Testing Wound-healing Activity in T15 Fibroblast Cultures: A Morphometric Analysis

2000 ◽  
Vol 28 (1) ◽  
pp. 41-51 ◽  
Author(s):  
Seval Korkmaz ◽  
Hülya Zeytinoglu ◽  
Melih Zeytinoglu ◽  
Süleyman Aydin ◽  
Yusuf Öztürk ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Cultures ◽  
Centella Asiatica ◽  
Fibroblast Cell ◽  
Mechanisms Of Action ◽  
Cell Staining ◽  
Morphometric Analyses ◽  
Pharmacological Screening ◽  
Wound Healing Activity ◽  
Intracellular Collagen

The purpose was to evaluate the use of mouse T15 fibroblast cell cultures for the investigation of wound-healing activity. In order to investigate their mechanisms of action, the effects of drugs with wound-healing activities were compared by using morphometric analyses by microscopy after cell staining. A number of parameters were used to evaluate the effects of titrated extracts from Centella asiatica and dexpanthenol (drugs that have been used in medical practice for their wound-healing activities) on cultured mouse T15 fibroblasts. These parameters were: the total number of cells; the number of T15 cells in mitosis; the percentages of fusiform, polygonal, round and vacuole-containing cells; and the number of intracellular collagen granules. The results indicate that these two drugs exhibit wound-healing activities by activating fibroblast cells, and have cytoprotective effects, although their mechanisms of action on mouse T15 fibroblasts were different. On the basis of our findings, mouse T15 fibroblast cell cultures seem to be useful for the pharmacological screening of compounds with wound-healing activity.

scholarly journals Wound Healing Activity of α-Pinene and α-Phellandrene

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2488
Author(s):  
Judith Salas-Oropeza ◽  
Manuel Jimenez-Estrada ◽  
Armando Perez-Torres ◽  
Andres Eliu Castell-Rodriguez ◽  
Rodolfo Becerril-Millan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Folk Medicine ◽  
Fibroblast Cell ◽  
In Vitro Tests ◽  
Low Concentrations ◽  
In Vivo Tests ◽  
Wound Healing Activity

Bursera morelensis is used in Mexican folk medicine to treat wounds on the skin. Recently, it was shown that the essential oil (EO) of B. morelensis has wound healing activity, accelerating cutaneous wound closure and generating scars with good tensile strength. α-pinene (PIN) and α-phellandrene (FEL) are terpenes that have been found in this EO, and it has been shown in different studies that both have anti-inflammatory activity. The aim of this study was to determine the wound healing activity of these two terpenes. The results of in vitro tests demonstrate that PIN and FEL are not cytotoxic at low concentrations and that they do not stimulate fibroblast cell proliferation. In vivo tests showed that the terpenes produce stress-resistant scars and accelerate wound contraction, due to collagen deposition from the early stages, in wounds treated with both terpenes. Therefore, we conclude that both α-pinene and α-phellandrene promote the healing process; this confirms the healing activity of the EO of B. morelensis, since having these terpenes as part of its chemical composition explains part of its demonstrated activity.

Design, Network Analysis and In Silico Modeling of Biologically Significant 4-(substituted benzyl)-2-Amino-6-HydroxyPyrimidine-5-Carboxamide Nanoparticles

Drug Research ◽  
2017 ◽  
Vol 67 (05) ◽  
pp. 289-301
Author(s):  
Theivendren Panneerselvam ◽  
Vellaichamy Sivakumar ◽  
Subramanian Arumugam ◽  
Kunjiappan Selvaraj ◽  
Murugan Indhumathy
Keyword(s):  
Wound Healing ◽  
Pain Management ◽  
Network Analysis ◽  
In Silico ◽  
Synthesis Of Nanoparticles ◽  
Wound Model ◽  
In Silico Modeling ◽  
Human Proteins ◽  
Pharmacological Screening ◽  
Wound Healing Activity

Abstract The synthesized 4-(4-hydroxy benzyl)-2-amino-6-hydroxy pyrimidine-5-carboxamide was chosen to perform in silico modeling with identified drug target AGT, TNF, F2 and BCL2L1. The identified human proteins are vital in the pain management and also an important target for the study of wound healing activity. The enzymes were identified by using BioGRID, string database and network analysis through Cytoscape software. The wound healing activity was evaluated by excision wound model. The observed results revealed that, the pyrimidine nanoparticles showed significant wound healing activity compared to standard and synthesized compound. The detailed synthesis of nanoparticles formulation spectral analysis and pharmacological screening data’s were reported. The revealed reports of synthesized analogues and formulated nanoparticles will generate a very good impact to the chemists and research scholars for further investigations in wound healing and pain management.

