Computer-Aided Three-Dimensional Reconstruction and Measurement of the Vestibular End-Organs

1988 ◽  
Vol 98 (3) ◽  
pp. 195-202 ◽  
Author(s):  
Akira Takagi ◽  
Isamu Sando ◽  
Akira Takagi ◽  
Isamu Sando
Keyword(s):  
Temporal Bone ◽  
Three Dimensional ◽  
Three Dimensions ◽  
Small Computer ◽  
Lateral Semicircular Canal ◽  
3 Dimensional ◽  
Dimensional Reconstruction ◽  
Vestibular Endorgans ◽  
Length And Area ◽  
Bone Structures

It is very valuable for temporal bone morphologists to be able to recognize temporal bone serial sections in three dimensions and to be able to measure temporal bone structures three-dimensionally. We can now do 3-dimensional reconstruction to visualize the structures of vestibular endorgans (utricular and saccular maculae) and measure these endorgans in space by means of a small computer system and software that we developed. As well as obtaining the dimensions—such as length and area—of the utricular and saccular maculae, we also found that (1) most of the utricular macula lies in one plane, which is the same as the plane of the lateral semicircular canal, (2) the saccular macula is shaped like part of a sphere, and (3) the angle between the two maculae is less than a right angle. Such knowledge is indispensable to the evaluation of the function of the utricular and saccular maculae.)

Computer-Aided Three-Dimensional Reconstruction: A Method of Measuring Temporal Bone Structures Including the Length of the Cochlea

1989 ◽  
Vol 98 (7) ◽  
pp. 515-522 ◽  
Author(s):  
Akira Takagi ◽  
Isamu Sando
Keyword(s):  
Temporal Bone ◽  
Three Dimensional ◽  
Computer Software ◽  
Three Dimensions ◽  
Reconstruction Method ◽  
Two Dimensional ◽  
Three Dimensional Reconstruction ◽  
Computer Aided ◽  
Dimensional Reconstruction ◽  
Bone Structures

To visualize the three-dimensional (3-D) shapes of structures in the temporal bone from histologic sections, we developed computer software to create computer-generated 3-D images from two-dimensional (2-D) materials. Using those images, we have been able to measure these structures in three dimensions. This means that quantitative 3-D measurements can be performed regardless of the angle at which the specimen was cut. This report describes this method for 3-D reconstruction and measurement and discusses its implications. For instance, in addition to showing the 3-D shape of the cochlea from different angles, we have been able to measure the length of the cochlea and have shown that measurements made by use of the conventional 2-D graphic reconstruction method can be distorted considerably by variations in cutting angle of the specimen.

Three-Dimensional Reconstruction Using Stereo Pairs from Serial Thick Sections

1977 ◽  
Vol 35 ◽  
pp. 562-563
Author(s):  
Robert Glaeser ◽  
Thomas Bauer ◽  
David Grano
Keyword(s):  
Three Dimensional ◽  
Dimensional Structure ◽  
Serial Sections ◽  
Thin Sections ◽  
3 Dimensional ◽  
Transmission Electron ◽  
Freeze Dried ◽  
Dimensional Reconstruction ◽  
Stereo Imagery ◽  
Whole Mounts

In transmission electron microscopy, the 3-dimensional structure of an object is usually obtained in one of two ways. For objects which can be included in one specimen, as for example with elements included in freeze- dried whole mounts and examined with a high voltage microscope, stereo pairs can be obtained which exhibit the 3-D structure of the element. For objects which can not be included in one specimen, the 3-D shape is obtained by reconstruction from serial sections. However, without stereo imagery, only detail which remains constant within the thickness of the section can be used in the reconstruction; consequently, the choice is between a low resolution reconstruction using a few thick sections and a better resolution reconstruction using many thin sections, generally a tedious chore. This paper describes an approach to 3-D reconstruction which uses stereo images of serial thick sections to reconstruct an object including detail which changes within the depth of an individual thick section.

