Genotype Effects on β-Carotene Conversion to Vitamin A: Implications on Reducing Vitamin A Deficiency in the Philippines

Background: The study aimed to identify two beta-carotene 15,15′-monooxygenase (BCMO1) mutations, namely R267S and A379V, and determine their association with vitamin A status among Filipinos 6 to 19 years old respondents of the 2013 Philippine National Nutrition Survey living in the National Capital Region. Materials and Methods: This study followed cross-sectional design. Whole blood specimen was collected in the morning and was used as source of genomic DNA and serum for retinol concentration determination. Fisher exact test was performed to determine whether genotype frequencies were associated to retinol concentrations/vitamin A deficiency status. A level of P < .05 was identified as significant. Results: A total of 693 Filipino children and adolescents were included. Of the 693, there were at least 7.6% who bears the combined mutations for R267S + A379V. Association analysis showed that an inverse relationship exists between the A379V TT variant and vitamin A status. Although the exact role of these identified polymorphisms on retinol/carotenoid metabolism need to be confirmed in dedicated functional studies. Conclusion: This study has identified for the first time the presence of 2 nonsynonymous genetic variants/mutations in the coding region of BCMO1 gene. Interestingly, one of these two variants, the A379V T, was found to be associated with vitamin A status. It is, therefore, warranted to investigate the role of BCMO1 variants for the success of supplementation programs and fortification efforts among vulnerable populations in this region. Genetic variability should be considered for future provitamin A supplementation recommendations among children and adolescents in the Philippines.

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Prevalence and Determinants of the Co-Occurrence of Overweight or Obesity and Micronutrient Deficiencies among Adults in the Philippines: Results from a National Representative Survey

Nutrients ◽

10.3390/nu13072339 ◽

2021 ◽

Vol 13 (7) ◽

pp. 2339

Author(s):

Aileen R. de Juras ◽

Wan-Chen Hsu ◽

Susan C. Hu

Keyword(s):

Vitamin A ◽

Smoking Status ◽

Vitamin A Deficiency ◽

Wealth Quintile ◽

The Philippines ◽

Micronutrient Deficiencies ◽

Nutrition Survey ◽

Chi Square ◽

Double Burden ◽

Middle Income

The double burden of malnutrition (DBM) affects many low- and middle-income countries. However, few studies have examined DBM at the individual level, or undernutrition and overnutrition co-occurring within the same person. This study aims to investigate the prevalence and determinants of DBM among adults in the Philippines. Data from the 2013 National Nutrition Survey were used. The sample size in the analysis was 17,010 adults aged ≥20 years old, after excluding pregnant and lactating women. DBM was defined as the co-occurrence of overweight/obesity and anemia (definition #1), overweight/obesity and anemia or vitamin A deficiency (definition #2), and overweight/obesity and anemia or vitamin A deficiency or iodine insufficiency (definition #3). Anthropometric measurements and biochemical markers were used for DBM assessment. Data were analyzed using descriptive statistics, the chi-square test, and logistic regression by R software. Results showed that definition #3 is the predominant type of DBM (7.0%) in the general population, whereas the prevalence of DBM has increased to 23.7% in overweight/obese persons. Sex, age, educational attainment, marital status, household size, wealth quintile, and smoking status were the determinants of DBM. This study revealed that Filipino adults experience malnutrition critically and must be addressed through food and nutrition interventions.