In vitro and in vivo wound healing activity of asiaticoside isolated from Centella asiatica

1999 ◽  
Vol 65 (1) ◽  
pp. 1-11 ◽  
Author(s):  
A. Shukla ◽  
A.M. Rasik ◽  
G.K. Jain ◽  
R. Shankar ◽  
D.K. Kulshrestha ◽  
...  
Keyword(s):  
Wound Healing ◽  
Centella Asiatica ◽  
Wound Healing Activity

scholarly journals Comparative Study of Various Pharmacological Screening of Argyreia Speciosa Sweet. In Relation with Ayurvedic Documented Literature

1970 ◽  
Vol 7 (5) ◽  
pp. 37-41
Author(s):  
K P Unadkat ◽  
D K Jani ◽  
R C Pandey
Keyword(s):  
Wound Healing ◽  
Comparative Study ◽  
Traditional Healers ◽  
Health It ◽  
Anti Cancer ◽  
Argyreia Nervosa ◽  
Pharmacological Screening ◽  
Anti Fungal ◽  
Pharmacological Testing ◽  
Wound Healing Activity

Vruddhadaru is one of the important herbs in Ayurveda. It has been used in Ayurvedic traditional literature for various diseases and improves the health. It is also used as folklore medicine. Out of its entire therapeutic spectrum, the herb is famous for its wound healing activity for longer time. The Latin name of Vruddhadaru is Argyreia speciosa(Syn.-Argyreia nervosa; Family-Convolvulaceae).Argyreia speciosa is commonly known as Vruddhadaru in Sanskrit, Vidhara in Hindi, Elephant creeper in English and Samudrashosha in Gujarati. It has many synonyms like Aavegi, Chhagalantri, Vrushyagandhika etc.  It has been described in Dhanvantari Nighantu, Madanpala Nighantu, Kaiyadeva Nighantu, Raj Nighantu, Bhavaprakasha Nighantu, Nighantu Adarsh etc. This shows the importance of the drug. It is usually appreciated for its aesthetic merit. Therapeutically proven activities of this plant are – analgesic & anti-inflammatory, anti-cancer, anti-convulsant, anti-diarrheal, anti-fungal, anti-microbial, anti-obesity, antipyretic, anti-stress, anti-ulcer, anti-viral, aphrodisiac, CNS effect, hepatoprotective, hypoglycaemic, immunomodulatory, nootropic, wound healing activity etc. It is very interesting to observe that most of the pharmacological screened activities are documented in Ayurveda by various authors but in the language of Ayurveda. It shows that the former scientists (Ayurveda Philosophers) are extreme aware about the activity of the herb and was in practice as an Ayurveda medicine. The conventional evidences about the activity are the proof that there must be a scientific technique with the traditional healers to document the properties of the herb. The present review explains the correlation of the same activity noted by two different streams. This review helps to design futuristic pathway for pharmacological testing of herbs.     Keywords: Vruddhadaaru, Argyreia speciosa, pharmacological screening, Ayurveda

scholarly journals Wound Healing Activity and Mechanisms of Action of an Antibacterial Protein from the Venom of the Eastern Diamondback Rattlesnake (Crotalus adamanteus)

PLoS ONE ◽  
2014 ◽  
Vol 9 (2) ◽  
pp. e80199 ◽  
Author(s):  
Ramar Perumal Samy ◽  
Matheswaran Kandasamy ◽  
Ponnampalam Gopalakrishnakone ◽  
Bradley G. Stiles ◽  
Edward G. Rowan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mechanisms Of Action ◽  
Antibacterial Protein ◽  
Diamondback Rattlesnake ◽  
Wound Healing Activity

The diabetic wound healing effect of a two-herb formula and its mechanisms of action

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
CBS Lau ◽  
VKM Lau ◽  
CL Liu ◽  
PKK Lai ◽  
JCW Tam ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mechanisms Of Action ◽  
Diabetic Wound ◽  
Healing Effect ◽  
Diabetic Wound Healing ◽  
Wound Healing Effect

Wound Healing Activity of Ten Indigenous Plants in Incision and Excision Wound Models in Albino Rats

2012 ◽  
Vol 2 (9) ◽  
pp. 56-58
Author(s):  
Vasanthalaxmi K Vasanthalaxmi K ◽  
◽  
Saraswathi Udupa ◽  
Laximinarayana Udupa
Keyword(s):  
Wound Healing ◽  
Albino Rats ◽  
Indigenous Plants ◽  
Excision Wound ◽  
Wound Healing Activity

Comparison of the ficus for the wound healing activity in various species

2020 ◽  
Vol 10 (4) ◽  
pp. 67-70
Author(s):  
Mothilal K ◽  
Akila CR ◽  
Mahender K ◽  
Chaitanya Kumar K ◽  
Ravi D
Keyword(s):  
Wound Healing ◽  
Wound Closure ◽  
Subcutaneous Tissue ◽  
Significant Activity ◽  
Herbal Extracts ◽  
Standard Drug ◽  
Excision Wound ◽  
Injuries And Wounds ◽  
Skin Conditions ◽  
Wound Healing Activity

Injuries and wounds are any sorts of damage to the skin or subcutaneous tissue. Usually, any wounds of such sorts are self-healed. Sometimes, there may be a delay in healing, and that delay is caused due to the functional delays in various processes of wound healing. All the Ficus plants show similar activities like the antioxidant, anti-inflammatory and wound healing properties 7including skin conditions like ulcers and rheumatism. The anthelmintic property and immunomodulatory are also seen. The herbal extracts of the same family of Ficus in different plants were investigated for the wound healing activity in the excision wound method, and the extracts showed significant activity compared to the drug. All the extracts showed a better healing ability, but the extract of FBO-100 showed the highest activity followed by FMO followed by FHO and finally the FRO. Overall, the activity of the extract ointment was comparable and was significant compared to the standard drug ointment. The wound closure of the extract ointment treated groups were better and were completed in 12 days, and the activity was more than 96%. The herbal extracts of the same family of Ficus in different plants were investigated for the wound healing activity in the excision wound method, and the extracts showed significant activity compared to the drug. The plants of microcarpa, benghalensis, religiosa and hispida are compared for the activity, and the order showed for the activity was FBO>FMO>FHO>FRO.

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