Protective role of caffeic acid phenethyl ester and erdosteine on activities of purine-catabolizing enzymes and level of nitric oxide in red blood cells of isoniazid-administered rats*

2008 ◽  
Vol 24 (8) ◽  
pp. 519-524 ◽  
Author(s):  
HR Yilmaz ◽  
E Uz ◽  
O Gökalp ◽  
N Özçelik ◽  
E Çiçek ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Red Blood Cells ◽  
Caffeic Acid ◽  
Blood Cells ◽  
Tap Water ◽  
Treated Group ◽  
Caffeic Acid Phenethyl Ester ◽  
Male Rats ◽  
Control Group

The aim of this experimental study was to investigate the possible role of nitric oxide (NO) and the activities of adenosine deaminase (ADA) and xanthine oxidase (XO) in the pathogenesis of isoniazid (INH)-induced oxidative damage in red blood cells (RBCs), and also to show the effect of caffeic acid phenethyl ester (CAPE) and erdosteine, antioxidants, in decreasing this toxicity. A total of 25 adult male rats were divided into four experimental groups as follows: control group ( n = 7), INH-treated group ( n = 6), INH + CAPE–treated group ( n = 6), and INH + erdosteine–treated group ( n = 6). INH, INH-CAPE, and INH-erdosteine–treated groups were treated orally with INH 50 mg/kg daily and with the tap water for 15 days. Control group was given only tap water. CAPE was intraperitoneally injected for 15 days at a dose of 10 μmol/kg. Erdosteine was treated orally for 15 days at a dose of 10 mg/kg/day. The injection of INH led to a significant increase in the activities of ADA, XO, and NO levels in RBCs of rats. Co-treatment with CAPE caused a significant decrease in the activities of ADA and XO and the levels of NO in RBCs. In addition, co-treatment with erdosteine caused a significant decrease in the activities of ADA and XO and the levels of NO in RBCs. The results of this study showed that ADA, XO, and NO may play an important role in the pathogenesis of INH-induced oxidative stress in RBCs. CAPE and erdosteine may have protective potential in this process and they may become a promising drug in the prevention of this undesired side effect of INH.

scholarly journals Biochemical and Haematological Study in Rats Exposed to Cadmium Chloride in Drinking Water

2007 ◽  
Vol 31 (2) ◽  
pp. 15-29
Author(s):  
Hanaa A. A.
Keyword(s):  
Drinking Water ◽  
Blood Cells ◽  
Cadmium Chloride ◽  
Tap Water ◽  
White Blood Cells ◽  
Haemoglobin Concentration ◽  
Treated Group ◽  
Physiological Effect ◽  
Male Rats ◽  
Control Group

The objective of this study was investigate the Physiological effect ofdifferent concentrations of cadmium chloride in drinking water on somebiochemical and haematological parameters of male rats. Animals in thisexperiment were randomly divided into four equal groups and treated for 15weeks as follows : Rats in control group were offered ordinary tap water ,while animal in M1 , M2 , and M3 were received 10 , 20 , 30 ppm Cdcl2 indrinking water, respectively. The activity of Alanine aminotransferase(ALP), Aspartate aminotrasferase (AST) and alkaline phosphatase (ALP) inserum were measured. Further mor, hemoglobin concentration (Hb) andwhite blood cells count (WBCs) were detected. The result revealed thataddition of cadmium chloride in different concentrations in drinking watercaused a significant increase in activity of serum of ALT in treated group(M1 , M2 , M3 ) as compound with control. Within the time the activity ofserum AST was significantly increased in three treated group as comparedwith pretreatment period. On the other hand , significant increase in serumALP concentration were show in both M1 and M3 treated groups at 12th and15th week of the treatment as compared with control group. Significantdecrease in haemoglobin concentration were observed in M1 , M2 , M3 from9th week to the end of experiment comparing to control. While cadmiumchloride treatment caused significant increase in total white blood cells countin treated groups as compared with control group. On Conclusion, it seemslikely that exposure of male rats to cadmium chloride at level above thepermissive one had induced clear biochemical and haematological changes.