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Research Recommendations for Applying Vitamin A-Labelled Isotope Dilution Techniques to Improve Human Vitamin A Nutrition

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000185 ◽

2014 ◽

Vol 84 (Supplement 1) ◽

pp. 52-59 ◽

Cited By ~ 5

Author(s):

Sherry A. Tanumihardjo ◽

Anura V. Kurpad ◽

Janet R. Hunt

Keyword(s):

Public Health ◽

Vitamin A ◽

Vitamin A Deficiency ◽

Human Subjects ◽

The Body ◽

Pool Size ◽

Serum Retinol ◽

Vitamin A Status ◽

Status Assessment ◽

Total Body

The current use of serum retinol concentrations as a measurement of subclinical vitamin A deficiency is unsatisfactory for many reasons. The best technique available for vitamin A status assessment in humans is the measurement of total body pool size. Pool size is measured by the administration of retinol labelled with stable isotopes of carbon or hydrogen that are safe for human subjects, with subsequent measurement of the dilution of the labelled retinol within the body pool. However, the isotope techniques are time-consuming, technically challenging, and relatively expensive. There is also a need to assess different types of tracers and doses, and to establish clear guidelines for the use and interpretation of this method in different populations. Field-friendly improvements are desirable to encourage the application of this technique in developing countries where the need is greatest for monitoring the risk of vitamin A deficiency, the effectiveness of public health interventions, and the potential of hypervitaminosis due to combined supplement and fortification programs. These techniques should be applied to validate other less technical methods of assessing vitamin A deficiency. Another area of public health relevance for this technique is to understand the bioconversion of β-carotene to vitamin A, and its relation to existing vitamin A status, for future dietary diversification programs.

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Prevalence of vitamin A deficiency and dietary inadequacy in Indian school-age children and adolescents

European Journal of Nutrition ◽

10.1007/s00394-021-02636-7 ◽

2021 ◽

Author(s):

Geereddy Bhanuprakash Reddy ◽

Tattari Shalini ◽

Santu Ghosh ◽

Raghu Pullakhandam ◽

Boiroju Naveen Kumar ◽

...

Keyword(s):

Vitamin A ◽

Children And Adolescents ◽

Vitamin A Deficiency ◽

School Age Children ◽

School Age ◽

Indian School

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Role of vitamin A deficiency in the pathogenesis of myeloproliferative disorders

Blood ◽

10.1182/blood.v98.5.1636 ◽

2001 ◽

Vol 98 (5) ◽

pp. 1636-1637

Author(s):

Muttuswamy Sivakumaran

Keyword(s):

Vitamin A ◽

Vitamin A Deficiency ◽

Myeloproliferative Disorders

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Vitamin A Supplementation and Child Morbidity and Mortality in Developing Countries

Food and Nutrition Bulletin ◽

10.1177/156482659401500414 ◽

1994 ◽

Vol 15 (4) ◽

pp. 1-9 ◽

Cited By ~ 27

Author(s):

George H. Beaton ◽

Reynaldo Martorell ◽

Kristan A. Aronson ◽

Barry Edmonston ◽

George McCabe. A. Catharine Ross ◽

...

Keyword(s):

Vitamin A ◽

Respiratory Infections ◽

Vitamin A Deficiency ◽

Meta Analysis ◽

Clinical Signs ◽

Vitamin A Status ◽

Child Morbidity ◽

Age Profile ◽

Mortality Trials ◽

Low Prevalence

A meta-analysis of eight mortality trials indicates that improving the vitamin A status of children aged six months to five years reduced mortality rates by about 23% in populations with at least low prevalence of clinical signs of vitamin A deficiency. The observed effect of supplementation, described in terms of relative risk (RR), was RR =0.77 (95% confidence interval 0.68–0.88; p < .001) and did not differ by sex or age. However, the number of lives saved was greater at younger ages because of higher mortality. A significant RR was shown for deaths attributed to diarrhoea and measles, but not for respiratory infection. Variability among the trials in effects was apparent, but attempts to explain it by descriptors of the population (baseline anthropometric status, prevalence of xerophthalmia, age profile, baseline mortality) were unsuccessful. Owing to the lack of data, firm conclusions could not be reached about effectiveness in children of less than six months and in settings where biochemical but not clinical evidence of vitamin A deficiency exists. Information about morbidity outcomes from about two dozen studies was reviewed. No consistent effects on frequency or prevalence of diarrhoeal and respiratory infections were found. Improvement in vitamin A status did appear to reduce severe morbidity, particularly in children with measles.

