Differential in situ cytokine gene expression in acute versus chronic atopic dermatitis.

The granulomatous lesions in bovine paratuberculosis have been classified into two types, i.e., the lepromatous type and the tuberculoid type. To clarify the immunopathologic mechanisms at the site of infection, we compared inflammatory cytokine gene expression between the two types of lesions. Samples were obtained from noninfected control cows ( n =5) and naturally infected cows ( n =7) that were diagnosed by enzyme-linked immunosorbent assay (ELISA) and fecal culture test. Although none of the infected cows showed clinical signs, tuberculoid lesions were observed in five cows (tuberculoid group) and lepromatous lesions in two cows (lepromatous group). Among the cytokines examined by reverse transcription-polymerase chain reaction (RT-PCR), Th2-type cytokines interleukin-4 (IL-4) and IL-10, and Th1-type cytokine IL-2 were expressed more significantly in the lepromatous group than in the tuberculoid ( P < 0.01) and noninfected groups ( P < 0.05). No statistical differences were observed in the expression of interferon-gamma, IL-1 beta, TNF-alpha, and GM-CSF among lepromatous, tuberculoid, and noninfected groups. Expression of proinflammatory cytokine IL-12 mRNA, however, did not differ among the three groups; IL-18 was expressed at lower levels in the lepromatous group than in the tuberculoid group and the noninfected group ( P < 0.0001). Moreover, the number of cells in which IL-18 mRNAs were detected by in situ hybridization was markedly decreased in the lepromatous group. These results indicate that the formation of lepromatous-type lesions or tuberculoid-type lesions may be influenced by alterations in Th1/Th2-type cytokine production and that IL-18 may play an important role in a Th1-to-Th2 switch in paratuberculosis.

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Detection of cytokine gene expression in human monocytes and lymphocytes by fluorescent in situ hybridization in cell suspension and flow cytometry.

International Journal of Molecular Medicine ◽

10.3892/ijmm.1.6.995 ◽

1998 ◽

Author(s):

J Wieckiewicz ◽

A Krzeszowiak ◽

I Ruggiero ◽

A Pituch-Noworolska ◽

M Zembala

Keyword(s):

Gene Expression ◽

Flow Cytometry ◽

In Situ Hybridization ◽

Cell Suspension ◽

Fluorescent In Situ Hybridization ◽

Cytokine Gene Expression ◽

Human Monocytes ◽

Cytokine Gene ◽

Monocytes And Lymphocytes

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Analysis of Cytokine Gene Expression using a Novel Chromogenic In-situ Hybridization Method in Pulmonary Granulomas of Cattle Infected Experimentally by Aerosolized Mycobacterium bovis

Journal of Comparative Pathology ◽

10.1016/j.jcpa.2015.06.004 ◽

2015 ◽

Vol 153 (2-3) ◽

pp. 150-159 ◽

Cited By ~ 12

Author(s):

M.V. Palmer ◽

T.C. Thacker ◽

W.R. Waters

Keyword(s):

Gene Expression ◽

In Situ Hybridization ◽

Mycobacterium Bovis ◽

Cytokine Gene Expression ◽

Hybridization Method ◽

Cytokine Gene ◽

Chromogenic In Situ Hybridization ◽

Pulmonary Granulomas

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Suppression of cytokine gene expression and improved therapeutic efficacy of microemulsion-based tacrolimus cream for atopic dermatitis

Drug Delivery and Translational Research ◽

10.1007/s13346-012-0060-8 ◽

2012 ◽

Vol 2 (2) ◽

pp. 129-141 ◽

Cited By ~ 10

Author(s):

Manisha S. Lalan ◽

Naresh C. Laddha ◽

Jigar Lalani ◽

Muhammad J. Imran ◽

Rasheedunnisa Begum ◽

...

Keyword(s):

Gene Expression ◽

Atopic Dermatitis ◽

Therapeutic Efficacy ◽

Cytokine Gene Expression ◽

Cytokine Gene

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In situ islet cytokine gene expression during development of type I diabetes in the non-obese diabetic mouse

Immunology Letters ◽

10.1016/0165-2478(94)90170-8 ◽

1994 ◽

Vol 39 (3) ◽

pp. 283-288 ◽

Cited By ~ 24

Author(s):

H. Toyoda ◽

B. Formby ◽

D. Magalong ◽

A. Redford ◽

E. Chan ◽

...

Keyword(s):

Gene Expression ◽

Type I Diabetes ◽

Diabetic Mouse ◽

Cytokine Gene Expression ◽

Type I ◽

Cytokine Gene

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Local and systemic immune response in patients withsevere atopic dermatitis

Rossiiskii Allergologicheskii ZHurnal ◽

10.36691/rja771 ◽

2011 ◽

Vol 8 (5) ◽

pp. 10-15

Author(s):

T M Filimonova ◽

Ol'ga Gur'evna Elisyutina ◽

E S Fedenko ◽

D D Niyazov ◽

M N Boldyreva ◽

...

Keyword(s):

Gene Expression ◽

Immune Response ◽

Atopic Dermatitis ◽

Peripheral Blood ◽

Transforming Growth Factor ◽

Cytokine Gene Expression ◽

Cytokine Gene ◽

Healthy Individuals ◽

Th2 Response ◽

Healthy Donors

Background. to comparatively investigate cytokine gene expression in the skin and peripheral blood of atopic dermatitis (AD) patients and healthy individuals. Methods. Samples of skin and peripheral blood from 48 severe AD patients SCORAD (Scoring Atopic Dermatitis) 78,5 [57; 89], IGA (Investigators Global Assessment) 4,2 [3,9; 4,7]) at the age of 17 to 45 years and 20 healthy donors aged from 19 to 32 years were analyzed for gene expression of cytokines using real time reverse transcription polymerase chain reaction (RT-PCR). Results. In the skin of patients with AD, a significant increase of the level of gene expression was observed for interleukin IL2R (interleukin) (р=0,0023), IL5 (р=0,002), IL6 (р=0,0023), IL8 (р=0,01), IL12β (р=0,0023), IL10 (р=0,0023), IL23 (р=0,002), IL29 (р=0,0023), and TGFβ (transforming growth factor) (p=0,0023) as compared to healthy individuals. In contrast, no difference between AD patients and healthy donors was detected with respect to cytokine gene expression in the peripheral blood. Conclusions. Activity of IL-2R, IL-8, IL-12β, IL-23, IL-29, and TGFβ that are markers of chronic inflammation and Th1 immune response in severe AD and IL-5, IL-10 that are anti-inflammatory cytokines and markers of Th2 response was predominant in the skin but not in the blood of AD patients.

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