Design, Network Analysis and In Silico Modeling of Biologically Significant 4-(substituted benzyl)-2-Amino-6-HydroxyPyrimidine-5-Carboxamide Nanoparticles

Drug Research ◽  
2017 ◽  
Vol 67 (05) ◽  
pp. 289-301
Author(s):  
Theivendren Panneerselvam ◽  
Vellaichamy Sivakumar ◽  
Subramanian Arumugam ◽  
Kunjiappan Selvaraj ◽  
Murugan Indhumathy
Keyword(s):  
Wound Healing ◽  
Pain Management ◽  
Network Analysis ◽  
In Silico ◽  
Synthesis Of Nanoparticles ◽  
Wound Model ◽  
In Silico Modeling ◽  
Human Proteins ◽  
Pharmacological Screening ◽  
Wound Healing Activity

Abstract The synthesized 4-(4-hydroxy benzyl)-2-amino-6-hydroxy pyrimidine-5-carboxamide was chosen to perform in silico modeling with identified drug target AGT, TNF, F2 and BCL2L1. The identified human proteins are vital in the pain management and also an important target for the study of wound healing activity. The enzymes were identified by using BioGRID, string database and network analysis through Cytoscape software. The wound healing activity was evaluated by excision wound model. The observed results revealed that, the pyrimidine nanoparticles showed significant wound healing activity compared to standard and synthesized compound. The detailed synthesis of nanoparticles formulation spectral analysis and pharmacological screening data’s were reported. The revealed reports of synthesized analogues and formulated nanoparticles will generate a very good impact to the chemists and research scholars for further investigations in wound healing and pain management.

scholarly journals EVALUATION OF WOUND HEALING ACTIVITY OF METHANOLIC EXTRACT OF SMILAX WIGHTII (A. DC.) IN WISTAR ALBINO RATS

2014 ◽  
Vol 1 (1) ◽  
pp. 73-77
Author(s):  
Uma Maheswari P ◽  
Shalimol A ◽  
Arumugasamy A ◽  
Udhaya Sankar M.R
Keyword(s):  
Wound Healing ◽  
Dry Weight ◽  
Wound Contraction ◽  
Albino Rats ◽  
Standard Drug ◽  
Excision Wound ◽  
Wound Model ◽  
Wistar Albino Rats ◽  
Incision Wound ◽  
Wound Healing Activity

The present experimental study was designed to evaluate the wound healing activity of methanolic extract of Smilax wightii A. DC. on incision and excision wound models in Wistar albino rats. The parameters studied were wound breaking strength, wound contraction area, epithelialization period, granulation tissuewet, dry weight and hydroxyproline content in incision wound model, percentage of wound contraction and period of epithelialization in excision wound model. The rats were administered topically with 100mg/kg b.wt. (low dosage), 200mg/kg b.wt. (moderate dosage) and 500mg/kg b.wt. (high dosage) of methanolicextract of Smilax wightii (MESW). The activity of the extract treated groups were compared with that of the control 1% Spirit. Framycetin sulphate 0.2% w/w was used as the standard drug. In incision wound model, there was a significant increase in the wound breaking strength in all the experimental groups treated withMESW than that of the control. Similarly, significant (P<0.001) decrease in wound contraction area and period of epithelialization were also observed in the test group animals treated with MESW and the standard drug treated groups when compared to that of the control. , a significant increase was observed in granulation tissue wet and dry weight and hydroxyproline content in the test groups treated with MESW compared to the control. In exicision wound model, there was a significant increase (P<0.01) in the percentage of wound contraction and decrease in period of epithelialization in the experimental groups treated with 200mg/kgb.wt. (moderate dosage) and 500mg/kgb.wt (high dosage) of MESW. The extract treated groups showed significant improvement in all the wound healing parameters of incision, and excision wound models.

scholarly journals Evaluation of wound healing activity of alpha mangostin ointment in rats

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 1080-1087
Author(s):  
Sasikala Chinnappan ◽  
Venkatalakshmi Ranganathan ◽  
Jithendra Panneerselvam ◽  
Barani Karikalan ◽  
Thivashini vasanthan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Care ◽  
Inflammatory Cells ◽  
Treated Group ◽  
Wound Model ◽  
Wound Size ◽  
Group I ◽  
Significant Difference ◽  
Histopathological Studies ◽  
Wound Healing Activity

This study was aimed to evaluate the wound healing effects of alpha mangostin ointment using excision wound model. Twenty rats were divided into four groups of five rats each; group I was treated with ointment base (control), whereas group II, group III and group IV were treated with 10% (w/w) povidone-iodine (standard), alpha mangostin 1% (w/w), and alpha mangostin 2 % (w/w) respectively for wound healing evaluations. The size of the wound area was measured, and the reduction in the wound size was calculated, and the tissues examined histologically. The significant difference in the wound size reduction between the control and treated group (p<0.05) was observed in wound healing activity. Histopathological studies showed a lesser number of chronic inflammatory cells, lesser oedema and increased collagenisation in the test than control. This study showed that the alpha mangostin topical ointment could be a promising candidate for the betterment of wound care.

