Herpes Simplex Virus and Human Cancer. III. Search for Relationship of Herpes Simplex Antibodies and Cervical Dysplasia and Labial Neoplasia

1983 ◽  
Vol 69 (2) ◽  
pp. 83-87 ◽  
Author(s):  
Antonella Rotola ◽  
Giuseppe Gerna ◽  
Dario Di Luca ◽  
Anna Rosa Virgili ◽  
Roberto Manservigi ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Antibody Titer ◽  
Human Cancer ◽  
Cervical Dysplasia ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Antigens ◽  
Simplex Virus ◽  
Relationship Of ◽  
Hsv 1

We employed the enzyme-linked immunosorbent assay (ELISA), indirect hemagglutination (IHA), and complement fixation (CF) methods to measure antibody titer to herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) in patients affected by labial tumors or cervical dysplasias. No relationship of antibody titer to HSV-1 and labial tumors was detected by any of the three methods. Association between antibody titer to HSV-2 and cervical dysplasias was revealed by IHA (p < 0.05) and ELISA (p < 0.001); CF tests were negative. Moreover, we assayed for HSV-specific antigens in cell cultures derived from labial tumors and cervical dysplasias. In cultures from labial tumors, it was not possible to detect HSV-specific antigens. Of the 25 cultures derived from cervical dysplasias, HSV antigens were found in only 3 cultures.

scholarly journals Comparison of a Multiplexed Herpes Simplex Virus Type-Specific Immunoglobulin G Serology Assay to Immunoblot, Western Blot, and Enzyme-Linked Immunosorbent Assays

2008 ◽  
Vol 16 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Thomas B. Martins ◽  
Ryan J. Welch ◽  
Harry R. Hill ◽  
Christine M. Litwin
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Western Blot ◽  
Immunoglobulin G ◽  
Multiplex Assay ◽  
Specific Alternative ◽  
Specific Antigens ◽  
Immunosorbent Assays ◽  
Simplex Virus ◽  
Hsv 1

ABSTRACT The human herpes simplex virus (HSV) is highly pathogenic, with infections caused by two distinct antigenic types, HSV-1 and HSV-2. Differentiation of antibodies to these specific antigens can provide useful information for the diagnosis of subclinical or undiagnosed HSV-2 infections, as well as for reducing the risk of maternal transfer of HSV to the neonate. In this study, a multiplex assay capable of concurrent detection of HSV-1 and -2 immunoglobulin G (IgG) antibodies was compared to immunoblot, Western blot, and enzyme-linked immunosorbent assays. Agreement of the multiplex assay was 95% or greater (n = 332) for both HSV-1 and -2 compared to the three assays. Sensitivities for HSV-1 ranged from 94.9 to 97.9%, with specificities of 93 to 97%. For HSV-2, the sensitivity and specificity ranges were 92.6 to 98.9% and 98.3 to 98.7%, respectively. Our studies show that the multiplexed microsphere-based assay offers a sensitive and specific alternative method for the detection HSV-1 and -2 type-specific antibodies. Advantages of the multiplex assay include multiple results per assay, the inclusion of internal controls for each specimen, and higher throughput of results.

scholarly journals Limits in Reliability of Glycoprotein G-Based Type-Specific Serologic Assays for Herpes Simplex Virus Types 1 and 2

1999 ◽  
Vol 37 (2) ◽  
pp. 376-379 ◽  
Author(s):  
D. Scott Schmid ◽  
Denise R. Brown ◽  
Rosane Nisenbaum ◽  
Rae Lyn Burke ◽  
D’Anna Alexander ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Western Blot ◽  
Validation Studies ◽  
Enzyme Linked Immunosorbent Assay ◽  
Western Blot Assay ◽  
Cross Sectional ◽  
Glycoprotein G ◽  
Simplex Virus ◽  
Hsv 1

Type-specific serologic assays for herpes simplex virus (HSV) types 1 and 2 based on glycoprotein G-1 (gG-1) (HSV-1) and gG-2 (HSV-2) discriminate between antibodies against HSV-1 and HSV-2. We previously developed a Western blot assay using gG-1 and gG-2 expressed in baculovirus, performed extensive validation studies, and determined that it was both sensitive and specific for type-specific detection of HSV antibody. Here we report that, among a cohort of Thai military recruits, the serostatus of some individuals changed from positive to negative over time (6.6% among those ever positive for HSV-1, and 14.9% among those ever positive for HSV-2). We tested a subset of these specimens in three other gG-based assays: an enzyme-linked immunosorbent assay, an immunoblot strip assay, and a Western blot assay. Positive-to-negative shifts occurred in every assay; the frequency of the shifts ranged from 6.1% to 21.2% of the specimen sets tested. There was only limited agreement among the assays concerning which individuals lost reactivity. This inaccuracy, exhibited by all of the assay protocols, was not predicted by validation studies employing specimens from cross-sectional studies and was most pronounced in HSV-2 testing. This argues for the inclusion of serial blood specimens in serologic assay validation procedures.

