scholarly journals Thyroxine Radioimmunoassay for Population Surveys Using Dried Blood: Modifications of a Highly Sensitive Method

1982 ◽  
Vol 19 (1) ◽  
pp. 29-34 ◽  
Author(s):  
María Jesús Obregón
Keyword(s):  
Polyethylene Glycol ◽  
Thyroid Function ◽  
Filter Paper ◽  
Reference Method ◽  
Population Surveys ◽  
Serum Thyroxine ◽  
Highly Sensitive ◽  
Polyethylene Glycol 6000 ◽  
Sensitive Method

A highly sensitive radioimmunoassay for the determination of thyroxine in blood spotted on filter paper is described. The radioimmunoassay developed by Weeke and Ørskov for determination of serum thyroxine was simplified and shortened, and its cost was decreased, for use in programmes for mass screening of thyroid function. The method permits the use of small (2 mm diameter) dried blood dots, thus avoiding interference by binding proteins. Polyethylene glycol 6000 is used for the final separation of antibody bound labelled thyroxine, instead of the expensive and time-consuming wick chromatography used in the reference method. Serum thyroxine concentrations obtained using dried blood by the modified procedure correlate well with those obtained using serum by the reference method. The distribution of serum thyroxine concentration for a population of 2134 babies is given.

scholarly journals Newly Developed Highly Sensitive Method for the Determination of Capecitabine by Using UV-Spectroscopy

2019 ◽  
Vol 11 (03) ◽  
Author(s):  
Manas Ranjan Mishra ◽  
Punam Agrawal ◽  
Surya Narayan Das
Keyword(s):  
Colon Cancer ◽  
Uv Spectroscopy ◽  
First Line ◽  
Spectrophotometric Methods ◽  
Advanced Colon Cancer ◽  
Ich Guidelines ◽  
Highly Sensitive ◽  
Solvent Systems ◽  
Sensitive Method

Capecitabine is a 'pro-drug' to the cytotoxic agent 5-fluorouracil (5-FU) intended to administered orally. Capecitabine is generally used as first line monotherapy for advanced colon cancer. Simple, rapid, accurate UV spectrophotometric methods were developed in the present study and validated for the estimation of Capecitabine in bulk and its formulations as per ICH guidelines. Three solvent systems viz., 0.1N NaOH, 0.1N HCl and Methanol: Water (1:3) was tried. The results suggest that the developed method shows linearity over the range of concentration 2-24μg/ml and a correlation coefficient of 0.9999. Accuracy, precision, linearity, robustness, and ruggedness were statistically validated as per ICH guidelines for all the developed methods. The % RSD values for validated methods were found to be less than 1.5 and methods will find application in routine analysis of drug formulations containing Capecitabine.

A highly sensitive method for quantification of iohexol

2014 ◽  
Vol 6 (11) ◽  
pp. 3706-3712 ◽  
Author(s):  
Anna Schulz ◽  
Falko Boeringer ◽  
Janine Swifka ◽  
Axel Kretschmer ◽  
Mandy Schaefer ◽  
...  
Keyword(s):  
Chronic Kidney Disease ◽  
Glomerular Filtration Rate ◽  
Kidney Disease ◽  
Glomerular Filtration ◽  
Filtration Rate ◽  
Accurate Determination ◽  
Highly Sensitive ◽  
Sensitive Method

Iohexol (1-N,3-N-bis(2,3-dihydroxypropyl)-5-[N-(2,3-dihydroxypropyl) acetamide-2,4,6-triiodobenzene-1,3-dicarboxamide) is used for accurate determination of the glomerular filtration rate (GFR) in chronic kidney disease (CKD) patients.

Determination of Total Urinary Protein, Combining Lowry Sensitivity and Biuret Specificity

1973 ◽  
Vol 19 (10) ◽  
pp. 1170-1178 ◽  
Author(s):  
Karl Doetsch ◽  
Richard H Gadsden
Keyword(s):  
Ion Exchange ◽  
Copper Complex ◽  
Biological Fluids ◽  
Gel Filtration ◽  
Urinary Protein ◽  
Peptide Bonds ◽  
Highly Sensitive ◽  
Filtration Step ◽  
Sensitive Method

Abstract A highly sensitive method with specificity for the peptide chain backbone was developed for determination of total urinary protein. Interfering substances are removed by gel filtration and cupric ions are stoichiometrically bound to the peptide bonds of protein by the biuret reaction. Ion-exchange characteristics of the gel are neutralized, allowing protein—copper complex to be separated from excess cupric ions by a second gel filtration step. Copper bound to peptide bonds is colorimetrically determined by use of sodium diethyldithiocarbamate. Nonprotein substances do not interfere unless they simultaneously bind to protein and chelate copper. As little as 1 mg of total protein per deciliter can be determined in heterogeneous biological fluids such as urine.

scholarly journals Method for the determination of immune complexes by reaction with polyethylene glycol

1982 ◽  
Vol 63 (2) ◽  
pp. 10-13
Author(s):  
B. A. Molotilov ◽  
A. N. Mayansky ◽  
N. D. Pozdnyak ◽  
L. Ch. Samerkhanova
Keyword(s):  
Polyethylene Glycol ◽  
Lupus Erythematosus ◽  
Immune Complexes ◽  
Clinical Laboratory ◽  
Circulating Immune Complexes ◽  
Healthy Donors ◽  
Highly Sensitive ◽  
Photoelectric Colorimeter ◽  
Health And Disease

A study of circulating immune complexes was carried out using a reaction with polyethylene glycol. The method turned out to be simple, highly sensitive and affordable for any clinical laboratory with a photoelectric colorimeter. Analysis of the survey data of 115 healthy donors, 63 patients with rheumatoid arthritis and 16 patients with systemic lupus erythematosus made it possible to establish the level of circulating immune complexes in health and disease. The circulating immune complexes were studied in patients with rheumatism and chronic tonsillitis. To assess the results of the reaction, human aggregated gamma globulin (manufactured by Kazan NIIEM) was used.

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