Levels of haemolysis have no effect on femoral vein post-mortem tryptase levels

2021 ◽  
pp. 002580242199885
Author(s):  
Jack Garland ◽  
Benjamin Ondruschka ◽  
Ugo Da Broi ◽  
Cristian Palmiere ◽  
Charley Glenn ◽  
...  
Keyword(s):  
Sampling Method ◽  
Femoral Vein ◽  
Routine Practice ◽  
Post Mortem ◽  
Sample Collection ◽  
Blood Samples ◽  
Future Studies ◽  
Tryptase Level ◽  
The Mean ◽  
The Relationship

Haemolysis is reported to be an artefact that may alter post-mortem tryptase levels. However, previous studies did not sample peripheral blood using newly standardised methods. Recent studies have shown that some previously recognised peri- and post-mortem confounders can be muted by careful sample collection with first clamping and then sampling the femoral vein. This prospective study investigated the relationship between the degree of haemolysis of the blood samples and femoral vein post-mortem tryptase levels when sampled using this recommended method. Seventy consecutive post-mortem tryptase levels in non-anaphylactic deaths were compared to the degree of haemolysis of these samples, and results showed no significant correlation between them. The mean post-mortem tryptase level was 9.5 μg/L. This study demonstrated that the effects of haemolysis on femoral vein post-mortem tryptase was negligible when the blood was sampled using the recommended sampling method. Future studies on post-mortem tryptase as well as other typically used blood markers in forensics are recommended to adopt this method of blood sampling in routine practice.

Association between obstructive sleep apnea and persistent-postural perceptual dizziness

2021 ◽  
pp. 1-6
Author(s):  
Anand K. Bery ◽  
Jayson Lee Azzi ◽  
Andre Le ◽  
Naomi S. Spitale ◽  
Judith Leech ◽  
...  
Keyword(s):  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Sensitivity And Specificity ◽  
Berlin Questionnaire ◽  
Future Studies ◽  
Obstructive Sleep ◽  
Chronic Dizziness ◽  
Patients At Risk ◽  
The Mean ◽  
The Relationship

BACKGROUND: Obstructive sleep apnea (OSA) has been linked to vestibular dysfunction, but no prior studies have investigated the relationship between Persistent Postural Perceptual Dizziness (PPPD), a common cause of chronic dizziness, and OSA. OBJECTIVE AND METHODS: We determined the frequency of OSA in an uncontrolled group of PPPD patients from a tertiary dizziness clinic based on polysomnogram (PSG). We then assessed the sensitivity and specificity of common OSA questionnaires in this population. RESULTS: Twenty-five patients with PPPD underwent PSG (mean age 47, 60% female, mean BMI 29.5). A majority, or 56%, of patients were diagnosed with OSA, and in most, the OSA was severe. OSA patients were older (56 years versus 40 years, p = 0.0006) and had higher BMI (32 versus 26, p = 0.0078), but there was no clear gender bias (56% versus 64% female, p = 1.00). The mean sensitivity and specificity of the STOP BANG questionnaire for detecting OSA was 86% and 55%, respectively. Sensitivity and specificity of the Berlin Questionnaire was 79% and 45%, respectively. CONCLUSIONS: The prevalence of OSA was much higher in our small PPPD group than in the general population. Screening questionnaires appear to demonstrate good sensitivity to detect PPPD patients at risk of OSA in this small study. Future studies should confirm these findings and determine whether treatment of OSA improves symptoms in PPPD.

scholarly journals Education and Behavioural Barriers for Menstrual Health Communication between Parent and Adolescent Girls

2021 ◽  
Vol 9 (6) ◽  
Author(s):  
Suma K G
Keyword(s):  
Adolescent Girls ◽  
Educational Level ◽  
Sampling Method ◽  
Parental Communication ◽  
Chi Square ◽  
Descriptive Research ◽  
Menstrual Health ◽  
Chi Square Test ◽  
The Mean ◽  
The Relationship

This study is an empirical attempt to understand parental communication about bleeding, first period and dysmenorrhea with adolescent girls. It examines the barriers of communication from the adolescent girls' perspective. The study was conducted in a couple of taluks of ballari district, i.e., Sandur and Hospet. In order to achieve the objectives of the study, a descriptive research design was adopted. The data was gathered using the interview schedule from 260 adolescent girls adopting a random sampling method. The findings of the study reveal that the mean age of the adolescent girls was 15.34 years. It was found that the parents' behaviour and educational level impact communication with adolescent girls with reference to bleeding, first period and dysmenorrhea. The Chi-Square test has been done to assess the relationship between communicational barriers and education. The ANOVA test has been applied to understand the relationship between parents' behaviour, educational level and communication barriers.

