MiR-203 Mimic Down-Regulates Baculoviral IAP Repeat Containing 5 Expression and Affects Proliferation and Apoptosis of Gastric Cancer Cells
Human baculovirus IAP repeats containing protein 5 (BIRC5) is the most inhibitor of cell apoptosis. Abnormal miR-203 level is associated with the pathogenesis of gastric cancer. Bioinformatics analysis revealed a relationship between miR-203 and BIRC5. Our study assessed miR-203’s role in gastric cancer cells. Tumor tissues and adjacent tissues were collected. miR-203 and BIRC5 mRNA expression in SGC7901 and MKN45 cells was detected by real-time PCR. SGC7901 cells were divided into miR-NC group and miR-203 mimic group followed by analysis of cell proliferation by EdU staining. Compared to adjacent tissues, miR-203 level was decreased and BIRC5 was increased. There was a targeted relationship between miR-203 and BIRC5. Compared with RGM- 1 cells, miR-203 in SGC7901 and MKN45 cells was significantly downregulated and BIRC5 was upregulated. miR-203 mimic significantly downregulated BIRC5 in SGC7901 cells, promoted cell apoptosis, and attenuated cell proliferation. Decreased miR-203 expression and increased BIRC5 expression is associated with the pathogenesis of gastric cancer. MiR-203 can inhibit the expression of BIRC5, inhibit proliferation of gastric cancer cells and induce apoptosis.