A Hypothalamic-Controlled Neural Reflex Promotes Corneal Inflammation

Purpose. To determine time course of effect of lipopolysaccharide (LPS) on production of interleukin-8 (IL-8) and monocyte chemotactic protein (MCP) by cultured human corneal stromal cells.Methods. Human corneal stromal cells were harvested from donor corneal specimens, and fourth to sixth passaged cells were used. Cell cultures were stimulated with LPS for 2, 4, 8, and 24 hours. Northern blot analysis of IL-8 and MCP gene expression and ELISA for IL-8 and MCP secretion were performed. ELISA results were analyzed for statistical significance using two-tailed Student'st-test.Results. Northern blot analysis demonstrated significantly increased IL-8 and MCP gene expression after 4 and 8 hours of exposure to LPS. ELISA for secreted IL-8 and MCP demonstrated statistically significant increases (P<0.05) after corneal stromal cell stimulation with LPS.Conclusions. This paper suggests that human corneal stromal cells may participate in corneal inflammation by secreting potent leukocyte chemotactic and activating proteins in a time-dependent manner when exposed to LPS.

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Expression of Toll-Like Receptor 4 Contributes to Corneal Inflammation in Experimental Dry Eye Disease

Investigative Opthalmology & Visual Science ◽

10.1167/iovs.12-9547 ◽

2012 ◽

Vol 53 (9) ◽

pp. 5632 ◽

Cited By ~ 41

Author(s):

Hyun Soo Lee ◽

Takaaki Hattori ◽

Eun Young Park ◽

William Stevenson ◽

Sunil K. Chauhan ◽

...

Keyword(s):

Dry Eye ◽

Dry Eye Disease ◽

Eye Disease ◽

Toll Like Receptor ◽

Toll Like Receptor 4 ◽

Corneal Inflammation

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Corneal Inflammation Is Inhibited by the LFA-1 Antagonist, Lifitegrast (SAR 1118)

Journal of Ocular Pharmacology and Therapeutics ◽

10.1089/jop.2012.0102 ◽

2013 ◽

Vol 29 (4) ◽

pp. 395-402 ◽

Cited By ~ 18

Author(s):

Yan Sun ◽

Rui Zhang ◽

Thomas R. Gadek ◽

Charles A. O'Neill ◽

Eric Pearlman

Keyword(s):

Corneal Inflammation

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Anti-Apoptotic Properties of Mesenchymal Stem Cells in a Mouse Model of Corneal Inflammation

Stem Cells and Development ◽

10.1089/scd.2020.0195 ◽

2021 ◽

Author(s):

Jan Kossl ◽

Pavla Bohacova ◽

Barbora Hermankova ◽

Eliska Javorkova ◽

Alena Zajicova ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Mouse Model ◽

Corneal Inflammation

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Mechanisms of Peripheral and Central Pain Sensitization: Focus on Ocular Pain

Frontiers in Pharmacology ◽

10.3389/fphar.2021.764396 ◽

2021 ◽

Vol 12 ◽

Author(s):

Giulia Puja ◽

Balazs Sonkodi ◽

Rita Bardoni

Keyword(s):

Central Pain ◽

Spinal Trigeminal Nucleus ◽

Trigeminal Nucleus ◽

Ocular Pain ◽

Cellular Mechanisms ◽

New Techniques ◽

Functional Changes ◽

Corneal Inflammation ◽

Pain Sensitization ◽

Trigeminal Nerves

Persistent ocular pain caused by corneal inflammation and/or nerve injury is accompanied by significant alterations along the pain axis. Both primary sensory neurons in the trigeminal nerves and secondary neurons in the spinal trigeminal nucleus are subjected to profound morphological and functional changes, leading to peripheral and central pain sensitization. Several studies using animal models of inflammatory and neuropathic ocular pain have provided insight about the mechanisms involved in these maladaptive changes. Recently, the advent of new techniques such as optogenetics or genetic neuronal labelling has allowed the investigation of identified circuits involved in nociception, both at the spinal and trigeminal level. In this review, we will describe some of the mechanisms that contribute to the perception of ocular pain at the periphery and at the spinal trigeminal nucleus. Recent advances in the discovery of molecular and cellular mechanisms contributing to peripheral and central pain sensitization of the trigeminal pathways will be also presented.

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EFFECTS OF COWPEA (VIGNA UNGUICULATA) EXTRACT IN VEGF EXPRESSION OF CORNEAL INFLAMMATION RAT MODEL (RATTUS NOVERGICUS STRAIN WISTAR)

Saintika Medika ◽

10.22219/sm.v10i1.4148 ◽

2017 ◽

Vol 10 (1) ◽

pp. 65

Author(s):

Yuliono Trika Nur Hasan ◽

Nanda Wahyu Anandita ◽

Husnul Khotimah

Keyword(s):

Experimental Study ◽

Effective Dose ◽

Vigna Unguiculata ◽

Methanolic Extract ◽

Positive Control ◽

Vascular Endothelial ◽

Vegf Expression ◽

Alkali Burn ◽

Corneal Inflammation ◽

Administration Time

Purpose : to evaluate the effects of Methanolic extract of Cowpea (Vigna unguiculata) administration on vascular endothelial growthfactor (VEGF) expression in rats with corneal-alkali induced inflammation.Methods : This was an interventional experimental study. Sixty three rats were randomly selected, 3 rats as control, 60 rats were treatedwith 1 M NaOH (as inflammation model) and administered with 25 ìM,50 ìM and 100 ìM of genistein in Cowpea (Vignaungucuilata) extract four times daily. Alkali burn by 1 M NaOH infiltration using filter paper applicated on the center cornea of righteye for 60 seconds. Aquabidest as positive control and Cowpea (Vigna ungucuilata) extract treatment perfused immediately after alkali burn.VEGF observed at 6, 24, 48, 96 and 168 hours by immunohistochemical method.Results : Each dose of Cowpea (Vigna unguiculata) extract administration significantly decreased the VEGF expression ( p = 0,00). Differences of VEGF expression based on administration time were not significant (p=0,033). Interaction between time of administrationand dose of administration had influenced the VEGF expression (R Square(r2) =24,7 %). The Linear regression between VEGF expression and dose resulted in estimated effective dose (ED) with Y = 16.486–0.079 X( Y = V E G F e x p r e s s i o n , X =d o s e o f a d m i n i s t r a t i o n ) . Considering VEGF expression in normal cornea was 12, wefound the effective dose of methanolic Vigna unguiculata extract was 61.94 ìM.Conclusion :Methanolic extract of Cowpea(Vigna unguiculata) decreased the VEGF expression on alkali burn corneal inflammationin rats but no differences in VEGF expression based on time administration. Dose of 50 ìM genistein in Methanolic extract ofCowpea (Vigna unguiculata) was close to effective dose 61.94 ìM.Keywords : VEGF expression, alkali burn, Cowpea (Vigna unguiculata) extract, cornea.

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