Surgical considerations during cochlear implantation: the utility of temporal bone computed tomography

2021 ◽  
pp. 1-8
Author(s):  
Beomcho Jun ◽  
Sunwha Song
Keyword(s):  
Computed Tomography ◽  
Temporal Bone ◽  
Cochlear Implantation ◽  
Round Window ◽  
Three Dimensional ◽  
Electrode Placement ◽  
Posterior Tympanotomy ◽  
Electrode Insertion ◽  
Window Approach ◽  
Bone Structures

Abstract Objective This paper describes the construction of portals for electrode placement during cochlear implantation and emphasises the utility of pre-operative temporal bone three-dimensional computed tomography. Methods Temporal bone three-dimensional computed tomography was used to plan portal creation for electrode insertion. Results Pre-operative temporal bone three-dimensional computed tomography can be used to determine the orientation of temporal bone structures, which is important for mastoidectomy, posterior tympanotomy and cochleostomy, and when using the round window approach. Conclusion It is essential to create appropriate portals (from the mastoid cortex to the cochlea) in a step-by-step manner, to ensure the safe insertion of electrodes into the scala tympani. Pre-operative three-dimensional temporal bone computed tomography is invaluable in this respect.

Three-dimensional reconstruction of metabolic data from quantitative autoradiography of rat brain

1984 ◽  
Vol 247 (3) ◽  
pp. E412-E419 ◽  
Author(s):  
L. S. Hibbard ◽  
R. A. Hawkins
Keyword(s):  
High Speed ◽  
Three Dimensional ◽  
Image Data ◽  
Brain Structures ◽  
Three Dimensions ◽  
Quantitative Autoradiography ◽  
Alignment Algorithm ◽  
Computer Methods ◽  
Mathematical Properties ◽  
Dimensional Reconstruction

Quantitative autoradiography is a powerful method for studying brain function by the determination of blood flow, glucose utilization, or transport of essential nutrients. Autoradiographic images contain vast amounts of potentially useful information, but conventional analyses can practically sample the data at only a small number of points arbitrarily chosen by the experimenter to represent discrete brain structures. To use image data more fully, computer methods for its acquisition, storage, quantitative analysis, and display are required. We have developed a system of computer programs that performs these tasks and has the following features: 1) editing and analysis of single images using interactive graphics, 2) an automatic image alignment algorithm that places images in register with one another using only the mathematical properties of the images themselves, 3) the calculation of mean images from equivalent images in different experimental serial image sets, 4) the calculation of difference images (e.g., experiment-minus-control) with the option to display only differences estimated to be statistically significant, and 5) the display of serial image metabolic maps reconstructed in three dimensions using a high-speed computer graphics system.

scholarly journals Hematopoiesis in 3 dimensions: human and murine bone marrow architecture visualized by confocal microscopy

Blood ◽  
2010 ◽  
Vol 116 (15) ◽  
pp. e41-e55 ◽  
Author(s):  
Tomoiku Takaku ◽  
Daniela Malide ◽  
Jichun Chen ◽  
Rodrigo T. Calado ◽  
Sachiko Kajigaya ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Three Dimensional ◽  
Cell Types ◽  
Bone Marrow Tissue ◽  
Murine Bone Marrow ◽  
3 Dimensional ◽  
Vascular Structures ◽  
Bone Structures ◽  
Surrounding Bone

AbstractIn many animals, blood cell production occurs in the bone marrow. Hematopoiesis is complex, requiring self-renewing and pluripotent stem cells, differentiated progenitor and precursor cells, and supportive stroma, adipose tissue, vascular structures, and extracellular matrix. Although imaging is a vital tool in hematology research, the 3-dimensional architecture of the bone marrow tissue in situ remains largely uncharacterized. The major hindrance to imaging the intact marrow is the surrounding bone structures are almost impossible to cut/image through. We have overcome these obstacles and describe a method whereby whole-mounts of bone marrow tissue were immunostained and imaged in 3 dimensions by confocal fluorescence and reflection microscopy. We have successfully mapped by multicolor immunofluorescence the localization pattern of as many as 4 cell features simultaneously over large tiled views and to depths of approximately 150 μm. Three-dimensional images can be assessed qualitatively and quantitatively to appreciate the distribution of cell types and their interrelationships, with minimal perturbations of the tissue. We demonstrate its application to normal mouse and human marrow, to murine models of marrow failure, and to patients with aplastic anemia, myeloid, and lymphoid cell malignancies. The technique should be generally adaptable for basic laboratory investigation and for clinical diagnosis of hematologic diseases.