Ameliorating role of caffeic acid phenethyl ester (CAPE) against isoniazid-induced oxidative damage in red blood cells

2006 ◽  
Vol 290 (1-2) ◽  
pp. 55-59 ◽  
Author(s):  
Osman Gokalp ◽  
Efkan Uz ◽  
Ekrem Cicek ◽  
H. Ramazan Yilmaz ◽  
Mehmet Kaya Ozer ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Red Blood Cells ◽  
Caffeic Acid ◽  
Blood Cells ◽  
Caffeic Acid Phenethyl Ester

scholarly journals The Effect of Experimental Escherichia coli Infection on Some Blood Parameters and Histological Changes in Male Rats

2011 ◽  
Vol 35 (2) ◽  
pp. 22-27
Author(s):  
Hayder AL – Zamely
Keyword(s):  
Escherichia Coli ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Histopathological Examination ◽  
Hemoglobin Concentration ◽  
Blood Parameters ◽  
Infected Group ◽  
Male Rats ◽  
Control Group ◽  
Renal Tubules

This study was carried out to investigate the effect of experimental infection by Escherichia coli on some blood parameters and histopathological changes in male rats to reach this objective twenty male rats (Rattus norvigicus) were divided into two groups, housed under same conditions of water, feed, temperature, light and humidity. The first one served as control group, while the second was experimentally infected with Ecoli by administration of 2ml / rat of (1×109) colony forming unit/ml of E-coli. Five days later blood were obtained from animals by cardiac puncture to measure the total and differential white blood cells count hemoglobin concentration packed cells volume red blood cells count, and thrombocytes count. One animal from each group was killed and histopathological examination was done for intestine kidney liver and spleen to detect the effect of infection on these organs. The results revealed a significant increase in total leukocytes count (P<005) in infected group while the differential count showed a significant increase in neutrophils and significant decrease in lymphocytes (P< 0.05) in same group. There were a significant decrease in hemoglobin concentration packed cell volume and non significant decrease in red blood cells count. While thrombocytes count showed a significant decrease in infected group compared with control group (P<0.05). The histopathological examination of infected male rats intestine was demonstrated mild diffuse inflammation which include increased numbers of neutrophile in tissue intraepithelial lymphocytes and goblet cells In kidney renal tubules showed extensive epithelial swelling with decreased lumen space and generalized necrotic changes with interstitial hemorrhage in renal cortex. In liver hepatocytes were swollen with decreased sinusoidal spaces and widely distributed necrotic foci were seen. There is extensive splenic necrosis with infiltration of leukocytes from white pulp to red pulp of spleen.

Effect Of Citrullus Lanatus Juice On Hemoglobin, Red Blood Cells And Liver Enzyme; An Experimental Study

2019 ◽  
Vol 09 (04) ◽  
pp. 257-260
Author(s):  
Shahid Ali ◽  
Nuzhat Sultana ◽  
Muslim Abbas ◽  
Zareen Naz ◽  
Muhammad Akbar Hassan ◽  
...  
Keyword(s):  
Experimental Study ◽  
Red Blood Cells ◽  
Liver Enzyme ◽  
Blood Cells ◽  
Citrullus Lanatus ◽  
Treated Group ◽  
Control Group ◽  
Group A ◽  
Group B ◽  
Post Hoc

Objective: To determine effect of citrullus lanatus juice on hemoglobin and Serum Glutamic Pyruvic Transaminase (SGPT) level. Study design and setting: It was an experimental study conducted on healthy rabbits for sixty days in the research department of pharmacy University of Karachi. Methodology: Current study was planned to evaluate effect of Citrullus lanatus juice on red blood cells, hemoglobin and liver enzyme. 60 days study was performed at 2 different doses i.e 3and 6ml/kg on rabbits. These rabbits were from either gender and were divided into 3 groups their weight ranges from 1200 to 1800 grams. Group A is control group (Normal saline 6ml/kg), Group B is treated group (3 ml/kg), Group C is treated group (6ml/kg). After taking mean of all values they are compared with control group. Significance of mean can be estimated by Tukes Post Hoc Test. P<0.05 estimated as significant. Results: It was found that count of red blood cells rises significantly along with rise in hemoglobin level. As far as liver enzyme serum glutamate pyruvate transaminase (SGPT) was concerned its concentration decreases slightly. Conclusion: Citrullus lanatus juice contain ingredients which are important for RBC hemoglobin and synthesis .It also contains important antioxidants that have organoprotective role due to which SGPT level decreases even in healthy animals as compared to control groups

scholarly journals Protective role of ceftriaxone plus sulbactam with VRP1034 on oxidative stress, hematological and enzymatic parameters in cadmium toxicity induced rat model

