Effect Of Citrullus Lanatus Juice On Hemoglobin, Red Blood Cells And Liver Enzyme; An Experimental Study

2019 ◽  
Vol 09 (04) ◽  
pp. 257-260
Author(s):  
Shahid Ali ◽  
Nuzhat Sultana ◽  
Muslim Abbas ◽  
Zareen Naz ◽  
Muhammad Akbar Hassan ◽  
...  
Keyword(s):  
Experimental Study ◽  
Red Blood Cells ◽  
Liver Enzyme ◽  
Blood Cells ◽  
Citrullus Lanatus ◽  
Treated Group ◽  
Control Group ◽  
Group A ◽  
Group B ◽  
Post Hoc

Objective: To determine effect of citrullus lanatus juice on hemoglobin and Serum Glutamic Pyruvic Transaminase (SGPT) level. Study design and setting: It was an experimental study conducted on healthy rabbits for sixty days in the research department of pharmacy University of Karachi. Methodology: Current study was planned to evaluate effect of Citrullus lanatus juice on red blood cells, hemoglobin and liver enzyme. 60 days study was performed at 2 different doses i.e 3and 6ml/kg on rabbits. These rabbits were from either gender and were divided into 3 groups their weight ranges from 1200 to 1800 grams. Group A is control group (Normal saline 6ml/kg), Group B is treated group (3 ml/kg), Group C is treated group (6ml/kg). After taking mean of all values they are compared with control group. Significance of mean can be estimated by Tukes Post Hoc Test. P<0.05 estimated as significant. Results: It was found that count of red blood cells rises significantly along with rise in hemoglobin level. As far as liver enzyme serum glutamate pyruvate transaminase (SGPT) was concerned its concentration decreases slightly. Conclusion: Citrullus lanatus juice contain ingredients which are important for RBC hemoglobin and synthesis .It also contains important antioxidants that have organoprotective role due to which SGPT level decreases even in healthy animals as compared to control groups

scholarly journals AB0-incompatibility of mother and fetus: the role of anti-glycan alloantibodies in the hemolytic disease of newborns

2021 ◽  
Vol 23 (1) ◽  
pp. 17-34
Author(s):  
P. S. Obukhova ◽  
A. V. Kachanov ◽  
N. A. Pozdnyakova ◽  
M. M. Ziganshina
Keyword(s):  
Red Blood Cells ◽  
Blood Group ◽  
Blood Cells ◽  
Hemolytic Disease ◽  
Disease Condition ◽  
Mother And Child ◽  
Group A ◽  
Group B ◽  
Maternal Igg

The mother and fetus incompatibility due to Rh-factor, blood group or other blood factors can lead to hemolytic disease of the fetus and newborn (HDN). HDN is a clinical disease condition of the fetus and newborn as a result of hemolysis, when maternal IgG alloantibodies cross the placenta and destroy the red blood cells of the fetus and newborn. The child disease begins in utero and can dramatically increase immediately after birth. As a result, hyperbilirubinemia and anemia develop, that can lead to abortions, serious complications, or death of the neonates in the absence of proper therapy. The range of HDN has changed significantly now compared to previous decades. Half a century ago, HDN was considered an almost complete synonym of RhD-alloimmunization, and this was a frequent problem for newborns. By now due to the high effective of Rh-conflict prevention, immunological AB0-conflicts have become the most common cause of HDN. The review aimes to one of the main causes of jaundice and anemia in neonates at present, i.e. HDN due to immunological AB0-conflict of mother and newborn (AB0-HDN). The main participants of the AВ0- incompatibility mother and child are considered, namely A- and B-glycans, as well as the corresponding anti-glycan alloantibodies. Close attention is paid to the structure features of glycan alloantigens on the red blood cells of the fetus and adult. The possible correlation of the frequency and severity of HDN with the blood group of mother and child, as well as with the titer of maternal alloantibodies, has been considered. The influence of immunoglobulin G subclasses on the AB0-HDN development has been evaluated. In most cases, AB0-HDN appear when the mother has the blood group 0, and the fetus has the group A (subgroup A1) or the group B. Other rare incidences of AB0-incompatibility with severe course are occurred. As a whole the etiology of AB0-HDN is complex and the HDN severity is influenced by many factors. The authors have analyzed statistical data, as well as the prevalence of AB0-incompatibility and AB0-HDN in various regions of the world. Current approaches to the diagnosis of AB0-HDN are discussed in addition. By now the problems of AB0- HDN occurrence and developing of ways to overcome this disease remain relevant.