scholarly journals The relationship between adaptability and life satisfaction among ostomy patients

2019 ◽  
Author(s):  
Fazel Dehvan ◽  
Monir Nobahar ◽  
Raheb Ghorbani ◽  
Reza Ghanei Gheshlagh ◽  
Masoomeh Najafi ◽  
...  
Keyword(s):  
Life Satisfaction ◽  
Sampling Method ◽  
Satisfaction With Life ◽  
Satisfaction With Life Scale ◽  
Demographic Information ◽  
Continuous Sampling ◽  
Life Scale ◽  
The Mean ◽  
The Relationship ◽  
Functional Problems

Background & Aim: Ostomy surgery is used to provide a means of collecting waste through a channel. However, ostomy can produce functional problems and major changes in adaptability and life satisfaction among the patients. This study attempts to investigate the relationship between adaptability and life satisfaction among ostomy patients. Methods & Materials: This study is descriptive-correlational. 100 ostomy patients referring to Iran Ostomy Association were selected and studied using continuous sampling method and questionnaires of demographic information, Satisfaction with Life Scale (SWLS), and Bell Adjustment Inventory (BAI). Results: The mean ± standard deviation of life satisfaction scores and adaptability scores were 16.8±4.4 (of 35) and 53.1±23.7, respectively. A positive and significant correlations were observed between life satisfaction and adjustment (r=0.597, p<0.001), and also adjustment dimensions, including home (r=0.585, p<0.001), health (r=0.611, p<0.001), social (r= 0.460, p<0.001), emotional (r=0.538, p<0.001) and occupational adjustment (r=0.380, p<0.001). Conclusion: According to the results, there was a positive and significant relationship between life satisfaction and adaptability. Thus, therapeutic department members have to help improve life satisfaction among ostomy patients by facilitating adaptability.

İş Yaşamında Yalnızlığın Örgütsel Ses Üzerindeki Etkisi

2021 ◽  
Vol 7 (13) ◽  
pp. 471-483
Author(s):  
Ümit Şevik
Keyword(s):  
Sampling Method ◽  
Negative Relationship ◽  
Future Studies ◽  
Organizational Silence ◽  
Emotional Deprivation ◽  
Convenience Sampling ◽  
Prosocial Voice ◽  
Social Companionship ◽  
Voice And Silence ◽  
The Relationship

Organizational voice and silence are multidimensional concepts located at two different poles. While there are studies in the literature examining the relationship between organizational silence and loneliness, there are not enough studies on the relationship between organizational voice and loneliness. Within the scope of the “exit, voice and loyalty” model of Hirschman (1970), employees give various reactions to negativities. In this study, the effects of loneliness in the workplace on organizational voice were examined within the scope of the responses of the employees. In line with the purpose of the study, a questionnaire was applied to 330 employees working in Ankara using the convenience sampling method. In the study, "Workplace Loneliness Scale" and "Organizational Voice Scale" were used. Within the scope of the study, the data were analyzed using SPSS 24 and AMOS 24 programs. It was determined that the fit values of the model (CMIN\DF=2.192; GFI=0.879; AGFI=0.848; IFI=0.927; RMSEA=0.060) were found to be between acceptable values. As a result of the analyzes, the emotional deprivation sub-dimension has a positive relationship on the defensive voice (β=.624, p<0.001), while social companionship sub-dimension has a negative relationship on the acquiescent voice (β=-.434, p<0.001) and prosocial voice (β = -.787, p<0.001). In addition, significant differences between variables according to demographic characteristics were examined and recommendations were made for future studies. Key Words: Loneliness in the Workplace, Organizational Voice

scholarly journals The prevalence of HIV in the sudden, unexplained and unexpected death population at the Pretoria Medico-Legal Laboratory

2016 ◽  
Vol 17 (1) ◽  
Author(s):  
Neil K. Morris ◽  
Lorraine Du Toit-Prinsloo ◽  
Lynne Webber ◽  
Gert Saayman
Keyword(s):  
South Africa ◽  
Respiratory Disease ◽  
Hiv Prevalence ◽  
Post Mortem ◽  
Sample Collection ◽  
Forensic Pathologist ◽  
Unexpected Death ◽  
Blood Samples ◽  
Hiv Antibodies

