scholarly journals A Robust Protocol for Managing Microbial Contamination of <i>In-vitro</i> Banana Plants

2021 ◽  
Vol 6 (3) ◽  
pp. 95
Author(s):  
Mark Ochieng Adero ◽  
Easter David Syombua ◽  
Richard Oduor
Keyword(s):  
Microbial Contamination ◽  
Robust Protocol

P–218 Analysis of the occurrence of microbial contamination in IVF culture system and the effect of microorganisms on embryo development and clinical outcomes

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
F Du ◽  
R Li ◽  
Q Zhang ◽  
W Wang
Keyword(s):  
Escherichia Coli ◽  
In Vitro Culture ◽  
Clinical Outcomes ◽  
Embryo Transfer ◽  
Follicular Fluid ◽  
Culture System ◽  
Microbial Contamination ◽  
Culture Medium ◽  
Ivf Et

Abstract Study question what is the source, prevalence, and influence of microbial contamination on in vitro fertilization (IVF) and embryo transfer (ET) cycles? Summary answer Microbial contamination mainly occurs on Day 2, most caused by Escherichia coli carried with semen. ICSI could prevent contamination effectively and get good clinical outcomes. What is known already Microbial contamination occurs in IVF-ET system occasionally, which is hard to stop happening. The IVF culture system and laboratory environment, the patients’ follicular fluid and semen are not absolutely sterile, while the antibiotics in culture medium isn’t effective for all microbe types, and the artificial operations may bring in microbes. Generally, microbial contamination leads to degradation of embryos, reduction the number of embryos available, and infection of female reproductive tract, which would increase the cost of patients’ time, money, and bring psychological damages. A better understanding of embryo contamination in IVF culture system is of added value. Study design, size, duration A total of 29583 IVF-ET cycles were enrolled in this prospective observational study, from January 2010 to December 2020, included 70 microbial contamination cycles discovered in Day1-Day3 (D1-D3) of in vitro culture. Follicular fluid and semen saved on oocyte retrieval day, and culture medium contaminated were examined and identified for microorganisms at each contamination cycle. Participants/materials, setting, methods Compared the contamination rate of different insemination methods (IVF/ICSI/IVF+ICSI), different in vitro culture days (D1-D3), and different samples examination (follicular fluid, semen, culture medium) respectively, identified the source of microorganism types, compared the IVF culture outcomes and clinical outcomes between total contamination group (TC group, 42 cases) and partial contamination group (PC group, 28 cases). Main results and the role of chance A total of 70 microbial contamination cases occurred in 29583 oocyte retrieving cycles (0.24%), and it was observed only in IVF embryos but never in ICSI (Intracytoplasmic sperm injection) embryos. 38 contamination cases occurred on D2 with a highest ratio (54.3%) compared to D1 (32.9%) and D3(12.9%); Compared with follicular fluid, semen was the main cause inducing contamination from D1 to D3, and Escherichia coli in semen and culture medium, Enterococcus faecalis in follicular fluid proved to be the most common sources. Compared with TC group, the PC group showed a lower rate of No-available embryos (21.4% vs 81.0%) and a higher rate of blastocyst formation (41.2% vs 28.6%), In addition, the clinical pregnancy rate of PC group was higher than that of TC group in both fresh and frozen-thawed embryo transfer cycles (31.3% vs 16.7%, 38.5% vs 0.0%). Limitations, reasons for caution Further study is still necessary to better understand the sources that induce microbial contamination embryos, and more efficient methods are required to remove the microbes on these contaminated embryos so as better develop and manage a sterile micro-environment for successful embryo growth. Wider implications of the findings: The differential embryonic microbe types associated to different IVF culture and clinical outcomes in patients undergoing IVF-ET might have profound implications for understanding the microbial sources and making a better management of IVF culture system. Trial registration number Not applicable

Lactobacillus plantarum ZJ316 improves the quality of Stachys sieboldii Miq. pickle by inhibiting harmful bacteria growth and degrading nitrite, promoting the gut microbiota health in vitro