scholarly journals Ameliorative effects of Spinacia oleracea on sperm morphology, count, and motility by normalizing the obesity-induced oxidative stress in Sprague Dawley rats

2020 ◽  
Vol 14 (03) ◽  
pp. 124-127
Author(s):  
Somia Iqbal ◽  
Noman Sadiq ◽  
Saad Siddiqui ◽  
Hira Iqbal
Keyword(s):  
Sperm Concentration ◽  
Treated Group ◽  
Sperm Parameters ◽  
Control Group ◽  
Sprague Dawley ◽  
Normal Morphology ◽  
Sprague Dawley Rats ◽  
Group A ◽  
Group B ◽  
Experimental Group

Background: Obesity is a prevailing metabolic disorder that affects the functioning of the male reproductive system. Excessive adipose tissue enhances reactive oxygen species generation and is linked with male infertility. Spinach has demonstrated antioxidant effects. The present study was conducted to determine the antioxidant effects of spinach on sperm parameters in obese Sprague Dawley rats. Subjects and methods: This randomized control study was conducted at the animal house of the National Institute of Health Islamabad, Islamic International Medical College, Cosmesurge International Hospital, Rawalpindi, and Apollo lab, Islamabad, Pakistan from April 2016 to March 2017. Forty male Sprague Dawley rats having an age of 8 weeks and weight 160-200g were tagged from number 1 to 40. Every third rat was randomly allocated to control Group A (n=13) and remaining into the Experimental group (n=27). Rats of control Group A was given a standard diet while a high-fat diet was given to Experimental group rats to induce obesity for the duration of six weeks. Weight (g) was measured weekly and obesity was confirmed when rats attain more than 20% weight when compared with that of rats of control Group A. Then, after obesity induction, the experimental group was alienated into the obesity control group (Group B) and spinach treated group (Group C). For sample, rats of Group A and Group B were sacrificed, and the cauda epididymis of each rat was placed in a Petri dish containing normal saline and cut into pieces to allow the release of sperm and then sperm parameters (sperms concentration, motility, and morphology) were recorded under the microscope. Then, spinach (5% hot water extract) along with the persistence of fat diet was administered to Group C for 4 weeks and finally, sperm parameters were measured in this group. Results: Sperm concentration/ml, motility (%), and normal morphology (%) of Group B rats were significantly decreased as compared to Group A rats. However, sperm concentration/ml, motility (%), and normal morphology (%) of Group C (spinach treated group) rats was significantly increased (p<0.001) as compared to Group B (obesity control group) rats after administering spinach. Conclusion: The addition of Spinach in a normal diet regimen restores normal sperm morphology, improves sperm motility and concentration.

scholarly journals Cerebral Hemodynamics and Cognitive Function in Cirrhotic Patients with Hepatic Encephalopathy

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Yuqing Zhou ◽  
Qian Dong ◽  
Rong Zhang ◽  
Shunfeng Zhou ◽  
Linqiang Li ◽  
...  
Keyword(s):  
Cognitive Function ◽  
Treated Group ◽  
Cerebral Hemodynamics ◽  
Control Group ◽  
Group 4 ◽  
Group A ◽  
Cirrhotic Patients ◽  
Group 3 ◽  
Group 2 ◽  
Group B

Aims.To investigate cerebral hemodynamics in cirrhotic patients with HE and to observe effects of treatment in cerebral hemodynamics and correlations among ammonia, cerebral hemodynamics, and cognitive function.Methods. There were four groups: healthy controls (group 1), cirrhosis without HE (group 2), cirrhosis with MHE (group 3), and cirrhosis with OHE (group 4). Ammonia and cerebral hemodynamics (by TCD) were assessed. Patients in group 3 were subsequently randomized to two subgroups: the control (group A) and the treated (group B, treated with lactulose for two months), and they were retested for ammonia and TCD after treatment. Results. Ammonia,Vm,Vd, PI, and RI were statistically different before treatment, and ammonia, PI, and RI levels paralleled the severity of HE (P<0.05). In group B,Vdincreased and ammonia, PI, and RI declined following treatment (P<0.05), while there were no differences in group A (P>0.05). Correlations were found between ammonia andVd, PI, RI, NCT-A, and DST and also found betweenVd, PI, RI, and NCT-A and DST (P<0.05).Conclusions. This study revealed that cerebral hemodynamics were related to the severity of HE and cerebral autoregulation was impaired. There were tight correlations among ammonia, cerebral hemodynamics, and cognitive function, and, following treatment, cerebral hemodynamics improved.

