Effect of Ammonia on Nasal Resistance in Atopic and Nonatopic Subjects

1979 ◽  
Vol 88 (2) ◽  
pp. 228-234 ◽  
Author(s):  
J. A. McLean ◽  
K. P. Mathews ◽  
W. R. Solomon ◽  
P. R. Brayton ◽  
N. K. Bayne
Keyword(s):  
Progressive Increase ◽  
Compressed Air ◽  
Nasal Airway ◽  
Nasal Breathing ◽  
Short Term ◽  
Flow Curves ◽  
Simple Method ◽  
Significant Difference ◽  
The Mean ◽  
Small Change

Nasal airway resistance (NAR) was assessed from the slope of pressure-flow curves obtained during normal nasal breathing. Volunteers were classified as atopic or nonatopic according to strict criteria. 100 ppm NH3 was introduced into each nostril for periods ranging from 5 to 30 seconds with frequent NAR monitoring. A progressive increase in NAR responses was obtained with incremental NH3 exposures, but no significant difference was noted between the mean response of atopic and nonatopic subjects. Control exposures to compressed air under the same pressure generally produced only a small change in NAR, while aerosolized buffered saline increased NAR more than compressed air. The nasal response to NH3 was effectively inhibited by intranasal atropine administration but not by chlorpheniramine. The described procedure provides a safe and simple method for studying semiquantitatively the short-term effects of inhaled irritants on the nose.

A COMPARISON OF TWO METHODS FOR MEASUREMENT OF ALDOSTERONE SECRETION RATE IN MAN

1966 ◽  
Vol 53 (2) ◽  
pp. 177-188 ◽  
Author(s):  
P. Lund-Johansen ◽  
T. Thorsen ◽  
K. F. Støa
Keyword(s):  
Urinary Excretion ◽  
Normal Subjects ◽  
Secretion Rate ◽  
Aldosterone Secretion ◽  
Simple Method ◽  
Mean Values ◽  
Pathological Conditions ◽  
Significant Difference ◽  
Derivative Method ◽  
The Mean

ABSTRACT A comparison has been made between (A), a relatively simple method for the measurement of aldosterone secretion rate, based on paper chromatography and direct densitometry of the aldosterone spot and (B) a more elaborate isotope derivative method. The mean secretion rate in 9 normal subjects was 112 ± 26 μg per 24 hours (method A) and 135 ± 35 μg per 24 hours (method B). The »secretion rate« in one adrenalectomized subject after the intravenous injection of 250 μg of aldosterone was 230 μg per 24 hours (method A) and 294 μg per 24 hours (method B). There was no significant difference in the mean values, and correlation between the two methods was good (r = 0.80). It is concluded that the densitometric method is suitable for clinical purposes as well as research, being more rapid and less expensive than the isotope derivative method. Method A also measures the urinary excretion of the aldosterone 3-oxo-conjugate, which is of interest in many pathological conditions. The densitometric method is obviously the less sensitive and a prerequisite for its use is an aldosterone secretion of 20—30 μg per 24 hours. Lower values are, however, rare in adults.

Assessment of Safety and Applicability of Laparoscopic vs Open Non Anatomical Resection of Hepatocellular Carcinoma in Cirrhotic Patients

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Mohammed Mahfouz Mohammed ◽  
Hany Saeed Abdel Basset ◽  
Mohammed Abd Almegeed Elsayed ◽  
Ahmed Abdel Basset Hegazi
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Resection ◽  
Laparoscopic Liver Resection ◽  
Operative Time ◽  
Short Term ◽  
Open Liver Resection ◽  
Resection Group ◽  
Significant Difference ◽  
Cirrhotic Patients ◽  
The Mean

