◽  
Author(s):  
Daphne F. STONE ◽  
James W. HINDS ◽  
Frances L. ANDERSON ◽  
James C. LENDEMER

AbstractA revision of the North American members of the Leptogium saturninum group (i.e. species with long lower-surface hairs, isidia, and usually smooth upper surface) is presented based on molecular phylogenetic analyses of mtSSU and nrITS sequence data, together with an extensive morphological study. Three species supported by both molecular and morphological characteristics are recognized: L. acadiense sp. nov. (distinguished by granular saturninum-type isidia, medulla composed of irregularly arranged or perpendicular hyphae), L. cookii sp. nov. (distinguished by cylindrical saturninum-type isidia) and L. hirsutum (distinguished by hirsutum-type isidia and medulla composed of loosely intertwined hyphae). One species supported by morphological characteristics, but for which no molecular data could be generated, is also recognized: L. compactum sp. nov. (distinguished by hirsutum-type isidia and medulla composed of tightly packed hyphae). Finally, L. saturninum (distinguished by granular saturninum-type isidia and medulla composed of perpendicular and parallel hyphae) is supported by morphological characteristics but molecular data from geographically diverse populations, including those near the type locality, indicate that the morphologically defined species is paraphyletic. Leptogium burnetiae is excluded from North American based on morphological study of the type. The species are described and illustrated in detail, and are distinguished morphologically by their isidium development, morphology of mature isidia, and pattern of hyphae in the medulla in transverse sections near lobe margins. A key to the members of the L. saturninum group and related species is also presented.


Phytotaxa ◽  
2019 ◽  
Vol 404 (3) ◽  
pp. 91
Author(s):  
JIE CHEN ◽  
PHILIPPE CALLAC ◽  
RÉGULO CARLOS LLARENA-HERNÁNDEZ ◽  
GERARDO GERARDO MATA

Agaricus is a species-rich genus with more than 500 species over the world. Recent studies on tropical Agaricus revealed new tropical clades and a revised taxonomic system was consequently developed. Agaricus subg. Minoriopsis, a sixth subgenus was recently added, comprising species exclusively from the Americas. The diversity of Agaricus is little studied in Mexico, despite this country has a long history in the consumption of wild edible fungi. In this paper, we introduce a new species A. guzmanii and a new record for A. globocystidiatus from Mexico based on morphological characteristics and molecular data. Phylogenetic analyses indicate that they belong to A. subg. Minoriopsis.


Phytotaxa ◽  
2017 ◽  
Vol 292 (1) ◽  
pp. 19 ◽  
Author(s):  
ASANKA R. BANDARA ◽  
SAMANTHA C. KARUNARATHNA ◽  
ALAN J.L. PHILLIPS ◽  
PETER E. MORTIMER ◽  
JIANCHU XU ◽  
...  

The identification of Auricularia species has previously relied on the examination of macroscopic features of mushroom samples collected in Thailand. These features may vary with age, light exposure, moisture and other factors, making such identification prone to error. This study uses morphological characteristics and molecular data to improve upon previous classifications of Auricularia species. Fruiting bodies collected during recent field excursions and previous collections from herbaria were examined and sequenced for two loci, viz. ITS and rpb2. Auricularia asiatica is described as a new species based on both morphological characteristics and molecular data, and is introduced with a full description, illustrations and colour photographs. Auricularia cornea and A. villosula are described as new records from Thailand. A combined ITS and rpb2 phylogenetic tree is provided, showing the placement of five Auricularia species found in Thailand.


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