Development of immunofluorescent diagnostics for the determination of IgM and IgG antibodies to herpes simplex virus types 1 and 2

2021 ◽  
Vol 66 (5) ◽  
pp. 285-290
Author(s):  
N. N. Shershneva ◽  
S. S. Mardanly ◽  
I. N. Klenyaev ◽  
P. V. Samosadova
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Enzyme Linked Immunosorbent Assay ◽  
Central Nervous System Damage ◽  
Clinical Diagnoses ◽  
Simplex Virus ◽  
High Degree ◽  
Cytomegalovirus Infections ◽  
Hsv 1

The Russian kits «HSV-1-Fluorogen-screen» and «HSV-2-Fluorogen-screen» have been developed for the determination of antibodies M and G to herpes simplex virus types 1 and 2 by the immunofluorescence reaction. The kits were used to examine the positive and negative standard «EKOlab» panels sera and showed 100% sensitivity and specificity of the developed tests. 125 samples of blood serum from people with clinical diagnoses such as herpetic, cytomegalovirus infections, pyelonephritis, conjunctivitis and central nervous system damage were tested in parallel with using the enzyme-linked immunosorbent assay systems from different manufacturers and the developed tests «HSV-1-Fluorogen-screen» and «HSV-2-Fluorogen-screen». A high degree of matching of results with comparison sets was observed in examined samples. The developed diagnostics can be successfully used in clinical practice both for screening and for verification of results of the diagnosis of herpesvirus infections caused by herpes simplex virus types 1 and 2.

Seroprevalence of herpes simplex virus types 1 and 2 and correlates of infection in Jordan

2022 ◽  
pp. 095646242110601
Author(s):  
Samer F Swedan ◽  
Alia’ Darabseh
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Population Estimate ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Cross Sectional ◽  
Simplex Virus ◽  
Hsv 1

Background Herpes infections are common infections among populations. Herein, a cross-sectional study was used to determine the seroprevalence of herpes simplex virus type 1 (HSV-1) and herpes simplex virus type 2 (HSV-2) IgG antibodies and their association with potential infection risk factors among Jordanians. Methods A total of 759 serum samples were collected (January to February 2020) and analyzed for HSV-1 and HSV-2 IgG antibodies by enzyme-linked immunosorbent assay. Estimates for population seropositivity were determined by weighting the age-specific seroprevalence by the size of the population in each age stratum. Results The population estimate for HSV-1 seroprevalence was 75.3%. After adjustment for possible confounders, regression analysis revealed higher seroprevalence with increase in age ( p < 0.005) and low household income ( p = 0.002). The population estimate for HSV-2 seroprevalence was 2.9%. No significant differences in HSV-2 seroprevalence were observed in association with age, gender, family size, educational level, and socioeconomic status, likely due to low seropositivity. Conclusions Jordanians have high HSV-1 and low HSV-2 seroprevalence. Periodical studies might be needed to evaluate changes in HSV-1 and HSV-2 seroprevalence over time. This study provides essential epidemiological data for Jordan and the Middle East and North Africa region.

scholarly journals Performance of Two Commercial Glycoprotein G-Based Enzyme Immunoassays for Detecting Antibodies to Herpes Simplex Viruses 1 and 2 in Children and Young Adolescents

2002 ◽  
Vol 9 (5) ◽  
pp. 1124-1125 ◽  
Author(s):  
Charles T. Leach ◽  
Rhoda L. Ashley ◽  
Jacques Baillargeon ◽  
Hal B. Jenson
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Enzyme Linked Immunosorbent Assay ◽  
Aged Patients ◽  
Herpes Simplex Viruses ◽  
Glycoprotein G ◽  
Children And Young Adolescents ◽  
Simplex Virus ◽  
Hsv 1

ABSTRACT In 61 patients 1 to 14 years of age, the Gull/Meridian enzyme-linked immunosorbent assay (ELISA) had a sensitivity of 100% for herpes simplex virus type 1 (HSV-1) and specificities of 74% for HSV-1 and 48% for HSV-2. In 128 similarly aged patients, the HerpeSelect ELISA (Focus Technologies) showed sensitivities of 80% for HSV-1 and 88% for HSV-2, and specificities of 97% for HSV-1 and 100% for HSV-2.