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Hubungan Penerapan Perilaku Kadarzi (Keluarga Sadar Gizi) dengan Status Gizi Balita di Kabupaten Tulungagung

Amerta Nutrition ◽

10.20473/amnt.v1i4.2017.379-388 ◽

2017 ◽

Vol 1 (4) ◽

pp. 379

Author(s):

Santik Wijayanti ◽

Triska Susila Nindya

Keyword(s):

Vitamin A ◽

Nutritional Status ◽

Vitamin A Deficiency ◽

Fisher Exact Test ◽

Height Measurement ◽

Cross Sectional ◽

Exact Test ◽

Children Under Five ◽

Under Five ◽

Nutritional Status Of Children

Background: Family conscious nutrition (Kadarzi) is an Indonesia government program that aims to address the nutrition problem. The family expected to be able to independently giving the best nutrition to improve health. Each Kadarzi indicator has an important role to resolve and prevent the occurrence of utrition problem as indicator of vitamin A supplementation is used to resolve vitamin A deficiency. Nutrition problems can be seen by using nutritional status. Children under five years are age-prone to experiencenutritional problem such as underweight, stunting, and wasting. Objective: The purpose of this study was to analyze the relationship between the application of Kadarzi behavior with the nutritional status of children under five (WAZ, HAZ, and WHZ). Method: The research was an analytic obsevation research, using cross sectional design. The sample consist of 72 toddlers in Salakkembang Village, Kalidawir Subdistrict, Tulungagung Regency. The data were collected by using weight and height measurement, also Kadarzi bahavior questionnaire. The data were analyzed using Fisher Exact test. Result: The results of the study showed that there was a correlation between Kadarzi behavior with nutitional status of toddlers based on WAZ (p=0.010), and HAZ (p=0.000) but not with WHZ (p=0.368). Conclusion: The better apllication of Kadarzi behavior, the better the nutritional status of toddlers WAZ and HAZ. Mothers should apply Kadarzi behavior to prevent toddlers from nutritional problems. ABSTRAK Latar belakang: Keluarga Sadar Gizi (Kadarzi) merupakan program pemerintah Indonesia yang bertujuan mengatasi masalah gizi. Keluarga diharapkan dapat secara mandiri mewujudkan keadaan gizi yang sebaik-baiknya untuk meningkatkan kesehatan. Setiap indikator Kadarzi memiliki peran penting untuk mengatasi dan mencegah terjadinya masalah gizi seperti indikator pemberian suplemen vitamin A digunakan untuk mengatasi kekurangan vitamin A. Masalah gizi dapat dilihat salah satunya dengan menggunakan status gizi. Balita merupakan usia yang rawan mengalami masalah gizi seperti underweight, stunting, dan wasting.Tujuan: Tujuan dari penelitian ini adalah menganalisis hubungan antara penerapan perilaku Kadarzi dengan status gizi balita (BB/U, TB/U, dan BB/TB).Metode: Penelitian ini merupakan penelitian observasinol analitik, dengan desain penelitian cross sectional. Sampel terdiri dari 72 balita di Desa Salakkembang, Kecamatan Kalidawir, Kabupaten Tulungagung. Pengumpulan data menggunakan penimbangan berat badan, tinggi badan, dan kuesioner perilaku Kadarzi. Data dianalisis menggunakan Fisher Exact test. Hasil: Hasil dari penelitian menunjukkan terhadap hubungan antara perilaku Kadarzi dengan status gizi balita BB/U (p=0,010) dan TB/U (p=0,000) namun tidak dengan BB/TB (p=0,368).Kesimpulan: Semakin baik penerapan perilaku Kadarzi maka semakin baik status gizi balita BB/U dan TB/U. Ibu dan seluruh anggota keluarga seharusnya menerapkan perilaku Kadarzi agar balita terhindar dari masalah gizi.