Purpose: To determine the prevalence of HIV in the sudden, unexplained and unexpected (SUU) death population admitted to the Pretoria Medico-Legal Laboratory.Methods: This study was conducted at the Pretoria Medico-Legal Laboratory. Blood samples were obtained from decedents who died suddenly and/or unexpectedly, during autopsy, by a forensic pathologist. Sample collection continued until 100 valid samples were analysed for HIV antibodies. The data collected included demographic details and case-related information.Results and Conclusion: SUU deaths accounted for 14% of all cases admitted to the Pretoria Medico-Legal Laboratory. The HIV prevalence in the SUU deaths was 43%, which is 17% higher than the general mortuary population in Pretoria ( p = 0.0045). The majority of these deaths were due to respiratory disease processes, with 12 cases having HIV/TB co-infection.Keywords: Sudden; Unexplained and unexpected death; Post mortem; HIV; Mortuary; Pretoria; South Africa

scholarly journals Effect of Different Anti-Coagulants on the Accuracy of Glycated Haemoglobin Results

2019 ◽  
pp. 1-5
Author(s):  
Frederick Igila Allison ◽  
Duru Ugochukwu Stephen
Keyword(s):  
Blood Sample ◽  
Sampling Method ◽  
Chromatographic Method ◽  
Glycated Haemoglobin ◽  
Good Stability ◽  
Assay Method ◽  
Sample Collection ◽  
Mean Values ◽  
The Third ◽  
The Mean

Background: Most manufacturers of glycated haemoglobin kits advocate for the use of EDTA bottles for sample collection. Other manufacturers even when using the same glycated haemoglobin assay method, advocate for the use of any of these anticoagulant: EDTA, heparin and fluoride oxalate as any of these anticoagulants for sample collection. Aim: This study was therefore designed to evaluate the effect of different anticoagulants on the accuracy of glycated haemoglobin value using the same method. Methods: Thirty subjects were selected by purposive sampling method and 2ml of blood was collected from each subject into sodium heparin, EDTA and fluoride oxalate bottles and stored for three days at 4ºC. Fifteen subjects’ samples were analysed daily for the next two days then all the samples were analysed on the third day. All samples were analysed using the boronate affinity chromatographic method by Clover. Results: The mean of the values of glycated haemoglobin of samples for each anticoagulants were about the same for the first, second and third day. The differences in the mean values for each anticoagulant were not statistically significant, indicating fairly good stability. Conclusion: From this study, it could be concluded that blood sample in EDTA, fluoride oxalate and heparin bottles can be used for glycated haemoglobin estimation without affecting the accuracy of the result. These samples in these containers were found to be stable for at least three days.

scholarly journals Volumetric absorptive microsampling: its use in COVID-19 research and testing

Bioanalysis ◽  
2021 ◽  
Author(s):  
James Rudge ◽  
Stuart Kushon
Keyword(s):  
Clinical Trials ◽  
Antibody Response ◽  
Sample Collection ◽  
Independent Research ◽  
High Quality ◽  
Blood Samples ◽  
Viral Spread ◽  
Highly Effective ◽  
The Relationship ◽  
Volumetric Absorptive Microsampling

COVID-19 led to changes in the way blood samples are collected. As societies were isolated to control viral spread, access to facilities became limited. Remote sample collection with a volumetric microsampling approach, using Mitra® devices based on VAMS® technology, proved to be highly effective. It allowed people to collect high-quality samples at home and post them to a laboratory. This enabled scientists to conduct large serosurveillance studies, with results showing that seroprevalence of COVID-19 was higher than initially expected. Furthermore, remote microsampling studies by several institutions were conducted to measure the relationship between antigen levels and antibody response and duration. VAMS technology was also used in COVID-19 clinical trials. In summary, the independent research reviewed in this paper proved that VAMS is an effective sample collection alternative.

scholarly journals Conformation of the clinical diagnoses of some malnutrition diseases in local and Shammi goats in Baghdad province

2012 ◽  
Vol 36 (1) ◽  
pp. 8-14
Author(s):  
Al-Shawi A.F. S.
Keyword(s):  
Body Temperature ◽  
Normal Values ◽  
Mean Body Temperature ◽  
Local Breed ◽  
Blood Samples ◽  
Clinical Diagnoses ◽  
The Mean ◽  
Phosphorus And Potassium ◽  
Hb Concentration ◽  
The Relationship