EVALUATION OF EXCISIONAL AND INCISIONAL WOUND HEALING ACTIVITY OF ELECTROHOMEOPATHIC DRUG (SPAGYRIC ESSENCE) GREEN ELECTRICITY IN RATS

2020 ◽  
pp. 72-75
Author(s):  
PRASANT KUMAR SABAT ◽  
SWETA PRIYADARSINI PRADHAN ◽  
RANDEEP PATRO
Keyword(s):  
Wound Healing ◽  
Tensile Strength ◽  
Wound Contraction ◽  
Control Group ◽  
Albino Rats ◽  
Green Electricity ◽  
Wound Model ◽  
Wound Protection ◽  
Incision Wound ◽  
Wound Healing Activity

Objective: The current research is an attempt to evaluate the wound healing activity of Electrohomeopathic drug Green electricity (GE) in Wistar rats. No data have been reported till date on the wound healing effect of GE. Hence the present research focuses on the scientific investigation of the wound healing activity of GE in rats. Methods: Excision and incision wounds were inflicted upon albino rats. In the excision wound model, the wound contraction, Scar residue, area and time of complete epithelization were measured subsequently on day 0, 3,6,9,12,15, 18 and 21 after inflicting the wound. Wound contraction was calculated as a percentage change in the initial wound size. In the incision wound model, the tensile strength was measured on the 10th post wounding day. Results: It was noted that the effect produced by the GE Showed significant wound healing (p<0.01) in all wound models when compared to the control group. In the case of the excision model, GE statistically increases the wound contraction rate and in the case of the incision model GE showed high tensile strength as compared to the control group. In both cases the epithelization period was significantly decreased as compared to control. Almost 100% wound protection was achieved by GE in 21 d as compared to control. Conclusion: GE (ointment) showed significant wound healing activity (p<0.01) in both the excised wound model and incision wound model in rats.

scholarly journals Wound Healing Activity of Crassocephalum crepidioides (Benth.) S. Moore. Leaf Hydroethanolic Extract

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Nguyen Minh Can ◽  
Dang Thi Phuong Thao
Keyword(s):  
Wound Healing ◽  
Fold Increase ◽  
Inflammatory Cells ◽  
Treated Group ◽  
Pcr Analysis ◽  
Wound Model ◽  
Vehicle Treatment ◽  
Hydroethanolic Extract ◽  
Anti Inflammation ◽  
Wound Healing Activity

Crassocephalum crepidioides (Benth.) S. Moore. has been used to treat small wounds by minority people in Lam Dong, Vietnam. However, there has been no scientific evidences about its wound healing activity. This study is aimed at evaluating the wound healing activity of Crassocephalum crepidioides hydroethanolic extract via its antioxidant and anti-inflammation activities and healing capability on a mouse excision wound model. Crassocephalum crepidioides hydroethanolic extract (CCLE) at a dose of 50 mg/kg/day reduced the wound closure time about 3.5 days, compared to vehicle treatment. The granulation tissue on day 7 after surgery from the treated group showed a 2.8-fold decrease in the density of inflammatory cells, 1.9-fold increase in the fibroblast density, and a higher number of blood vessels. Real-time PCR analysis indicated that the mRNA expression level of NF-κB1 and TNF-α mRNA in CCLE-treated wounds decreased by 4.6 and 3.3 times, respectively, while TGF-β1 and VEGF were found to increase by 3.3 and 2.4 times, respectively. Our experimental data provided proofs of Crassocephalum crepidioides leaf wound healing activity due to its antioxidant, anti-inflammation, fibroblast proliferation, wound contraction, and angiogenesis effects.

scholarly journals Comparative evaluation of wound healing activity of tilvadi ghrita and durva ghrita on diabetic wound model in rats

2019 ◽  
Vol 10 (4) ◽  
pp. 3376-3384
Author(s):  
Shakila Shabbeer Shaikh ◽  
Krishna Murthy ◽  
Rajkumar V. Shete ◽  
Rahul S. Solunke
Keyword(s):  
Wound Healing ◽  
Skin Irritation ◽  
Chemical Properties ◽  
Diabetic Control ◽  
Comparative Investigation ◽  
Diabetic Wound ◽  
Control Groups ◽  
Skin Reactions ◽  
Wound Model ◽  
Wound Healing Activity

