An Electron Microscopic Study of Normal Blood and Bone Marrow in Pigs

1968 ◽  
Vol 5 (5) ◽  
pp. 451-470 ◽  
Author(s):  
Per H. J. Nafstad ◽  
Inger Nafstad
Keyword(s):  
Bone Marrow ◽  
Internal Structure ◽  
Animal Species ◽  
Bone Marrow Cells ◽  
Cell Types ◽  
Immature Stages ◽  
Electron Microscopic ◽  
Irregular Shapes ◽  
Microscopic Picture ◽  
Different Cell Types

The ultrastructure of blood and bone marrow cells in the normal pig was studied; the process of maturation of the different cell types was found to be essentially in accordance with that in other animal species. The eosinophilic granules had a pattern which differed from that in other mammals, being characterized by specific internal structure in the immature stages. During maturation, however, a homogenous appearance supervened. Moreover, the lymphocytic nuclei were found to have irregular shapes as compared with the light microscopic picture. The results are compared to some reported studies of other animal species.

scholarly journals The cultivation of bone marrow cells and cell lines on polymeric films

2011 ◽  
Vol 57 (5) ◽  
pp. 535-543
Author(s):  
M.S. Dolgikh ◽  
D.N. Livak ◽  
M.E. Krasheninnikov ◽  
N.A. Onishchenko
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Covalent Binding ◽  
Cell Types ◽  
Polymeric Films ◽  
Artificial Organ ◽  
Hep G2 ◽  
Different Cell Types ◽  
Cultural Media ◽  
Marrow Cells

The cultivation of multipotent mesenchymal stromal bone marrow cells and cells of A-431, MDCK, Vero, 3T3 and Hep-G2 was performed on polymeric films (PVA) with different hydrophobic fatty acid residues. The cells of different types grew on these films with different intensity, but in the most cases comparable with the cultivation control on usual plastic. The examined films were nontoxic to cells and sufficiently adhesive. They did not changed pH of cultural media, were optically transparent under microscope and comfortable in the experimental work. These films can be used as a model for the artificial organ construction. The covalent binding of different fatty acids to PVA shows possibility of the adaptable changes of films properties (hydrophobity and adhesiveness), and therefore possibility of the creation of optimal conditions for different cell types attachement and growth.

scholarly journals Dissecting the Role of Bone Marrow Stromal Cells on Bone Metastases

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Denise Buenrostro ◽  
Serk In Park ◽  
Julie A. Sterling
Keyword(s):  
Bone Marrow ◽  
Bone Metastases ◽  
Tumor Cells ◽  
Bone Marrow Cells ◽  
Current Knowledge ◽  
Metastatic Cancer ◽  
Cell Types ◽  
Bone Microenvironment ◽  
Or Gene ◽  
Different Cell Types

Tumor-induced bone disease is a dynamic process that involves interactions with many cell types. Once metastatic cancer cells reach the bone, they are in contact with many different cell types that are present in the cell-rich bone marrow. These cells include the immune cells, myeloid cells, fibroblasts, osteoblasts, osteoclasts, and mesenchymal stem cells. Each of these cell populations can influence the behavior or gene expression of both the tumor cells and the bone microenvironment. Additionally, the tumor itself can alter the behavior of these bone marrow cells which further alters both the microenvironment and the tumor cells. While many groups focus on studying these interactions, much remains unknown. A better understanding of the interactions between the tumor cells and the bone microenvironment will improve our knowledge on how tumors establish in bone and may lead to improvements in diagnosing and treating bone metastases. This review details our current knowledge on the interactions between tumor cells that reside in bone and their microenvironment.

scholarly journals Continuous Free-Flow Fractionation of Cellular Constituents in Rat Bone Marrow

Blood ◽  
1965 ◽  
Vol 25 (1) ◽  
pp. 63-72 ◽  
Author(s):  
HOWARD C. MEL ◽  
LINDA T. MITCHELL ◽  
BO THORELL
Keyword(s):  
Bone Marrow ◽  
Cell Types ◽  
Single Cell Suspension ◽  
Physical Classification ◽  
Normal Rat ◽  
Good Repeatability ◽  
Different Cell Types ◽  
Stable Flow ◽  
Rat Bone

Abstract A single-cell suspension of normal rat bone marrow is prepared mechanically. This suspension is continuously fractionated in free solution, under sedimentation rate conditions, using 1 g. only. With a sample flow of 2.2 x 106 cells/minute and a 32-minute steady-state residence time in the stable-flow free boundary (STAFLO) flow-cell, the cells exit almost entirely into 7 of the 12 collection bottles. Maximum numbers of different cell types are observed, with good repeatability, in approximately descending order from top to bottom as follows: erythrocytes, "erythroblasts," "immatures," "myelocytes," and mature granulocytes. Major changes are effected relative to the starting marrow composition, and large relative enrichments are achieved for certain cell types. In addition to the rapid, mild, preparative aspect of this study, nominal sedimentation rates can be assigned for the different collection fractions, in the range of 3 x 105 to 4 x 106 svedbergs, thus making a start on this kind of simple physical classification of the cellular elements in this complex tissue.