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Cassava with enhanced β-carotene maintains adequate vitamin A status in Mongolian gerbils (Meriones unguiculatus) despite substantial cis-isomer content

British Journal Of Nutrition ◽

10.1017/s0007114508184720 ◽

2009 ◽

Vol 102 (3) ◽

pp. 342-349 ◽

Cited By ~ 25

Author(s):

Julie A. Howe ◽

Bussie Maziya-Dixon ◽

Sherry A. Tanumihardjo

Keyword(s):

Vitamin A ◽

Vitamin A Deficiency ◽

Meriones Unguiculatus ◽

Provitamin A ◽

Carotenoid Content ◽

Mongolian Gerbils ◽

Serum Retinol ◽

Vitamin A Status ◽

Isomer Content ◽

Β Carotene

Efforts to increase β-carotene in cassava have been successful, but the ability of high-β-carotene cassava to prevent vitamin A deficiency has not been determined. Two studies investigated the bioefficacy of provitamin A in cassava and compared the effects of carotenoid content and variety on vitamin A status in vitamin A-depleted Mongolian gerbils (Meriones unguiculatus). Gerbils were fed a vitamin A-free diet 4 weeks prior to treatment. In Expt 1, treatments (ten gerbils per group) included 45 % high-β-carotene cassava, β-carotene and vitamin A supplements (intake matched to high-β-carotene cassava group), and oil control. In Expt 2, gerbils were fed cassava feeds with 1·8 or 4·3 nmol provitamin A/g prepared with two varieties. Gerbils were killed after 4 weeks. For Expt 1, liver vitamin A was higher (P < 0·05) in the vitamin A (1·45 (sd 0·23) μmol/liver), lower in the control (0·43 (sd 0·10) μmol/liver), but did not differ from the β-carotene group (0·77 (sd 0·12) μmol/liver) when compared with the high-β-carotene cassava group (0·69 (sd 0·20) μmol/liver). The bioconversion factor was 3·7 μg β-carotene to 1 μg retinol (2 mol:1 mol), despite 48 % cis-β-carotene [(Z)-β-carotene] composition in cassava. In Expt 2, cassava feed with 4·3 nmol provitamin A/g maintained vitamin A status. No effect of cassava variety was observed. Serum retinol concentrations did not differ. β-Carotene was detected in livers of gerbils receiving cassava and supplements, but the cis-to-trans ratio in liver differed from intake. Biofortified cassava adequately maintained vitamin A status and was as efficacious as β-carotene supplementation in the gerbil model.

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Vitamin A Status and Hepatic Drug Metabolism in the Rat

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y73-002 ◽

1973 ◽

Vol 51 (1) ◽

pp. 6-11 ◽

Cited By ~ 51

Author(s):

G. C. Becking

Keyword(s):

Vitamin A ◽

Drug Metabolism ◽

Vitamin A Deficiency ◽

Hepatic Drug Metabolism ◽

Deficient Diet ◽

Hepatic Drug ◽

Vitamin A Status ◽

Cytochrome P 450 ◽

Liver Vitamin

The effect of vitamin A status on hepatic drug metabolism was studied in rats. Animals were fed diets with and without vitamin A for 20 and 25 days. Weight gains of control and deficient animals were not significantly different, whereas liver vitamin A levels had decreased to less than 10% of control animals after 20 days and were essentially zero after eating the deficient diet for 25 days. Aniline metabolism in vitro and aminopyrine metabolism in vitro and in vivo were significantly lower in male weanling rats fed a vitamin A deficient diet for 20 days. No alteration in in vitro p-nitrobenzoic acid metabolism was noted after 25 days on the test. Vitamin A deficiency did not alter microsomal protein levels or cytochrome c reductase activity but deficient animals did have a lower microsomal cytochrome P-450 content. Hepatic enzyme activities and cytochrome P-450 levels were restored to values approaching those found in control animals by feeding vitamin A deficient rats the vitamin A containing diet for 21 days. Liver vitamin A levels were markedly increased after re-feeding studies but were still significantly lower than control animals.

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