scholarly journals Comparison of the effect of two anti-adhesion materials on the formation of postoperative adhesions after myomectomy – an experimental study on rabbit does

2021 ◽  
Vol 90 (4) ◽  
pp. 407-414
Author(s):  
Soňa Pánková ◽  
Andrea Nečasová ◽  
Jana Lorenzová ◽  
Vladimír Jekl ◽  
Alois Nečas ◽  
...  
Keyword(s):  
Experimental Study ◽  
Statistical Evaluation ◽  
Scoring Systems ◽  
Control Group ◽  
Routine Practice ◽  
Postoperative Adhesions ◽  
Group A ◽  
Level Of Significance ◽  
Group B ◽  
High Level

This study compares the effect of Hyalobarrier Gel and 4DryField® PH on the formation of postoperative adhesions after myomectomy in an experimental study on rabbit does. Thirty experimental animals were divided into three groups: group A – control, group B – use of 4DryField® PH, and group C – use of the Hyalobarrier Gel. A simulated myomectomy was performed in each group. The animals were euthanised 14 days after the procedure and the antiadhesive effect of the above-mentioned preparations was evaluated according to the criteria. Statistical evaluation clearly showed differences in the antiadhesive effect in the scoring systems for groups B and C compared to control group A at a high level of significance. We demonstrated an obvious, statistically confirmed antiadhesive effect of the Hyalobarrier Gel and 4DryField® PH in an experimental model of the rabbit uterus. Comparing both preparations, the Hyalobarrier Gel shows a higher level of effectiveness. Based on the results of the experiment, both preparations can be unambiguously recommended for routine practice.

scholarly journals Antidiabetogenic impact of bitter melon (Momordica charantia) and garlic (Allium sativum) on alloxan induced diabetic rabbit model

2016 ◽  
Vol 2 (3) ◽  
pp. 402-408
Author(s):  
Ambiara ◽  
Fahima Binthe Aziz ◽  
Md Mahmudul Hasan ◽  
Md Shajedur Rahman ◽  
Misrat Masuma Parvez ◽  
...  
Keyword(s):  
Body Weight ◽  
Glucose Level ◽  
Combined Treatment ◽  
Rabbit Model ◽  
Treated Group ◽  
Control Group ◽  
Bitter Melon ◽  
Diabetic Rabbit ◽  
Group A ◽  
Group B

The present study was undertaken to investigate the antidiabetic effect of the Bitter melon and Garlic on Alloxan induced diabetes in experimental rabbits. At 2 to 3 months of age, rabbits were assigned into five groups (A, B, C, D and E) and each group was remained 4 rabbits. Group A was kept for control, Group B was treated with Alloxanintramuscullarly at a dose of 75mg /kg body weight, Group C was treated with bitter melon 250gm/kg body weight orally, Group D was treated with garlic 750mg/kg body weight orally, Group E treated with combined at previous dose. After acclimatization, diabetes was induced in four groups of rabbits (B, C, D and E) by administering Alloxan injection in a dose of 75mg/kg body weight (b.wt.) intramuscularlly. There was significant decreased in blood glucose level in all treated group C, D, E compared to the B group and lowest glucose was recorded in E group when treated with combined medicinal herbs and body weight was increased in all treated group C, D, E compared to the B group and highest was recorded in Dgroup while treated with those.% of PCV level and Hb gm/dl concentration was the highest in group E which was treated with both garlic and bitter melon compare to the A group. ESR was highest in group B treated with Alloxan and lowest in group E. The present study reveals that combined treatment increases body weight and decreases glucose level without affecting health of rabbits.Asian J. Med. Biol. Res. September 2016, 2(3): 402-408

scholarly journals Effects of Dried Aloe Vera Gel and Diclofenac on Sodium and Potassium Homeostasis: An Experimental Study on Hypertensive Rats

2021 ◽  
Vol 10 (3) ◽  
pp. 133-139
Author(s):  
Nadeem Yaqoob ◽  
Abdul Qudoos Arain ◽  
Mufakhara Fatimah ◽  
Samina Kausar ◽  
Sadia Chiragh
Keyword(s):  
Experimental Study ◽  
Serum Potassium ◽  
Aloe Vera ◽  
Control Group ◽  
Hypertensive Rats ◽  
Aloe Vera Gel ◽  
Group A ◽  
Group B ◽  
Group D ◽  
Sodium And Potassium