Electron Microscopic Study of the Graffi Virus in Bone Marrow of Leukemic Mice.

1966 ◽  
Vol 52 (1) ◽  
pp. 35-59 ◽  
Author(s):  
Natale Pennelli ◽  
Luciano Fiore-Donati ◽  
Luigi Chieco-Bianchi ◽  
Giuseppe Tridente
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Electron Microscopic ◽  
Virus Particles ◽  
Negative Stain ◽  
Cytoplasmic Vesicles ◽  
C57bl Mice ◽  
Ultrathin Sections ◽  
Number Of Particles ◽  
Marrow Cells

Bone marrow from C57BL mice with myeloid leukemia induced by Graffi virus has been studied with the electron microscope by ultrathin section and negative stain techniques. Virus particles were usually found in different types of bone marrow cells as well as in extracellular spaces. However, the highest number of particles in various stages of maturation was observed in the cytoplasm of megakaryocytes. Two main types of virus particle were found: the immature Al particle and the mature C particle. They were morphologically indistinguishable from other murine leukemogenic viruses. In partially purified preparations studied by negative staining, some of the particles which were not penetrated by PTA, frequently showed a tail-like structure of variable length. In ultrathin sections, particles were found to originate by budding from the cell membranes. Budding of particles was particularly evident in megakaryocytes and especially within the granules and cytoplasmic vesicles or in connection with the platelet demarcating membranes. The findings of a high number of virus particles in all stages of maturation in megakaryocytes together with a certain degree of megakaryocytosis observed in the bone marrow suggest that this type of cell is possibly one of the main source of production of the virus. A few particles resembling morphologically mycoplasma were detected within the cytoplasm of some immature bone marrow cells.

scholarly journals Augmented Production of Interleukin-6 by Normal Human Osteoblasts in Response to CD34+ Hematopoietic Bone Marrow Cells In Vitro

Blood ◽  
1997 ◽  
Vol 89 (4) ◽  
pp. 1165-1172 ◽  
Author(s):  
Russell S. Taichman ◽  
Marcelle J. Reilly ◽  
Rama S. Verma ◽  
Stephen G. Emerson
Keyword(s):  
Bone Marrow ◽  
Interleukin 6 ◽  
Transforming Growth Factor ◽  
Bone Marrow Cells ◽  
Hematopoietic Cells ◽  
Cell Types ◽  
Colony Stimulating Factor ◽  
Cd34 Cells ◽  
Stimulating Factor ◽  
Marrow Cells

Abstract Based on anatomic and developmental findings characterizing hematopoietic cells in close approximation with endosteal cells, we have begun an analysis of osteoblast/hematopoietic cell interactions. We explore here the functional interdependence between these two cell types from the standpoint of de novo cytokine secretion. We determined that, over a 96-hour period, CD34+ bone marrow cells had no significant effect on osteoblast secretion of granulocyte colony-stimulating factor, granulocyte-macrophage colony-stimulating factor, or transforming growth factor-β1 , but in some experiments minor increases in leukemia inhibitory factor levels were observed. However, when CD34+ bone marrow cells were cocultured in direct contact with osteoblasts, a 222% ± 55% (range, 153% to 288%) augmentation in interleukin-6 (IL-6) synthesis was observed. The accumulation of IL-6 protein was most rapid during the initial 24-hour period, accounting for nearly 55% of the total IL-6 produced by osteoblasts in the absence of blood cells and 77% of the total in the presence of the CD34+ cells. Cell-to-cell contact does not appear to be required for this activity, as determined by coculturing the two cell types separated by porous micromembranes. The identity of the soluble activity produced by the CD34+ cells remains unknown, but is not likely due to IL-1β or tumor necrosis factor-α, as determined with neutralizing antibodies. To our knowledge, these data represent the first demonstration that early hematopoietic cells induce the production of molecules required for the function of normal bone marrow microenvironments, in this case through the induction of hematopoietic cytokine (IL-6) secretion by osteoblasts.

Meis1-mediated apoptosis is caspase dependent and can be suppressed by coexpression of HoxA9 in murine and human cell lines

Blood ◽  
2005 ◽  
Vol 105 (3) ◽  
pp. 1222-1230 ◽  
Author(s):  
Peter J. Wermuth ◽  
Arthur M. Buchberg
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Cell Types ◽  
Selective Advantage ◽  
Myeloid Differentiation ◽  
Homeodomain Protein ◽  
Murine Bone Marrow ◽  
Differentiation Pathways

AbstractCoexpression of the homeodomain protein Meis1 and either HoxA7 or HoxA9 is characteristic of many acute myelogenous leukemias. Although Meis1 can be overexpressed in bone marrow long-term repopulating cells, it is incapable of mediating their transformation. Although overexpressing HoxA9 alone transforms murine bone marrow cells, concurrent Meis1 overexpression greatly accelerates oncogenesis. Meis1-HoxA9 cooperation suppresses several myeloid differentiation pathways. We now report that Meis1 overexpression strongly induces apoptosis in a variety of cell types in vitro through a caspase-dependent process. Meis1 requires a functional homeodomain and Pbx-interaction motif to induce apoptosis. Coexpressing HoxA9 with Meis1 suppresses this apoptosis and provides protection from several apoptosis inducers. Pbx1, another Meis1 cofactor, also induces apoptosis; however, coexpressing HoxA9 is incapable of rescuing Pbx-mediated apoptosis. This resistance to apoptotic stimuli, coupled with the previously reported ability to suppress multiple myeloid differentiation pathways, would provide a strong selective advantage to Meis1-HoxA9 coexpressing cells in vivo, leading to leukemogenesis.

