Protective effects of vitamin C and selenium supplementation on methomyl-induced tissue oxidative stress in adult rats

AbstractHIV/AIDS related mortality has been dramatically reduced by the advent of antiretroviral therapy (ART). However, ART presents with associated adverse effects. One of such adverse effects is hepatotoxicity observed with nevirapine (NVP) containing ART. Since previous studies showed that NVP hepatotoxicity may be due to oxidative stress via generation of oxidative radicals, this study sought to evaluate the protective effects of antioxidants in alleviating NVP induced hepatotoxicity. Rats were divided into 6 groups with 8 animals per group and received doses of the antioxidants jobelyn (10.7 mg/kg/day), vitamin C (8 mg/kg/day), vitamin E (5 mg/kg/day) and/or NVP (6 mg/kg/day) for 60 days. The animals were sacrificed on day 61 by cervical dislocation, blood samples were collected for biochemical and hematological examination. The liver of the sacrificed animals was weighed and subjected to histopathological examination. There was a statistically significant (p<0.05) elevation in MDA level observed in the NVP group as compared with control. The results further showed non-significant decreases in the levels of MDA in the NVP plus antioxidant groups, except vitamin C, when compared with the NVP alone group. Vitamin E and Vitamin E plus C treated groups showed significantly (p<0.05) higher levels of SOD, CAT and GSH. The results also showed statistically significantly (p<0.05) lower levels of ALT and AST in the antioxidant treated groups There was an observed significantly (p<0.05) higher level of TP and urea in the antioxidant treated rats. A significantly (p<0.05) higher white blood cell count was observed in the antioxidant groups. Histopathological assessment of the liver extracted from the rats showed no visible pathology across the groups. Observations from this study suggest a potentially positive modulatory effect of antioxidants and may be indicative for the inclusion of antioxidants in nevirapine containing ART.

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Protective Effects of Quercetin and Vitamin C against Oxidative Stress-Induced Neurodegeneration

Journal of Agricultural and Food Chemistry ◽

10.1021/jf049243r ◽

2004 ◽

Vol 52 (25) ◽

pp. 7514-7517 ◽

Cited By ~ 128

Author(s):

Ho Jin Heo ◽

Chang Yong Lee

Keyword(s):

Oxidative Stress ◽

Vitamin C ◽

Protective Effects

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Intermittent hypobaric hypoxia-induced oxidative stress in rat erythrocytes: protective effects of vitamin E, vitamin C, and carnitine

Cell Biochemistry and Function ◽

10.1002/cbf.1344 ◽

2007 ◽

Vol 25 (2) ◽

pp. 221-231 ◽

Cited By ~ 34

Author(s):

S. Asha Devi ◽

R. Vani ◽

M. V. V. Subramanyam ◽

Shiva Shankar Reddy ◽

K. Jeevaratnam

Keyword(s):

Oxidative Stress ◽

Vitamin E ◽

Vitamin C ◽

Hypobaric Hypoxia ◽

Protective Effects ◽

Rat Erythrocytes ◽

Intermittent Hypobaric Hypoxia

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Study the Effect of Vitamins (C and E) on Oxidative Stress and Antioxidants Changes Induced by VCM in Male Rats

International Journal of Pharmaceutical Quality Assurance ◽

10.25258/ijpqa.11.3.20 ◽

2020 ◽

Vol 11 (03) ◽

pp. 430-434

Author(s):

Shaymaa J. Shamran ◽

Haider S. Jaffat

Keyword(s):

Oxidative Stress ◽

Vitamin E ◽

Vitamin C ◽

Male Rats ◽

Control Group ◽

Protective Effects ◽

Vancomycin Group ◽

Animal House ◽

Antioxidant Effects ◽

Oxidative Effect

The current study was designed to determine the antioxidant effects of vitamin C and vitamin E against oxidative stress induced by vancomycin in some antioxidants changes in the male rats. The study was conducted in the animal house of the Faculty of Science/University of Kufa for the period from April, 2018 to May, 2018 on 119 animals of male rats aged 2.5–3 months and the weight of 150-200 gm. Two experiments designed in this study addressed the first and two experiments to study the oxidative effect of vancomycin in addition to the protective effects of vitamin C and vitamin E to reduce these effects in the treatment of animals for one week and three weeks with vancomycin and vancomycin plus vitamins. The results indicated a significant increase (p less than 0.05) in the MDA, CAT, and significant decrease (p less than 0.05) in SOD, and GPX. In the animals treated with vancomycin 40,60 mg/kg only compared to the control group for the two periods of administration at the same time occur a significant decrease(p less than 0.05) in the MDA, CAT and a significant increase (p less than 0.05) in the SOD and GPX after treated animals with vancomycin 40,60 mg/kg with vitamin C and vitamin E for a period of one and three weeks compared with vancomycin group.