Background: Anti-inflammatory role of Aloe vera gel is well established. Diclofenac is extensively used for acute and chronic inflammation. The present study was conducted to compare dried Aloe vera gel and diclofenac effects on sodium and potassium balance in hypertensive rats. Material and Methods: This experimental study was conducted at Sargodha Medical College from May to November 2016. Twenty-four healthy male Sprague Dawley rats 7-8 weeks of age were included in study. Any unhealthy-looking rat was excluded from the study. Rats were equally and randomly divided into four groups Normal control (group A), Model control (group B), Aloe vera (group C) & Diclofenac (group D). Hypertension was induced by a 20 % sucrose diet in all groups except group A in 8 weeks’ time. Group B, C & D received distilled water and Aloe vera dried gel 400 mg/kg & diclofenac powder 12 mg/kg body weight respectively orally between 8 to 10 weeks. Serum and urine analysis was performed for hematocrit, sodium, and potassium concentrations at zero, eight and ten weeks. Twenty-four-hour urinary sodium excretion was calculated. Data was analyzed using Graph Pad Prism version 6. Result: After 2-week administration of aloe vera and diclofenac powder, serum potassium significantly decreased in Group C (p <0.001) while increased in Group B and D (p <0.001) as compared to group A. Urinary sodium concentration and excretion increased significantly in Group C (p <0.01) as compared to Group A whereas result of Group D was insignificant. No significant change in serum sodium and hematocrit of any group was observed. Conclusion: Aloe vera causes less sodium retention than diclofenac but decreases serum potassium contrary to the effect of diclofenac in hypertensive rats.

Protective Effect of Thyroxine on Minocycline Induced Thyroid Gland Damage

2020 ◽  
Author(s):  
Tahira Assad
Keyword(s):  
Thyroid Gland ◽  
Concomitant Administration ◽  
Treated Group ◽  
Control Group ◽  
Similar Amount ◽  
Protective Effects ◽  
Absolute Weight ◽  
Beneficial Effects ◽  
Group A ◽  
Group B

Background: Thyroxine has shown beneficial effects on intelligence, learning, and memory process in patients of congenital hypothyroidism. Minocycline has been used in clinical practice for various indications and reported to have anti-thyroid effects. This study was specifically designed to observe the role of thyroxine on minocycline induced damage to thyroid gland. Methods: This experimental study was undertaken at Anatomy department of BMSI, JPMC, Karachi, for eight weeks, from October to November 2019. Thirty adult (10-12 months) male guinea pigs, weighing from 450-650 gm were obtained and divided into 3 groups. Group A served as control, group B was given Minocycline 0.02mg/gram/day once daily and group C was administered Minocycline in similar amount as group B along with thyroxine 0.5µg/gram/day for the same duration. Dosing was continued for 8 weeks, at the completion of which all the animals were sacrificed. Thyroid gland was processed and tissue sections were stained with Haematoxylin and Eosin for morphology. Results: The absolute weight of thyroid gland was significantly increased (p<0.001) in minocycline treated group B animals compared to the control animals, whereas substantial decrease (p<0.01) in absolute weight of thyroid gland was witnessed in group C in comparison to group B animals. The follicular cells showed hypertrophy and shrinkage of colloid in the thyroid follicles. These changes were prevented when animals were co-administered with thyroxine and minocycline in Group C. Conclusion: Concomitant administration of thyroxine with the antimicrobial drug minocycline showed protective effects of thyroxine on Minocycline induced damage to thyroid gland of animals.

The Role of Xylitol Gum Chewing in Restoring Postoperative Bowel Activity After Cesarean Section

2015 ◽  
Vol 18 (2) ◽  
pp. 167-172 ◽  
Author(s):  
Jian Tao Lee ◽  
Mei-Hui Hsieh ◽  
Po-Jen Cheng ◽  
Jr-Rung Lin
Keyword(s):  
Cesarean Section ◽  
Chewing Gum ◽  
Control Group ◽  
Bowel Sounds ◽  
Gum Chewing ◽  
Group A ◽  
The Times ◽  
Group B ◽  
Post Hoc

Aims: The goal of this study was to evaluate the effects of xylitol gum chewing on gastrointestinal recovery after cesarean section. Methods: Women who underwent cesarean section ( N = 120) were randomly allocated into Group A (xylitol gum), Group B (nonxylitol gum), or the control group (no chewing gum). Every 2 hr post-cesarean section and until first flatus, Groups A and B received two pellets of chewing gum and were asked to chew for 15 min. The times to first bowel sounds, first flatus, and first defecation were then compared among the three groups. Results: Group A had the shortest mean time to first bowel sounds (6.9 ± 1.7 hr), followed by Group B (8 ± 1.6 hr) and the control group (12.8 ± 2.5 hr; one-way analysis of variance, p < .001; Scheffe’s post hoc comparisons, p < .05). The gum-chewing groups demonstrated a faster return of flatus than the control group did ( p < .001), but the time to flatus did not differ significantly between the gum-chewing groups. Additionally, the differences in the time to first defecation were not significant. Conclusion: After cesarean section, chewing gum increased participants’ return of bowel activity, as measured by the appearance of bowel sounds and the passage of flatus. In this context, xylitol-containing gum may be superior to xylitol-free gum.