2022 ◽  
Author(s):  
Shu Ting Hang ◽  
Ling zhou Zeng ◽  
Jia run Han ◽  
Zhong qin Zhang ◽  
Qingqing Zhou ◽  
...  
Keyword(s):  
Quality Control ◽  
Lactic Acid ◽  
Lactobacillus Plantarum ◽  
Microbial Contamination ◽  
Nitrite Accumulation ◽  
Bacteria Growth ◽  
Lactobacillus Plantarum Zj316 ◽  
Stachys Sieboldii

Microbial contamination and nitrite accumulation are two major concerns on the quality control of fermented vegetables. In the present study, a lactic acid bacteria strain Lactobacillus plantarum ZJ316 (ZJ316) was...

Microbial Contamination and Disinfection of Sport Mouthguard: In Vitro Study

2019 ◽  
Vol 77 (2) ◽  
pp. 246-253
Author(s):  
Simonetta D’Ercole ◽  
Marco Tieri ◽  
Diego Martinelli ◽  
Clarissa Ciaravino ◽  
Domenico Fulco ◽  
...  
Keyword(s):  
Microbial Contamination ◽  
In Vitro Study ◽  
Vitro Study

scholarly journals Assessment of microbial contamination of indian currency notes in circulation – An In vitro study

2020 ◽  
Vol 18 (2) ◽  
pp. 179 ◽  
Author(s):  
Sruthi Sunil ◽  
GaneshShenoy Panchmal ◽  
RekhaP Shenoy ◽  
Vijaya Kumar ◽  
Praveen Jodalli ◽  
...  
Keyword(s):  
Microbial Contamination ◽  
In Vitro Study ◽  
Vitro Study

scholarly journals Development of a standardized method to evaluate the protective efficiency of cosmetic packaging against microbial contamination

2020 ◽  
Author(s):  
Chloe Catovic ◽  
Sylvia Martin ◽  
Sephane Desaint ◽  
Christine Borges ◽  
Helene Lesouhaitier ◽  
...  
Keyword(s):  
Adverse Effects ◽  
Microbial Contamination ◽  
Performance Characteristics ◽  
Protective Efficiency ◽  
Cosmetic Product ◽  
And Performance ◽  
The Right ◽  
Preservative Free ◽  
Different Origin

Abstract Doubts surrounding the potential adverse effects of antimicrobial preservatives have modified the demand of consumers, who increasingly insist on the production of low-level and even preservative-free cosmetics. Protection of the product against microbial contamination is therefore focused on the packaging. This has prompted the emergence of a highly diverse array of so-called “protective”, “overprotective”, and “barrier” packaging. However, these designations are not normalized and the choice of the right packaging adapted to each cosmetic product is still essentially empirical, hazardous, and time consuming. The Cosmetic Valleys cluster has launched a commission to define a complete and experimentally-validated method to classify the level of protection of cosmetic packaging against microbial contamination. As reported herein, this required the development a specific bacteriostatic medium that can be used for seven days and an in vitro procedure that reproduces in-use contamination and consumer practices. Based on tests performed on over 800 packages of different origin and performance characteristics, we propose a classification, divided into six grades, to differentiate the protective efficiency of cosmetic packaging. This work can be considered as a first step towards a regulatory text.

scholarly journals Recombinant production and purification of the human protein Tau

2018 ◽  
Vol 31 (12) ◽  
pp. 447-455 ◽  
Author(s):  
Luca Ferrari ◽  
Stefan G D Rüdiger
Keyword(s):  
Escherichia Coli ◽  
Tau Protein ◽  
High Purity ◽  
Affinity Tag ◽  
Protein Tau ◽  
Recombinant Production ◽  
N Terminus ◽  
Cytoskeletal Dynamics ◽  
Robust Protocol