Three-Dimensional Computer Reconstruction of a Temporal Bone

1989 ◽  
Vol 101 (5) ◽  
pp. 522-526 ◽  
Author(s):  
Charles Lutz ◽  
Akira Takagi ◽  
Ivo P. Janecka ◽  
Isamu Sando
Keyword(s):  
Temporal Bone ◽  
Internal Carotid ◽  
Computer Graphic ◽  
Three Dimensional ◽  
Computer Assisted ◽  
Computer Reconstruction ◽  
Temporal Bone Anatomy ◽  
Bone Structures ◽  
Graphic Software ◽  
Computer Graphics System

The complexities of the temporal bone and the critical inter-relationships among its key structures can be simplified with three-dimensional computer-assisted reconstruction. Knowledge of the topography of these structures and their mutual relationships in essential in any surgical approach to the temporal bone. Sixty sagittal histologic sections of a normal left temporal bone were examined. Each section, 30 μm in thickness, was optically enlarged. Segments representing the facial nerve, internal carotid artery, and inner ear structures from individual slides were traced and data were entered into a computer. A personal computer was used for data processing and analysis. Graphic software developed in our laboratory generated images with x-y-z coordinates that could be rotated In any plane. The high resolution of the computer graphics system, combined with the precision of histologic sections, permitted study of the critical three-dimensional anatomic relationships among essential intratemporal bone structures. The capability of reproducing individual and joint images of the intratemporal bone structures and viewing them from all surgical angles gives skull base and otologic surgeons Important topographic guidance. Accurate spatial measurements of temporal bone anatomy are now possible with the application of computer graphic technology.

Three-dimensional reconstruction of the chromatin bodies in the nuclei of mature erythrocytes from the newt Triturus cristatus: the number of nuclear envelope-attachment sites

1979 ◽  
Vol 35 (1) ◽  
pp. 59-66
Author(s):  
A.B. Murray ◽  
H.G. Davies
Keyword(s):  
Nuclear Envelope ◽  
Three Dimensional ◽  
Interphase Nuclei ◽  
Interphase Chromosome ◽  
Triturus Cristatus ◽  
3 Dimensional ◽  
Attachment Sites ◽  
Chromatin Body ◽  
Dimensional Reconstruction ◽  
Discrete Site

The arrangement of the chromatin bodies in the interphase nuclei of 6 erythrocytes has been investigated by means of 3-dimensional reconstruction from electron micrographs of serial sections. When the borders of chromatin bodies are marked on the surface of each model, discrete areas of chromatin in contact with the nuclear envelope are revealed. The number of these areas in approximately equal to the number of chromosomes in the diploid set. The data suggest that each chromatin body corresponds to a condensed interphase chromosome and that each chromosome is attached to one discrete site on the nuclear envelope. The data are insufficient to show whether or not the condensed chromosomes are arranged in any orderly pattern in these nuclei.

Three-Dimensional Reconstruction of the Topographical Cerebral Surface Anatomy for Presurgical Planning With Free OsiriX Software

2014 ◽  
Vol 10 (3) ◽  
pp. 426-435 ◽  
Author(s):  
Mehmet V. Harput ◽  
Pablo Gonzalez-Lopez ◽  
Uğur Türe
Keyword(s):  
Three Dimensional ◽  
Computer Software ◽  
Tumor Location ◽  
Magnetic Resonance Images ◽  
Indocyanine Green Videoangiography ◽  
3 Dimensional ◽  
Surface Anatomy ◽  
Dimensional Reconstruction ◽  
Surgical Field ◽  
High Resolution Ultrasonography

Abstract BACKGROUND: During surgery for intrinsic brain lesions, it is important to distinguish the pathological gyrus from the surrounding normal sulci and gyri. This task is usually tedious because of the pia-arachnoid membranes with their arterial and venous complexes that obscure the underlying anatomy. Moreover, most tumors grow in the white matter without initially distorting the cortical anatomy, making their direct visualization more difficult. OBJECTIVE: To create and evaluate a simple and free surgical planning tool to simulate the anatomy of the surgical field with and without vessels. METHODS: We used free computer software (OsiriX Medical Imaging Software) that allowed us to create 3-dimensional reconstructions of the cerebral surface with and without cortical vessels. These reconstructions made use of magnetic resonance images from 51 patients with neocortical supratentorial lesions operated on over a period of 21 months (June 2011 to February 2013). The 3-dimensional (3-D) anatomic images were compared with the true surgical view to evaluate their accuracy. In all patients, the landmarks determined by 3-D reconstruction were cross-checked during surgery with high-resolution ultrasonography; in select cases, they were also checked with indocyanine green videoangiography. RESULTS: The reconstructed neurovascular structures were confirmed intraoperatively in all patients. We found this technique to be extremely useful in achieving pure lesionectomy, as it defines tumor's borders precisely. CONCLUSION: A 3-D reconstruction of the cortical surface can be easily created with free OsiriX software. This technique helps the surgeon perfect the mentally created 3-D picture of the tumor location to carry out cleaner, safer surgeries.

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