Protective role of caffeic acid phenethyl ester and erdosteine on activities of purine-catabolizing enzymes and level of nitric oxide in red blood cells of isoniazid-administered rats*

2008 ◽  
Vol 24 (8) ◽  
pp. 519-524 ◽  
Author(s):  
HR Yilmaz ◽  
E Uz ◽  
O Gökalp ◽  
N Özçelik ◽  
E Çiçek ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Red Blood Cells ◽  
Caffeic Acid ◽  
Blood Cells ◽  
Tap Water ◽  
Treated Group ◽  
Caffeic Acid Phenethyl Ester ◽  
Male Rats ◽  
Control Group

The aim of this experimental study was to investigate the possible role of nitric oxide (NO) and the activities of adenosine deaminase (ADA) and xanthine oxidase (XO) in the pathogenesis of isoniazid (INH)-induced oxidative damage in red blood cells (RBCs), and also to show the effect of caffeic acid phenethyl ester (CAPE) and erdosteine, antioxidants, in decreasing this toxicity. A total of 25 adult male rats were divided into four experimental groups as follows: control group ( n = 7), INH-treated group ( n = 6), INH + CAPE–treated group ( n = 6), and INH + erdosteine–treated group ( n = 6). INH, INH-CAPE, and INH-erdosteine–treated groups were treated orally with INH 50 mg/kg daily and with the tap water for 15 days. Control group was given only tap water. CAPE was intraperitoneally injected for 15 days at a dose of 10 μmol/kg. Erdosteine was treated orally for 15 days at a dose of 10 mg/kg/day. The injection of INH led to a significant increase in the activities of ADA, XO, and NO levels in RBCs of rats. Co-treatment with CAPE caused a significant decrease in the activities of ADA and XO and the levels of NO in RBCs. In addition, co-treatment with erdosteine caused a significant decrease in the activities of ADA and XO and the levels of NO in RBCs. The results of this study showed that ADA, XO, and NO may play an important role in the pathogenesis of INH-induced oxidative stress in RBCs. CAPE and erdosteine may have protective potential in this process and they may become a promising drug in the prevention of this undesired side effect of INH.

scholarly journals CELECOXIB

2018 ◽  
Vol 25 (01) ◽  
pp. 50-57
Author(s):  
Sadia Sundus ◽  
Nazia Qamar ◽  
Raheela Adil ◽  
Muhammad Faisal Fahim
Keyword(s):  
Body Weight ◽  
Relative Weight ◽  
Treated Group ◽  
Renal Tissue ◽  
The Body ◽  
Control Group ◽  
Albino Rats ◽  
Group A ◽  
Group B ◽  
General Architecture

Objective: To observe the absolute, relative weight of kidney and body weightof albino rats on celecoxib induced kidney with protection by lycopene. Study Design:Experimental study. Place and Duration of study: This study was conducted in BMSI (Anatomydepartment), JPMC, Karachi, from 4th May 2015 to 3rd June 2015. Materials and Methods: Fortyhealthy adult, male Albino rats, 90-120 days old, weighing 200-220gm was taken for the study.The rats were divided into 4 groups, Group A was control group, Group B receive Celecoxib 50mg/kg body weight orally, Group C receive Celecoxib 50 mg/kg body weight orally along withlycopene50 mg/kg body weight orally and Group D receive lycopene 50 mg/kg body weightorally for 30 days. At the end of study rats were sacrificed and renal tissue sections were stainedwith hematoxylin and eosin. Results: Markedly decreased weight was observed in rats takingcelecoxib. Slides which were stained with hematoxylin and eosinshowed general architecture ofrenal parenchyma, shape and arrangement of epithelial cells. Apoptosis, hemorrhage, necrosisand vacuolation seen in Celecoxib group, whereas renal architecture were ameliorated andreverted back in celecoxib along with lycopene receiving group. Conclusion: This studyconcludes that lycopene restored the body weight, absolute and relative kidney weight incelecoxib treated group.

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