scholarly journals Human eosinophil hematopoiesis studied in vitro by means of murine eosinophil differentiation factor (IL5): production of functionally active eosinophils from normal human bone marrow

Blood ◽  
1988 ◽  
Vol 71 (3) ◽  
pp. 646-651
Author(s):  
EJ Clutterbuck ◽  
CJ Sanderson
Keyword(s):  
Bone Marrow ◽  
Cell Types ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Electron Microscopic ◽  
Human Eosinophil ◽  
Interleukin 5 ◽  
Differentiation Factor ◽  
Microscopic Features

The production of human eosinophils in vitro from normal bone marrow by using murine eosinophil differentiation factor (mEDF/interleukin 5) is described. Eosinophil production was selective and first detectable after 14 days and reached a peak between 21 and 35 days when they were the predominant cell type (41% to 89%). Until day 14, all the eosinophils were typical myelocytes, developing thereafter into metamyelocytes and mature cells. All cell types had characteristic light- and electron-microscopic features, apart from the absence of granules with crystalline cores. The eosinophils produced were readily recovered, and both immature myelocytes and mature cells were functionally active in an antibody-dependent, cell-mediated cytotoxicity assay. mEDF added into the assay enhanced the cytotoxicity but to a lower degree than previously reported for peripheral blood eosinophils, which suggests that they may be partially activated. The possibility that eosinophils could be deactivated was tested by removing mEDF from the culture medium. The eosinophils retained viability and functional activity, however, and showed no increased ability to be activated by mEDF for up to six days after removing the mEDF. The liquid culture of human bone marrow was shown to be an alternative assay for eosinophil differentiation factors to colony formation.

scholarly journals CD34 expression by stromal precursors in normal human adult bone marrow

Blood ◽  
1991 ◽  
Vol 78 (11) ◽  
pp. 2848-2853 ◽  
Author(s):  
PJ Simmons ◽  
B Torok-Storb
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Complex Mixture ◽  
Antigen Expression ◽  
Cell Types ◽  
Stromal Precursors ◽  
Cd34 Antigen ◽  
Distinct Cell ◽  
Marrow Cells

Normal bone marrow cells were isolated by fluorescence-activated cell sorting (FACS) on the basis of CD34 antigen expression and then assayed in vitro for colonies of fibroblastic cells (fibroblast colony-forming units [CFU-F]). Greater than 95% of detectable CFU-F were recovered in the CD34+ population, while their numbers were markedly depleted in the CD34- population. Additional experiments showed that the majority of CFU-F exhibited high forward and perpendicular light scatter and low- density CD34 antigen. Growth of sorted cells in medium optimized for long-term marrow culture (LTMC) produced a complex mixture of adherent stromal elements including fibroblasts, adipocytes, smooth muscle cells, and macrophages. Monoclonal antibody STRO-1, which identifies bone marrow stromal cells, reacted with approximately 5% of CD34+ cells, which included all CFU-F and stromal precursors in LTMC. Experiments using soybean agglutinin (SBA) further showed that these stromal elements were restricted to a population of bone marrow cells with the phenotype CD34+/SBA+. These properties of stromal precursors are quite distinct from those of primitive hematopoietic progenitors, showing that although the precursors of the hematopoietic and stromal systems share expression of CD34, they are otherwise phenotypically distinct cell types.

scholarly journals Induction of unscheduled DNA synthesis in human myeloma cells

Blood ◽  
1980 ◽  
Vol 55 (2) ◽  
pp. 311-316
Author(s):  
R Lewensohn ◽  
U Ringborg
Keyword(s):  
Bone Marrow ◽  
Dna Synthesis ◽  
Bone Marrow Cells ◽  
Ultra Violet ◽  
Cell Types ◽  
Unscheduled Dna Synthesis ◽  
Myeloma Cells ◽  
Poorly Differentiated ◽  
The Relationship ◽  
Marrow Cells

Unscheduled DNA synthesis (UDS) induced by melphalan, nitrogen mustard and ultra-violet irradiation was studied in bone marrow cells from myeloma patients. In a previous study, normal bone marrow cells in various stages of maturation were found to display a gradual decrease in UDS parallel with the process of maturation. Myeloma cells showed a similar pattern. Poorly differentiated myeloma cells exhibited a similar level of UDS to myeloblasts and erythroblasts. Irrespective of which repair-inducing agent was used, the relationship between the levels of UDS in the various cell types was constant. This indicates that the differences in the level of UDS in the various cell types was not due to differences in the uptake of the repair-inducing agent.

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