Abstract Tau protein is a microtubule-stabilising protein whose aggregation is linked to Alzheimer’s Disease and other forms of dementia. Tau biology is at the heart of cytoskeletal dynamics and neurodegenerative mechanisms, making it a crucial protein to study. Tau purification, however, is challenging as Tau is disordered, which makes it difficult to produce in recombinant system and is degradation-prone. It is thus challenging to obtain pure and stable preparations of Tau. Here, we present a fast and robust protocol to purify Tau recombinantly in Escherichia coli. Our protocol allows purifying Tau either tag-less or FLAG-tagged at its N-terminus, and Tau fragments of interest. By exploiting a cleavable affinity tag and two anion exchange columns, we obtained Tau preparations of high purity, stable and suitable for in vitro studies, including aggregation experiments that resemble neurodegenerative processes.

scholarly journals Disinfection of Dental Unit Water Line Using Aloe Vera: In Vitro Study

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Sonia Pareek ◽  
Anup Nagaraj ◽  
Prateek Sharma ◽  
Mansi Atri ◽  
Satinder Walia ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Sodium Hypochlorite ◽  
Water Samples ◽  
Microbial Contamination ◽  
Aloe Vera ◽  
Microbiological Quality ◽  
In Vitro Study ◽  
Viable Count ◽  
Dental Unit Waterlines

Context. Dental unit waterlines may be heavily contaminated with microorganisms and are a potential source of infection for both practicing staff and immunocompromised patients particularly. Contamination of dental unit water lines could be inhibited with the use of disinfectants. The present study investigates the effect of aloe-vera-based disinfectant in reducing the microbial growth in dental unit water lines (DUWLs).Aims. To compare the efficacy of aloe vera, hydrogen peroxide (H2O2), and 5% sodium hypochlorite (NaOCl) in controlling microbial contamination of DUWLs.Materials and Methods. After obtaining baseline water samples, the dental unit waterlines were treated with aloe vera, 10% hydrogen peroxide, and 5% sodium hypochlorite. Each of the three disinfectants was used in increasing concentrations and their inhibiting effect was compared. Water samples were analyzed for microbiological quality by the total viable count (TVC) method.Statistical Analysis Used. SPSS 16.Results. There was significant reduction in mean CFU/ml when treated with disinfectants each for a period of one week. Aloe-vera solution was found to be the most effective in reducing the microbial colonies.Conclusions. Improving the water quality from dental unit water lines is of considerable importance; chemical-based disinfectants can be replaced with herbal disinfectants for treating microbial contamination in dental unit waterlines.

scholarly journals Enzymatic biofilm destabilisation to support mechanical cleansing of inserted dental implant surfaces: an in-vitro pilot study

Odontology ◽  
2021 ◽  
Author(s):  
Rutger Matthes ◽  
Lukasz Jablonowski ◽  
Birte Holtfreter ◽  
Christiane Pink ◽  
Thomas Kocher
Keyword(s):  
Shear Stress ◽  
Treatment Outcome ◽  
Pilot Study ◽  
Biofilm Formation ◽  
Microbial Contamination ◽  
Implant Surface ◽  
Other Substances ◽  
Cocamidopropyl Betaine ◽  
Mechanical Cleaning

AbstractPeri-implantitis is caused by microbial contamination and biofilm formation on the implant surface. To achieve re-osseointegration, the microbes must be completely removed from the surface. Adjunctive to mechanical cleaning, chemical treatment with enzymes or other substances could optimise the treatment outcome. Therefore, we investigated the efficacy of different enzymes, a surfactant, and a chelator in destabilising dental polymicrobial biofilm. The biofilm destabilising effect of the glycosidases α-amylase, dextranase, DispersinB®, and lysozyme, as well as the proteinase subtilisin A, and the nuclease Benzonase®, the chelator EDTA, and the surfactant cocamidopropyl betaine were investigated on biofilms, inoculated with plaque on rough titanium discs. The test and the control solutions were incubated for 15 min at 36 °C on biofilms, and loosened biofilm mass was removed by shear stress with a shaker. Fluorescence-stained biofilms were microscopically analysed. Acceptable cell tolerability concentrations of test substances were determined by the MTT (tetrazolium dye) assay on the MG-63 cell line. A statistically significant biofilm destabilising effect of 10% was shown with lysozyme (2500 µg/ml).

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