Previous studies proved the wound healing potential of Tilvadi Ghrita (TG) and Durva Ghrita (DG) on normal wounds, so this study was conducted to investigate wound healing activity of TG and DG on diabetic wound model in rats. Forty-eight adult female Wistar rats in eight groups were used. Alloxan was used to induce diabetes in diabetic groups. Excision wound was made on the dorsal shaved surface of rats, and then Framycetin sulfate cream (FSC) 10%, TG and DG were applied in control and treatment groups. While in normal control and diabetic control groups, no medication was done. Wound photographs were taken on alternative days to evaluate wound healing. Percentage wound contraction, the effect on wound size, and epithelization period parameters were studied. Ghritas were investigated for their preliminary phytochemicals, Physico-chemical properties, skin irritation test, and wound healing activity in normal and diabetic rats. Data were expressed as mean ± SEM and analyzed using Two-way ANOVA followed by Bonferroni’s multiple range test. Studies revealed that TG and DG do not cause any harmful skin reactions, so both ghritas were found to be safe for topical application. Both formulations showed better and fast healing as compared to untreated normal and diabetic control groups. After comparative investigation, it was observed that DG possesses higher wound healing potential in normal and diabetic groups with comparison to TG. Wound healing action in normal and diabetic wounds, it endowed due to the presence of different phytoconstituents reported in the literature and proved to be beneficial in the management of wounds.

scholarly journals Wound healing activity of the flowers of Lilium candidum L. in burn wound model in rats

2020 ◽  
Vol 1 (73) ◽  
pp. 109-118
Author(s):  
Saeideh Momtaz ◽  
Mahshid Dibaj ◽  
Alireza Abdollahi ◽  
Gholamreza Amin ◽  
Roodabeh Bahramsoltani ◽  
...  
Keyword(s):  
Wound Healing ◽  
Burn Wound ◽  
Wound Model ◽  
Wound Healing Activity

Wound healing activity of Terminalia bellerica Roxb. and gallic acid in experimentally induced diabetic animals

2019 ◽  
Vol 17 (2) ◽  
Author(s):  
Manish Pal Singh ◽  
Avneet Gupta ◽  
Siddhraj Singh Sisodia
Keyword(s):  
Wound Healing ◽  
Gallic Acid ◽  
Dead Space ◽  
Diabetic Rats ◽  
Fruit Extract ◽  
Standard Drug ◽  
Terminalia Bellerica ◽  
Wound Model ◽  
Wound Healing Activity ◽  
Experimentally Induced

AbstractBackgroundDiabetic wound are the major problem of society because of its delay healing of wound in hyperglycemia patient. The target of this study need to know the gallic acid compelling synergistically wound healing in diabetic animals as it is available in fruit extract or is more effective in given pure form in diabetic animals.MethodsThe wound healing effects of fruit extract Terminalia bellerica Roxb. and gallic acid were evaluated in experimentally induced hyperglycemic animals. In this investigation, excision and dead space wound models were used. The contraction wound %, re-epithelialization days, area of scar, histopathological examination of tissue, hydroxyproline level and weight of tissue granuloma were evaluated in this study.ResultsTerminalia bellerica fruit extract (400 mg/Kg) and gallic acid (200 mg/Kg) have been ingested by orally in streptozotocin induced diabetic rats. They were founded significantly (p<0.05) improved percentage wound contraction and decreased the size of scar area and days of re-epithelialization in excision wound model. Also, they were improved the level of hydroxyproline level in diabetic rats as compared to diabetic control group in dead space wound model. Standard drug vitamin C was also founded significantly improved wound healing activity in rats. These results suggested that treated groups accelerated wound healing activity in diabetic rat.ConclusionsThe observations and results obtained in the present study indicated the wound healing efficacy of fruit ethanolic extract and gallic acid in diabetic animals. Yet, the wound healing effect was increasingly reported with gallic acid.

WOUND HEALING ACTIVITY OF THE LEAVES OF WRIGHTIA TINCTORIA (ROXB) R. BR

INDIAN DRUGS ◽  
2012 ◽  
Vol 49 (03) ◽  
pp. 40-46
Author(s):  
M. C. Divakar ◽  
◽  
S Lakshmi Devi
Keyword(s):  
Wound Healing ◽  
Tensile Strength ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Excision Wound ◽  
Wound Model ◽  
Methanolic Extracts ◽  
Wrightia Tinctoria ◽  
Incision Wound ◽  
Wound Healing Activity

The study was aimed to evaluate wound healing activity of 70% ethanolic and methanolic extracts of leaves of Wrightia tinctoria (Roxb) R. Br (Apocynaceae) using incision and excision wound models on Wistar rats. Wound contraction and period of epithelization were assessed in excision wound model whereas wound tensile strength was determined in case of incision wound model. 4% Wrightia tinctoria methanolic leaf extract phytosome exhibited significant wound healing potential when compared with standard 0.2% nitrofurazone ointment.

Evaluation of Wound Healing Activity of Topical Phenytoin in an Incision Wound Model in Rats

2016 ◽  
Vol 3 (2) ◽  
pp. 75
Author(s):  
Sarita Mulkalwar ◽  
Lopamudra Behera ◽  
Pravin Golande ◽  
Aadil Shah
Keyword(s):  
Wound Healing ◽  
Wound Model ◽  
Incision Wound ◽  
Wound Healing Activity
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