Abstract Background Laparoscopic liver resection (LLR) has been progressively developed along the past two decades. Liver surgery was one of the last frontiers reached by minimally invasive surgery. Surgical technique and equipment evolved to overcome technical limitations, making laparoscopic liver resections (LLR) safe and feasible. Surgeons developed skills in a stepwise approach, beginning with low complexity operations for benign diseases and reaching high-complexity surgeries for malignant cases and living donor organ harvesting. Objective s: The aim of the study is to compare short term results of laparoscopic versus open hepatectomy regarding to intra operative details and post-operative management and complications for achieving a safe hepatic resection for treatment of HCC in cirrhotic patients. Patients and Methods In this prospective study, a comparison between laparoscopic resection and open resection was done to compare short-term results between laparoscopic and open liver resection. This study was conducted on 30 patients with hepatocellular carcinoma. 15 patients (50%) were treated by laparoscopic liver resection (Group A) while the other 15 patients (50%) were treated by open liver resection (Group B). Results Regarding the demographic data, the presence of past history of medical condition and the preoperative laboratory results, no statistical significance was found. The mean operative time has statistically significant difference between the 2 groups, with decreased operative time in the laparoscopic group (P < 0.001). The mean blood loss has no statistically significant difference relations between the 2 groups, (P = 0.866) with conversion rate of (13.3%) happened in two cases. Conclusion Laparoscopic liver resection is a safe and feasible treatment option for HCC in cirrhotic patient needing minor resection at laparoscopic segments (II, III, IVa,V,VI). Laparoscopic liver resection for HCC has superior short- term and comparable oncological outcomes to open liver resection. LLR should be performed for carefully selected patients and by an expert surgical team.

The effect of associate perennial grasses on a simple pasture mixture of phalaris and subterranean clover

1956 ◽  
Vol 7 (5) ◽  
pp. 367 ◽  
Author(s):  
WD Andrew ◽  
CA Neal-Smith
Keyword(s):  
Festuca Arundinacea ◽  
Perennial Grass ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Yield Component ◽  
Perennial Grasses ◽  
Grass Species ◽  
Significant Difference ◽  
The Mean ◽  
Small Change

Over the period 1952-1954 there was no significant difference in the yield of herbage produced annually by the addition to a Phalaris tuberosa L.–Trifolium subterraneum L. pasture mixture, of any one of the following grasses: Agropyron obtusiusculum Lange., Bromus coloratus Steud., Bromus inermis Leyss., Dactylis glomerata L., or Festuca arundinacea Schreb. There were indications of a small change in seasonal production where certain grasses, notably D. glomerata, were included in the mixture. Over the 3-year period the proportion of the sown grass component, in the mixtures where either D. glomerata, B. inermis, or B. coloratus were included, increased by a greater amount than where the simple mixture of phalaris and subterranean clover was used. The addition of each grass also lessened the amount of P. tuberosa in the sown grass component of the yield. In the third year, despite the varying proportions of the phalaris and associated sown grass species, the mean population of the sown perennial grasses in each treatment did not differ significantly from the mean figure of 1.34 plants/sq. lk. The increased production of the sown grass yield component following the association of certain of the above species with P. tuberosa suggests that the latter does not fully exploit the environment. The principle of including another perennial grass when sowing phalaris and subterranean clover might have wide application as a means of combatting "phalaris staggers".

306 ANALYSIS OF GLOBAL DNA METHYLATION IN BOVINE SPERMATOZOA

2006 ◽  
Vol 18 (2) ◽  
pp. 260
Author(s):  
H. Kato ◽  
M. Kishimoto ◽  
T. Mitani ◽  
K. Matsumoto ◽  
K. Saeki ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Species Difference ◽  
Restriction Enzymes ◽  
Area Measurement ◽  
Global Dna Methylation ◽  
Simple Method ◽  
Sperm Dna ◽  
Significant Difference ◽  
Bovine Spermatozoa ◽  
The Mean