2012 ◽  
Vol 5 (4) ◽  
pp. 192-200 ◽  
Author(s):  
Vivek Kumar Dwivedi ◽  
Anuj Bhatanagar ◽  
Manu Chaudhary
Keyword(s):  
Free Radical ◽  
Tissue Injury ◽  
Cadmium Toxicity ◽  
Treated Group ◽  
Protective Role ◽  
Enzymatic Activities ◽  
Exposed Group ◽  
Male Rats ◽  
Control Group

ABSTRACT We investigated the protective role of ceftriaxone plus sulbactam with VRP1034 (Elores) on hematological, lipid peroxidation, antioxidant enzymatic activities and Cd levels in the blood and tissues of cadmium exposed rats. Twenty-four male rats were divided into three groups of eight rats each. The control group received distilled water whereas group II received CdCl2 (1.5 mg/4 ml/body weight) through gastric gavage for 21 days. Group III received CdCl2 and was treated with ceftriaxone plus sulbactam with VRP1034 for 21 days. The hematological, biochemical, lipid per-oxidation levels and enzymatic parameters were measured in plasma and tissues (brain, liver and kidney) of all groups. The Cd, Zn and Fe levels were measured in blood and tissues of all groups. Our findings showed significantly decreased cadmium (p<0.001), malonaldialdehyde (p<0.001) and myloperoxidase (MPO) levels along with significantly increased hemoglobin (p<0.01), RBC (p<0.05), hematocrit (p<0.05) levels and all antioxidant enzymatic activities (SOD, CAT, GR, GPx) in plasma and tissues of ceftriaxone plus sulbactam with VRP1034 treated group as compared to cadmium exposed group. Delta aminolevulinate dehydratase (δ-ALAD) activity was significantly (p<0.001) increased in the blood of ceftriaxone plus sulbactam with VRP1034 treated group as compared with cadmium exposed group. The levels of hepatic and renal parameters were significantly (p<0.001) decreased in ceftriaxone plus sulbactam with VRP1034 treated group as compared to cadmium exposed group. These findings indicate that ceftriaxone plus sulbactam with VRP1034 acts as a potent free radical scavenger and exhibits metal chelating properties that reduce free radical mediated tissue injury and prevent dysfunction of hepatic and renal organs during metal intoxication.

scholarly journals Role of membrane permeability and extracellular diffusion in nitric oxide transport to the red blood cells

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
Prabhakar Deonikar ◽  
Mahendra Kavdia
Keyword(s):  
Nitric Oxide ◽  
Red Blood Cells ◽  
Membrane Permeability ◽  
Blood Cells

scholarly journals Caffeine intake decreases oxidative stress and inflammatory biomarkers in experimental liver diseases induced by thioacetamide: Biochemical and histological study

2017 ◽  
Vol 30 (1) ◽  
pp. 13-24 ◽  
Author(s):  
Mona G Amer ◽  
Nehad F Mazen ◽  
Ahmed M Mohamed
Keyword(s):  
Oxidative Stress ◽  
Liver Disease ◽  
Tap Water ◽  
Histological Study ◽  
Treated Group ◽  
Male Rats ◽  
Caffeine Intake ◽  
Control Group ◽  
Collagen Type Iv ◽  
Elevated Liver Enzymes

Liver disease remains a significant global health problem. Increased caffeine consumption has been associated with a lower prevalence of chronic liver disease. This study aimed to investigate the modifying effects of caffeine on liver injury induced by thioacetamide (TAA) administration in male rats and the possible underlying mechanisms. Forty adult male rats were equally classified into four groups: control group, received only tap water; caffeine-treated group, received caffeine (37.5 mg/kg per day); TAA-treated group, received intraperitoneal (i.p.) TAA (200 mg/kg b.w.) twice a week; and caffeine + TAA-treated group, received combined TAA and caffeine in the same previous doses. After eight weeks of treatment, blood samples were collected for biochemical analysis and liver specimens were prepared for histological and immunohistochemical studies and for assessment of oxidative stress. TAA induced liver toxicity with elevated liver enzymes and histological alterations, fatty changes, apoptosis, and fibrosis evidenced by increased immunohistochemical reaction to matrix metalloproteinase-9 (MMP-9) and collagen type IV in hepatocytes. Also, the levels of pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) in serum were significantly elevated. Co-treatment with caffeine and TAA restored normal liver structure and function. Caffeine provided an anti-fibrogenic, anti-inflammatory, and antioxidant effect that was associated with recovery of hepatic histological and functional alterations from TAA-induced hepatotoxicity.