The relationship between the clinical singes and some of the hematological and biochemical values in 126 cases of local breed and Shammi breed goats (from both sexes) diagnosed as cases of malnutrition from 230 goats examined .The clinical examination include (beside the general inspection and case history) body temperature, respiratory and pulse rates, mucus membranes skin and coat. Examination of the blood samples included RBCs count, Hb concentration, PCV % and values of cupper, magnesium, phosphorus, and potassium. The results showed that the mean body temperature of these goats was lower than in normal goats, while the respiratory and pulse rates were higher .Examination of blood revealed decrease in the means of RBCs counts ,Hb concentration and PCV % in the clinically diagnosed cases , and the decrease was more sever in local goats . However the results indicated lower values of cupper, magnesium, phosphorus, and potassium in comparison with normal values in goats. The ratio of cupper deficiency was the highest followed by phosphorus, magnesium and potassium and the ratio of malnutrition was high in Baghdad province. It was in Shammi breed higher than in local breed.

scholarly journals Conformation of the clinical diagnoses of some malnutrition diseases in local and Shammi goats in Baghdad province

2012 ◽  
Vol 36 (1) ◽  
pp. 8-14
Author(s):  
Al-Shawi A.F. S.
Keyword(s):  
Body Temperature ◽  
Normal Values ◽  
Mean Body Temperature ◽  
Local Breed ◽  
Blood Samples ◽  
Clinical Diagnoses ◽  
The Mean ◽  
Phosphorus And Potassium ◽  
Hb Concentration ◽  
The Relationship

The relationship between the clinical singes and some of the hematological and biochemical values in 126 cases of local breed and Shammi breed goats (from both sexes) diagnosed as cases of malnutrition from 230 goats examined .The clinical examination include (beside the general inspection and case history) body temperature, respiratory and pulse rates, mucus membranes skin and coat. Examination of the blood samples included RBCs count, Hb concentration, PCV % and values of cupper, magnesium, phosphorus, and potassium. The results showed that the mean body temperature of these goats was lower than in normal goats, while the respiratory and pulse rates were higher .Examination of blood revealed decrease in the means of RBCs counts ,Hb concentration and PCV % in the clinically diagnosed cases , and the decrease was more sever in local goats . However the results indicated lower values of cupper, magnesium, phosphorus, and potassium in comparison with normal values in goats. The ratio of cupper deficiency was the highest followed by phosphorus, magnesium and potassium and the ratio of malnutrition was high in Baghdad province. It was in Shammi breed higher than in local breed.

scholarly journals Investigation of Internet Addiction and Its Relationship with Sleep Quality and Depression among Dormitory Students of Larestan School of Health

2021 ◽  
Author(s):  
Bahram KOUHNAVARDT ◽  
Marjan ASDIAR ◽  
Marzieh HONARBAKHSH ◽  
Mohammad Reza ZARE ◽  
Zahra SHAHMOHAMMADI
Keyword(s):  
Sleep Quality ◽  
Internet Addiction ◽  
Analytical Study ◽  
Sampling Method ◽  
Statistical Tests ◽  
Poor Sleep ◽  
Poor Sleep Quality ◽  
Negative Effect ◽  
The Mean ◽  
The Relationship

Introduction: Internet addiction has a negative effect on mental health and leads to clinical discomfort and disorder. Therefore, this study was conducted to investigate the relationship between Internet addiction with sleep quality and depression in dormitory students. Methods: This study was a descriptive-analytical study. Since all students living in dormitories participated in this study (95 people), the sampling method was census. The study used three Young Internet addiction questionnaires, the Goldberg Depression Inventory, and the Petersburg Sleep Quality Questionnaire. For statistical analysis of data, descriptive statistics such as mean, standard deviation and also analytical statistical tests such as one-way analysis of variance with SPSS 22 software were used. Results: Among the students studied, 63 (66.3%) were normal in terms of Internet addiction, 31 (32.6%) were mildly addicted and 1 was severely addicted to the Internet. The study of depression among students showed that out of 95 people, 22 people have mild to moderate depression and 10 people have borderline depression. The mean score of students' sleep quality was 7.38 ± 2.88 and there was a significant relationship between it and Internet addiction (P <0.001) Discussion and Conclusion: Given the prevalence of Internet addiction among students and its significant association with depression and poor sleep quality, the necessary planning should be done to prevent this health problem.

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