Methylation of cytosine base in the CpG dinucleotide sequence (DNA methylation) is a major epigenetic modification of the genome and plays an important role in gene expression. Recently, global DNA methylation in genome was studied by using a restriction landmark genomic scanning (RLGS) method and/or a representational difference analysis (RDA) method. However, these methods are complicated and need to use restriction enzymes. Therefore, the information derived from those methods is restricted to the region of the DNA sequence which is able to be cleaved by restriction enzymes. In this study, to establish a simple method to estimate global DNA methylation level in bovine spermatozoa, we tried to develop the DNA methylation analyzing method by using immunostaining of 5-methylcytosine. The immunostaining method for 5-methylcytosine in this study was based on the method developed by Benchaib et al. (2003 Fertil. Steril. 80, 947-952) for human spermatozoa. Because of the species difference, we modified some treatments to apply to bovine spermatozoa. Frozen-thawed bovine spermatozoa were washed by using 30 and 45% Percoll gradient solutions. After washing, spermatozoa were treated with 0.25 dithicthreitol M (DTT) and 1% sodium dodecyl sulfate (SDS) at room temperature (RT). Then, treated spermatozoa were spread on a slide glass with Cytospin4 (30g, 5 � 104 cells/mL) and air-dried at RT. Air-dried bovine spermatozoa specimens were fixed in methanol: glacial acetic acid (3:1) solution at RT and treated with 1% Triton X and 1% SDS at RT; DNA was denatured with 6 N HCl at RT. After the denaturation, 5-methylcytosine in sperm DNA was analyzed by immunofluorescence technique with mouse anti 5-methylcytosine antibody and fluorescein isothiocyanate (FITC)-conjugated goat anti mouse IgG antibody. The total sperm DNA was counterstained with propidium iodide (PI). Stained samples were observed with a confocal laser scanning microscope and obtained images were analyzed with fluorescence image analysis software. The area that was clearly stained with PI in each sperm head was designated and measured as the area of total sperm DNA, and the number of the dots that showed FITC fluorescence within the total sperm DNA area was designated and measured as the area of 5-methylcytosine in total sperm DNA. The area measurement was performed with fixed light strength. Three bovine spermatozoa samples derived from different bulls, used daily for calf production by AI, were examined. The ratio of the mean total area of the 5-methylcytosine in sperm DNA to the mean total area of the sperm DNA was 34.1% in bull A (9.13 � 5.66 �m2, 26.75 � 5.29 �m2, n = 57), 45.2% in bull B (16.60 � 3.79 �m2, 36.74 � 5.95 �m2, n = 41) and 43.9% in bull C (14.66 � 4.27 �m2, 33.45 � 7.13 �m2, n = 22). There was significant difference in the ratio between bull A and bulls B and C (P < 0.01). More research is required to evaluate the meaning of this individual difference of DNA methylation between bulls. This work was supported by Wakayama Prefecture Collaboration of Regional Entities for the Advanced of Technological Excellence, Japan, and by a Grant-in-Aid for the 21st Century Center of Excellence Program of the MEXT, Japan.

Performance and work of nasal breathing

2021 ◽  
Vol 11 (41) ◽  
pp. 11-17
Author(s):  
Anita Bergmane ◽  
Klaus Vogt ◽  
Biruta Sloka
Keyword(s):  
Airway Resistance ◽  
Interquartile Range ◽  
Nasal Airway ◽  
Nasal Resistance ◽  
Nasal Breathing ◽  
First Line ◽  
Significant Difference ◽  
Before And After ◽  
Potential Parameters ◽  
Inspiratory Work

Abstract OBJECTIVE. To evaluate performance (Q) and work (W) of nasal breathing as potential parameters in functional diagnostic of nasal obstruction. MATERIAL AND METHODS. We included in our study 250 patients and we measured by 4-phase-rhinomanometry with decongestion test. We calculated performance Q of the “representative breath” in inspiration and expiration and in total breath, maximal performance Q (Qmax), Work W of nasal breathing in mJ and in mJ/litre and Q in J/min. RESULTS. The interquartile range of Win for representative breath before decongestion is 356 mJ/l, Wex 308 mJ/l, while after decongestion Win is 264 mJ/l and Wex 220 mJ/l. There is no significant difference between work before and after decongestion (p<0.001). Interquartile range for nasal breathing Q before decongestion is 19.2 J/min and after – 14.3 J/min. A significant correlation exists between logarithmic vertex resistance for inspiration and expiration and Qmax for inspiration and expiration (p<0.001). That means that the performance required by breathing depends in the first line on nasal resistance. CONCLUSION. Inspiratory work is 1.2 times higher than expiration work. Increase in nasal airway resistance is followed by increase in maximal nasal performance.

Spreader grafts: functional or just aesthetical?

2015 ◽  
Vol 53 (4) ◽  
pp. 332-339
Author(s):  
R. Xavier ◽  
S. Azeredo-Lopes ◽  
A. Papoila
Keyword(s):  
Functional Improvement ◽  
Nasal Airway ◽  
Nasal Airflow ◽  
Functional Effect ◽  
Nasal Breathing ◽  
The Mean ◽  
The Difference ◽  
The Aesthetic ◽  
Peak Nasal Inspiratory Flow