Protective role of caffeic acid on lambda cyhalothrin-induced changes in sperm characteristics and testicular oxidative damage in rats

2011 ◽  
Vol 28 (7) ◽  
pp. 639-647 ◽  
Author(s):  
Fatma Ben Abdallah ◽  
Hamadi Fetoui ◽  
Nassira Zribi ◽  
Feiza Fakhfakh ◽  
Leila Keskes
Keyword(s):  
Oxidative Damage ◽  
Caffeic Acid ◽  
Reproductive System ◽  
Male Fertility ◽  
Treated Group ◽  
Protective Role ◽  
Male Reproductive System ◽  
Male Rats ◽  
Lambda Cyhalothrin

The synthetic pyrethroids are expected to cause deleterious effects on most of the organs and especially on the male reproductive system. The current study was performed to assess the adverse effect of lambda cyhalothrin (LC) on reproductive organs and fertility in male rats and to evaluate the protective role of caffeic acid phenethyl ester (CAPE) in alleviating the detrimental effect of LC on male fertility. A total of 48 male rats were divided into 4 groups (12 rats each): control group received distilled water ad libitum and 1 ml of vehicle solution given intraperitoneally (i.p.); CAPE-treated group received a single i.p. dose of CAPE (10 μmol kg−1 day−1); LC-treated group received 668 ppm of LC through drinking water; and CAPE + LC-treated group received an i.p. injection of CAPE (10 μmol kg−1 day−1) 12 h before the LC administration. The experiment was conducted for 10 consecutive weeks. LC caused a significant increase in testicular malondialdehyde, catalase, superoxide dismutase, glutathione- S-transferase activities, and sperm abnormalities and a significant reduction in testicular glutathione concentration, sperm count, sperm motility, and a live sperm percentage. Conversely, treatment with CAPE improved the reduction in the sperm characteristics, LC-induced oxidative damage of testes and the testicular histopathological alterations. Results indicate that LC exerts significant harmful effects on the male reproductive system and that CAPE reduced the deleterious effects of LC on male fertility.

The Role of Red Blood Cells and Hemoglobin–Nitric Oxide Interactions on Blood Flow

2008 ◽  
Vol 38 (2) ◽  
pp. 125-126 ◽  
Author(s):  
Mark T. Gladwin ◽  
Rakesh P. Patel
Keyword(s):  
Nitric Oxide ◽  
Blood Flow ◽  
Red Blood Cells ◽  
Blood Cells

The activities of liver adenosine deaminase, xanthine oxidase, catalase, superoxide dismutase enzymes and the levels of malondialdehyde and nitric oxide after cisplatin toxicity in rats: protective effect of caffeic acid phenethyl ester

2005 ◽  
Vol 21 (1-2) ◽  
pp. 67-73 ◽  
Author(s):  
H Ramazan Yilmaz ◽  
Sadik Sogut ◽  
Birsen Ozyurt ◽  
Fikret Ozugurlu ◽  
Semsettin Sahin ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Superoxide Dismutase ◽  
Xanthine Oxidase ◽  
Adenosine Deaminase ◽  
Caffeic Acid ◽  
Liver Tissue ◽  
Caffeic Acid Phenethyl Ester ◽  
Control Group ◽  
Albino Rats ◽  
Significant Change

The aim of this experimental study was to investigate the effects of caffeic acid phenethyl ester (CAPE), an antioxidant agent, on cisplatin-induced hepatotoxicity through adenosine deaminase (AD), xanthine oxidase (XO), catalase (CAT), superoxide dismutase (SOD) activities and malondialdehyde (MDA) and nitric oxide (NO) levels in liver tissue of rats. Wistar albino rats were divided into three groups: control group (n-6), cisplatin group (n-9) and CAPE+cisplatin group (n-8). All the chemicals used were applied intraperitoneally. Spectrophotometric methods were used to determine the activities of the above-mentioned enzymes in the liver tissue. NO level and XO activity were found to be increased in the cisplatin group compared to the control group. NO level was found to be decreased in the cisplatin+CAPE group in comparison with the cisplatin group. There was no significant change in the activity of XO between the cisplatin and cisplatin+CAPE groups. The activity of SOD was lower in the cisplatin group than both the control and cisplatin+CAPE groups. There was no significant change in the activity of CAT between the control and cisplatin groups. CAT activity was increased in the cisplatin+CAPE group compared to the cisplatin group. The AD activity and MDA level remained unchanged in all groups. The results obtained suggested that CAPE significantly attenuated the hepatotoxicity as an indirect target of cisplatin in an animal model of cisplatin-induced nephrotoxicity.

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