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Protective Effects of Vitamin C Concomitant Treatment on Deferasirox-induced Renal Toxicity in Rats

Journal of Arak University of Medical Sciences ◽

10.32598/jams.23.6.62.7 ◽

2021 ◽

Vol 23 (6) ◽

pp. 926-943

Author(s):

Taha Fereydouni ◽

◽

Saeed Hajihashemi ◽

Parsa Yousefichaijan ◽

Ali Rahbari ◽

...

Keyword(s):

Oxidative Stress ◽

Vitamin C ◽

Creatinine Clearance ◽

Renal Toxicity ◽

Renal Tissue ◽

Beta Thalassemia ◽

Renal Tubules ◽

Concomitant Treatment ◽

Protective Effects ◽

Antioxidant Power

Background and Aim: Deferasirox (Exjade) is an iron-chelating drug used in patients with beta-thalassemia major. Oxidative stress is among f the major causes of nephrotoxicity and its progression. Deferasirox, due to oxidative stress and increased cell apoptosis causes the dysfunction of renal tubules and renal toxicity. According to its antioxidant and anti-inflammatory properties, the present study explored the effect of vitamin C on deferasirox-induced kidney damage. Methods & Materials: This study was performed on 30 Wistar rats in 3 groups of control, deferasirox, and deferasirox plus vitamin C. To induce the nephrotoxicity, the intra-peritoneum injection of deferasirox (75 mg/kg/day) was used. After taking plasma from the blood samples of the explored rats, we determined the values of Cr, Na+, K+, Mg+, osmolality, and BUN in the obtained plasma and urine samples. The creatinine clearance, as well as the relative and absolute excretion of sodium and potassium, were also calculated. After separating the two kidneys, they were used for the histologic study with Hematoxylin and Eosin (H&E) staining, as well as Malondialdehyde (MDA) and Ferric Reducing Antioxidant Power (FRAP) biochemical studies. Ethical Considerations This study was approved by the Research Ethics Committee of Arak University of Medical Sciences (Code: IR.ARAKMU.REC.1396.309). Results: Cotreatment with deferasirox and vitamin C reduced renal tissue MDA and relative and absolute Na and K excretion and urine osmolarity; this method also increased creatinine clearance and renal tissue FRAP. Conclusion: The co-administration of vitamin C presented a significant protective effect on the renal toxicity induced by deferasirox. The protective property of deferasirox is because of the antioxidant impacts of vitamin C in reducing oxidative stress and lipid peroxidation.

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Protective effects of selenium on methimazole-induced anemia and oxidative stress in adult rats and their offspring

Human & Experimental Toxicology ◽

10.1177/0960327110392403 ◽

2010 ◽

Vol 30 (10) ◽

pp. 1549-1560 ◽

Cited By ~ 10

Author(s):

Ibtissem Ben Amara ◽

Ahmed Hakim ◽

Afef Troudi ◽

Nejla Soudani ◽

Fatma Ayadi Makni ◽

...

Keyword(s):

Oxidative Stress ◽

Antithyroid Drug ◽

Diet Group ◽

Hematological Parameter ◽

Standard Diet ◽

Protective Effects ◽

Adult Rats ◽

Lactate Dehydrogenase Activity ◽

Group I ◽

Standard Diet Group

The present study investigates the potential ability of selenium, considered as an antioxidant with pharmacological property to alleviate oxidative stress and hematological parameter disorders induced by methimazole, an antithyroid drug. Pregnant Wistar rats were randomly divided into four groups of six each: group I served as negative control and received a standard diet; group II received 250 mg/L of methimazole in drinking water and a standard diet; group III received both methimazole (250 mg/L, orally) and selenium (0.5 mg/kg of diet) supplemented to the standard diet; group IV served as positive control and received a supplement of selenium in the diet (0.5 mg/kg of diet) as sodium selenite (Na2SeO3). Treatment was started from the 14th day of pregnancy until day 14 after delivery. Methimazole reduced the number of red blood cells, hemoglobin concentration and hematocrit in mothers and their pups. Besides, plasma iron, vitamins B9, B12, C and E levels were reduced. Lipid peroxidation increased, objectified by high malondialdehyde levels and lactate dehydrogenase activity in plasma, while glutathione, glutathione peroxidase, superoxide dismutase and catalase activities showed a significant decline. Co-administration of selenium through diet improved all the parameters cited above. It can be concluded that the administration of selenium alleviates methimazole-induced toxicity, thus demonstrating its antioxidant efficacy.