scholarly journals Seroprevalence and characterisation of herpes simplex virus from human immunodeficiency virus in samples collected from two provinces in South Africa: a retrospective study

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 105
Author(s):  
Oluwafemi Samuel Obisesan ◽  
Nomathamsanqa Patricia Sithebe ◽  
Hazel Tumelo Mufhandu
Keyword(s):  
South Africa ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Goodness Of Fit ◽  
High Rate ◽  
Enzyme Linked Immunosorbent Assay ◽  
Goodness Of Fit Test ◽  
Simplex Virus ◽  
Hiv 1 ◽  
Hsv 1

Background: Herpes simplex virus (HSV) is a widely distributed human pathogen that is known for its ulcerative lesions at the infection site. HSV can cause persistent infection in the host that is often followed by a period of latency within the neurons. Considering the high rate of HIV infection in South Africa, it is important to assess the seroprevalence of HSV with a focus to determine the epidemiological association between HSV-DNA and HIV-1 in the population. Methods: A total of 44 sera samples were screened for HSV and HIV-1 using the highly sensitive enzyme-linked immunosorbent assay (ELISA). The ELISA positive samples were characterized using polymerase chain reaction (PCR) to confirm the positivity of both viruses and to further differentiate HSV into HSV-1 and -2. Thereafter, the samples were analysed for relatedness using phylogenetic analysis. Results: Of the 44 samples, 36 (81.8%) were positive for HIV-1, while 35 (79.5%) were positive for HSV when screened with ELISA kits. The PCR results, with the use of type specific primers, showed that 4/35 (11.4%) samples were specific for HSV-1 while 30/35 (85.7%) were specific for HSV-2. Statistical analysis performed using the chi-squared goodness-of-fit test showed that there is a significant relationship between HSV-2 and HIV-1 transmission. Conclusions: The prevalence of HSV in the population is high with an increased HSV-2 infection in women. Our study shows that some of the HSV-2 isolates are not related to the clinical isolate SD90e from South Africa, suggesting diversity in HSV-2 viral transmission.

scholarly journals Molecular Aspects of Herpes Simplex Virus I Latency, Reactivation, and Recurrence

1998 ◽  
Vol 9 (4) ◽  
pp. 541-562 ◽  
Author(s):  
C.S. Miller ◽  
R.J. Danaher ◽  
R.J. Jacob
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Recurrent Disease ◽  
Epithelial Tissue ◽  
Molecular Events ◽  
Current Thinking ◽  
Molecular Aspects ◽  
Simplex Virus ◽  
Relationship Of ◽  
Hsv 1

The application of molecular biology in the study of the pathogenesis of herpes simplex virus type 1 (HSV-1) has led to significant advances in our understanding of mechanisms that regulate virus behavior in sensory neurons and epithelial tissue. Such study has provided insight into the relationship of host and viral factors that regulate latency, reactivation, and recurrent disease. This review attempts to distill decades of information involving human, animal, and cell culture studies of HSV-1 with the goal of correlating molecular events with the clinical and laboratory behavior of the virus during latency, reactivation, and recurrent disease. The purpose of such an attempt is to acquaint the clinician/scientist with the current thinking in the field, and to provide key references upon which current opinions rest.

Clinical Manifestations of Primary Herpes Simplex Virus Type 1 Infection in a Closed Community

PEDIATRICS ◽  
1991 ◽  
Vol 87 (2) ◽  
pp. 152-158
Author(s):  
Kiyotaka Kuzushima ◽  
Hiroshi Kimura ◽  
Shinji Kido ◽  
Naoki Hanada ◽  
Motohiro Shibata ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Enzyme Linked Immunosorbent Assay ◽  
Herpetic Gingivostomatitis ◽  
Simplex Virus ◽  
Observation Period ◽  
Hsv 1

The clinical features and the molecular epidemiology of primary herpes simplex virus type 1 (HSV-1) infection among children younger than 3 years of age were investigated in day-care nursery. Serial sera were assayed for anti-HSV-1 glycoprotein B antibody by enzyme-linked immunosorbent assay. Serologic examinations revealed 55 cases of primary HSV infection during the observation period. Fifty-one of them (93%) had typical herpetic gingivostomatitis, showing a high rate of clinically overt infection. Four outbreaks of herpetic gingivostomatitis were observed during the observation period. Forty-one children were infected with HSV-1 in the outbreaks. The rates of infection in the susceptible children were 81%, 73%, 78%, and 100%, respectively, in the four outbreaks. Restriction endonuclease analysis of DNA of isolated HSV revealed that only one strain of HSV-1 had been transmitted among children for a long period.

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