Objective: Spreader grafts are commonly used in rhinoplasty to achieve an aesthetic improvement of the nose or a functional improvement of the nasal airway. Currently, the aesthetic role of spreader grafts is well established. The functional effect of these grafts, however, has been controversial due to the lack of studies clearly demonstrating an increase on nasal airflow assigned to spreader grafts. The purpose of this study is to evaluate the effect of spreader grafts on nasal breathing. Methods: Nasal breathing of 72 consecutive patients undergoing rhinoplasty was evaluated by measuring peak nasal inspiratory flow (PNIF) before surgery and six months after surgery. Results: The mean preoperative PNIF of the 72 patients included in this study was 79.44 l/min and the mean postoperative PNIF was 110.42 l/min (p < 0.001). In 37 patients of this study no spreader grafts were used. In this group of patients the mean PNIF values changed from 73.24 l/min before surgery to 99.46 l/min after surgery. In the group of 35 patients in whom spreader grafts were used the mean PNIF values changed from 86.00 l/min before surgery to 122.00 l/min after surgery. The increase in the mean PNIF value after rhinoplasty was slightly higher in the group of patients with spreader grafts than in the group of patients without spreader grafts. The difference in the postoperative increase of PNIF between these two groups of patients, however, is not statistically significant. Conclusions: This study suggests that patients undergoing rhinoplasty have a statistically significant improvement in nasal breathing after surgery. However, patients receiving spreader grafts in a non-randomized way do not have statistically significant greater benefit than those who do not.

Abstract 18183: Short-term Impact of Defibrillation Testing on Heart Failure Admission

Circulation ◽  
2015 ◽  
Vol 132 (suppl_3) ◽  
Author(s):  
Baris Akdemir ◽  
Balaji Krishnan ◽  
Venkatakrishna Tholakanahalli ◽  
David Benditt ◽  
Jian-Ming Li
Keyword(s):  
Heart Failure ◽  
Retrospective Study ◽  
Short Term ◽  
Primary Endpoints ◽  
Significant Difference ◽  
Heart Failure Admission ◽  
The Mean ◽  
Demographic Features ◽  
Long Term Mortality

Introduction: SIMPLE trial showed that defibrillation testing (DT) is safe but does not improve shock efficacy or mortality. However, impact of DT on heart failure (HF) admission at 30 days is unclear. Objectives: The aim of this study was to compare mortality and HF admission of ICD recipients who underwent DT (DT+) compared with those who did not (DT-). Methods: In this retrospective study, we analyzed consecutive patients who received an ICD ( new implant or generator change ) with DT+ and DT- between January 2008 and May 2014 from our ICD registry. Primary endpoints were death ( 30 days and 1 year ) and HF admission ( 30 days) . Results: Of the 501 patients, 311 patients (62,1%) were in DT+ group vs 190 (37,9%) were in DT- group. The mean ages in DT+ and DT- were 66 ± 10 and 70 ± 10 respectively (p<0.0001). DT- group had more generator change than DT+ group ( 61,1% versus 30,9%, p < 0.0001). Other demographic features were not significantly different between two groups (Table 1). No significant difference in mortality was found between two groups at 30 days and 1 year. HF admission at 30 days was significantly higher in DT+ group than in DT- group ( 17,4 % versus 4,7%, p < 0.0001) (Table 2). Conclusion: No short-term and long-term mortality were associated with DT, but DT was associated with increased HF admission at 30 days. Future prospective studies are needed to prove this association.

scholarly journals The Influence of Adipose-derived Stromal Vascular Fraction Cells on the Treatment of Knee Osteoarthritis

2020 ◽  
Author(s):  
Masanori Tsubosaka ◽  
Tomoyuki Matsumoto ◽  
Satoshi Sobajima ◽  
Takehiko Matsushita ◽  
Hideki Iwaguro ◽  
...  
Keyword(s):  
Knee Osteoarthritis ◽  
T2 Mapping ◽  
Stromal Vascular Fraction ◽  
Clinical Effects ◽  
Short Term ◽  
Cell Injection ◽  
Knee Oa ◽  
Significant Difference ◽  
The Mean ◽  
Better Than