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PROTECTIVE EFFECTS OF VITAMIN C AGAINST OXIDATIVE STRESS ON BIRTH WEIGHT

Epidemiology ◽

10.1097/00001648-200407000-00211 ◽

2004 ◽

Vol 15 (4) ◽

pp. S85-S86 ◽

Cited By ~ 1

Author(s):

H Park ◽

B Park ◽

Y Kim ◽

H Lee ◽

E Park ◽

...

Keyword(s):

Oxidative Stress ◽

Birth Weight ◽

Vitamin C ◽

Protective Effects

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Vitamin C Protected Human Retinal Pigmented Epithelium from Oxidant Injury Depending on Regulating SIRT1

The Scientific World JOURNAL ◽

10.1155/2014/750634 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8 ◽

Cited By ~ 9

Author(s):

Wei Wei ◽

Langen Li ◽

Yufeng Zhang ◽

Geriletu ◽

Jia Yang ◽

...

Keyword(s):

Oxidative Stress ◽

Vitamin C ◽

Stress Responses ◽

Sirtuin 1 ◽

Protective Effects ◽

Combined Application ◽

Intracellular Ros ◽

Antioxidative Stress ◽

Sirt1 Activator ◽

Vit C

The purpose was to investigate the protective effects of Vitamin C (Vit C) and the regulatory mechanism between Vit C and sirtuin 1 (SIRT1) in PREs during oxidative stress as Vit C and SIRT1 exerted famous effects as antioxidants. We found that moderate Vit C (100 µM) prevented ARPE-19 cells from damages induced by H2O2, including increasing viability, reducing apoptosis, and attenuating intracellular ROS levels. But lower and higher concentration of Vit C had no effects. Further results indicated that Vit C caused the dysregulation of some stress responses factors (SIRT1, p53 and FOXO3) in ARPE-19 cells response to H2O2. Moreover we found that SIRT1 activator resveratrol (SRV) stimulated significantly the protective effects of moderate Vit C, provided the property of antioxidative stress for the lower and higher concentration of Vit C in ARPE-19 cells as well. Consistently, nicotinamide (NA) relieved the protective functions of moderate Vit C. Interestingly, data also revealed the dysregulation of p53 and FOXO3 was dependent on the regulation of SIRT1 rather than Vit C. Summarily, the protective effect of Vit C against oxidative stress was involved in regulation of SIRT1. It suggested that combined application of Vit C and RSV might be a promising therapeutic method for AMD.

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Prepubertal Exposure to Genistein Alleviates Di-(2-ethylhexyl) Phthalate Induced Testicular Oxidative Stress in Adult Rats

BioMed Research International ◽

10.1155/2014/598630 ◽

2014 ◽

Vol 2014 ◽

pp. 1-9 ◽

Cited By ~ 7

Author(s):

Lian-Dong Zhang ◽

He-Cheng Li ◽

Tie Chong ◽

Ming Gao ◽

Jian Yin ◽

...

Keyword(s):

Oxidative Stress ◽

Body Weight ◽

Protective Effects ◽

Sprague Dawley ◽

Adult Rats ◽

Environmental Endocrine Disruptors ◽

Sprague Dawley Rats ◽

Antioxidative Effects ◽

Testicular Histology ◽

Ethylhexyl Phthalate

Di-(2-ethylhexyl) phthalate (DEHP) is the most widely used plastizer in the world and can suppress testosterone production via activation of oxidative stress. Genistein (GEN) is one of the isoflavones ingredients exhibiting weak estrogenic and potentially antioxidative effects. However, study on reproductive effects following prepubertal multiple endocrine disrupters exposure has been lacking. In this study, DEHP and GEN were administrated to prepubertal male Sprague-Dawley rats by gavage from postnatal day 22 (PND22) to PND35 with vehicle control, GEN at 50 mg/kg body weight (bw)/day (G), DEHP at 50, 150, 450 mg/kg bw/day (D50, D150, D450) and their mixture (G + D50, G + D150, G + D450). On PND90, general morphometry (body weight, AGD, organ weight, and organ coefficient), testicular redox state, and testicular histology were studied. Our results indicated that DEHP could significantly decrease sex organs weight, organ coefficient, and testicular antioxidative ability, which largely depended on the dose of DEHP. However, coadministration of GEN could partially alleviate DEHP-induced reproductive injuries via enhancement of testicular antioxidative enzymes activities, which indicates that GEN has protective effects on DEHP-induced male reproductive system damage after prepubertal exposure and GEN may have promising future in its curative antioxidative role for reproductive disorders caused by other environmental endocrine disruptors.

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