Abstract Background Adipose-derived stromal vascular fraction (SVF) cells are a mixed cell population that includes cells with multilineage potential, similar to bone marrow-derived mesenchymal stem cells. Our purpose is to investigate the influence of SVF cells in patients with knee osteoarthritis (OA) and the short-term treatment effects. Methods Fifty-seven patients were enrolled and treated with intra-articular injection of 5.0×10 7 SVF cells into the knee joint between September 2017 and March 2018. All patients were followed up for 12 months or longer. Mean age at treatment and follow-up period were 69.4±6.9 years and 13.7±2.0 months, respectively. The mean preoperative HKA angle was 6.7±3.6°. SVF cells were prepared using the Celution®800/CRS system from the patients’ abdominal or breech subcutaneous fat. The mean SVF cell viability was 90.6±2.7%. Clinical evaluations were performed for range of motion, Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), visual analog scale (VAS) for pain, and the Knee injury and Osteoarthritis Outcome Score (KOOS). Imaging evaluations, which included the HKA angle assessed via radiography, and T2 mapping value using a 3.0-T magnetic resonance imaging unit were also assessed. Both clinical and imaging evaluations were performed preoperatively, 1, 3, 6, and 12 months postoperatively, and compared among all timepoints (p<0.05). Results Knee extension angle at 6 and 12 months postoperatively was significantly better than the preoperative angle. Total WOMAC, VAS, and KOOS scores at 1, 3, 6 and 12 months postoperatively were significantly better than preoperative scores. There was no significant difference in HKA angle among the five time periods. T2 mapping values of lateral femur and tibia were significantly higher 12 months postoperatively than preoperatively. Conclusions The short-term clinical effects of intra-articular SVF cell injection on knee OA were excellent. Intra-articular SVF cell injection is a novel and innovative approach for treating patients with knee OA.

Some Studies on the Polyethylene Glycol Turbidity Method for Detecting Immune Complexes in Serum

1981 ◽  
Vol 18 (6) ◽  
pp. 373-377 ◽  
Author(s):  
D C Kilpatrick ◽  
J Weston ◽  
W J Irvine
Keyword(s):  
Polyethylene Glycol ◽  
Immune Complexes ◽  
Solid Phase ◽  
Mean Value ◽  
Simple Method ◽  
Significant Difference ◽  
The Mean ◽  
Turbidity Method ◽  
Polyethylene Glycol Precipitation ◽  
Routine Assay

A rapid and simple method for detecting circulating immune complexes based on turbidity measurements following polyethylene glycol precipitation was studied with regard to its suitability as a routine assay in clinical laboratories. This method was found to have an acceptable degree of precision provided the temperature was carefully controlled. The mean value obtained with a group of 70 blood donors was 0·09 (SD 0·05) and the 90th percentile value was 0·16. There was no significant difference between values obtained from groups divided on the basis of age or sex. Of 70 diabetic sera assayed by the polyethylene glycol turbidity method, 20% gave positive values although only 10% were strongly positive. The corresponding figures for the solid phase Clq binding method were 15·7% and 14·3%, respectively. Correlation between the two methods was poor. It was concluded that although both methods have a similar likelihood of detecting immune complexes in randomly selected diabetics, it is probable that different immune complexes were being detected.

scholarly journals Short-Term Effects of Hydrokinesiotherapy in Hospitalized Preterm Newborns

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Welcy Cassiano de Oliveira Tobinaga ◽  
Cirlene de Lima Marinho ◽  
Vera Lucia Barros Abelenda ◽  
Paula Morisco de Sá ◽  
Agnaldo José Lopes
Keyword(s):  
Heart Rate ◽  
Oxygen Saturation ◽  
Respiratory Rate ◽  
Salivary Cortisol ◽  
Short Term ◽  
Peripheral Oxygen Saturation ◽  
Significant Difference ◽  
The Mean ◽  
Preterm Newborns ◽  
Short Term Effects

Background. In the neonatal intensive care unit (NICU) environment, preterm newborns are subject to environmental stress and numerous painful interventions. It is known that hydrokinesiotherapy promotes comfort and reduces stress because of the physiological properties of water.Objective. To evaluate the short-term effects of hydrokinesiotherapy on reducing stress in preterm newborns admitted to the NICU.Materials and Methods. Fifteen preterm newborns underwent salivary cortisol measurement, pain evaluation using the Neonatal Infant Pain Scale (NIPS), and heart rate, respiratory rate, and peripheral oxygen saturation measurements before and after the application of hydrokinesiotherapy.Results. The mean gestational age of the newborns was34.2±1.66weeks, and the mean weight was1823.3±437.4 g. Immediately after application of hydrokinesiotherapy, a significant reduction was observed in salivary cortisol(p=0.004), heart rate(p=0.003), and respiratory rate(p=0.004)and a significant increase was observed in peripheral oxygen saturation(p=0.002). However, no significant difference was observed in the NIPS score(p>0.05).Conclusion. In the present study, neonatal hydrotherapy promoted short-term relief from feelings of stress. Neonatal hydrokinesiotherapy may be a therapeutic alternative. However, this therapy needs to be studied in randomized, crossover, and blinded trials. This trial is registered withNCT02707731.

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